TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can ...read more
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - The TLR4 reporter cell line responds to LPS. The TLR2 / TLR4 inhibitor specifically inhibits TLR4 activation upon LPS stimulation in a dose-response manner.
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 /4 Inhibitor suppressed the Pam3CSK4-induced TLR2/TLR1 activity [A] and the LPS-induced TLR4 activity [B] in a dose-response manner, of which IC50 values were ...read more
Antennapedia Control Peptide: 1 mg (lyophilized); sequence: DRQIKIWFQNRRMKWKK; Molecular weight: 2361 Da
TLR2 and TLR4 Inhibitor Peptide: COBRA TLR2 and TLR4 Inhibitor interferes with interaction between TIRAP/Mal and TIR domain of TLR2 or TLR4.
Preparation of 2.5 mM peptide stock solutions
TLR2 / TLR4 Inhibitor Peptide: A final volume of 100 ul will make a 2.5 mM stock solution. Add 100 ul sterile water to the tube of peptide. Carefully pipet to ensure all of the peptide is dissolved and briefly spin the tube before opening.
Antennapedia Control Peptide: A final volume of 170 ul will make a 2.5 mM stock solution. Add 170 ul sterile water to the tube of peptide. Carefully pipet to ensure all of the peptide is dissolved and briefly spin the tube before opening.
The stock solutions may be diluted further to make working solutions. Dilute according to the needs for your assay. For example, dilute 2.5 mM stock solutions 1:10 in sterile 1X PBS or cell culture media to make 250 uM working solutions. Working solution should be made fresh daily and not be stored.
The inhibitor is used in assays to inhibit TLR2 and TLR4 signaling. We recommend an initial titration of the inhibitor from 0-50 uM for in vitro assays along with control of which concentrations should be mirror inhibitor concentrations. Inhibitor and control should be preincubated with cells prior to ligand activation to allow sufficient time for the peptides to enter from the media into the cell. We typically preincubate with inhibitor and control for 1 h prior to TLR2 or TLR4 activation (Figures 1 and 2); however, optimal preincubation times may vary between model systems. The TLR2/NF-kB/SEAPorter™ cell line and TLR4/MD2/CD14/NF-kB/SEAPorter™ cell line are a useful positive control model system for studying inhibition of TLR2 and TLR4 activation, respectively (Figures 1 through 3). Use in In vitro assay reported in scientific literature (PMID 26908090). Use in FLOW cytometry reported in scientific literature (PMID 27838489).
Read Publications using NBP2-26245 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Form: White solid Solubilize the peptides prior to use by preparing 2.5 mM stock solution in sterile water.
Alternate Names for TIRAP (TLR2 and TLR4) Inhibitor Peptide Set
adapter protein wyatt
Adaptor protein Wyatt
MyD88 adapter-like protein
TIR domain-containing adapter protein
toll/interleukin-1 receptor domain-containing adapter protein
toll-interleukin 1 receptor (TIR) domain containing adaptor protein
Toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
Toll-like receptor adaptor protein
TIRAP/Mal is an adapter protein in the signaling pathways activated by TLR2 and TLR4, and appears to be essential for MyD88-dependent TLR2 and TLR4 signaling pathways. TIRAP is recruited to activated TL2 and TLR4 through interaction with TIR domain of the receptor. This peptide contains a sequence from mouse TIRAP that blocks the function of TIRAP, likely through binding to the receptor and blocking TIR-TIR domain interaction between TIRAP and the receptor.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.
FAQs for TIRAP (TLR2 and TLR4) Inhibitor (NBP2-26245). (Showing 1 - 2 of 2 FAQs).
What is the advantage of NBP2-26245 over NBP2-29331? Both of them are TIRAP Inhibitory Peptides but I do not see in the literature provided in the webepage why I would choose one over the other? Or why would I spend more on NBP2-26245?
While both of these peptides serve the same function, there are differences in the functional sequence. I would like to direct you to to two attached articles in regards to this. 1. NBP2-26245 (Piao, 2013), sequence is shown in Figure 3G, labelled as mTIRAP 2. NBP2-29331: (Shilling 2002), sequence is described under Reagents and Abs on page 5875. All things aside, we have extensive testing data for NBP2-26245 from in-house testing, whereas we are still in the process of validating NBP2-29331 in-house. We can offer much better support for NBP2-26245 so this is the product I would recommend.
Our customer has a question about NBP2-26245. Inhibitors are guaranteed for 1 year from date of receipt is described on the product page. However, the customer said that their project may last longer, and they cannot ensure when the product will be applied. Would you please help check and suggest the expiry date of the newest lot of NBP2-26245?
We do not state expiry dates on our products however, as you rightly point out, our TIRAP (TLR2 and TLR4) inhibitor peptide set with catalogue number NBP2-26245 is guaranteed for 1 year from the date of receipt. Upon receipt of the product I recommend that your customer stores it in its lyophilised form at -20C or -80C until they actually need to use it, since lyophilised products are more stable than liquids. Upon reconstitution they should aliquot the product and store it at -20C or -80C, avoiding freeze-thaw cycles. We do not have any testing data regarding the stability of this product beyond a year and cannot guarantee it beyond this time.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.