Mre11 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Mre11 Antibody [NB100-142] - IF analysis of Mre11 on HeLa. Image from verified customer review.
Immunohistochemistry: Mre11 Antibody [NB100-142] - Immunohistochemical staining of MRE11 in a human epidermis cross section.
Simple Western: Mre11 Antibody [NB100-142] - Image shows a specific band for Mre11 in 0.5 mg/mL of HeLa lysate. A band appears at the theoretical molecular weight of 81 kDa for this product. This experiment was ...read more
Genetic Strategies: Knockdown Validated: Mre11 Antibody [NB100-142] - TIG-1 human primary fibroblasts, whole cell lysate (30 ug). Image from verified customer review.
Western Blot: Mre11 Antibody [NB100-142] - Western blot analysis of Mre11 on 50 ug of HeLa and MEF lysates, displaying bands at the molecular weight of 81 kDa.
Immunocytochemistry/ Immunofluorescence: Mre11 Antibody [NB100-142] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Ha, Bt, Bv, Ca, Pm, Eq, Fe, PmSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Mre11 Antibody Summary

Immunogen
Mre11 Antibody is made to a full length human Mre11 protein. [Uniprot: P49959]
Predicted Species
Bovine (92%), Chimpanzee (100%), Feline (94%), Canine (94%), Bat (92%), Primate (100%), Equine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MRE11
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Simple Western 1:250
  • Chromatin Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 3 uL
  • Knockdown Validated
  • Knockout Validated
Application Notes
Use in ICC/IF (PMID: 26774475), Immunohistochemistry-Paraffin (PMID: 21279473), and immunoblotting (PMID: 28115467).reported in scientific literature. In Western blot, a band can be seen at ~ 81 kDa. For ICC/IF, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. For IP, the suggested working dilution is 3 ul for immunoprecipitation of 3X10^6 cells. Co-IP application has been reported in scientific literature (PMID: 22190719). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24349281) In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockout validation (PMID: VanCevska et al.). Use in ELISA reported in scientific literature (PMID: 16788144).
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Mre11 Overexpression Lysate
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB100-142 in the following applications:

Publications
Read Publications using
NB100-142 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Chimpanzee (100%), Gibbon (99%), Marmoset (96%), Canine (94%), Feline (94%), Panda (94%), Equine (92%), Bovine (92%), Bat (92%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Mre11 Antibody

  • ATLDmeiotic recombination (S. cerevisiae) 11 homolog A
  • AT-like disease
  • DNA recombination and repair protein
  • double-strand break repair protein MRE11A
  • endo/exonuclease Mre11
  • HNGS1
  • Meiotic recombination 11 homolog 1
  • Meiotic recombination 11 homolog A
  • MRE11 homolog 1
  • MRE11 homolog A
  • MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
  • Mre11
  • MRE11A
  • MRE11B

Background

Mre11 (double-strand break repair protein MRE11A) is an important component of MRN/X complex (Mre11, Rad50, and Nbs1/Xrs2), playing a central role in double-strand break (DSB) repair, DNA recombination, telomere integrity maintenance, meiotic cell division, and viral infection. Mre11 and Rad50 comprise the catalytic component of the MRN complex with Rad50 involved in ATP hydrolysis and Mre11 functioning as a nuclease having both single-strand endonuclease and double-strand-specific 3'-5' exonuclease activity (1). The MRN complex is also involved in DNA damage signaling via activation of ATM kinase and in telomeres, where it may modulate t-loop formation (2). In addition, Mre11 is a component of the BASC complex (BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN) and interacts with DCLRE1C/Artemis as well as DCLRE1B/Apollo (3).

The MRN assembles as a hetero-hexamer consisting of 2 RAD50 subunits, 2 MRE11 nucleases, and 2 NBS1 subunits, specific to eukaryotes. Mre11 is highly conserved with the N-terminal region of the human protein sharing approximately 50% amino acid sequence identity with the yeast ortholog. Human Mre11 has 3 known isoforms with the predominant isoform having a theoretical molecular weight of 80.6kDa. This nuclear localized protein has high expression in proliferating tissues. Defects in Mre11 can lead to genomic instability, telomere shortening, aberrant meiosis, hypersensitivity to DNA damage, and ataxia telangiectasia-like disorder (ATLD). MRN expression has been associated with the prognosis of cancer and MRN mutants have been linked to cancer susceptibility in ovarian cancer and glioma (4, 5).

References

1. Paull TT. (2018) 20 Years of Mre11 Biology: No End in Sight. Mol Cell. 71(3):419-427. PMID: 30057197

2. Syed A, Tainer JA. (2018) The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair. Annu Rev Biochem. 87:263-294. PMID: 29709199

3. Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J. (2000) BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 14(8):927-39. PMID: 10783165

4. Kim JH, Grosbart M, Anand R, Wyman C, Cejka P, Petrini JHJ. (2017) The Mre11-Nbs1 Interface Is Essential for Viability and Tumor Suppression. Cell Rep. 18(2):496-507. PMID: 28076792

5. Bian L, Meng Y, Zhang M, Li D. (2019) MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment. Mol Cancer. 18(1):169. PMID: 31767017

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-142
Species: Hu, Mu, Rt, Ch, Ha, Bt, Bv, Ca, Pm, Eq, Fe, Pm
Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO

Publications for Mre11 Antibody (NB100-142)(181)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Hamster, Virus.

We have publications tested in 10 applications: ChIP, FLOW, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Knockout Validated, WB.


Filter By Application
ChIP
(6)
FLOW
(1)
IB
(1)
ICC/IF
(43)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
IP
(14)
Knockout Validated
(1)
WB
(73)
All Applications
Filter By Species
Human
(91)
Mouse
(21)
Rat
(2)
Chicken
(1)
Hamster
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 181. Show All 181 Publications.
Publications using NB100-142 Applications Species
Volcic M, Sparrer KMJ, Koepke L et al. Vpu modulates DNA repair to suppress innate sensing and hyper-integration of HIV-1 Nat Microbiol Jul 20 2020 [PMID: 32690953] (WB, Human) WB Human
VanCevska A, Pfeiffer V, Feretzaki M, Ahmed W SMCHD1 Promotes ATM-dependent DNA Damage Signaling and Repair of Uncapped Telomeres bioRxiv (WB, Knockout Validated, Human) WB, Knockout Validated Human
Hanlon KS, Meltzer JC, Buzhdygan T et al. Selection of an Efficient AAV Vector for Robust CNS Transgene Expression Mol Ther Methods Clin Dev Dec 13 2019 [PMID: 31788496]
Scherthan H, Lee JH, Maus E et al. Nanostructure of Clustered DNA Damage in Leukocytes after In-Solution Irradiation with the Alpha Emitter Ra-223 Cancers (Basel) Nov 26 2019 [PMID: 31779276] (ICC/IF, Human) ICC/IF Human
Fievet A, Bellanger D, Zahed L et al. DNA repair functional analyses of NBN hypomorphic variants associated with NBN-related infertility Hum Mutat. Nov 15 2019 [PMID: 31729086] (WB, Human) WB Human
Yoshimura Y Increased error-free DNA repair gene expression through reprogramming in human iPS cells Regenerative Therapy 2019 Jun 28 [PMID: 31304203] (WB, Human) WB Human
Velichko AK, Petrova NV, Luzhin AV et al. Hypoosmotic stress induces R loop formation in nucleoli and ATR/ATM-dependent silencing of nucleolar transcription Nucleic Acids Res. May 22 2019 [PMID: 31114877] (ChIP, Human, Mouse) ChIP Human, Mouse
Afzal S, Garg S, Ishida Y et al. Rat Glioma Cell-Based Functional Characterization of Anti-Stress and Protein Deaggregation Activities in the Marine Carotenoids, Astaxanthin and Fucoxanthin Mar Drugs. 2019 Mar 22 [PMID: 30909572] (WB, Rat) WB Rat
Guo Y, Gabola M, Lattanzio R et al. Loss of cyclin A2 in murine colonic epithelial cells disrupts colon homeostasis by triggering DNA damage and dysplasia and high cyclin A2 expression is a good-prognosis factor in patients with colorectal cancer bioRxiv (ICC/IF, Mouse) ICC/IF Mouse
Pan X, Chen Y, Biju B et al. FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops Sci Rep Dec 13 2019 [PMID: 31836759] (WB, Human) WB Human
Show All 181 Publications.

Reviews for Mre11 Antibody (NB100-142) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NB100-142:
Filter by Applications
IF
(3)
WB
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 12/12/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedtestes
SpeciesMouse

Blocking

Blocking DetailsSee PMID 20927532

Primary Anitbody

Dilution Ratio1:200 dilution

Details

Detection NotesSee PMID 20927532

Comments

CommentsPublished in PMID: 20927532
 
reviewed by:
Barbara Alcaraz Silva
IF Other 10/08/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedMammalian
SpeciesOther
LotN-2

Primary Anitbody

Dilution Ratio1:500

Details

Fixation Details4% PFA, RT
Immunofluorescence Mre11 NB100-142
Enlarge
reviewed by:
Svetlana Khoronenkova
IF Human 07/31/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
Lotno lot number on the tube

Blocking

Blocking Details2% BSA in PBS, 1 h at RT

Primary Anitbody

Dilution Ratio1:500 in 2% BSA/PBS, 14h at 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#A-11008 from Life Technologies (by Molecular Probes)
Secondary Concentration0.2 ug/ml

Details

Fixation Details4% Paraformaldehyde as fixative, permeabilization with 0.2% Triton-X100
Wash Description3x washes in PBS for 5 min
Western Blot Mre11 NB100-142
Enlarge
reviewed by:
Svetlana Khoronenkova
WB Human 04/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedTIG-1 human primary fibroblasts, whole cell lysate (30 ug)
SpeciesHuman
Lotno lot number on the tuve

Blocking

Blocking DetailsLI-COR® Odyssey® Blocking Buffer for 45 min at RT

Primary Anitbody

Dilution Ratio1:1000 for 1h at RT in LI-COR® Odyssey® Blocking Buffer 1:1 in PBS + 0.1%Tween

Secondary Antibody

Secondary DescriptionAlexa Fluor® 680 Goat Anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#A-21076 from Life Technologies (by Molecular Probes)
Secondary Concentration0.2 ug/ml

Details

Detection NotesLI-COR® Odyssey® detection system, Mre11 siRNA as a negative control

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Mre11 Antibody (NB100-142). (Showing 1 - 2 of 2 FAQs).

  1. Could you recommend an optimal Mre11 positive control for Western blot among your products?
    • NBL1-13219 would be the best product since it is intended for this purpose.
  2. I would like to know if these antibodies are suitable with IHC techniques using HRP conjugated antibodies and DAB as chromogen? Cat# NB200-175, NB100-904 and NB100-142
    • Yes, all of these antibodies are useful for IHC-P with DAB based detection. Please note that the PTEN Antibody (28H6) NB200-175 has been discontinued, however.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Mre11.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

 
Anonymous
12/12/2014
Application: IF
Species: Mouse

 
Barbara Alcaraz Silva
10/08/2014
Application: IF
Species: Other

Svetlana Khoronenkova
07/31/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol MRE11
Entrez
Uniprot