Mre11 Antibody

Images

 
Western Blot: Mre11 Antibody [NB100-142] - Analysis of Mre11 on 50 ug of HeLa and MEF lysates, using NB100-142.
Immunocytochemistry/ Immunofluorescence: Mre11 Antibody [NB100-142] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry: Mre11 Antibody [NB100-142] - Staining of MRE11 in a human epidermis cross section.
Western Blot: Mre11 Antibody [NB100-142] - TIG-1 human primary fibroblasts, whole cell lysate (30 ug). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Mre11 Antibody [NB100-142] - IF on HeLa. Image from verified customer review.
Simple Western: Mre11 Antibody [NB100-142] - Simple Western lane view shows a specific band for Mre11 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, HaSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Mre11 Antibody Summary

Immunogen
Full length human Mre11 protein. [UniProt# P49959]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MRE11A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Simple Western 1:250
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 3 ul
Application Notes
This Mre11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections (PMID 21279473). Use in Immunoblotting reported in scientific literature (PMID 28115467). Immunoprecipitation and Western Blot. In WB, a band can be seen at approx. 81 kDa. For ICC/IF, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. For IP, the suggested working dilution is 3 ul for immunoprecipitation of 3X10^6 cells. Co-IP application has been reported by Ching et al 2012 (PMID: 22190719). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24349281)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Mre11 Lysate (NBL1-13219)
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB100-142 in the following applications:

Publications
Read Publications using
NB100-142 in the following applications:

  • 4 publications
  • 1 publication
  • IB
    1 publication
  • 37 publications
  • IHC
    4 publications
  • 1 publication
  • 1 publication
  • IP
    13 publications
  • WB
    57 publications

Reactivity Notes

Hamster, human, chicken, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Mre11 Antibody

  • ATLDmeiotic recombination (S. cerevisiae) 11 homolog A
  • AT-like disease
  • DNA recombination and repair protein
  • double-strand break repair protein MRE11A
  • endo/exonuclease Mre11
  • HNGS1
  • Meiotic recombination 11 homolog 1
  • Meiotic recombination 11 homolog A
  • MRE11 homolog 1
  • MRE11 homolog A
  • MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
  • Mre11
  • MRE11A
  • MRE11MRE11B

Background

Mre11 (double-strand break repair protein MRE11A) is an important component of MRN complex (Mre11-hRad50-NBS1) which plays a central role in double-strand breaks (DSBs) repair, DNA recombination, telomere integrity maintenance and meiotic cell division. MRN complex has single-strand endonuclease as well as double-strand-specific 3'-5' exonuclease activities that are contributed by MRE11A. RAD50 binds DNA ends and hold them in close proximity which expedite searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation. MRN complex can also involve in DNA damage signaling via activation of ATM kinase and in telomeres, where it may modulate t-loop formation. Mre11 is also a component of the BASC complex (BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN) and interacts with DCLRE1C/Artemis as well as DCLRE1B/Apollo. Defects in MRE11 can lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage and ataxia telangiectasia-like disorder (ATLD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-142
Species: Hu, Mu, Rt, Ch, Ha
Applications: WB, Simple Western, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Mre11 Antibody (NB100-142)(152)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Hamster.

We have publications tested in 9 applications: ChIP, FLOW, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ChIP
(4)
FLOW
(1)
IB
(1)
ICC/IF
(37)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
IP
(13)
WB
(57)
All Applications
Filter By Species
Human
(71)
Mouse
(17)
Rat
(1)
Chicken
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 152. Show All 152 Publications.
Publications using NB100-142 Applications Species
Morales AJ, Carrero JA, Hung PJ et al. A type I IFN-dependent DNA damage response regulates the genetic program and inflammasome activation in macrophages. Elife 2017 Mar 31 [PMID: 28362262] (WB) WB
Gong Y, Handa N, Kowalczykowski SC, de Lange T. PHF11 promotes DSB resection, ATR signaling, and HR Genes Dev Jan 1 2017 [PMID: 28115467] (IB, Mouse) IB Mouse
Speiseder T, Hofmann-Sieber H, Rodriguez E et al. Efficient transformation of primary human mesenchymal stromal cells by adenovirus early region 1 oncogenes. J. Virol. Oct 19 2016 [PMID: 27795433] (WB, Human) WB Human
Cirilli M, Bereshchenko O, Ermakova O, Nerlov C Insights into specificity, redundancy and new cellular functions of C/EBPa and C/EBPb transcription factors through interactome network analysis. Biochim. Biophys. Acta Oct 14 2016 [PMID: 27746211] (WB, Mouse) WB Mouse
Xu Z, Song Z, Li G et al. H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation. Nucleic Acids Res 2016 Jul 18 [PMID: 27431324]
Hampp S, Kiessling T, Buechle K et al. DNA damage tolerance pathway involving DNA polymerase L and the tumor suppressor p53 regulates DNA replication fork progression Proc. Natl. Acad. Sci. U.S.A. 2016 Jul 26 [PMID: 27407148] (WB) WB
Choi YE, Meghani K, Brault ME et al. Platinum and PARP Inhibitor Resistance Due to Overexpression of MicroRNA-622 in BRCA1-Mutant Ovarian Cancer. Cell Rep. 2016 Jan 26 [PMID: 26774475] (ICC/IF, Human)

Details:
Mre11 antibody (NB100-142) was used for ICC/IF analysis of RPE1 Fucci cells which were transfected with control ANT or miR-622 ANT and irradiated with 5 Gy IR for 3 hours (Figure 5).
ICC/IF Human
Mayer A, Baran V, Sakakibara Y et al. DNA damage response during mouse oocyte maturation. Cell Cycle. 2016 Jan 08 [PMID: 26745237] (ICC/IF, Human) ICC/IF Human
Obermeier K, Sachsenweger J, Friedl TW et al. Heterozygous PALB2 c.1592delT mutation channels DNA double-strand break repair into error-prone pathways in breast cancer patients. Oncogene. 2015 Dec 07 [PMID: 26640152] (WB, Human) WB Human
Burck C, Mund A, Berscheminski J et al. KAP1 is a host restriction factor that promotes HAdV E1B-55K SUMO modification. J. Virol. 2015 Nov 04 [PMID: 26537675] (Human) Human
Show All 152 Publications.

Reviews for Mre11 Antibody (NB100-142) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NB100-142:
Filter by Applications
IF
(3)
WB
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 12/12/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedtestes
SpeciesMouse

Blocking

Blocking DetailsSee PMID 20927532

Primary Anitbody

Dilution Ratio1:200 dilution

Details

Detection NotesSee PMID 20927532

Comments

CommentsPublished in PMID: 20927532
 
reviewed by:
Barbara Alcaraz Silva
IF Other 10/08/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedMammalian
SpeciesOther
LotN-2

Primary Anitbody

Dilution Ratio1:500

Details

Fixation Details4% PFA, RT
Immunofluorescence Mre11 NB100-142
Enlarge
reviewed by:
Svetlana Khoronenkova
IF Human 07/31/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
Lotno lot number on the tube

Blocking

Blocking Details2% BSA in PBS, 1 h at RT

Primary Anitbody

Dilution Ratio1:500 in 2% BSA/PBS, 14h at 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#A-11008 from Life Technologies (by Molecular Probes)
Secondary Concentration0.2 ug/ml

Details

Fixation Details4% Paraformaldehyde as fixative, permeabilization with 0.2% Triton-X100
Wash Description3x washes in PBS for 5 min
Western Blot Mre11 NB100-142
Enlarge
reviewed by:
Svetlana Khoronenkova
WB Human 04/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedTIG-1 human primary fibroblasts, whole cell lysate (30 ug)
SpeciesHuman
Lotno lot number on the tuve

Blocking

Blocking DetailsLI-COR® Odyssey® Blocking Buffer for 45 min at RT

Primary Anitbody

Dilution Ratio1:1000 for 1h at RT in LI-COR® Odyssey® Blocking Buffer 1:1 in PBS + 0.1%Tween

Secondary Antibody

Secondary DescriptionAlexa Fluor® 680 Goat Anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#A-21076 from Life Technologies (by Molecular Probes)
Secondary Concentration0.2 ug/ml

Details

Detection NotesLI-COR® Odyssey® detection system, Mre11 siRNA as a negative control

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Mre11 Antibody (NB100-142). (Showing 1 - 1 of 1 FAQs).

  1. I would like to know if these antibodies are suitable with IHC techniques using HRP conjugated antibodies and DAB as chromogen? Cat# NB200-175, NB100-904 and NB100-142
    • Yes, all of these antibodies are useful for IHC-P with DAB based detection. Please note that the PTEN Antibody (28H6) NB200-175 has been discontinued, however.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Mre11 Antibody (NB100-142)

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Blogs on Mre11.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

 
Anonymous
12/12/2014
Application: IF
Species: Mouse

 
Barbara Alcaraz Silva
10/08/2014
Application: IF
Species: Other

Svetlana Khoronenkova
07/31/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol MRE11A
Entrez
Uniprot