Immunofluorescence Procedure
A 5beta in situ extraction method [10mM Pipes, pH 6.8 / 0.2% Triton X-100 / 100mM MgCl2 / 100mM sucrose/ 10mM EGTA Beta on ice] followed by 4% paraformaldehyde fixation of tissues works well for immunofluorescence of anti-hMre11 (NB 100-142).
Please see reference: Franchitto, A., Pichierri, P., Blooms syndrome protein is required for correct relocalization of RAD50/Mre11/nbs1 complex after replication fork arrest. J. of Cell Biology, DOI: 10 (2002)
