Artemin Antibody [Unconjugated]


Recombinant Mouse Artemin (Catalog # 1085-AR) stimulates proliferation in the SH‑SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC, B/N

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Artemin Antibody [Unconjugated] Summary

E. coli-derived recombinant mouse Artemin
Accession # Q9Z0L2
Detects mouse Artemin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human GDNF and recombinant rat GDNF is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.1-0.3 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF1085 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Artemin Antibody [Unconjugated]

  • Artemin
  • ARTN
  • Enovin
  • EVN
  • EVNneurotrophic factor
  • NBN
  • Neublastin


Artemin is a member of the Glia Cell-Derived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor beta (TGF‑ beta ) superfamily (1‑4). Similar to other TGF‑ beta family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide‑linked homodimer of the carboxy-terminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands (5). Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha 1‑GFR alpha 4) and a common signaling component, cRET (receptor tyrosine kinase) (5‑8). Artemin prefers to bind to GFR alpha 3 and activites the GFR alpha 3‑RET. However, in the presence of RET, it can bind to GFR alpha 1 as well (4, 5, 9). Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs (5, 10, 11).

  1. Lin, L-F.H. et al. (1993) Science 260:1130.
  2. Milbrandt, J. et al. (1998) Neuron 20:245.
  3. Kotzbauer, P.T. et al. (1996) Nature 384:467.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
  6. Baloh, R.H. et al. (1997) Neuron 18:793.
  7. Jing, S. et al. (1996) Cell 85:1113.
  8. Jing, S. et al. (1997) J Biol Chem 272:33111.
  9. Nishino, J. et al. (1999) Neuron 23:725.
  10. Enomoto, H. et al. (2001) Development 128:3963.
  11. Andres, R. et al. (2001) Development 128:3685.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Artemin Antibody (AF1085)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: Blocking, IHC, IHC-P, In Vivo, Neutralization.

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Showing Publications 1 - 6 of 6.
Publications using AF1085 Applications Species
S Nencini, M Ringuet, DH Kim, C Greenhill, JJ Ivanusic Gdnf, Neurturin and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute To Inflammatory Bone Pain J. Neurosci., 2018-04-30;0(0):. 2018-04-30 [PMID: 29712778] (Neutralization, Rat) Neutralization Rat
Y Han, Q Liu, J Hou, Y Gu, Y Zhang, Z Chen, J Fan, W Zhou, S Qiu, Y Zhang, T Dong, N Li, Z Jiang, H Zhu, Q Zhang, Y Ma, L Zhang, Q Wang, Y Yu, N Li, X Cao Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression Cell, 2018-03-29;0(0):. 2018-03-29 [PMID: 29606356] (In Vivo, Neutralization, Mouse) In Vivo, Neutralization Mouse
The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin Nat. Immunol., 2016-11-21;0(0):. 2016-11-21 [PMID: 27869817] (IHC-P, Mouse) IHC-P Mouse
Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence Nature, 2016-07-13;535(7612):440-3. 2016-07-13 [PMID: 27409807] (Blocking, Mouse) Blocking Mouse
Li J, Klein C, Liang C, Rauch R, Kawamura K, Hsueh AJ Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice. FEBS Lett., 2009-07-04;583(15):2479-85. 2009-07-04 [PMID: 19580811] (Neutralization, Mouse) Neutralization Mouse
Elitt CM, McIlwrath SL, Lawson JJ, Malin SA, Molliver DC, Cornuet PK, Koerber HR, Davis BM, Albers KM Artemin overexpression in skin enhances expression of TRPV1 and TRPA1 in cutaneous sensory neurons and leads to behavioral sensitivity to heat and cold. J. Neurosci., 2006-08-16;26(33):8578-87. 2006-08-16 [PMID: 16914684] (IHC, Mouse) IHC Mouse

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Gene Symbol ARTN