HSP70/HSPA1A Antibody

Images

 
Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.
Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Simple Western: HSP70/HSPA1A Antibody [NBP1-77456] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, EM, IHC, IHC-P, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.01 mg/ml

HSP70/HSPA1A Antibody Summary

Background
Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.
Immunogen
A synthetic peptide made to a C-terminus portion of the human Hsp70 protein (between residues 600-641) [UniProt # P08107]
Localization
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:50
  • Electron Microscopy
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Block/Neutralize
Application Notes
Use in EM reported in scientific literature (PMID:33335563). This Hsp70 antibody is useful for Western blot and IHC-paraffin embedded sections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Block/Neutralize reported in scientific literature (PMID:30417859).
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Human Testis Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NBP1-77456 in the following applications:

  • B/N
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.01 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP70/HSPA1A Antibody

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP70/HSPA1A Antibody (NBP1-77456)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: B/N.


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B/N
(1)
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Product General Protocols

View specific protocols for HSP70/HSPA1A Antibody (NBP1-77456): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77456AF350
Alexa Fluor 405 NBP1-77456AF405
Alexa Fluor 488 NBP1-77456AF488
Alexa Fluor 532 NBP1-77456AF532
Alexa Fluor 594 NBP1-77456AF594
Alexa Fluor 647 NBP1-77456AF647
Alexa Fluor 700 NBP1-77456AF700
Alexa Fluor 750 NBP1-77456AF750
Biotin NBP1-77456B
DyLight 350 NBP1-77456UV
DyLight 405 NBP1-77456V
DyLight 488 NBP1-77456G
DyLight 550 NBP1-77456R
DyLight 594 NBP1-77456DL594
DyLight 650 NBP1-77456C
DyLight 680 NBP1-77456FR
DyLight 755 NBP1-77456IR
FITC NBP1-77456F
HRP NBP1-77456H
Janelia Fluor 549 NBP1-77456JF549
Janelia Fluor 646 NBP1-77456JF646

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Bioinformatics

Gene Symbol HSPA1A
Uniprot