Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.
Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Simple Western: HSP70/HSPA1A Antibody [NBP1-77456] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.
A synthetic peptide made to a C-terminus portion of the human Hsp70 protein (between residues 600-641) [UniProt # P08107]
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Immunogen affinity purified
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Use in EM reported in scientific literature (PMID:33335563). This Hsp70 antibody is useful for Western blot and IHC-paraffin embedded sections.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Block/Neutralize reported in scientific literature (PMID:30417859).
Aoki S, Harada K, Kawai S, et al. Expression of Heat Shock Proteins in Response to Mild Short-term Heat Shock in Human Deciduous Dental Pulp Fibroblast-like Cells Journal of Hard Tissue Biology Jan 26 2021
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