NIK/MAP3K14 Antibody

Images

 
Western Blot: NIK/MAP3K14 Antibody [NBP2-23603] - Detection of MAP3K14 in U2OS cell lysate.
Immunocytochemistry/ Immunofluorescence: NIK/MAP3K14 Antibody [NBP2-23603] - MAP3K14 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry: NIK/MAP3K14 Antibody [NBP2-23603] - Analysis of MAP3K14 in mouse small intestine.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.0 mg/ml

NIK/MAP3K14 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human MAP3K14 protein (between residues 800-900) [UniProt Q99558]
Localization
Cytoplasmic, nuclear and vesicular.
Predicted Species
Rat (92%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This MAP3K14 antibody detects a band at ~104 kDa in Western blot. In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in U2OS cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse. Predicted to react with monkey and rat based on sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NIK/MAP3K14 Antibody

  • EC 2.7.11.25
  • FTDCR1B
  • HS
  • HSNIK
  • HSNIKserine/threonine protein-kinase
  • MAP3K14
  • mitogen-activated protein kinase kinase kinase 14
  • NIK
  • NIKNF-kappa-beta-inducing kinase
  • Serine/threonine-protein kinase NIK

Background

MAP3K14, or NIK (Nuclear Factor-KB Inducing Kinase) is a serine/threonine protein kinase that activates the canonical and non-canonical NF-KB pathways to control transcription of proteins such as cytokines, chemokines and NF-KB signaling molecules. The NK-KB family of transcription factors is composed of a number of structurally related proteins that modulate an assortment of physiological processes from immune responses to cell death and survival. Regulation of NK-KB occurs by two distinct pathways; the canonical pathway mediated by the inhibitor of KB kinase complex, and the non-canonical pathway, involving p100 processing. In the canonical pathway NIK interacts and activates IKK alpha and IKK beta resulting in the release of RelA and p50 for gene transcription. In the non-canonical pathway, MAP3K14 (NIK) phosphorylates p100, resulting in its partial proteolysis to p52, which in turn mediates gene expression (PMID:20685151).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NIK/MAP3K14 Antibody (NBP2-23603) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-23603AF350
Alexa Fluor 405 NBP2-23603AF405
Alexa Fluor 488 NBP2-23603AF488
Alexa Fluor 532 NBP2-23603AF532
Alexa Fluor 594 NBP2-23603AF594
Alexa Fluor 647 NBP2-23603AF647
Alexa Fluor 700 NBP2-23603AF700
Alexa Fluor 750 NBP2-23603AF750
Biotin NBP2-23603B
DyLight 350 NBP2-23603UV
DyLight 405 NBP2-23603V
DyLight 488 NBP2-23603G
DyLight 550 NBP2-23603R
DyLight 594 NBP2-23603DL594
DyLight 650 NBP2-23603C
DyLight 680 NBP2-23603FR
DyLight 755 NBP2-23603IR
HRP NBP2-23603H
Janelia Fluor 549 NBP2-23603JF549
Janelia Fluor 646 NBP2-23603JF646

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Bioinformatics Tool for NIK/MAP3K14 Antibody (NBP2-23603)

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Bioinformatics

Gene Symbol MAP3K14
Entrez
Uniprot