NIK/MAP3K14 Antibody - BSA Free

Western Blot: NIK/MAP3K14 Antibody [NBP2-23603] - Detection of MAP3K14 in U2OS cell lysate.

Immunocytochemistry/Immunofluorescence: NIK/MAP3K14 Antibody [NBP2-23603] - MAP3K14 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: NIK/MAP3K14 Antibody [NBP2-23603] - Analysis of MAP3K14 in mouse small intestine.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.0 mg/ml

NIK/MAP3K14 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human MAP3K14 protein (between residues 800-900) [UniProt Q99558]
• Localization: Cytoplasmic, nuclear and vesicular.
• Predicted Species: Rat (92%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 2.0 ug/ml
• Application Notes: This MAP3K14 antibody detects a band at ~104 kDa in Western blot. In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in U2OS cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Predicted to react with monkey and rat based on sequence homology.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NIK/MAP3K14 Antibody - BSA Free

• EC 2.7.11.25
• FTDCR1B
• HS
• HSNIK
• HSNIKserine/threonine protein-kinase
• MAP3K14
• mitogen-activated protein kinase kinase kinase 14
• NIK
• NIKNF-kappa-beta-inducing kinase
• Serine/threonine-protein kinase NIK

Background

MAP3K14, or NIK (Nuclear Factor-KB Inducing Kinase) is a serine/threonine protein kinase that activates the canonical and non-canonical NF-KB pathways to control transcription of proteins such as cytokines, chemokines and NF-KB signaling molecules. The NK-KB family of transcription factors is composed of a number of structurally related proteins that modulate an assortment of physiological processes from immune responses to cell death and survival. Regulation of NK-KB occurs by two distinct pathways; the canonical pathway mediated by the inhibitor of KB kinase complex, and the non-canonical pathway, involving p100 processing. In the canonical pathway NIK interacts and activates IKK alpha and IKK beta resulting in the release of RelA and p50 for gene transcription. In the non-canonical pathway, MAP3K14 (NIK) phosphorylates p100, resulting in its partial proteolysis to p52, which in turn mediates gene expression (PMID:20685151).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NIK/MAP3K14 Antibody (NBP2-23603)(1)

Publication using NBP2-23603

• Shiode Y, Kodama T, Shigeno S et al. TNF receptor-related factor 3 inactivation promotes the development of intrahepatic cholangiocarcinoma through NF-kappa B-inducing kinase-mediated hepatocyte transdifferentiation Hepatology (Baltimore, Md.) 2022-01-07 [PMID: 34995376] (IHC-P)

Bioinformatics

• Gene Symbol: MAP3K14
• Entrez:
  • 9020(Human)
  • 53859(Mouse)
• Uniprot:
  • Q99558()
  • Q9WUL6(Mouse)