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NIK/MAP3K14 Antibody - BSA Free

Images

 
Western Blot: NIK/MAP3K14 Antibody [NBP2-23603] - Detection of MAP3K14 in U2OS cell lysate.
Immunocytochemistry/ Immunofluorescence: NIK/MAP3K14 Antibody [NBP2-23603] - MAP3K14 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry: NIK/MAP3K14 Antibody [NBP2-23603] - Analysis of MAP3K14 in mouse small intestine.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
2.0 mg/ml

Order Details

NIK/MAP3K14 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human MAP3K14 protein (between residues 800-900) [UniProt Q99558]
Localization
Cytoplasmic, nuclear and vesicular.
Predicted Species
Rat (92%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 2.0 ug/ml
Application Notes
This MAP3K14 antibody detects a band at ~104 kDa in Western blot. In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in U2OS cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Publications
Read Publication using NBP2-23603.

Reactivity Notes

Human and mouse. Predicted to react with monkey and rat based on sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NIK/MAP3K14 Antibody - BSA Free

  • EC 2.7.11.25
  • FTDCR1B
  • HS
  • HSNIK
  • HSNIKserine/threonine protein-kinase
  • MAP3K14
  • mitogen-activated protein kinase kinase kinase 14
  • NIK
  • NIKNF-kappa-beta-inducing kinase
  • Serine/threonine-protein kinase NIK

Background

MAP3K14, or NIK (Nuclear Factor-KB Inducing Kinase) is a serine/threonine protein kinase that activates the canonical and non-canonical NF-KB pathways to control transcription of proteins such as cytokines, chemokines and NF-KB signaling molecules. The NK-KB family of transcription factors is composed of a number of structurally related proteins that modulate an assortment of physiological processes from immune responses to cell death and survival. Regulation of NK-KB occurs by two distinct pathways; the canonical pathway mediated by the inhibitor of KB kinase complex, and the non-canonical pathway, involving p100 processing. In the canonical pathway NIK interacts and activates IKK alpha and IKK beta resulting in the release of RelA and p50 for gene transcription. In the non-canonical pathway, MAP3K14 (NIK) phosphorylates p100, resulting in its partial proteolysis to p52, which in turn mediates gene expression (PMID:20685151).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NIK/MAP3K14 Antibody (NBP2-23603)(1)

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NIK/MAP3K14 Antibody (NBP2-23603) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for NIK/MAP3K14 Antibody (NBP2-23603)

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Bioinformatics

Gene Symbol MAP3K14
Entrez
Uniprot