GRP78/HSPA5 Antibody - BSA Free

Images

 
Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Biological Strategies: Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - C. elegans granulins impair organismal fitness and resistance to ER stress. Total worm lysates from synchronized day 1 adult ...read more
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis of MAM-associated protein levels in control and patients' fibroblasts.(A) Equal amounts from one control and the five patients were loaded (50 ug of total cell ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/mL overnight ...read more
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on NIH3T3 cells with GRP78/HSPA5 Antibody NBP1-06274AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Detection of Bip/Grp78 on HeLa whole cell extracts using NBP1-06274.
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis using Alexa Fluor (R) 488 conjugate of NBP1-06274. An intracellular stain was performed on HeLa cells with GPR78/HSPA5 antibody NBP1-06274 (blue) and a ...read more
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on Jurkat cells with Alexa Fluor 647 conjugate of GPR78/HSPA5 antibody NBP1-06274AF647 (blue) and a matched isotype control ...read more
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, Ch, Dr, Sh, Ze, Bv, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IHC-FrFl
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

GRP78/HSPA5 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]
Localization
Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Marker
ER Stress Marker
Predicted Species
Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:150
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western 1:25
  • Western Blot 0.5 ug/ml
Application Notes
In Western blot a band is seen at ~78 kDa. For information on the flow cytometry application, see the publication PMID: 20208072. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24089213). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 24089213). Use in Immunohistochemistry free floating reported in scientific literature (PMID: 26329458).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Control Peptide
GRP78/HSPA5 Peptide (NBP1-06274PEP)
Publications
Read Publications using
NBP1-06274 in the following applications:

Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:34327238) C. elegans reactivity reported in scientific literature (PMID: 31398187).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GRP78/HSPA5 Antibody - BSA Free

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP78
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2

Background

HSP70 family member GRP78 (78 kD glucose-regulated protein) is localized in ER lumen as well as melanosomes (stage I to stage IV) where it facilitates protein folding/assembly, protein quality control, Ca2+ binding, and regulates ER stress signaling. GRP78 interacts with DNAJC1 and is a component of EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) as well as chaperone multiprotein complex (DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1, ERP29). GRP78 also interacts with TMEM132A as well as TRIM21, and may form a complex with ERLEC1, OS9, SEL1L and SYVN1. In cancerous cells, GRP78 overexpression is associated with UPR activation, including upregulation of associated proteins such as PERK, ATF6, CHOP etc that further regulate JNK and NF-kB untimately leading to survival, tumor progression, angiogenesis, resistance to therapy and metastasis. GRP78 is a signaling receptor for activated alpha2-macroglobulin, plasminogen kringle 5, and microplasminogen, and plays role in viral entry of coxsackie B as well as dengue fever viruses. GRP78 is also involved in regulation of tissue factor procoagulant activity and functions as a receptor for angiogenic peptides via VEGFRs independent mechanism. Cell surface GRP78 is found associated with diverse proteins including VDAC, MHC-I, Cripto and DnaJ-like protein MTJ-1. Because GRP78 is present on the surface of cancer cells but not on normal cells in vivo, it provide an exciting opportunity for cancer targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-1335
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
NB300-619
Species: Bv, Ch, Ha, Hu, Mu, Rt
Applications: ICC, ICC/IF, IHC, IHC-P, IP, WB
NBP1-40256
Species: Hu, Mu, Pl, Po, Rb, Rt
Applications: ChIP, CyTOF-ready, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB
NBP1-77681
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
NBP2-67817
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB600-101
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB
NBP1-80930
Species: Ch, Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56708
Species: Ch, ChHa, Hu, Ma, Mu, Po, Rt
Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
NB100-1965
Species: Av, Bv, Ch, Dr, Gp, Hu, Mu, Po, Rb, Rt, Sh, Xp, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP1-76801
Species: Ch, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB100-2323
Species: Dr, Gt, SyHa, Hu, Ma, Pm, Mu, Po, Pm, Rb, Rt
Applications: ChIP, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, WB
NB300-517
Species: Bv, Ha, Hu, Pm, Mu, Po, Pm, Rb, Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, KD, WB
NBP1-89394
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-37761
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-1921
Species: Bv, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
H00000468-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-67514
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP1-06274
Species: Hu, Mu, Rt, Ce, Ch, Dr, Sh, Ze, Bv, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IHC-FrFl

Publications for GRP78/HSPA5 Antibody (NBP1-06274)(33)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, C. elegans, Drosophila.

We have publications tested in 7 applications: FLOW, Flow, ICC/IF, IHC, IHC-FrFl, IHC-P, WB.


Filter By Application
FLOW
(2)
Flow
(1)
ICC/IF
(3)
IHC
(1)
IHC-FrFl
(1)
IHC-P
(1)
WB
(21)
All Applications
Filter By Species
Human
(6)
Mouse
(10)
Rat
(1)
C. elegans
(1)
Drosophila
(2)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NBP1-06274 Applications Species
Rossin F, Avitabile E, Catarinella G et al. Reticulon-1C Involvement in Muscle Regeneration Metabolites Dec 8 2021 [PMID: 34940613]
Scapin C, Ferri C, Pettinato E et al. Enhanced axonal Neuregulin-1 type-III signaling ameliorates disease severity in a CMT1B mouse model bioRxiv Aug 9 2018 (WB, Mouse) WB Mouse
Stojkovska I, Wani WY, Zunke F Et al. Rescue of alpha-synuclein aggregation in Parkinson's patient neurons by synergistic enhancement of ER proteostasis and protein trafficking Neuron Nov 9 2021 [PMID: 34793693]
Arquier N, Bjordal M, Hammann P et al. Brain adiponectin signaling controls peripheral insulin response in Drosophila Nature communications Sep 24 2021 [PMID: 34561451] (WB, Drosophila) WB Drosophila
VerPlank JJS, Gawron J, Silvestri NJ Et al. Raising cGMP restores proteasome function and myelination in mice with a proteotoxic neuropathy Brain : a journal of neurology Aug 11 2021 [PMID: 34382059] (WB) WB
Li Lp, Zhong J, Li Mh Et Al. Disruption of MAP7D1 Gene Function Increases the Risk of Doxorubicin-Induced Cardiomyopathy and Heart Failure BioMed research international Jul 15 2021 [PMID: 34327238] (WB) WB
Nam YW, Kong D, Wang D, et al. Differential modulation of SK channel subtypes by phosphorylation Cell calcium Jan 6 2021 [PMID: 33422768] (WB, Mouse) WB Mouse
Asahina, M, Fujinawa, R Et al. Ngly1 -/- rats develop neurodegenerative phenotypes and pathological abnormalities in their peripheral and central nervous systems. Hum Mol Genet Jun 27 2020 [PMID: 32259258] (Flow, Human) Flow Human
Chen X, Li Z, Lv Y et al. Proteomic Analysis of Capacitated and Non-Capacitated Spermatozoa of Yanbian Yellow Cattle Research Square Dec 15 2020
Arioka Y, Shishido E, Kushima I et al. Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons EBioMedicine Dec 17 2020 [PMID: 33341442] (WB, Human) WB Human
Show All 33 Publications.

Reviews for GRP78/HSPA5 Antibody (NBP1-06274) (0)

There are no reviews for GRP78/HSPA5 Antibody (NBP1-06274). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for GRP78/HSPA5 Antibody (NBP1-06274): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRP78/HSPA5 Antibody (NBP1-06274). (Showing 1 - 1 of 1 FAQs).

  1. I have received the Novus Cat # for a human GRP78-specific rabbit Ab from a lab that had used it and achieved some nice results with it. They suggest we use the same Ab to reproduce their data. However, I have not been able to find a product with that Cat # in your online search engine. Perhaps the product has been discontinued, or was given a different Cat #? Could you please look into this? The Ab they used was: GRP78 Cat # NBP1-49339, Lot # A113911. In case the product is no longer available, could you please recommend something that should be equivalent or very similar to it?
    • NBP1-49339 has been discontinued but NBP1-06274 uses a similar antigen that falls between amino acids 250 and 300 and works in Western blot and ICC. ICC and flow use essentially the same techniques, so it should work just fine if you were using it in flow or Western blot before.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional GRP78/HSPA5 Products

Bioinformatics Tool for GRP78/HSPA5 Antibody (NBP1-06274)

Discover related pathways, diseases and genes to GRP78/HSPA5 Antibody (NBP1-06274). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GRP78/HSPA5 Antibody (NBP1-06274)

Discover more about diseases related to GRP78/HSPA5 Antibody (NBP1-06274).
 

Pathways for GRP78/HSPA5 Antibody (NBP1-06274)

View related products by pathway.

PTMs for GRP78/HSPA5 Antibody (NBP1-06274)

Learn more about PTMs related to GRP78/HSPA5 Antibody (NBP1-06274).
 

Research Areas for GRP78/HSPA5 Antibody (NBP1-06274)

Find related products by research area.

Blogs on GRP78/HSPA5.

GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein ...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GRP78/HSPA5 Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol HSPA5