GRP78/HSPA5 Antibody

Images

 
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 5 ug/ml overnight at ...read more
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with GRP78/HSPA5 Antibody NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized ...read more
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Detection of Bip/Grp78 on HeLa whole cell extracts using NBP1-06274.
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis using Alexa Fluor (R) 488 conjugate of NBP1-06274. An intracellular stain was performed on HeLa cells with GPR78/HSPA5 antibody NBP1-06274 (blue) and a ...read more
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Jurkat cells with GPR78/HSPA5 antibody NBP1-06274AF647 (blue) and a matched isotype ...read more
Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Dr, Sh, Bv, Ce, Pm, ZeSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

GRP78/HSPA5 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]
Localization
Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Marker
ER Stress Marker
Predicted Species
Primate (100%), C. elegans (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Simple Western 1:25
  • Flow Cytometry 1:150
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This GRP78 antibody is useful for immunocytochemistry/immunofluorescence and western blot where a band is seen at approx. 78 kDa. For information on the flow cytometry application, see the publication PMID: 20208072. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24089213)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Control Peptide
GRP78/HSPA5 Peptide (NBP1-06274PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-06274 in the following applications:

Publications
Read Publications using
NBP1-06274 in the following applications:

Reactivity Notes

Human, mouse, rat, sheep and chicken. Predicted to react with Zebrafish, C. elegans, primate, bovine based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GRP78/HSPA5 Antibody

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP78
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2

Background

HSP70 family member GRP78 (78 kD glucose-regulated protein) is localized in ER lumen as well as melanosomes (stage I to stage IV) where it facilitates protein folding/assembly, protein quality control, Ca2+ binding, and regulates ER stress signaling. GRP78 interacts with DNAJC1 and is a component of EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) as well as chaperone multiprotein complex (DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1, ERP29). GRP78 also interacts with TMEM132A as well as TRIM21, and may form a complex with ERLEC1, OS9, SEL1L and SYVN1. In cancerous cells, GRP78 overexpression is associated with UPR activation, including upregulation of associated proteins such as PERK, ATF6, CHOP etc that further regulate JNK and NF-kB untimately leading to survival, tumor progression, angiogenesis, resistance to therapy and metastasis. GRP78 is a signaling receptor for activated alpha2-macroglobulin, plasminogen kringle 5, and microplasminogen, and plays role in viral entry of coxsackie B as well as dengue fever viruses. GRP78 is also involved in regulation of tissue factor procoagulant activity and functions as a receptor for angiogenic peptides via VEGFRs independent mechanism. Cell surface GRP78 is found associated with diverse proteins including VDAC, MHC-I, Cripto and DnaJ-like protein MTJ-1. Because GRP78 is present on the surface of cancer cells but not on normal cells in vivo, it provide an exciting opportunity for cancer targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-06274
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Applications: WB, Simple Western, Flow, ICC/IF

Publications for GRP78/HSPA5 Antibody (NBP1-06274)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC-FrFl, IHC-P, WB.


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FLOW
(1)
ICC/IF
(3)
IHC-FrFl
(1)
IHC-P
(1)
WB
(9)
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Human
(3)
Mouse
(7)
Rat
(1)
Drosophila
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP1-06274 Applications Species
Ren Z, Wang X, Yang F et al. Ethanol-induced Damage to the Developing Spinal Cord: The Involvement of CCR2 Signaling Biochim. Biophys. Acta 2017 Aug 01 [PMID: 28778590] (WB, Mouse) WB Mouse
Liu YQ, Jia MQ, Xie ZH et al. Arrestins contribute to amyloid beta-induced cell death via modulation of autophagy and the alpha7nAch receptor in SH-SY5Y cells. Sci Rep. 2017 Jun 13 [PMID: 28611418] (WB, Human) WB Human
Kunde DA, Chong WC, Nerurkar PV et al. Bitter melon protects against ER stress in LS174T colonic epithelial cells BMC Complement Altern Med Jan 3 2017 [PMID: 28049460] (WB) WB
Sidoli M, Musner N, Silvestri N et al. Ablation of Perk in Schwann Cells Improves Myelination in the S63del Charcot-Marie-Tooth 1B Mouse. J. Neurosci. Nov 2 2016 [PMID: 27807175] (WB, Mouse) WB Mouse
Ren Z, Yang F, Wang X et al. Chronic plus binge ethanol exposure causes more severe pancreatic injury and inflammation. Toxicol. Appl. Pharmacol. Aug 15 2016 [PMID: 27538709] (WB, Mouse) WB Mouse
Ren Z, Wang X, Xu M et al. Binge ethanol exposure causes endoplasmic reticulum stress, oxidative stress and tissue injury in the pancreas. Oncotarget. Aug 23 2016 [PMID: 27527870] (ICC/IF, Rat) ICC/IF Rat
Niccoli T, Cabecinha M, Tillmann A et al. Increased Glucose Transport into Neurons Rescues Abeta Toxicity in Drosophila. Curr. Biol. Aug 9 2016 [PMID: 27524482] (WB, Drosophila) WB Drosophila
Rasche L, Menoret E, Dubljevic V et al. A GRP78-directed monoclonal antibody recaptures response in refractory multiple myeloma with extramedullary involvement. Clin. Cancer Res. 2016 Mar 30 [PMID: 27029491] (FLOW, Human)

Details:
This publication used the DL488 conjugated form of this antibody (NBP1-06274G)
FLOW Human
Martinez-Pizarro A, Desviat LR, Ugarte M et al. Endoplasmic Reticulum Stress and Autophagy in Homocystinuria Patients with Remethylation Defects. PLoS ONE. 2016 Mar 10 [PMID: 26959487] (WB, Human) WB Human
Henry FE, Sugino K, Tozer A et al. Cell type-specific transcriptomics of hypothalamic energy-sensing neuron responses to weight-loss. Elife 2015 Sep 02 [PMID: 26329458] (IHC-FrFl, Mouse)

Details:
GRP78 antibody was used at 1:4000 dilution for IHC- free floating sections on brains of Agrp(Cre), Agrptm1(cre)Lowl/J), Ai9 (ROSA-loxPStoploxP-tdTomato), Pomc(topazFP), and Npy(hrGFP) mice.
IHC-FrFl Mouse
Show All 15 Publications.

Review for GRP78/HSPA5 Antibody (NBP1-06274) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-06274:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot GRP78/HSPA5 NBP1-06274
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reviewed by:
Anonymous
WB Other 07/27/2016
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Summary

ApplicationWestern Blot
Sample TestedWhole embryos lysate
SpeciesOther

Blocking

Blocking Details4% non-fat milk

Primary Anitbody

Dilution Ratio1:2000, overnight, at 4 degree

Secondary Antibody

Secondary Descriptionanti-rabbit IgG HRP

Details

Detection NotesX-ray film, exposed for 1 minute

Product General Protocols

View specific protocols for GRP78/HSPA5 Antibody (NBP1-06274): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRP78/HSPA5 Antibody (NBP1-06274). (Showing 1 - 1 of 1 FAQs).

  1. I have received the Novus Cat # for a human GRP78-specific rabbit Ab from a lab that had used it and achieved some nice results with it. They suggest we use the same Ab to reproduce their data. However, I have not been able to find a product with that Cat # in your online search engine. Perhaps the product has been discontinued, or was given a different Cat #? Could you please look into this? The Ab they used was: GRP78 Cat # NBP1-49339, Lot # A113911. In case the product is no longer available, could you please recommend something that should be equivalent or very similar to it?
    • NBP1-49339 has been discontinued but NBP1-06274 uses a similar antigen that falls between amino acids 250 and 300 and works in Western blot and ICC. ICC and flow use essentially the same techniques, so it should work just fine if you were using it in flow or Western blot before.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-06274AF405
Alexa Fluor 488 Labeled NBP1-06274AF488
Alexa Fluor 647 Labeled NBP1-06274AF647
Alexa Fluor 700 Labeled NBP1-06274AF700
Biotin Labeled NBP1-06274B
DyLight 405 Labeled NBP1-06274V
DyLight 488 Labeled NBP1-06274G
DyLight 550 Labeled NBP1-06274R
DyLight 650 Labeled NBP1-06274C
FITC Labeled NBP1-06274F
HRP Labeled NBP1-06274H

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Bioinformatics Tool for GRP78/HSPA5 Antibody (NBP1-06274)

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PTMs for GRP78/HSPA5 Antibody (NBP1-06274)

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Blogs on GRP78/HSPA5.

GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein...  Read full blog post.

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Recent Reviews

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1
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Anonymous
07/27/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol HSPA5
Entrez