Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/ml overnight ...read more
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with GRP78/HSPA5 Antibody NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized ...read more
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Detection of Bip/Grp78 on HeLa whole cell extracts using NBP1-06274.
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 5 ug/ml overnight at ...read more
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis using Alexa Fluor (R) 488 conjugate of NBP1-06274. An intracellular stain was performed on HeLa cells with GPR78/HSPA5 antibody NBP1-06274 (blue) and a ...read more
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Jurkat cells with GPR78/HSPA5 antibody NBP1-06274AF647 (blue) and a matched isotype ...read more
Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
This GRP78 antibody is useful for immunocytochemistry/immunofluorescence and western blot where a band is seen at approx. 78 kDa. For information on the flow cytometry application, see the publication PMID: 20208072. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24089213)
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
78 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, mouse, rat, sheep and chicken. Predicted to react with Zebrafish, C. elegans, primate, bovine based on 100% sequence homology.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for GRP78/HSPA5 Antibody
78 kDa glucose-regulated protein
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
Heat shock 70 kDa protein 5
heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Immunoglobulin heavy chain-binding protein
HSP70 family member GRP78 (78 kD glucose-regulated protein) is localized in ER lumen as well as melanosomes (stage I to stage IV) where it facilitates protein folding/assembly, protein quality control, Ca2+ binding, and regulates ER stress signaling. GRP78 interacts with DNAJC1 and is a component of EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) as well as chaperone multiprotein complex (DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1, ERP29). GRP78 also interacts with TMEM132A as well as TRIM21, and may form a complex with ERLEC1, OS9, SEL1L and SYVN1. In cancerous cells, GRP78 overexpression is associated with UPR activation, including upregulation of associated proteins such as PERK, ATF6, CHOP etc that further regulate JNK and NF-kB untimately leading to survival, tumor progression, angiogenesis, resistance to therapy and metastasis. GRP78 is a signaling receptor for activated alpha2-macroglobulin, plasminogen kringle 5, and microplasminogen, and plays role in viral entry of coxsackie B as well as dengue fever viruses. GRP78 is also involved in regulation of tissue factor procoagulant activity and functions as a receptor for angiogenic peptides via VEGFRs independent mechanism. Cell surface GRP78 is found associated with diverse proteins including VDAC, MHC-I, Cripto and DnaJ-like protein MTJ-1. Because GRP78 is present on the surface of cancer cells but not on normal cells in vivo, it provide an exciting opportunity for cancer targeting.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GRP78/HSPA5 Antibody (NBP1-06274). (Showing 1 - 1 of 1 FAQs).
I have received the Novus Cat # for a human GRP78-specific rabbit Ab from a lab that had used it and achieved some nice results with it. They suggest we use the same Ab to reproduce their data. However, I have not been able to find a product with that Cat # in your online search engine. Perhaps the product has been discontinued, or was given a different Cat #? Could you please look into this? The Ab they used was: GRP78 Cat # NBP1-49339, Lot # A113911. In case the product is no longer available, could you please recommend something that should be equivalent or very similar to it?
NBP1-49339 has been discontinued but NBP1-06274 uses a similar antigen that falls between amino acids 250 and 300 and works in Western blot and ICC. ICC and flow use essentially the same techniques, so it should work just fine if you were using it in flow or Western blot before.
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