Human Liver Whole Tissue Lysate (Adult Whole Normal)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Liver Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Liver

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot 10 ug - 20 ug
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Publications
Read Publications using
NB820-59232 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Alternate Names for Human Liver Whole Tissue Lysate (Adult Whole Normal)

  • Liver lysate
  • liver lysates
  • Liver

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Liver Lysate (NB820-59232)(6)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Liver Lysate (NB820-59232). (Showing 1 - 2 of 2 FAQs).

  1. Which fraction is contained in this lysate? Also, on the datasheet, the applications of this lysate include enzyme activity analysis - what does this mean? Lastly, could you tell me if using RIPA buffer affects the enzyme activity or not?
    • The adult normal human liver whole tissue lysate with catalog number NB820-59232 is prepared from whole tissue homogenates and is stored in HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent with a cocktail of protease inhibitors. As such, the activity of the expressed enzymes should be preserved. During the Quality Control process the protein lysate is verified on SDS-PAGE analysis as a consistent pattern comparing to a standard protein lysate. This uses the following biomarkers: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein. NB820-59232 is validated for Western blotting and immunoprecipitation, and is guaranteed for 6 months from the date of receipt. We recommend that you store this product at -80C and avoid freeze-thaw cycles.
  2. Regarding Human Liver Whole Tissue Lysate (Adult Whole Normal) NB820-59232 and Human Liver Whole Tissue Lysate (Adult Whole Liver Cirrhosis):1. Does the lysate also contain the membrane protein? If yes, will it be possible to isolate the membrane fraction from the lysate? For my experiment I segregate the cytosolic and membrane fraction.2. Do you treat , Human Liver Whole Tissue Lysate (Adult Whole Liver Cirrhosis) NB820-59328, with DNase1 to avoid coagulation while heating prior to loading the lysate in SDS running gel? If not DNase1, what you use to avoid the coagulation?
    • All Whole Tissue Lysates are prepared using the following kit:https://www.novusbio.com/products/total-protein-kit_nbp2-37853We don’t treat the lysates with DNase. We do not notice any coagulation when loading on an SDS-PAGE gel. Just heating the sample in standard loading buffer works well. The whole tissue lysates should contain plasma membrane fragments, however some components (e.g. mitochondria, lysosomes) will probably precipitate in the final 18,000g spin.We do however have corresponding membrane fractions:Normal: https://www.novusbio.com/products/liver-lysate_nb820-60598Normal, left lobe: https://www.novusbio.com/products/liver-lysate_nb820-60599Cirrhosis: https://www.novusbio.com/products/liver-lysate_nbl1-26449

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