Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Skeletal Muscle

Applications/Dilutions

Dilutions
  • Western Blot 10 ug - 20 ug
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Publications
Read Publications using NB820-59253.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Skeletal Muscle

Type
Tissue
Life Stage
Adult
Tissue
Skeletal Muscle
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Skeletal Muscle Lysate (NB820-59253)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Skeletal Muscle Lysate (NB820-59253). (Showing 1 - 2 of 2 FAQs).

  1. I recently ordered whole normal skeletal muscle lysate and whole normal liver lysate (NB820-59253 and 59232). What are the exact concentrations for the buffer components that each of the lysates is in? I need to dilute the lysate because the concentration given is too high (5mg/mL), but I want to dilute it in whatever buffer it is in. Can you recommend what I should do?
    • Unfortunately the concentrations of the components (stated on the datasheet) are proprietary. I apologize. If the concentration is too high, our lab recommends simply diluting it in your loading buffer. A 4x or higher loading buffer should suffice.
  2. I am interested in your skeletal muscle total protein, including NB820-59294, NB820-59253 and NBL1-26511. I would like to know if you have the skeletal muscle tissue from different individuals? Are all of they from the same people? How detailed information about the donor could you offer us? Could you give us the body weight, height, blood glucose of the donor? Do you know the diabetic donor is type 1 or type 2 diabetes? Do you have any scientific papers using this product?
    • NB820-59294 comes from a 67 year old male donor. NB820-59253 comes from a 24 year old male donor. NBL1-26511 comes from a 79 year old male donor. This patient had type II diabetes. That is the only other information I have on the patient at this time. I also have no publications using this product at the moment.

Additional Skeletal Muscle Products

Skeletal Muscle NB820-59253

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