HSP27 Antibody (G3.1)

Images

 
Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).
Immunocytochemistry/ Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear ...read more
Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffin-embedded human Breast Carcinome stained with HSP27 Monoclonal Antibody (G3.1)
Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffim-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Pm, Pm, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
G3.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HSP27 Antibody (G3.1) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34621)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Localization
Predominantly cytoplasmic with some nuclear
Specificity
monoclonal antibody G3.1 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HSPB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-32972 in the following applications:

Publications
Read Publications using
NBP2-32972 in the following applications:

  • B/N
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for HSP27 Antibody (G3.1)

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP27 Antibody (NBP2-32972)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: B/N, WB.


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B/N
(1)
WB
(3)
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Mouse
(1)
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Showing Publications 1 - 4 of 4.
Publications using NBP2-32972 Applications Species
Schwab M, Thunborg K, Azimzadeh O et al. Abstract 5426: Down-regulation of cancer metabolism enhances radiosensitivity by impairing the heat shock response Cancer Research 2022-06-15
Schwab M, Dezfouli A, Khosravi M et al. The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response Research Square 2023-03-20 (WB) WB
Schwab M, Multhoff G A Low Membrane Hsp70 Expression in Tumor Cells With Impaired Lactate Metabolism Mediates Radiosensitization by NVP-AUY922 Frontiers in oncology [PMID: 35463341] (WB, Mouse) WB Mouse
Schwab M, Thunborg K, Azimzadeh O et al. Targeting Cancer Metabolism Breaks Radioresistance by Impairing the Stress Response Cancers (Basel) 2021-07-27 [PMID: 34359663] (B/N, WB) B/N, WB

Review for HSP27 Antibody (NBP2-32972) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-32972:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot HSP27 NBP2-32972
Enlarge
4
reviewed by:
Byron Baron
WB Human 12/24/2016
View

Summary

ApplicationWestern Blot
Sample TestedSW480 (colon)
SpeciesHuman
Lot3315-1P150413

Comments

CommentsPrimary mouse anti HSP27 antibody diluted 1:1000 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti mouse antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP27 Antibody (NBP2-32972). (Showing 1 - 1 of 1 FAQs).

  1. What is the concentration of the existing lot of NBP2-32972-0.1ml
    • The concentration for product NBP2-32972 is 0.2mg/ml.

Secondary Antibodies

 

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Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

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Recent Reviews

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4
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1
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Byron Baron
12/24/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSPB1
Uniprot