HSP27 Antibody (G3.1)

Images

 
Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).
Immunocytochemistry/ Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear ...read more
Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffin-embedded human Breast Carcinome stained with HSP27 Monoclonal Antibody (G3.1)
Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffim-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Pm, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
G3.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HSP27 Antibody (G3.1) Summary

Immunogen
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Localization
Predominantly cytoplasmic with some nuclear
Specificity
This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HSPB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HSP27 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP2-32972 in the following applications:

Reactivity Notes

Chimpanzee

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for HSP27 Antibody (G3.1)

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP27 Antibody (NBP2-32972) (0)

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Review for HSP27 Antibody (NBP2-32972) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-32972:
Filter by Applications
WB
(1)
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Images Ratings Applications Species Date Details
Western Blot HSP27 NBP2-32972
Enlarge
4
reviewed by:
Byron Baron
WB Human 12/24/2016
View

Summary

ApplicationWestern Blot
Sample TestedSW480 (colon)
SpeciesHuman
Lot3315-1P150413

Comments

CommentsPrimary mouse anti HSP27 antibody diluted 1:1000 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti mouse antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP27 Antibody (NBP2-32972). (Showing 1 - 1 of 1 FAQ).

  1. What is the concentration of the existing lot of NBP2-32972-0.1ml
    • The concentration for product NBP2-32972 is 0.2mg/ml.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34621AF350
Alexa Fluor 405 NBP2-34621AF405
Alexa Fluor 488 NBP2-34621AF488
Alexa Fluor 532 NBP2-34621AF532
Alexa Fluor 594 NBP2-34621AF594
Alexa Fluor 647 NBP2-34621AF647
Alexa Fluor 700 NBP2-34621AF700
Alexa Fluor 750 NBP2-34621AF750
Allophycocyanin NBP2-34621APC
Biotin NBP2-34621B
DyLight 350 NBP2-34621UV
DyLight 405 NBP2-34621V
DyLight 488 NBP2-34621G
DyLight 550 NBP2-34621R
DyLight 594 NBP2-34621DL594
DyLight 650 NBP2-34621C
DyLight 680 NBP2-34621FR
DyLight 755 NBP2-34621IR
FITC NBP2-34621F
HRP NBP2-34621H
Janelia Fluor 549 NBP2-34621JF549
Janelia Fluor 646 NBP2-34621JF646
PE NBP2-34621PE
PerCP NBP2-34621PCP

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Byron Baron
12/24/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSPB1
Uniprot