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HSF1 Antibody

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Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.
Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ge, Pm, RbSpecies Glossary
Applications WB, EIA, ELISA, GS, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HSF1 Antibody Summary

Immunogen
Recombinant human HSF1.
Specificity
Recognizes nonphosphorylated HSF1 at ~60kDa and phosphorylated HSF1 at ~80kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSF1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 (colorimetric)
  • ELISA
  • Enzyme Immunoassay
  • Gel Super Shift Assays 1:1-1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody is useful in Enzyme Immunoassay, Electrophoretic Mobility Shift Assays, Immunoprecipitation and Western Blot (Detects a band of ~95kDa). ELISA was reported in scientific literature.
Publications
Read Publications using NBP1-97475.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for HSF1 Antibody

  • heat shock factor protein 1
  • heat shock transcription factor 1HSTF1HSF 1
  • HSF1
  • HSTF 1
  • HSTF1

Background

HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: HspA4 Antibody (SD0861) [NBP2-67817] - Staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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HSF1 Antibody
NBP1-97475
HSF1 Antibody
Species: Hu, Mu, Rt, Ge, Pm, Rb
Applications: WB, EIA, ELISA, GS, IP

Publications for HSF1 Antibody (NBP1-97475)(7)

We have publications tested in 4 applications: ELISA, GS, IP, WB.


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Bioinformatics

Gene Symbol HSF1
Entrez
Uniprot