HSF1 Antibody

Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.

• Reactivity: Hu, Mu, Rt, Ma, Pm, Rb
• Applications: WB, ELISA, GS, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HSF1 Antibody Summary

• Immunogen: Recombinant human HSF1.
• Specificity: Recognizes nonphosphorylated HSF1 at ~60kDa and phosphorylated HSF1 at ~80kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Gel Super Shift Assays 1:1-1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000 (colorimetric)
• Application Notes: ELISA reactivity was reported in (PMID:12732732).
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for HSF1 Antibody

• heat shock factor protein 1
• heat shock transcription factor 1HSTF1HSF 1
• HSF1
• HSTF 1
• HSTF1

Background

HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSF1 Antibody (NBP1-97475)(9)

Publications using NBP1-97475

• Onay Ucar E, Sengelen A, Mertoglu Kamali E Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells Biochemical Pharmacology 2023-02-01 [PMID: 36603687] (WB, Human)
  
  Details:
  1:1000 WB dilution
• Kiyga E, Adıguzel Z, Onay Ucar E Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells Molecular biology reports 2022-06-25 [PMID: 35752701] (WB, Human)
• TW Mu et al. Chemical and biological approaches synergize to ameliorate protein-folding diseases. Cell 134, 769 . 2008-01-01 [PMID: 18775310] (WB)
• G Steiner et al. TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response:; . Cell Death Differ 10, 1126. 2003-01-01 [PMID: 14502236] (GS)
• DM Chuang et al. Postinsult treatment with lithium reduces brain damage and facilitates neurological recovery in a rat ischemia/reperfusion model:; . PNAS 100, 6210. 2003-01-01 [PMID: 12732732] (ELISA)
• FR Sharp et al. 17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons. J. Cereb. Blood Flow Metab. 22, 183. 2002-01-01 [PMID: 11823716] (WB)
• M Di Rosa et al. Role of cyclopentenone prostaglandins in rat carrageenin pleurisy:;. FEBS Lett 508, 61. 2001-01-01 [PMID: 11707269] (GS, WB)
• R Voellmy et al. Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex:; J. Biol Chem 276, 45791. 2001-01-01 [PMID: 11583998] (IP)
• DS Latchman et al. Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90 beta gene promoters; J. Biol Chem 274, 1723. 1999-01-01 [PMID: 9880553] (IP)

Bioinformatics

• Gene Symbol: HSF1
• Entrez:
  • 3297(Human)
  • 15499(Mouse)
  • 79245(Rat)
• Uniprot:
  • Q00613(Human)