HSP60 Antibody (NBP1-77397)

Images

 
Western Blot: HSP60 Antibody [NBP1-77397] - Western blot analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was ...read more
Immunohistochemistry: HSP60 Antibody [NBP1-77397] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.
Flow Cytometry: HSP60 Antibody [NBP1-77397] - analysis of HSP60 in RM1 cells (murine prostate cancer cell line) using anti-HSP60 antibody. The primary antibody was used at a dilution of 1:100. Image from verified ...read more
Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody [NBP1-77397] - HSP60 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescence analysis of HeLa cells using Hsp60 antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).
Simple Western: HSP60 Antibody [NBP1-77397] - Simple Western lane view shows a specific band for Hsp60 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, Pm, Bv, ChSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

HSP60 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human Hsp60 protein (within residues 70-150). [Swiss-Prot P10809]
Localization
Mitochondrion matrix
Marker
Mitochondria Marker
Predicted Species
Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Simple Western 1:5000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This Hsp60 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~61 kDa representing Hsp60. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 4 Reviews rated 5
using
NBP1-77397 in the following applications:

Reactivity Notes

Human, mouse, rat, hamster and primate. Immunogen has 100% identity to bovine and chicken.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP60 Antibody

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • GROEL
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • HSP60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13

Background

Heat shock protein 60 activates T cells and ultimately autoimmune actions and diseases. In gastric cancer, HSP60 is upregulated in malignant ascites that have been treated, and induces a tumor-specific cytotoxic T lymphocyte response. Enhancing Hsp60 expression is beneficial for protecting bone tissue against osteoblast apoptosis. Hsp60 is highly influenced by dietary intake of antioxidants, and is a prime target for many natural therapies against gut inflammation, preeclampsia, and some low level bacterial infections. Ischemia and reperfusion, as well as hypoxic environments can cause temporary elevations in Hsp60 expression levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP60 Antibody (NBP1-77397) (0)

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Reviews for HSP60 Antibody (NBP1-77397) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 3 application: Flow, ICC, WB.

Reviews using NBP1-77397:
Filter by Applications
Flow
(1)
ICC
(2)
WB
(1)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry HSP60 NBP1-77397
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reviewed by:
Anonymous
Flow Mouse 12/30/2014
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Summary

ApplicationFlow Cytometry
Sample TestedMurine prostate cancer cell line RM1
SpeciesMouse

Blocking

Blocking DetailsFc blocking antibody for 10' at 4C

Primary Anitbody

Dilution Ratio1:100 in facs buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG-PE
Secondary Concentration1:400

Details

Detection NotesFACS Diva acquisition with a LSRII machine and FlowJo software for analysis
Permeabilization DetailsD Cytofix/Cytoperm™ Kit (Cat. No. 554714) 20' at 4C
Immunocytochemistry HSP60 NBP1-77397
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reviewed by:
Anony- mous
ICC Human 06/05/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsConfocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C). This Hsp60 antibody detected Hsp60 at cytoplasm of HeLa cells in confocal immunofluorescent analysis at dilution of 1:100.
FileView PDF
Western Blot HSP60 NBP1-77397
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reviewed by:
Anony- mous
WB Human 06/05/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa and COS cells using Hsp60 antibody (NBP1-77397, 1:2000). This antibody detected endogenous levels of Hsp60 in HeLa and COS cell lysates in WB at dilution of 1:2000 (0.5 ug/ml).
Immunocytochemistry HSP60 NBP1-77397
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reviewed by:
Anony- mous
ICC Human 05/21/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP60 Antibody (NBP1-77397) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77397AF405
Alexa Fluor 488 Labeled NBP1-77397AF488
Alexa Fluor 647 Labeled NBP1-77397AF647
Alexa Fluor 700 Labeled NBP1-77397AF700
Biotin Labeled NBP1-77397B
DyLight 405 Labeled NBP1-77397V
DyLight 488 Labeled NBP1-77397G
DyLight 550 Labeled NBP1-77397R
DyLight 650 Labeled NBP1-77397C
FITC Labeled NBP1-77397F
HRP Labeled NBP1-77397H

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Blogs on HSP60.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Anonymous
12/30/2014
Application: Flow
Species: Mouse

Anony- mous
06/05/2014
Application: ICC
Species: Human

Anony- mous
06/05/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSPD1
Entrez
Uniprot