HSP60 Antibody


Western Blot: HSP60 Antibody [NBP1-77397] - Western blot analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was ...read more
Immunohistochemistry: HSP60 Antibody [NBP1-77397] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.
Flow Cytometry: HSP60 Antibody [NBP1-77397] - analysis of HSP60 in RM1 cells (murine prostate cancer cell line) using anti-HSP60 antibody. The primary antibody was used at a dilution of 1:100. Image from verified ...read more
Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody [NBP1-77397] - HSP60 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescence analysis of HeLa cells using Hsp60 antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).
Simple Western: HSP60 Antibody [NBP1-77397] - Simple Western lane view shows a specific band for Hsp60 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, Mu, Rt, Ha, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
1.0 mg/ml

HSP60 Antibody Summary

A synthetic peptide made to an internal region of the human Hsp60 protein (within residues 70-150). [Swiss-Prot P10809]
Mitochondrion matrix
Mitochondria Marker
Predicted Species
Bovine (100%), Chicken (100%)
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol.
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
This Hsp60 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~61 kDa representing Hsp60. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 4 Reviews rated 5
NBP1-77397 in the following application:

Reactivity Notes

Human, mouse, rat, hamster and primate. Immunogen has 100% identity to bovine and chicken.

Alternate Names for HSP60 Antibody

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • Hsp60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13


Heat shock protein 60 activates T cells and ultimately autoimmune actions and diseases. In gastric cancer, HSP60 is upregulated in malignant ascites that have been treated, and induces a tumor-specific cytotoxic T lymphocyte response. Enhancing Hsp60 expression is beneficial for protecting bone tissue against osteoblast apoptosis. Hsp60 is highly influenced by dietary intake of antioxidants, and is a prime target for many natural therapies against gut inflammation, preeclampsia, and some low level bacterial infections. Ischemia and reperfusion, as well as hypoxic environments can cause temporary elevations in Hsp60 expression levels.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Bv, Ca, GP, Rb, Sh, Xp
Applications: WB, ELISA, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ch, Dr, Pm
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC
Species: Mu
Applications: Flow, ICC
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, Sh
Applications: WB, EM, EIA, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ELISA(Sta)
Species: Mu
Applications: WB, IHC, ICC

Publications for HSP60 Antibody (NBP1-77397) (0)

There are no publications for HSP60 Antibody (NBP1-77397).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSP60 Antibody (NBP1-77397) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 3 application: Flow, ICC, WB.

Reviews using NBP1-77397:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Flow Cytometry HSP60 NBP1-77397
reviewed by:
Flow Mouse 12/30/2014


ApplicationFlow Cytometry
Sample TestedMurine prostate cancer cell line RM1


Blocking DetailsFc blocking antibody for 10' at 4C

Primary Anitbody

Dilution Ratio1:100 in facs buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG-PE
Secondary Concentration1:400


Detection NotesFACS Diva acquisition with a LSRII machine and FlowJo software for analysis
Permeabilization DetailsD Cytofix/Cytoperm™ Kit (Cat. No. 554714) 20' at 4C
Immunocytochemistry HSP60 NBP1-77397
reviewed by:
ICC Human 06/05/2014


Special ApplicationsConfocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C). This Hsp60 antibody detected Hsp60 at cytoplasm of HeLa cells in confocal immunofluorescent analysis at dilution of 1:100.
FileView PDF
Western Blot HSP60 NBP1-77397
reviewed by:
WB Human 06/05/2014


ApplicationWestern Blot
Special ApplicationsWestern blot analysis of extracts from HeLa and COS cells using Hsp60 antibody (NBP1-77397, 1:2000). This antibody detected endogenous levels of Hsp60 in HeLa and COS cell lysates in WB at dilution of 1:2000 (0.5 ug/ml).
Immunocytochemistry HSP60 NBP1-77397
reviewed by:
ICC Human 05/21/2014


FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP60 Antibody (NBP1-77397) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-77397AF405
Alexa Fluor (R) 488 Labeled NBP1-77397AF488
Alexa Fluor (R) 647 Labeled NBP1-77397AF647
Alexa Fluor (R) 700 Labeled NBP1-77397AF700
Biotin Labeled NBP1-77397B
DyLight 405 Labeled NBP1-77397V
DyLight 488 Labeled NBP1-77397G
DyLight 550 Labeled NBP1-77397R
DyLight 650 Labeled NBP1-77397C
FITC Labeled NBP1-77397F
HRP Labeled NBP1-77397H

Additional HSP60 Antibody Products

Related Products by Gene

Bioinformatics Tool for HSP60 Antibody (NBP1-77397)

Discover related pathways, diseases and genes to HSP60 Antibody (NBP1-77397). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HSP60 Antibody (NBP1-77397)

Discover more about diseases related to HSP60 Antibody (NBP1-77397).

Pathways for HSP60 Antibody (NBP1-77397)

View related products by pathway.

PTMs for HSP60 Antibody (NBP1-77397)

Learn more about PTMs related to HSP60 Antibody (NBP1-77397).

Research Areas for HSP60 Antibody (NBP1-77397)

Find related products by research area.

Blogs on HSP60.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol HSPD1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-77397 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought