Human Heart Whole Tissue Lysate (Adult Whole Normal) Summary
Total Protein - Human Adult Normal Tissue: Heart
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Publications using NB820-59217 in the following applications:
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Lysate Details for Heart
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
for 6 months from date of receipt.
Publications for Heart Lysate (NB820-59217)(4)
We have publications tested in 1 confirmed species: Monkey.
We have publications tested in 1 application: WB.
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Heart Lysate (NB820-59217) (0)
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Product General Protocols
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FAQs for Heart Lysate (NB820-59217). (Showing 1 -
3 of 3 FAQs).
Is this lysate a whole heart tissue or a particular heart myocard area - ventricle / atrium?
NB820-59217 is a lysate from the whole heart.
If I want to dilute the 20ug so it will give a volume larger than 4ul as supplied, what buffer do you recommend to use for that?
We recommend diluting in the buffer it comes supplied in, HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
How much protein do you recommend to use for each WB well for best results with a standard gel of 12 wells?
We recommend loading 10 to 20 ug per well.
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