Human Heart Whole Tissue Lysate (Adult Whole Normal)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Heart Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Heart

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Publications
Read Publications using
NB820-59217 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration
5.0 mg/ml

Lysate Details for Heart

Type
Tissue
Life Stage
Adult
Tissue
Heart
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Heart Lysate (NB820-59217)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Heart Lysate (NB820-59217). (Showing 1 - 3 of 3 FAQs).

  1. Is this lysate a whole heart tissue or a particular heart myocard area - ventricle / atrium?
    • NB820-59217 is a lysate from the whole heart.
  2. If I want to dilute the 20ug so it will give a volume larger than 4ul as supplied, what buffer do you recommend to use for that?
    • We recommend diluting in the buffer it comes supplied in, HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
  3. How much protein do you recommend to use for each WB well for best results with a standard gel of 12 wells?
    • We recommend loading 10 to 20 ug per well.

Additional Heart Products

Heart NB820-59217

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