Human Heart Whole Tissue Lysate (Adult Whole Normal) Summary
Total Protein - Human Adult Normal Tissue: Heart
Immunoprecipitation Western Blot
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Publications using NB820-59217 in the following applications:
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Lysate Details for Array
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
for 6 months from date of receipt.
Publications for Heart Lysate (NB820-59217)(6)
We have publications tested in 2 confirmed species: Human, Monkey.
We have publications tested in 2 applications: MS, WB.
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Showing Publications 1 -
Publications using NB820-59217
Dube Dk, Dube S, Abbott L Et Al. Sarcomeric TPM3 expression in human heart and skeletal muscle Cytoskeleton (Hoboken) Aug 5 2020 [PMID: 32761805]
Lau E, Han Y, Williams DR, et al. Splice-Junction-Based Mapping of Alternative Isoforms in the Human Proteome Cell Rep Dec 10 2019 [PMID: 31825849] (MS, Human) MS
Duka T, Collins Z, Anderson SM et al. Divergent lactate dehydrogenase isoenzyme profile in cellular compartments of primate forebrain structures. Mol. Cell. Neurosci. Apr 29 2017 [PMID: 28461219] (Monkey)
Dube S, Thomas A, Abbott L et al. Expression of tropomyosin 2 gene isoforms in human breast cancer cell lines. Oncol. Rep. Jun 1 2016 [PMID: 27108600]
Duka T, Anderson SM, Collins Z et al. Synaptosomal Lactate Dehydrogenase Isoenzyme Composition Is Shifted toward Aerobic Forms in Primate Brain Evolution. Brain Behav. Evol. 2014 Mar 28 [PMID: 24686273]
Peterfi Z, Toth ZE, Kovacs HA et al. Peroxidasin-like protein: a novel peroxidase homologue in the human heart. Cardiovasc Res 2013 Dec 24 [PMID: 24253521] (WB) WB
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Heart Lysate (NB820-59217). (Showing 1 -
4 of 4 FAQs).
Is this lysate a whole heart tissue or a particular heart myocard area - ventricle / atrium?
NB820-59217 is a lysate from the whole heart.
If I want to dilute the 20ug so it will give a volume larger than 4ul as supplied, what buffer do you recommend to use for that?
We recommend diluting in the buffer it comes supplied in, HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
How much protein do you recommend to use for each WB well for best results with a standard gel of 12 wells?
We recommend loading 10 to 20 ug per well.
I was looking at NB820-59217 and I would like to know if it is denatured at all?
They will not be native since they were lysed in this buffer:HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitorswith detergents
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