HSP90 Antibody (AC88)

Western Blot: HSP90 Antibody (AC88) [NB100-1972] - Analysis of HSP90, mAb (AC88): Lane 1: MW Marker; Lane 2: HSP90 (human), (native) ; Lane 3: HSP90 beta, (human) (recombinant); Lane 4: HSP90a (human), (recombinant); Lane 5: HeLa (heat shocked); Lane 6: 3T3 (heat shocked); Lane 7: PC-12 (heat shocked) ; Lane 8: CHO-K1 (heat shocked).

Immunohistochemistry: HSP90 Antibody (AC88) [NB100-1972] - Analysis of human spleen tissue stained with HSP90, mAb (AC88) at 10 ug/ml.

Flow (Intracellular): HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE format of this antibody.

Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the DyLight 488 conjugate of NB100-1972. Staining of 106 Jurkat cells using HSP90 mAb (AC88), DyLight 488 Conjugate at a concentration of 50ug/ml.

Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the Alexa Fluor 405 conjugated HSP90 antibody, NB100-1972AF405 (purple) and fluorescence minus one control (orange). Staining of HSP90 in Primary CD4+ T-cells derived from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.

Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the PE conjugate of NB100-1972. Staining of 10^6 Jurkat cells using HSP90 mAb (AC88), R-Phycoerythrin Conjugate at a concentration of 10ug/mL

Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on Jurkat cells with HSP90 Antibody (AC88) NB100-1972AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu, Mu, Rt, Po, Ba, Ca, Ch, Fi, Gp, Pm, Rb, Sh
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clone: AC88
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

HSP90 Antibody (AC88) Summary

• Immunogen: Achlya ambisexualis (water mold) Hsp90.
• Epitope: The epitope of this antibody has been mapped to amino acid residues 604-697 of the human Hsp90 sequence.
• Localization: Cytoplasmic
• Specificity: Recognizes both Hsp90 alpha and beta. It reacts with the rodent glucocorticoid receptor and with proliferation potential proteins (P2PS) with apparent molecular masses of 30-40 kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSP90AA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1 ug/ml
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 30373228).
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Recognizes Beluga, C. elegans, Mussel, Scallop and Water Mold HSP90. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HSP90 Antibody (AC88)

• FLJ31884
• Heat shock 86 kDa
• heat shock 90kD protein 1, alpha
• heat shock 90kD protein 1, alpha-like 4
• heat shock 90kD protein, alpha-like 4
• heat shock 90kDa protein 1, alpha
• heat shock protein 90kDa alpha (cytosolic), class A member 1
• heat shock protein HSP 90-alpha
• Hsp89
• HSP90
• HSP90AHSP86
• HSP90N
• HSPC1HSP 86
• HSPCAHSP89A
• HSPCAL1
• HSPCAL4
• HSPN
• LAP2
• Renal carcinoma antigen NY-REN-38

Background

In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP90 Antibody (NB100-1972)(6)

Publications using NB100-1972

• Panagioti E, Kurokawa C, Viker K Et Al. Immunostimulatory bacterial antigen-armed oncolytic measles virotherapy significantly increases the potency of anti-PD1 checkpoint therapy The Journal of clinical investigation 2021-07-01 [PMID: 34196308] (FLOW)
• Freund E, Miebach L, Stope MB, Bekeschus S Hypochlorous acid selectively promotes toxicity and the expression of danger signals in human abdominal cancer cells Oncology reports 2021-05-01 [PMID: 33760187] (FLOW, Human)
• Mohamed H, Clemen R, Freund E et al. Non-thermal plasma modulates cellular markers associated with immunogenicity in a model of latent HIV-1 infection PloS one 2021-03-01 [PMID: 33647028]
• Bekeschus S, Clemen R, Niebner F et al. Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion Adv. Sci. 2020-03-30 [PMID: 32440479]
• Bekeschus S, Freund E, Wende k et al. Hmox1 Upregulation Is a Mutual Marker in Human Tumor Cells Exposed to Physical Plasma-Derived Oxidants. Antioxidants (Basel). 2018-10-27 [PMID: 30373228] (ICC/IF, Human)
• Kelly RL, Geoghegan JC, Feldman J et al. Chaperone proteins as single component reagents to assess antibody nonspecificity MAbs 2017-07-26 [PMID: 28745541] (Mouse)

Bioinformatics

• Gene Symbol: HSP90AA1
• Entrez:
  • 3320(Human)
  • 15519(Mouse)
  • 299331(Rat)