HSPA8/HSC71/Hsc70 Antibody (13D3) Summary
Immunogen |
Mouse spermatogenic cell protein. |
Specificity |
This displays slight cross-reactivity to HSP 70. |
Isotype |
IgM |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
HSPA8 |
Purity |
Unpurified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Block/Neutralize
- Flow Cytometry 1:10 - 1:1000
- Immunoblotting
- Immunocytochemistry/Immunofluorescence 1:20 - 1:200
- Immunohistochemistry 1:20 - 1:200
- Immunohistochemistry-Frozen 1:20 - 1:200
- Immunohistochemistry-Paraffin 1:20 - 1:200
- Immunoprecipitation 2 ul
- Western Blot 1:1000 - 1:5000
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Application Notes |
WB: Detects an approx. 70 kDa protein representing Hsc 70. By 2D gel electrophoresis, this antibody binds strongly to Hsc 70 and faintly to HSP 70 both before and after heat shock. IHC: Staining of Hsc 70 in mouse spermatids/spermatozoa results in staining restricted to the post acrosomal region in condensing spermatids and to the midpiece in spermatozoa. May be useful in IHC-Fr. Use in Immunoblotting reported in scientific literature (PMID 28095639). |
Theoretical MW |
70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Publications |
Read Publications using NB120-2788 in the following applications:
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Reactivity Notes
Reported cross-reactivity with Bovine in literature (Terlecky et al.). Hamster reactivity reported in scientific literature (PMID: 16737757). Fish reactivity reported in scientific literature (PMID: 9006893). Rat reactivity reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
Ascites diluted with PBS |
Preservative |
0.05% Sodium Azide |
Purity |
Unpurified |
Alternate Names for HSPA8/HSC71/Hsc70 Antibody (13D3)
Background
Heat shock proteins (HSP's) or stress response proteins (SRP's) are synthesized in variety of environmental and pathophysiological stressful conditions. Many HSP's are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors,actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for HSPA8/HSC71/Hsc70 Antibody (NB120-2788). (Showing 1 - 2 of 2 FAQs).
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What is the concentration of this product?
- As this product is sold as unpurified ascites, the concentration of the antibody has not been determined.
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Would any endotoxin and azide exist in buffer?
- The antibody solution contains 0.05% sodium azide. We have not tested this product for endotoxin levels.
Secondary Antibodies
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Isotype Controls
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