HSPA8/HSC71/Hsc70 Antibody (13D3)

Images

 
Western Blot: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody at a ...read more
Immunocytochemistry/ Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using Monoclonal antibody (13D3) shows staining in NIH-3T3 cells. HSC70 staining (green), F-Actin staining with ...read more
Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Prostate carcinoma tissue.
Immunocytochemistry/ Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker ...read more
Immunocytochemistry/ Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in HeLa cells. HSC70 staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in MCF-7 cells. HSC70 staining (green), F-Actin staining with ...read more
Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.
Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Testis tissue.
Immunoprecipitation: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug whole cell lysate with 2ul of HSC70 monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, Fi, Ha, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, WB, B/N
Clone
13D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HSPA8/HSC71/Hsc70 Antibody (13D3) Summary

Immunogen
Mouse spermatogenic cell protein.
Specificity
This displays slight cross-reactivity to HSP 70.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
HSPA8
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry 1:10 - 1:1000
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:20 - 1:200
  • Immunohistochemistry-Frozen 1:20 - 1:200
  • Immunohistochemistry-Paraffin 1:20 - 1:200
  • Immunoprecipitation 2 ul
  • Simple Western reported by internal validation
  • Western Blot 1:1000 - 1:5000
Application Notes
WB: Detects an approx. 70 kDa protein representing Hsc 70. By 2D gel electrophoresis, this antibody binds strongly to Hsc 70 and faintly to HSP 70 both before and after heat shock. IHC: Staining of Hsc 70 in mouse spermatids/spermatozoa results in staining restricted to the post acrosomal region in condensing spermatids and to the midpiece in spermatozoa. May be useful in IHC-Fr. Use in Immunoblotting reported in scientific literature (PMID 28095639). In Simple Western internal validation: Mouse Brain lysate as sample; separated by size; antibody dilution of 1:2000; matrix was 12-230 kDa; detected by Chemiluminescence.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB120-2788 in the following applications:

Publications
Read Publications using
NB120-2788 in the following applications:

  • 1 publication
  • IB
    1 publication
  • 3 publications
  • 1 publication
  • 1 publication
  • IP
    3 publications
  • WB
    11 publications

Reactivity Notes

Reported cross-reactivity with Bovine in literature (Terlecky et al.). Hamster reactivity reported in scientific literature (PMID: 16737757). Fish reactivity reported in scientific literature (PMID: 9006893). Rat reactivity reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for HSPA8/HSC71/Hsc70 Antibody (13D3)

  • Heat shock 70 kDa protein 8
  • heat shock 70kD protein 8
  • heat shock 70kDa protein 8
  • heat shock cognate 71 kDa protein
  • heat shock cognate protein 54
  • heat shock cognate protein, 71-kDa
  • HSC54
  • HSC70
  • HSC70heat shock 70kd protein 10
  • HSC71
  • HSP71
  • HSP73
  • HSP73constitutive heat shock protein 70
  • HSPA10
  • HSPA8
  • LAP1
  • lipopolysaccharide-associated protein 1
  • LPS-associated protein 1
  • MGC131511
  • MGC29929
  • NIP71
  • N-myristoyltransferase inhibitor protein 71

Background

Heat shock proteins (HSP's) or stress response proteins (SRP's) are synthesized in variety of environmental and pathophysiological stressful conditions. Many HSP's are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors,actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSPA8/HSC71/Hsc70 Antibody (NB120-2788)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: FLOW, IB, ICC/IF, IF/IHC, IHC-P, IP, WB.


Filter By Application
FLOW
(1)
IB
(1)
ICC/IF
(3)
IF/IHC
(1)
IHC-P
(1)
IP
(3)
WB
(11)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
Rat
(4)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB120-2788 Applications Species
Li J, Yao H, Zhao F et al. Pycard deficiency inhibits microRNA maturation and prevents neointima formation by promoting chaperone-mediated autophagic degradation of AGO2/argonaute 2 in adipose tissue Autophagy 2023-11-14 [PMID: 37963060] (IHC-P, IP, Mouse)

Details:
IHC-P Dilution 1:300; IP Dilution: 1?µg of antibody was added to 500?µg of cell lysate and incubated overnight at 4°C, and then incubated with protein A agarose beads for 4?h
IHC-P, IP Mouse
Choi YJ, Yun SH, Yu J et al. Chaperone-mediated autophagy dysregulation during aging impairs hepatic fatty acid oxidation via accumulation of NCoR1 Molecular metabolism 2023-07-29 [PMID: 37524243] (WB) WB
Idera, A;Sharkey, LM;Kurauchi, Y;Kadoyama, K;Paulson, HL;Katsuki, H;Seki, T; Wild-type and pathogenic forms of ubiquilin 2 differentially modulate components of the autophagy-lysosome pathways Journal of pharmacological sciences 2023-07-01 [PMID: 37257946] (WB, Human) WB Human
Ueda E, Ohta T, Konno A et al. D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation Cells 2022-04-05 [PMID: 35406792] (WB) WB
Wang D, Tan KS, Zeng W et al. Hepatocellular BChE as a therapeutic target to ameliorate hypercholesterolemia through PRMT5 selective degradation to restore LDL receptor transcription Life sciences 2022-01-19 [PMID: 35065166]
Bourdenx M, Mart�n-Segura A, Scrivo A et al. Chaperone-mediated autophagy prevents collapse of the neuronal metastable proteome Cell 2021-05-01 [PMID: 33891876]
Caballero B, Bourdenx M, Luengo E et al. Acetylated tau inhibits chaperone-mediated autophagy and promotes tau pathology propagation in mice Nature communications 2021-04-14 [PMID: 33854069]
Juste YR, Cuervo AM. Analysis of Chaperone-Mediated Autophagy. Methods Mol. Biol. 2019-01-06 [PMID: 30610733] (ICC/IF, Rat) ICC/IF Rat
Seki T, Sato M, Kibe Y et al. Lysosomal dysfunction and early glial activation are involved in the pathogenesis of spinocerebellar ataxia type 21 caused by mutant transmembrane protein 240. Neurobiol. Dis. 2018-09-02 [PMID: 30184469] (WB, Human, Rat, Mouse) WB Human, Rat, Mouse
Pajares M, Rojo AI, Arias E et al. Transcription factor NFE2L2/NRF2 modulates chaperone-mediated autophagy through the regulation of LAMP2A Autophagy 2018-07-26 [PMID: 29950142] (WB, Human) WB Human
Show All 26 Publications.

Reviews for HSPA8/HSC71/Hsc70 Antibody (NB120-2788) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB120-2788:
Filter by Applications
Simple Western
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western HSPA8/HSC71/Hsc70 NB120-2788
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3
reviewed by:
Verified Customer
Simple Western Mouse 01/21/2016
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Summary

ApplicationSimple Western
Sample Testedwhole brain homogenates from mice
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Human 02/04/2009
View

Summary

ApplicationWestern Blot
Sample TestedHEK293 cell lysate, Sample Amount: 20 ug
SpeciesHuman
Lot056-114

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSPA8/HSC71/Hsc70 Antibody (NB120-2788). (Showing 1 - 2 of 2 FAQs).

  1. What is the concentration of this product?
    • As this product is sold as unpurified ascites, the concentration of the antibody has not been determined.
  2. Would any endotoxin and azide exist in buffer?
    • The antibody solution contains 0.05% sodium azide. We have not tested this product for endotoxin levels.

Secondary Antibodies

 

Isotype Controls

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Blogs on HSPA8/HSC71/Hsc70.

Chaperone Mediated Autophagy (CMA) does it all!
By Christina Towers, PhD. The degradation of cellular proteins is a critical step of both regulation and quality control and results in the turn over and recycling of critical amino acids. The two main mechanisms o...  Read full blog post.

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Recent Reviews

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Verified Customer
01/21/2016
Application: Simple Western
Species: Mouse

 
Verified Customer
02/04/2009
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSPA8
Uniprot