HspA1B Antibody (3B7)

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Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7. Analysis of HSPA1B expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: HspA1B Antibody (3B7) [H00003304-M02] - Analysis of monoclonal antibody to HSPA1B on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: HspA1B Antibody (3B7) [H00003304-M02] - Analysis of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. Antibody concentration 6 ug/ml.
Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7. Analysis of HSPA1B expression in PC-12.
Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7 Analysis of HSPA1B expression in IMR-32.
Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7. Analysis of HSPA1B expression in Raw 264.7.
Proximity Ligation Assay: HspA1B Antibody (3B7) [H00003304-M02] - Analysis of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and ...read more
ELISA: HspA1B Antibody (3B7) [H00003304-M02] - Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA
Clone
3B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HspA1B Antibody (3B7) Summary

Immunogen
HSPA1B (NP_005336, 531 a.a. ~ 611 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLY
Specificity
HSPA1B (3B7)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1B
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Dot Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Sandwich ELISA
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. Use in Dot blot reported in scientific literature (PMID: 26344128).
Publications
Read Publications using
H00003304-M02 in the following applications:

  • DB
    1 publication
  • 1 publication
  • WB
    2 publications

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26344128). Porcine reactivity reported in scientific literature (PMID: 24862986). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HspA1B Antibody (3B7)

  • FLJ54328
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1B
  • heat shock 70kDa protein 1B
  • HSP70.1/HSP70.2
  • HSP70-1/HSP70-2
  • HSP70-1B
  • HSP70-2
  • HSPA1
  • HSPA1A

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HspA1B Antibody (H00003304-M02)(6)

We have publications tested in 3 confirmed species: Mouse, Bovine, Porcine.

We have publications tested in 3 applications: DB, ICC/IF, WB.


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DB
(1)
ICC/IF
(1)
WB
(2)
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Mouse
(1)
Bovine
(1)
Porcine
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HSPA1B
Entrez
OMIM
Uniprot