HDAC11 Antibody - BSA Free

Western Blot: HDAC11 Antibody [NB100-56382] - Analysis of HDAC11 using this antibody. Brain lysate from A) human, B) mouse and C) rat brain probed with this antibody at 2 ug/ml.

Simple Western: HDAC11 Antibody [NB100-56382] - 1:25 dilution against the indicated concentration of protein lysate from human SVG-A cells. Image from verified customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

HDAC11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HDAC11 Antibody - BSA Free (NB100-56382) is a polyclonal antibody validated for use in WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed by immunizing mice with a synthetic peptide (AHQGNGHERDFMDDKRVYI) of human HDAC11.
• Specificity: This peptide sequence is 100% homologous in multiple HDAC11 isoforms corresponding to amino acids 60-78. The observed molecular weight in western blots can vary depending on the isoform(s) detected. Users are encouraged to refer to NBCI data bases for more information about HDAC-11 isoforms and their amino acid size.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC11
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:25
  • Western Blot 1-3 ug/ml
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Human SVG-A cell protein lysate, separated by Size, antibody dilution of 1:25
• Control:

  HDAC11 Overexpression Lysate
  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for HDAC11 Antibody - BSA Free

• EC 3.5.1.98
• FLJ22237
• HD11
• HDAC11
• histone deacetylase 11

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns. Recently, a new member of this family, HDAC11, has been cloned by Gao et al. HDAC11 transcripts were limited to kidney, heart, brain, skeletal muscle, and testis. These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for HDAC11 Antibody (NB100-56382) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 03/08/2019

Summary

• Application: Simple Western
• Sample Tested: SVG-A whole cell lysate
• Species: Human
• Lot: IN072804D-03

Bioinformatics

• Gene Symbol: HDAC11
• Entrez:
  • 79885(Human)
  • 232232(Mouse)
  • 297453(Rat)
• Uniprot:
  • Q9UKV0(Human)