HDAC5 Antibody

Images

 
Biological Strategies: Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.
Western Blot: HDAC5 Antibody [NBP2-22152] - WB analysis of HDAC5 in 1. HeLa, 2. Ntera 2, 3. HepG2 and 4. MCF7 cell lysates.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

HDAC5 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human HDAC5 protein (between residues 1-100) [UniProt Q9UQL6]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 - 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50 - 1:100
Application Notes
This HDAC5 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 130 kDa. In ICC/IF nuclear staining was observed in A431 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MCF-7 Whole Cell Lysate
HeLa Whole Cell Lysate
HDAC5 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22152 in the following applications:

Publications
Read Publications using
NBP2-22152 in the following applications:

Reactivity Notes

Human, mouse and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC5 Antibody

  • Antigen NY-CO-9
  • EC 3.5.1.98
  • FLJ90614
  • HD5
  • histone deacetylase 5
  • KIAA0600
  • NY-CO-9

Background

HDAC5, or histone deacetylase mHDA1, is responsible for the deacetylation of lysine residues on the N-terminal end of the core histones H2A, H2B, H3 and H4. Identifying histone acetyltransferases reveals much needed insight on how these enzymes regulate transcription, and further elucidates their pivotal role in cell cycle progression and other developmental events. HDAC5 shuttles between the cytoplasm and nucleus, depending on its nuclear export sequence, and shares interactions with BAHD1, KDM5B and MEF family proteins. While HDAC5 has many biological implications, recent studies have proposed its key involvement in synaptic plasticity and memory formation (PMID: 22914591), as well as its connection to various cancers (PMID: 22301920, PMID: 22944197).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-22152
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P

Publications for HDAC5 Antibody (NBP2-22152)(2)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ChIP, IHC-P.


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Showing Publications 1 - 2 of 2.
Publications using NBP2-22152 Applications Species
Baek MH, Park JY, Rhim CC et al. Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma. Anticancer Res. May 1 2016 [PMID: 27127168] (IHC-P, Human) IHC-P Human
Kim H, Lee J, Lee H et al. Meteorin-like (Metrnl) adipomyokine improves glucose tolerance in type 2 diabetes via AMPK pathway. bioRxiv Sep 19 2018 (ChIP, Mouse, Rat) ChIP Mouse, Rat

Review for HDAC5 Antibody (NBP2-22152) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-22152:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot HDAC5 NBP2-22152
Enlarge
reviewed by:
Liyong Zhang
WB Human 08/15/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HDAC5 Antibody (NBP2-22152). (Showing 1 - 1 of 1 FAQs).

  1. Do the following antibodies  cross-react with any other the HDAC's? 1) NBP2-22152 and 2) NBP2-16795
    • We have not actually tested these for cross reactivity.   I have looked at homology between he HDAC5/HDAC7 proteins and they are generally only about 60% homologous. As polyclonal antibodies they may cross react slightly.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22152AF350
Alexa Fluor 405 NBP2-22152AF405
Alexa Fluor 488 NBP2-22152AF488
Alexa Fluor 532 NBP2-22152AF532
Alexa Fluor 594 NBP2-22152AF594
Alexa Fluor 647 NBP2-22152AF647
Alexa Fluor 700 NBP2-22152AF700
Alexa Fluor 750 NBP2-22152AF750
Biotin NBP2-22152B
DyLight 350 NBP2-22152UV
DyLight 405 NBP2-22152V
DyLight 488 NBP2-22152G
DyLight 550 NBP2-22152R
DyLight 594 NBP2-22152DL594
DyLight 650 NBP2-22152C
DyLight 680 NBP2-22152FR
DyLight 755 NBP2-22152IR
FITC NBP2-22152F
HRP NBP2-22152H
Janelia Fluor 549 NBP2-22152JF549
Janelia Fluor 646 NBP2-22152JF646

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Recent Reviews

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Liyong Zhang
08/15/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HDAC5
Entrez
Uniprot