HDAC6 Antibody

Images

 
Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.
Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated ...read more
Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

HDAC6 Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of human HDAC6 (between amino acids 1-50) [UniProt Q9UBN7]
Specificity
In NIH 3T3, a 134 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 5 ug/ml
  • Simple Western 1:20
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml
  • Immunoprecipitation assay dependent
Application Notes
Chromatin Immunoprecipitation: Refer to Imbriano (2005) for details.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
NIH 3T3 Whole Cell Lysate
HDAC6 Overexpression Lysate
HDAC6 Knockout HeLa Cell Lysate
Publications
Read Publication using NB100-56343.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC6 Antibody

  • EC 3.5.1.98
  • HD6FLJ16239
  • histone deacetylase 6
  • JM21
  • KIAA0901

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC6 Antibody (NB100-56343)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HDAC6 Antibody (NB100-56343) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-56343AF405
Alexa Fluor 488 NB100-56343AF488
Alexa Fluor 532 NB100-56343AF532
Alexa Fluor 647 NB100-56343AF647
Alexa Fluor 700 NB100-56343AF700
Biotin NB100-56343B
DyLight 350 NB100-56343UV
DyLight 405 NB100-56343V
DyLight 488 NB100-56343G
DyLight 550 NB100-56343R
DyLight 594 NB100-56343DL594
DyLight 650 NB100-56343C
DyLight 680 NB100-56343FR
DyLight 755 NB100-56343IR
HRP NB100-56343H

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Bioinformatics

Gene Symbol HDAC6
Entrez