HDAC6 Antibody

Images

 
Western Blot: HDAC6 Antibody [NB100-56343] - analysis of HDAC-6 in NIH 3T3 cell lysate with this antibody.
Simple Western: HDAC6 Antibody [NB100-56343] - Simple Western lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HDAC6 Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 1-16 of human HDAC6.
Specificity
In NIH 3T3, a 134 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC6
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 5 ug/ml
  • Simple Western 1:20
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunoprecipitation assay dependent
Application Notes
Chromatin Immunoprecipitation: Refer to Imbriano (2005) for details.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
HDAC6 Lysate (NBL1-11484)
Publications
Read Publication using NB100-56343.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HDAC6 Antibody

  • EC 3.5.1.98
  • HD6FLJ16239
  • histone deacetylase 6
  • JM21
  • KIAA0901

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC6 Antibody (NB100-56343)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HDAC6
Entrez
Uniprot