MBP Antibody (12)

Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.

Immunocytochemistry/Immunofluorescence: MBP Antibody (12) [NB600-717] - Histological and mRNA analysis of the inflammatory cytokines in the vehicle- or etifoxine-treated mice at onset of clinical symptoms. At day 10 p.i., drug treated animals showed significant differences in MBP staining. Animals treated with etifoxine showed an increase in retention of percentage of MBP coverage (*p = 0.001). Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201202124), licensed under a CC-BY license.

Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.

Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Gp, Ma, Rb, Sh
• Applications: WB, ELISA, Func, ICC/IF, IHC
• Clone: 12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

MBP Antibody (12) Summary

• Immunogen: Bovine MBP
• Epitope: Amino acids 82-87.
• Localization: Cytoplasmic side of myelin
• Marker: Oligodendrocyte Marker, Myelin Marker
• Specificity: NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: MBP
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Radioimmunodiffusion
  • Western Blot 1:100-1:2000

Reactivity Notes

Canine reactivity reported in scientific literature (PMID:32773406). Feline reactivity reported in scientific literature (PMID:33091431).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 M Tris
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for MBP Antibody (12)

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBP Antibody (NB600-717)(34)

Publications using NB600-717

• Lin Q, Hu DW, Hao XH et al. Effect of Hypoxia-Ischemia on the Expression of Iron-Related Proteins in Neonatal Rat Brains Neural plasticity 2023-04-20 [PMID: 37124874] (WB, Rat)
• Macintosh J, Michell-Robinson M, Chen X et al. An optimized and validated protocol for the purification of PDGFR?+ oligodendrocyte precursor cells from mouse brain tissue via immunopanning MethodsX 2023-02-01 [PMID: 36814689]
• Sohel MSH, Homma T, Onouchi S, Saito S Expression patterns of prosaposin and its receptors, G protein-coupled receptor (GPR) 37 and GPR37L1 mRNAs, in the chick inner ear Cell and tissue research 2023-02-08 [PMID: 36750499]
• Mohammad Rk, Sheikh FA, Ahmed N et al Cathepsin-B inhibitor CA-074 attenuates retinopathy and optic neuritis in experimental autoimmune encephalomyelitis induced in SJL/J mice Saudi Pharmaceutical Journal 2022-11-01 [PMID: 36685301] (IHC-Fr, WB, Mouse)
  
  Details:
  WB Dilutions: 1:500; IHC-Fr Dilutions: 1:300
• Li J, Liang J, Zeng M et al. Oxymatrine ameliorates white matter injury by modulating gut microbiota after intracerebral hemorrhage in mice Research Square 2022-10-10 [PMID: 36550632] (ICC/IF, Mouse)
• Radojevic D, Bekic M, Gruden-Movsesijan A et al. Myeloid-derived suppressor cells prevent disruption of the gut barrier, preserve microbiota composition, and potentiate immunoregulatory pathways in a rat model of experimental autoimmune encephalomyelitis Gut microbes 2022-10-02 [PMID: 36184742] (IHC-P, Rat)
• Cheng Y, Shi L, Mao H et al. The Effect of Sevoflurane Anesthesia on the Biomarkers of Neural Injury in the Prefrontal Cortex of Aged Marmosets Frontiers in aging neuroscience 2022-06-30 [PMID: 35847680] (IHC, Primate)
• Hou Y, Luo D, Hou Y et al. Bu Shen Huo Xue decoction promotes functional recovery in spinal cord injury mice by improving the microenvironment to promote axonal regeneration Chinese medicine 2022-07-12 [PMID: 35820953]
• Xiao L, Zheng H, Li J et al. Targeting NLRP3 inflammasome modulates gut microbiota, attenuates corticospinal tract injury and ameliorates neurobehavioral deficits after intracerebral hemorrhage in mice Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-03-10 [PMID: 35279596] (IF/IHC, Mouse)
• Helbing DL, Bohm L, Oraha N et al. A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples Gels (Basel, Switzerland) 2022-01-07 [PMID: 35049578] (WB, Mouse)
• Kim Y, Lee J, Hwang J, Suh H A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model J Vet Res 2021-12-17 [PMID: 34917843]
• Wang Y, Zhang Y, Zhang S Et al. PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination Cell reports 2021-10-05 [PMID: 34610310] (WB, IF/IHC, Mouse)
• Hosoya M, Fujioka M, Murayama AY Et al. Neuronal development in the cochlea of a nonhuman primate model, the common marmoset Developmental neurobiology 2021-09-21 [PMID: 34545999]
• Kerkenberg N, Wachsmuth L, Zhang M Et al. Brain microstructural changes in mice persist in adulthood and are modulated by the palmitoyl acyltransferase ZDHHC7 The European journal of neuroscience 2021-08-06 [PMID: 34355442] (IF/IHC, Mouse)
• Zhang, S, Wang, Y Et al. The Wnt Effector TCF7l2 Promotes Oligodendroglial Differentiation by Repressing Autocrine BMP4-Mediated Signaling. J Neurosci 2021-02-24 [PMID: 33452226] (WB, Human)
• Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline)
• Bradbury AM, Bagel JH, Nguyen D et al. Krabbe disease successfully treated via monotherapy of intrathecal gene therapy J. Clin. Invest. 2020-09-01 [PMID: 32773406] (IHC-P, Canine)
• Gorse kM, Lafrenaye AD. The Importance of Inter-Species Variation in Traumatic Brain Injury-Induced Alterations of Microglial-Axonal Interactions. Front Neurol. 2018-09-20 [PMID: 30294296] (IF/IHC, Rat)
• Zhang S, RaSai A, Wang Y et al. The Stem Cell Factor Sox2 Is a Positive Timer of Oligodendrocyte Development in the Postnatal Murine Spinal Cord. Mol. Neurobiol. 2018-04-05 [PMID: 29623612] (WB, Mouse)
• Zhang S, Zhu X, Gui X et al. Sox2 is essential for oligodendroglial proliferation and differentiation during postnatal brain myelination and CNS remyelination J. Neurosci. 2018-01-15 [PMID: 29335358] (Mouse)
• Lafrenaye AD, Todani M, Walker SA, Povlishock JT. Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pig. J Neuroinflammation 2015-01-01 [PMID: 26438203] (Bovine)
• Meade ML, Hoffmann A, Makley MK et al. Quantitative proteomic analysis of the brainstem following lethal sarin exposure Brain Res. 2015-04-01 [PMID: 25842371] (WB, Guinea Pig)
• Itoh T, Horiuchi M, Ikeda RH Jr et al. ZPK/DLK and MKK4 Form the Critical Gateway to Axotomy-Induced Motoneuron Death in Neonates. J Neurosci 2014-08-06 [PMID: 25100604] (Mouse)
• Pusic AD, Pusic KM, Clayton BL et al. IFNgamma-stimulated dendritic cell exosomes as a potential therapeutic for remyelination. J Neuroimmunol. 2013-11-09 [PMID: 24275061] (WB, Rat)
• Daugherty DJ, Selvaraj V, Chechneva OV et al. A TSPO ligand is protective in a Mouse model of multiple sclerosis. EMBO Mol Med 2013-06-01 [PMID: 23681668] (ICC/IF, IHC-Fr, Mouse)
• Kao Shyan-Yuan, Kempfle Judith S, Jensen Jane B et al. Loss of osteoprotegerin expression in the inner ear causes degeneRation of the cochlear nerve and sensorineural hearing loss. Neurobiology of Disease 2013-01-01 [PMID: 23607938] (IF/IHC, Mouse)
• Soundarapandian MM, Selvaraj V, Lo U-G et al. Zfp488 promotes oligodendrocyte differentiation of neural progenitor cells in adult mice after demyelination. [PMID: 22355521]
• Lee E, Chanamara S, Pleasure D, Soulika AM. IFN-gamma signaling in the central nervous system controls the course of experimental autoimmune encephalomyelitis independently of the localization and composition of inflammatory foci. Journal of Neuroi. [PMID: 22248039]
• Bertrand MJ, Lippens S, Staes A et al. cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4). PLoS One 2011-01-01 [PMID: 21931591] (IF/IHC, Human)
• Chechneva OV, Mayrhofer F, Daugherty DJ et al. Low dose dextromethorphan attenuates moderate experimental autoimmune encephalomyelitis by inhibiting NOX2 and reducing peripheral immune cells infiltration in the spinal cord. Neurobiol Dis;44(1):63-72. 2011-10-01 [PMID: 21704706]
• Itoh A, Horiuchi M, Wakayama K et al. ZPK/DLK, a mitogen-activated protein kinase kinase kinase, is a critical mediator of programmed cell death of motoneurons Comparative Study 2011-05-18 [PMID: 21593306] (IHC-Fr, Mouse)
• Horiuchi M, Maezawa I, Itoh A et al. Amyloid Beta 1-42 oligomer inhibits myelin sheet formation in vitro. Neurobiology of Aging 2012-03-12 [PMID: 20594620] (WB, ICC/IF, Rat)
• Kawai K, Itoh T, Itoh A et al. Maintenance of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK. Eur J Neurosci 2009-12-03 [PMID: 20128842] (IF/IHC, Mouse)

Reviews for MBP Antibody (NB600-717) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Mouse, Feline.

reviewed by: Nagendra Rai WB Mouse 04/13/2019

Summary

• Application: Western Blot
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: 180405

reviewed by: Nagendra Rai IHC-Fr Mouse 04/13/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: 180405

reviewed by: Kazuya Oikawa IHC-Fr Feline 12/08/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: brain and spinal cord
• Species: Feline
• Lot: 161031

Bioinformatics

• Gene Symbol: MBP
• Entrez:
  • 618684(Bovine)
  • 4155(Human)
  • 17196(Mouse)
  • 24547(Rat)
• Uniprot:
  • P02687(Bovine)