MBP Antibody (12)

Images

 
Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.
Immunocytochemistry/ Immunofluorescence: MBP Antibody (12) [NB600-717] - Histological and mRNA analysis of the inflammatory cytokines in the vehicle- or etifoxine-treated mice at onset of clinical symptoms. At day 10 ...read more
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Gp, Ma, Rb, ShSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
Clone
12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

MBP Antibody (12) Summary

Immunogen
Bovine MBP
Epitope
Amino acids 82-87.
Localization
Cytoplasmic side of myelin
Marker
Oligodendrocyte Marker, Myelin Marker
Specificity
NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MBP
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Radioimmunodiffusion
  • Western Blot 1:100-1:2000
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB600-717 in the following applications:

Publications
Read Publications using
NB600-717 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID:32773406). Feline reactivity reported in scientific literature (PMID:33091431).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 M Tris
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for MBP Antibody (12)

  • Hmbpr
  • MBP
  • MGC99675
  • mld
  • Myelin A1 protein
  • myelin basic protein
  • Myelin membrane encephalitogenic protein
  • shi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
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Publications for MBP Antibody (NB600-717)(25)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Canine, Feline, Guinea Pig, Porcine.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(7)
IHC-Fr
(2)
IHC-P
(1)
WB
(7)
All Applications
Filter By Species
Human
(2)
Mouse
(10)
Rat
(3)
Bovine
(1)
Canine
(1)
Feline
(1)
Guinea Pig
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB600-717 Applications Species
Helbing DL, Bohm L, Oraha N et al. A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples Gels (Basel, Switzerland) Jan 7 2022 [PMID: 35049578] (WB, Mouse) WB Mouse
Wang Y, Zhang Y, Zhang S Et al. PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination Cell reports Oct 5 2021 [PMID: 34610310] (WB, IHC, Mouse) WB, IHC Mouse
Hosoya M, Fujioka M, Murayama AY Et al. Neuronal development in the cochlea of a nonhuman primate model, the common marmoset Developmental neurobiology Sep 21 2021 [PMID: 34545999]
Kerkenberg N, Wachsmuth L, Zhang M Et al. Brain microstructural changes in mice persist in adulthood and are modulated by the palmitoyl acyltransferase ZDHHC7 The European journal of neuroscience Aug 6 2021 [PMID: 34355442] (IHC, Mouse) IHC Mouse
Zhang, S, Wang, Y Et al. The Wnt Effector TCF7l2 Promotes Oligodendroglial Differentiation by Repressing Autocrine BMP4-Mediated Signaling. J Neurosci Feb 24 2021 [PMID: 33452226] (WB, Human) WB Human
Bradbury AM, Bagel JH, Nguyen D et al. Krabbe disease successfully treated via monotherapy of intrathecal gene therapy J. Clin. Invest. Sep 1 2020 [PMID: 32773406] (IHC-P, Canine) IHC-P Canine
Kim Y, Lee J, Hwang J, Suh H A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model Journal of Veterinary Research Aug 19 2021 (IHC, Porcine) IHC Porcine
Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res Oct 19 2020 [PMID: 33091431] (ICC/IF, Feline) ICC/IF Feline
Gorse kM, Lafrenaye AD. The Importance of Inter-Species Variation in Traumatic Brain Injury-Induced Alterations of Microglial-Axonal Interactions. Front Neurol. Sep 20 2018 [PMID: 30294296] (IHC, Rat) IHC Rat
Show All 25 Publications.

Reviews for MBP Antibody (NB600-717) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Feline.

Reviews using NB600-717:
Filter by Applications
WB
(1)
IHC-Fr
(2)
All Applications
Filter by Species
Mouse
(2)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MBP NB600-717
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 04/13/2019
View

Summary

ApplicationWestern Blot
Sample Tested brain and spinal cord
SpeciesMouse
Lot180405
Immunohistochemistry-Frozen MBP NB600-717
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 04/13/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested brain and spinal cord
SpeciesMouse
Lot180405
Immunohistochemistry-Frozen MBP NB600-717
Enlarge
4
reviewed by:
Verified Customer
IHC-Fr Feline 12/08/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested brain and spinal cord
SpeciesFeline
Lot161031

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MBP Antibody (NB600-717) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MBP Antibody (NB600-717)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
04/13/2019
Application: WB
Species: Mouse

Verified Customer
04/13/2019
Application: IHC-Fr
Species: Mouse

Verified Customer
12/08/2017
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol MBP
Entrez
Uniprot