MBP Antibody (12)

Images

 
Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, GP, Ma, Rb, ShSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
Clone
12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

MBP Antibody (12) Summary

Immunogen
Bovine MBP
Epitope
Amino acids 82-87.
Localization
Cytoplasmic side of myelin
Marker
Oligodendrocyte Marker, Myelin Marker
Specificity
NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MBP
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Radioimmunodiffusion
Application Notes
Use in IHC-F was reported in a scientific publication (PMID: 23681668).
Reviewed Applications
Read 1 Review rated 4
using
NB600-717 in the following applications:

Publications
Read Publications using
NB600-717 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1M Tris
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for MBP Antibody (12)

  • Hmbpr
  • MBP
  • MGC99675
  • mld
  • Myelin A1 protein
  • myelin basic protein
  • Myelin membrane encephalitogenic protein
  • shi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MBP Antibody (NB600-717)(15)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Guinea Pig.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(2)
IHC
(3)
IHC-Fr
(1)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
Rat
(2)
Bovine
(1)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB600-717 Applications Species
Zhang S, RaSai A, Wang Y et al. The Stem Cell Factor Sox2 Is a Positive Timer of Oligodendrocyte Development in the Postnatal Murine Spinal Cord. Mol. Neurobiol. Apr 5 2018 [PMID: 29623612] (WB, Mouse) WB Mouse
Zhang S, Zhu X, Gui X et al. Sox2 is essential for oligodendroglial proliferation and differentiation during postnatal brain myelination and CNS remyelination J. Neurosci. 2018 Jan 15 [PMID: 29335358] (Mouse) Mouse
Lafrenaye AD, Todani M, Walker SA, Povlishock JT. Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pig. J Neuroinflammation 2015 [PMID: 26438203] (Bovine) Bovine
Meade ML, Hoffmann A, Makley MK et al. Quantitative proteomic analysis of the brainstem following lethal sarin exposure Brain Res. 2015 Apr 01 [PMID: 25842371] (WB, Guinea Pig) WB Guinea Pig
Itoh Takayuki, Horiuchi Makoto, Ikeda Raymond H Jr et al. ZPK/DLK and MKK4 form the critical gateway to axotomy-induced motoneuron death in neonates. J Neurosci. 2014 Aug 06 [PMID: 25100604] (Mouse) Mouse
Pusic AD, Pusic KM, Clayton BL et al. IFNgamma-stimulated dendritic cell exosomes as a potential therapeutic for remyelination. J Neuroimmunol. 2013 Nov 9 [PMID: 24275061] (WB, Rat) WB Rat
Daugherty DJ, Selvaraj V, Chechneva OV et al. A TSPO ligand is protective in a Mouse model of multiple sclerosis. EMBO Mol Med 2013 Jun [PMID: 23681668] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Kao Shyan-Yuan, Kempfle Judith S, Jensen Jane B et al. Loss of osteoprotegerin expression in the inner ear causes degeneRation of the cochlear nerve and sensorineural hearing loss. Neurobiology of Disease 2013 [PMID: 23607938] (IHC, Mouse) IHC Mouse
Soundarapandian MM, Selvaraj V, Lo U-G et al. Zfp488 promotes oligodendrocyte differentiation of neural progenitor cells in adult mice after demyelination. [PMID: 22355521]
Lee E, Chanamara S, Pleasure D, Soulika AM. IFN-gamma signaling in the central nervous system controls the course of experimental autoimmune encephalomyelitis independently of the localization and composition of inflammatory foci. Journal of Neuroi. [PMID: 22248039]
Show All 15 Publications.

Review for MBP Antibody (NB600-717) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Feline.

Reviews using NB600-717:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen MBP NB600-717
Enlarge
reviewed by:
Anonymous
IHC-Fr Feline 12/08/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested brain and spinal cord
SpeciesFeline
Lot161031

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
12/08/2017
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol MBP
Entrez
Uniprot