![Western Blot: HDAC10 Antibody [NB600-223] - WB analysis of HDAC10 in HeLa cells with NB600-223 at a dilution of 1:250.](http://images.novusbio.com/fullsize/HDAC10-Antibody-Western-Blot-NB600-223-img0001.jpg "Western Blot: HDAC10 Antibody [NB600-223] - WB analysis of HDAC10 in HeLa cells with NB600-223 at a dilution of 1:250.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit HDAC10 Antibody - BSA Free (NB600-223) is a polyclonal antibody validated for use in WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Synthetic peptide corresponding to a portion of human HDAC 10. |
| Specificity | Reacts with the human HDAC 10 protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HDAC10 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western blot, a predominant band is seen at ~65-70 kDa; it has been reported that HDAC10 variants seem present in certain cell lines and tissues that are as of yet uncharacterized. Other bands may be seen below 50 kDa, and in some cases they are predominant. Simple Western reported in verified customer review. See Simple Western Antibody Database for Simple Western validation: Tested in Human SVG-A Protein Lysate, separated by Size, antibody dilution of 1:25 |
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| Theoretical MW | 62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry-Paraffin KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0013.jpg)
![Immunohistochemistry-Paraffin KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2350-img0015.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
Simple Western | Human and Rat | 03/08/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for HDAC10 Antibody (NB600-223)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 03/08/2019 |
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| Application: | Simple Western | |
| Species: | Human and Rat |
![Simple Western: HDAC10 Antibody [NB600-223] - 1:25 dilution against the indicated concentration of protein lysate (Human SVG-A). Image from verified customer review.](http://images.novusbio.com/fullsize/HDAC10-Antibody-Simple-Western-NB600-223-img0002.jpg "Simple Western: HDAC10 Antibody [NB600-223] - 1:25 dilution against the indicated concentration of protein lysate (Human SVG-A). Image from verified customer review.")
![Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/CBP_MAB2676_Western_Blot_6043.jpg)
![Immunocytochemistry CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/mab2676_human-mouse-rat-cbp-mab-clone-257003-immunocytochemistry-101220208237.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
