HDAC1 Antibody

Images

 
Genetic Strategies: Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval ...read more
Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

HDAC1 Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 1-5, 433-448 and 467-482 of human HDAC1 (Genbank Accession no. Q13547).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500. Use reported in multiple pieces of scientific literature
  • Immunohistochemistry-Frozen 1:10-1:500. Use reported in scientific literature (PMID 26551542)
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (PMID 25648995)
  • Knockout Validated
  • Simple Western 1:200
  • Western Blot 2 ug/ml
Application Notes
In 293, a 60 kDa band is observed. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
Control
293 Whole Cell Lysate
HDAC1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-56340 in the following applications:

Publications
Read Publications using
NB100-56340 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25648995). Rat reactivity reported in scientific literature (PMID: 26551542)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HDAC1 Antibody

  • EC 3.5.1.98
  • GON-10
  • HD1
  • HD1DKFZp686H12203
  • Histone Deacetylase 1
  • KDAC1
  • reduced potassium dependency, yeast homolog-like 1
  • RPD3L1
  • RPD3L1RPD3

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HDAC1 Antibody (NB100-56340)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, KD, Simple Western, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
KD
(1)
Simple Western
(2)
WB
(10)
All Applications
Filter By Species
Human
(3)
Mouse
(7)
Rat
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-56340 Applications Species
Kappert L, Ruzicka P, Kutikhin A Et al. Loss of Nfat5 promotes lipid accumulation in vascular smooth muscle cells FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-09-01 [PMID: 34383982] (WB, Mouse) WB Mouse
Lazarchuk P, Hernandez-Villanueva J, Pavlova Mn Et Al. Mutual balance of histone deacetylases HDAC1, HDAC2, and the acetyl reader ATAD2 regulates the level of acetylation of histone H4 on nascent chromatin of human cells Mol. Cell. Biol. 2020-02-03 [PMID: 32015101] (WB, KD, ICC/IF) WB, KD, ICC/IF
Laumet G, Garriga J, Chen SR et al. G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat. Neurosci. 2015-12-01 [PMID: 26551542] (WB, IHC-Fr, Rat) WB, IHC-Fr Rat
Zimberlin CD, Lancini C, Sno R et al. HDAC1 and HDAC2 collectively regulate intestinal stem cell homeostasis FASEB J. 2015-02-03 [PMID: 25648995] (IHC-P, Mouse)

Details:
HDAC1 antibody used for IHC-P application on intestine of WT, HOM;HET, and DKO mice, 4 /8 days after stimulation with beta naphthoflavone/b-NF (Figure 1B and 1F). The IHC-P involved - 24 hours tissue fixation in ethanol-acetic acid-formol saline fixative, blocking with goat serum for 30 minutes, overnight incubation with primary antibody, detection with HRP conjugated primary and DAB. The antibody also used for immuno-cytochemistry staining (DAB detection) of DKO mouse organoids subjected to control or beta-NF treatment in vitro for 72 hours (Figure 3B).
IHC-P Mouse
Matthias Patrick. Too much or too little, how much HDAC activity is good for you? Blood. 2013-03-14 [PMID: 23493769] (WB) WB
Johnson AM The Role of the Tumor Microenvironment on the Progression of Lung Cancer Thesis 2020-01-01 (WB, Mouse, Human) WB Mouse, Human
Laban H, Siegmund S, Zappe M et al. NFAT5/TonEBP Limits Pulmonary Vascular Resistance in the Hypoxic Lung by Controlling Mitochondrial Reactive Oxygen Species Generation in Arterial Smooth Muscle Cells Cells 2021-11-24 [PMID: 34943801]
Zhang, Q;Chao, TC;Patil, VS;Qin, Y;Tiwari, SK;Chiou, J;Dobin, A;Tsai, CM;Li, Z;Dang, J;Gupta, S;Urdahl, K;Nizet, V;Gingeras, TR;Gaulton, KJ;Rana, TM; The long noncoding RNA ROCKI regulates inflammatory gene expression EMBO J. 2019-04-15 [PMID: 30918008] (WB, Human) WB Human
Heideman Marinus R, Lancini Cesare, Proost Natalie et al. Sin3a-associated Hdac1 and Hdac2 are essential for hematopoietic stem cell homeostasis and contribute differentially to hematopoiesis. Haematologica. 2014-08-01 [PMID: 24763403] (WB, Mouse) WB Mouse
Heideman MR, Wilting RH, Yanover E et al. Dosage-dependent tumor suppression by histone deacetylases 1 and 2 through regulation of c-Myc collaborating genes and p53 function. Blood. 2013-03-14 [PMID: 23327920]
Show All 18 Publications.

Reviews for HDAC1 Antibody (NB100-56340) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Pig.

Reviews using NB100-56340:
Filter by Applications
WB
(2)
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Mouse
(1)
Pig
(1)
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Images Ratings Applications Species Date Details
Western Blot HDAC1 NB100-56340
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4
reviewed by:
Aileen Keating
WB Pig 11/10/2016
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Summary

ApplicationWestern Blot
Sample TestedOvary tissue
SpeciesPig

Comments

Comments-Blocked in 5% BSA .2% PBST for 4 hours
-Primary was 1:250 in 5% BSA .2% PBST overnight at 4C
-Secondary was goat x rabbit 1:1000 in 5% BSA .2% PBST 1 hour
  5
reviewed by:
Verified Customer
WB Mouse 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23327920
SpeciesMouse

Product General Protocols

View specific protocols for HDAC1 Antibody (NB100-56340): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HDAC1 Antibody (NB100-56340) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional HDAC1 Products

Research Areas for HDAC1 Antibody (NB100-56340)

Find related products by research area.

Blogs on HDAC1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Aileen Keating
11/10/2016
Application: WB
Species: Pig

 
Verified Customer
12/12/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HDAC1
Entrez
Uniprot