HDAC1 Antibody

Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

HDAC1 Antibody Summary

• Immunogen: This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 1-5, 433-448 and 467-482 of human HDAC1 (Genbank Accession no. Q13547).
• Specificity: In 293, a 60 kDa band is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockout Validated
  • Simple Western 1:200
  • Western Blot 2 ug/ml
• Application Notes: Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25648995). Use in Immunohistochemistry reported in multiple pieces of scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26551542).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
• Control:

  293 Whole Cell Lysate
  HDAC1 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25648995). Rat reactivity reported in scientific literature (PMID: 26551542)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HDAC1 Antibody

• EC 3.5.1.98
• GON-10
• HD1
• HD1DKFZp686H12203
• Histone Deacetylase 1
• KDAC1
• reduced potassium dependency, yeast homolog-like 1
• RPD3L1
• RPD3L1RPD3

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HDAC1 Antibody (NB100-56340)(15)

Publications using NB100-56340

• Kappert L, Ruzicka P, Kutikhin A Et al. Loss of Nfat5 promotes lipid accumulation in vascular smooth muscle cells FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-09-01 [PMID: 34383982] (WB, Mouse)
• Wijdeven RH, van Luijn MM, Wierenga-Wolf AF et al. Chemical and genetic control of IFNg-induced MHCII expression. EMBO Rep. 2018-07-18 [PMID: 30021835] (WB, Human)
• Laumet G, Garriga J, Chen SR et al. G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat. Neurosci. 2015-12-01 [PMID: 26551542] (WB, IHC-Fr, Rat)
• Zimberlin CD, Lancini C, Sno R et al. HDAC1 and HDAC2 collectively regulate intestinal stem cell homeostasis FASEB J. 2015-02-03 [PMID: 25648995] (IHC-P, Mouse)
  
  Details:
  HDAC1 antibody used for IHC-P application on intestine of WT, HOM;HET, and DKO mice, 4 /8 days after stimulation with beta naphthoflavone/b-NF (Figure 1B and 1F). The IHC-P involved - 24 hours tissue fixation in ethanol-acetic acid-formol saline fixative, blocking with goat serum for 30 minutes, overnight incubation with primary antibody, detection with HRP conjugated primary and DAB. The antibody also used for immuno-cytochemistry staining (DAB detection) of DKO mouse organoids subjected to control or beta-NF treatment in vitro for 72 hours (Figure 3B).
• Heideman Marinus R, Lancini Cesare, Proost Natalie et al. Sin3a-associated Hdac1 and Hdac2 are essential for hematopoietic stem cell homeostasis and contribute differentially to hematopoiesis. Haematologica. 2014-08-01 [PMID: 24763403] (WB, Mouse)
• Matthias Patrick. Too much or too little, how much HDAC activity is good for you? Blood. 2013-03-14 [PMID: 23493769] (WB)
• Heideman MR, Wilting RH, Yanover E et al. Dosage-dependent tumor suppression by histone deacetylases 1 and 2 through regulation of c-Myc collaborating genes and p53 function. Blood. 2013-03-14 [PMID: 23327920]
• Huang Hui, Dawicki Wojciech, Zhang Xiaobei et al. Tolerogenic dendritic cells induce CD4+CD25hiFoxp3+ regulatory T cell differentiation from CD4+CD25-/loFoxp3- effector T cells. J Immunol. 2010-11-01 [PMID: 20870943] (IF/IHC, Mouse)
• Wilting Roel H, Yanover Eva, Heideman Marinus R et al. Overlapping functions of Hdac1 and Hdac2 in cell cycle regulation and haematopoiesis. EMBO J. 2010-08-04 [PMID: 20571512] (IF/IHC)
• Wilson AJ, Byun DS, Nasser S et al. HDAC4 promotes growth of colon cancer cells via repression of p21. Mol Biol Cell. 2008-10-01 [PMID: 18632985] (WB)
  
  Details:
  Human HTC116 cells: 1. HDAC1 (IMG-337): WB: Figs. 2A,B; 5D. 2. HDAC4 (IMG-338): WB: Figs. 1A, E, F, 4C, 5D, 6A, 7B; ChIP: Figs. 10A,C,D,H.
• Wilson AJ, Byun DS, Popova N et al. Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer. J Biol Chem. 2006-05-12 [PMID: 16533812]
• Vlaming H, McLean C, Korthout T, Alemdehy MF. Evolutionarily-conserved chromatin crosstalk generates a DOT1L-dose dependency in thymic lymphoma caused by loss of HDAC1 bioRxiv 2019-01-10 (WB, Mouse)
• Zhang, Q;Chao, TC;Patil, VS;Qin, Y;Tiwari, SK;Chiou, J;Dobin, A;Tsai, CM;Li, Z;Dang, J;Gupta, S;Urdahl, K;Nizet, V;Gingeras, TR;Gaulton, KJ;Rana, TM; The long noncoding RNA ROCKI regulates inflammatory gene expression EMBO J. 2019-04-15 [PMID: 30918008] (WB, Human)
• Johnson AM The Role of the Tumor Microenvironment on the Progression of Lung Cancer Thesis 2020-01-01 (WB, Mouse, Human)
• Lazarchuk P, Hernandez-Villanueva J, Pavlova Mn Et Al. Mutual balance of histone deacetylases HDAC1, HDAC2, and the acetyl reader ATAD2 regulates the level of acetylation of histone H4 on nascent chromatin of human cells Mol. Cell. Biol. 2020-02-03 [PMID: 32015101] (WB, KD, ICC/IF)

Reviews for HDAC1 Antibody (NB100-56340) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Pig.

reviewed by: Verified Customer WB Pig 11/10/2016

Summary

• Application: Western Blot
• Sample Tested: Ovary tissue
• Species: Pig

Comments

• Comments: -Blocked in 5% BSA .2% PBST for 4 hours
  -Primary was 1:250 in 5% BSA .2% PBST overnight at 4C
  -Secondary was goat x rabbit 1:1000 in 5% BSA .2% PBST 1 hour

reviewed by: cynthia morimoto WB Mouse 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23327920
• Species: Mouse

Bioinformatics

• Gene Symbol: HDAC1
• Entrez:
  • 3065(Human)
• Uniprot:
  • Q01101(Human)