HDAC1 Antibody

Images

 
Western Blot: HDAC1 Antibody [NB100-56340] - analysis of HDAC1 in 293 cell lysate with this antibody.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay ...read more
Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HDAC1 Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 1-5, 433-448 and 467-482 of human HDAC1 (Genbank Accession no. Q13547).
Specificity
In 293, a 60 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:100
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:250
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25648995). Use in Immunohistochemistry reported in multiple pieces of scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26551542).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
293 Lysate (NB800-PC6)
HDAC1 Lysate (NBL1-11477)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-56340 in the following applications:

Publications
Read Publications using
NB100-56340 in the following applications:

Reactivity Notes

Cross reacts with Human. Mouse reactivity reported in scientific literature (PMID: 25648995). Rat reactivity reported in scientific literature (PMID: 26551542)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for HDAC1 Antibody

  • EC 3.5.1.98
  • GON-10
  • HD1DKFZp686H12203
  • histone deacetylase 1
  • reduced potassium dependency, yeast homolog-like 1
  • RPD3L1RPD3

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for HDAC1 Antibody (NB100-56340)(9)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
WB
(4)
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Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-56340 Applications Species
Laumet G, Garriga J, Chen SR et al. G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat. Neurosci. 2015 Dec 01 [PMID: 26551542] (WB, IHC-Fr, Rat) WB, IHC-Fr Rat
Zimberlin CD, Lancini C, Sno R et al. HDAC1 and HDAC2 collectively regulate intestinal stem cell homeostasis FASEB J. 2015 Feb 03 [PMID: 25648995] (IHC-P, Mouse)

Details:
HDAC1 antibody used for IHC-P application on intestine of WT, HOM;HET, and DKO mice, 4 /8 days after stimulation with beta naphthoflavone/b-NF (Figure 1B and 1F). The IHC-P involved - 24 hours tissue fixation in ethanol-acetic acid-formol saline fixative, blocking with goat serum for 30 minutes, overnight incubation with primary antibody, detection with HRP conjugated primary and DAB. The antibody also used for immuno-cytochemistry staining (DAB detection) of DKO mouse organoids subjected to control or beta-NF treatment in vitro for 72 hours (Figure 3B).
IHC-P Mouse
Heideman Marinus R, Lancini Cesare, Proost Natalie et al. Sin3a-associated Hdac1 and Hdac2 are essential for hematopoietic stem cell homeostasis and contribute differentially to hematopoiesis. Haematologica. 2014 Aug [PMID: 24763403] (WB, Mouse) WB Mouse
Matthias Patrick. Too much or too little, how much HDAC activity is good for you? Blood. 2013 Mar 14 [PMID: 23493769] (WB) WB
Heideman MR, Wilting RH, Yanover E et al. Dosage-dependent tumor suppression by histone deacetylases 1 and 2 through regulation of c-Myc collaborating genes and p53 function. Blood. 2013 Mar 14 [PMID: 23327920]
Huang Hui, Dawicki Wojciech, Zhang Xiaobei et al. Tolerogenic dendritic cells induce CD4+CD25hiFoxp3+ regulatory T cell differentiation from CD4+CD25-/loFoxp3- effector T cells. J Immunol. 2010 Nov 1 [PMID: 20870943] (IHC, Mouse) IHC Mouse
Wilting Roel H, Yanover Eva, Heideman Marinus R et al. Overlapping functions of Hdac1 and Hdac2 in cell cycle regulation and haematopoiesis. EMBO J. 2010 Aug 4 [PMID: 20571512] (IHC) IHC
Wilson AJ, Byun DS, Nasser S et al. HDAC4 promotes growth of colon cancer cells via repression of p21. Mol Biol Cell. 2008 Oct [PMID: 18632985] (WB)

Details:
Human HTC116 cells: 1. HDAC1 (IMG-337): WB: Figs. 2A,B; 5D. 2. HDAC4 (IMG-338): WB: Figs. 1A, E, F, 4C, 5D, 6A, 7B; ChIP: Figs. 10 A,C,D,H.
WB
Wilson AJ, Byun DS, Popova N et al. Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer. J Biol Chem. 2006 May 12 [PMID: 16533812]

Reviews for HDAC1 Antibody (NB100-56340) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Pig.
We have 2 review tested in 1 application: WB.

Reviews using NB100-56340:
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WB
(2)
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(1)
Pig
(1)
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Images Ratings Applications Species Date Details
Western Blot HDAC1 NB100-56340
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reviewed by:
Aileen Keating
WB Pig 11/10/2016
View

Summary

ApplicationWestern Blot
Sample TestedOvary tissue
SpeciesPig

Comments

Comments-Blocked in 5% BSA.2% PBST for 4 hours
-Primary was 1:250 in 5% BSA.2% PBST overnight at 4C
-Secondary was goat x rabbit 1:1000 in 5% BSA.2% PBST 1 hour
 
reviewed by:
Anonymous
WB Mouse 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23327920
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23327920

Details

Detection NotesSee PMID 23327920

Comments

CommentsPublished in PMID: 23327920

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HDAC1 Antibody (NB100-56340) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for HDAC1 Antibody (NB100-56340)

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Research Areas for HDAC1 Antibody (NB100-56340)

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Blogs on HDAC1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Aileen Keating
11/10/2016
Application: WB
Species: Pig

 
Anonymous
12/12/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HDAC1
Entrez
Uniprot