HDAC3 Antibody

Images

 
Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.
Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was ...read more
Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-1669] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:50 conc. of HDAC3 antibody on a Bond Rx autostainer (Leica Biosystems). ...read more
Simple Western: HDAC3 Antibody [NB100-1669] - Simple Western lane view shows a specific band for HDAC3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HDAC3 Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 2-17 of human HDAC3.
Specificity
In HeLa, a 50 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 5ug/ml
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
In HeLa, a 50 kDa band is observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HDAC3 Lysate (NBL1-11481)
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB100-1669 in the following applications:

Reactivity Notes

Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) and Chicken (100% identity with immunogen) due to sequence homology. Xenopus (100% identity with immunogen), Feline (100% identity with immunogen), Canine (100% identity with immunogen).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HDAC3 Antibody

  • EC 3.5.1.98
  • HD3RPD3-2RPD3
  • histone deacetylase 3
  • SMAP45

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for HDAC3 Antibody (NB100-1669)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ChIP, ICC/IF, IHC-P, WB.


Filter By Application
ChIP
(2)
ICC/IF
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-1669 Applications Species
Baek MH, Park JY, Rhim CC et al. Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma. Anticancer Res. May 1 2016 [PMID: 27127168] (IHC-P, Human) IHC-P Human
Mano T, Suzuki T, Tsuji S, Iwata A. Differential Effect of HDAC3 on Cytoplasmic and nuclear Huntingtin Aggregates PLoS OnE et al. 2014 Nov 08 [PMID: 25380050] (WB, ICC/IF, Human)

Details:
HDAC3 antibody used for WB on HeLa derived E3 or n3 cells transfected or not with shRnA HDAC3 (Figure 2). Antibody also used for ICC-IF on 4% paraformaldehyd fixed E3 or n3 cells - HDAC3 associates exclusively with nuclear inclusion bodies (Figure 4C)
WB, ICC/IF Human
Wilson AJ, Byun D-S, Nasser S et al. HDAC4 Promotes Growth of Colon Cancer Cells via Repression of p21. Mol Biol Cell;19(10):4062-4075. 2008 [PMID: 18632985]
Jiang Chunling, Zhou Bingsen, Fan Kenneth et al. A sequential treatment of depsipeptide followed by 5-azacytidine enhances Gadd45beta expression in hepatocellular carcinoma cells. Anticancer Res. 2007 Nov-Dec [PMID: 18225533] (WB, Human) WB Human
Wilson, A J, Byun, D-S, Popova, N, Murray, L B, Italien, K, Sowa, Y, Arango, D, Velcich, A, Augenlicht, L H, Mariadason, J M. Histone Deacetylase 3 (HDAC3) Other Class I HDACs Regulate Colon Cell Maturation p21 Expression Are Deregulated in Human Colon Cancer. J Biol Chem281(19):13548-58. 2006 May 12. [PMID: 16533812]
Imbriano C, A Gurtner, F Cocchiarella, S Di Agostino, V Basile, M Gostissa, M Dobbelstein, G Del Sal, G Piaggion, R Mantovani. Direct p53 Transcriptional Repression: In vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol 25:3737-3751. [PMID: 15831478] (ChIP) ChIP
Caretti G, V Salsi, C Vecchi, C Imbriano, R Mantovani. Dynamic recruitment of NF-Y histone acetyltransferases on cell-cycle promoters. J Biol Chem :30435-30440. 2005 [PMID: 12771133] (ChIP)

Details:
ChIP, Fig. 3
ChIP

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol HDAC3
Entrez