HDAC3 Antibody


Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.
Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 preferably binds to nuclear Htt with long Qs. HDAC3 associates exclusively with nuclear inclusion bodies. E3 or N3 cells were fixed and ...read more
Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-1669] - Analysis human colon cancer using 1:50 conc. of HDAC3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced ...read more
Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was ...read more
Simple Western: HDAC3 Antibody [NB100-1669] - Lane view shows a specific band for HDAC3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Reactivity Hu, Mu, Rt, Ca, Ch, Fe, Pm, XpSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

Order Details

HDAC3 Antibody Summary

This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 2-17 of human HDAC3.
In HeLa, a 50 kDa band is observed.
Predicted Species
Mouse (100%), Rat (100%), Chicken (100%), Canine (100%), Xenopus (100%), Primate (100%), Feline (100%). Backed by our 100% Guarantee.
Protein G purified
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  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:25
  • Western Blot 2 - 5ug/ml
Application Notes
In HeLa, a 50 kDa band is observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
HDAC3 Overexpression Lysate
HeLa Whole Cell Lysate
Read Publications using
NB100-1669 in the following applications:

  • 3 publications
  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Immunogen sequence has 100% homology with many species.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for HDAC3 Antibody

  • EC
  • HD3RPD3-2RPD3
  • HDAC3
  • Histone Deacetylase 3
  • KDAC3
  • RPD3-2
  • SMAP45


Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC3 Antibody (NB100-1669)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 9 of 9.
Publications using NB100-1669 Applications Species
Wang J, Liu R, Wang Y Et Al. Repression of the miR-627-5p by histone deacetylase 3 contributes to hypoxia-induced hepatocellular carcinoma progression J Cancer 2021-08-02 [PMID: 34335948] (ChIP) ChIP
Baek, M H, Park, J Y Et al. The combination of histone deacetylase and p53 expressions and histological subtype has prognostic implication in uterine leiomyosarcoma. Jpn J Clin Oncol 2019-08-01 [PMID: 31329907] (IP, Mouse) IP Mouse
Baek MH, Park JY, Rhim CC et al. Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma. Anticancer Res. 2016-05-01 [PMID: 27127168] (IHC-P, Human) IHC-P Human
Mano T, Suzuki T, Tsuji S, Iwata A. Differential Effect of HDAC3 on Cytoplasmic and nuclear Huntingtin Aggregates PLoS OnE et al. 2014-11-08 [PMID: 25380050] (ICC/IF, WB, Human)

HDAC3 antibody used for WB on HeLa derived E3 or n3 cells transfected or not with shRnA HDAC3 (Figure 2). Antibody also used for ICC-IF on 4% paraformaldehyd fixed E3 or n3 cells - HDAC3 associates exclusively with nuclear inclusion bodies (Figure 4C)
ICC/IF, WB Human
Wilson AJ, Byun DS, Nasser S et al. HDAC4 promotes growth of colon cancer cells via repression of p21. Mol Biol Cell. 2008-10-01 [PMID: 18632985]

Human HTC116 cells: 1. HDAC1 (IMG-337): WB: Figs. 2A,B; 5D. 2. HDAC4 (IMG-338): WB: Figs. 1A, E, F, 4C, 5D, 6A, 7B; ChIP: Figs. 10A,C,D,H.
Jiang Chunling, Zhou Bingsen, Fan Kenneth et al. A sequential treatment of depsipeptide followed by 5-azacytidine enhances Gadd45beta expression in hepatocellular carcinoma cells. Anticancer Res. 2007-11-01 [PMID: 18225533] (WB, Human) WB Human
Wilson AJ, Byun DS, Popova N et al. Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer. J Biol Chem. 2006-05-12 [PMID: 16533812]
Imbriano C, Gurtner A, Cocchiarella F et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol. 2005-05-01 [PMID: 15831478] (ChIP) ChIP
Caretti G, Salsi V, Vecchi C et al. Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters. J Biol Chem. 2003-08-15 [PMID: 12771133] (ChIP)

ChIP, Fig. 3

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol HDAC3