Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.
Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 preferably binds to nuclear Htt with long Qs. HDAC3 associates exclusively with nuclear inclusion bodies. E3 or N3 cells were fixed and ...read more
Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-1669] - Analysis human colon cancer using 1:50 conc. of HDAC3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced ...read more
Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was ...read more
Simple Western: HDAC3 Antibody [NB100-1669] - Lane view shows a specific band for HDAC3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunogen sequence has 100% homology with many species.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for HDAC3 Antibody
Histone Deacetylase 3
Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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