E-Cadherin Antibody (7H12)

Images

 
Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - Human kidney distal tubules stained with E-CAD antibody. Secondary antibody: Donkey anti-Mouse Alexa Fluor 555. Imaging with a 40x objective from ...read more
Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon PDK1 knockdown in Colo205 and HT29 cells using E cadherin (NBP2-19051), N ...read more
Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of E-Cadherin in LNCAP (1), A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - ICC staining of E-Cadherin in human colon cancer sphere. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - Human colonic tissue was stained with E-Cadherin antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin antibody in mouse colon cancer tissue. Antibody dilution 1:500. Image from verified customer review.
Flow Cytometry: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of Hela cells using E-Cadherin mouse mAb (green) and negative control (purple).
Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin in gastric cancer tissues (left) and lung cancer tissues (right) with DAB staining.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
7H12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

E-Cadherin Antibody (7H12) Summary

Immunogen
Purified recombinant fragment of human CDH1 expressed in E. Coli.
Marker
Epithelial Cell Marker, Adherens Junctions Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDH1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 20605541).
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate
A-431 Whole Cell Lysate
Reviewed Applications
Read 7 Reviews rated 4.4
using
NBP2-19051 in the following applications:

Publications
Read Publications using
NBP2-19051 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for E-Cadherin Antibody (7H12)

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Background

E-Cadherin is a transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-Cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Tissue specificity: Non-neural epithelial tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for E-Cadherin Antibody (NBP2-19051)(23)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
WB
(11)
All Applications
Filter By Species
Human
(16)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NBP2-19051 Applications Species
Yano K, Choijookhuu N, Ikenoue M et al. Spatiotemporal expression of HMGB2 regulates cell proliferation and hepatocyte size during liver regeneration Scientific reports Jul 13 2022 [PMID: 35831365] (IHC-P, Mouse) IHC-P Mouse
Karim S, Burzangi AS, Ahmad A et al. PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer International journal of molecular sciences Feb 19 2022 [PMID: 35216429] (WB, Human) WB Human
Jhan JH, Hsu WC, Lee YC et al. MicroRNA-375-3p Suppresses Upper Tract Urothelial Carcinoma Cell Migration and Invasion via Targeting Derlin-1 Cancers Feb 10 2022 [PMID: 35205628] (WB, Human) WB Human
Zhang X, Tanwar VS, Jose CC Et al. Transcriptional repression of E-cadherin in nickel-exposed lung epithelial cells mediated by loss of Sp1 binding at the promoter Molecular carcinogenesis Nov 2 2021 [PMID: 34727382]
Lassiter R, Merchen Td, Fang X, Wang Y Protective Role of Kynurenine 3-Monooxygenase in Allograft Rejection and Tubular Injury in Kidney Transplantation Frontiers in immunology Jul 7 2021 [PMID: 34305900] (IHC-P) IHC-P
Song M, Wang C et al. Targeting ubiquitin protein ligase E3 component N-recognin 5 in cancer cells induces a CD8+ T cell mediated immune response. Oncoimmunology May 5 2020 [PMID: 32363114] (WB, Mouse) WB Mouse
Youngblood RL, Sampson JP, Lebioda KR, Shea LD Microporous scaffolds support assembly and differentiation of pancreatic progenitors into beta-cell clusters Acta Biomater Jun 25 2019 [PMID: 31247380] (ICC/IF, Human) ICC/IF Human
Youngblood R Bioengineered Scaffold Microenvironment Promotes Assembly and Differentiation of Stem Cell-Derived Human Organoids Thesis Jan 1 2020 (IHC-Fr) IHC-Fr
Kuma YI, Hosomichi J, Maeda H et al. Intermittent hypoxia induces turbinate mucosal hypertrophy via upregulating the gene expression related to inflammation and EMT in rats Sleep Breath Aug 6 2020 [PMID: 32766939]
Collin de l'Hortet A, Takeishi K, Guzman-Lepe J et al. Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism Cell Metab. Aug 6 2019 [PMID: 31390551]
Show All 23 Publications.

Reviews for E-Cadherin Antibody (NBP2-19051) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-19051:
Filter by Applications
IHC-P
(2)
WB
(2)
IHC
(1)
Flow
(1)
ICC
(1)
All Applications
Filter by Species
Human
(6)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin E-Cadherin NBP2-19051
Enlarge
5
reviewed by:
Abigail Stone
IHC-P Mouse 02/01/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedColon cancer tissue
SpeciesMouse
Lot7H12

Comments

CommentsAntibody dilution 1:500
Immunohistochemistry-Paraffin E-Cadherin NBP2-19051
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 08/08/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney
SpeciesHuman
Lot151127

Comments

CommentsTaken with a 40x Objective from Zeiss Observer microscope with Apotome2
Secondary antibody: Donkey Anti Mouse 555
  4
reviewed by:
Haiquan Lu
WB Human 09/02/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
Immunohistochemistry E-Cadherin NBP2-19051
Enlarge
4
reviewed by:
Verified Customer
IHC Human 11/04/2014
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman colonic tissue
SpeciesHuman
Flow Cytometry E-Cadherin NBP2-19051
Enlarge
3
reviewed by:
Verified Customer
Flow Human 11/04/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman colon cancer cell line HT29
SpeciesHuman
Immunocytochemistry E-Cadherin NBP2-19051
Enlarge
5
reviewed by:
Verified Customer
ICC Human 10/08/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman colon cancer sphere
SpeciesHuman
Western Blot E-Cadherin NBP2-19051
Enlarge
5
reviewed by:
Verified Customer
WB Human 06/13/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for E-Cadherin Antibody (NBP2-19051) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on E-Cadherin.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Carbonic Anhydrase IX Roles in Tumor Growth, Survival and Invasion
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase, strongly induced by hypoxia. CA IX is overexpressed by several cancer cells from many tumor types, and is a component of the pH regulatory system invoked by these cells to comba...  Read full blog post.

E-Cadherin as a Cancer Biomarker
E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and ...  Read full blog post.

E-Cadherin in Cell-Cell Adhesion and Cancer Diagnostics
E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epitheli...  Read full blog post.

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Recent Reviews

4.4
5
4
4
2
3
1
2
0
1
0

Abigail Stone
02/01/2022
Application: IHC-P
Species: Mouse

Verified Customer
08/08/2018
Application: IHC-P
Species: Human

 
Haiquan Lu
09/02/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDH1
Entrez
Uniprot