E-Cadherin Antibody (7H12)

Images

 
Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of E-Cadherin in LNCAP (1), A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - Human colonic tissue was stained with E-Cadherin antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - Human kidney distal tubules stained with E-CAD antibody. Secondary antibody: Donkey anti-Mouse Alexa Fluor 555. Imaging with a 40x objective from ...read more
Flow Cytometry: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of Hela cells using E-Cadherin mouse mAb (green) and negative control (purple).
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - ICC staining of E-Cadherin in human colon cancer sphere. Image from verified customer review.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin in gastric cancer tissues (left) and lung cancer tissues (right) with DAB staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
7H12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

E-Cadherin Antibody (7H12) Summary

Immunogen
Purified recombinant fragment of human CDH1 expressed in E. Coli.
Marker
Epithelial Cell Marker, Adherens Junctions Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDH1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1-2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This E-Cadherin (7H12) antibody is useful for Western blot, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 20605541)
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate
A-431 Whole Cell Lysate
MCF-7 Whole Cell Lysate
E-Cadherin Knockout HeLa Cell Lysate
Reviewed Applications
Read 6 Reviews rated 4.3
using
NBP2-19051 in the following applications:

Publications
Read Publications using
NBP2-19051 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    6 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for E-Cadherin Antibody (7H12)

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Background

E-Cadherin is a transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-Cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Tissue specificity: Non-neural epithelial tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E-Cadherin Antibody (NBP2-19051)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(6)
All Applications
Filter By Species
Human
(11)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-19051 Applications Species
Tanwar, VS;Zhang, X;Jagannathan, L;Jose, CC;Cuddapah, S; Cadmium exposure upregulates SNAIL through miR-30 repression in human lung epithelial cells Toxicol. Appl. Pharmacol. Apr 16 2019 [PMID: 30998937] (WB, Human) WB Human
Ren W, Hou J, Yang C et al. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res. Feb 8 2019 [PMID: 30736829] (WB, Human) WB Human
Qi M, Zhou Y, Liu J et al. AngII induces HepG2 cells to activate epithelial-mesenchymal transition. Exp Ther Med Oct 1 2018 [PMID: 30233697] (IHC, Human) IHC Human
Chuang CH, Wang LY, Wong YM, Lin ES. Anti-metastatic effects of isolinderalactone via the inhibition of MMP-2 and up regulation of NM23-H1 expression in human lung cancer A549 cells. Oncol Lett 2018 Apr [PMID: 29541242] (Human) Human
Jose CC, Jagannathan L, Tanwar VS et al. Nickel exposure induces persistent mesenchymal phenotype in human lung epithelial cells through epigenetic activation of ZEB1. Mol. Carcinog. Mar 12 2018 [PMID: 29528143] (Human) Human
Thamotharan S, Chu A, Kempf K et al. Differential microRNA expression in human placentas of term intra-uterine growth restriction that regulates target genes mediating angiogenesis and amino acid transport. PLoS ONE May 2 2017 [PMID: 28463968] (WB, Human) WB Human
Liao L, Song M, Li X et al. E3 ubiquitin ligase UBR5 drives the growth and metastasis of triple negative breast cancer. Cancer Res. Mar 22 2017 [PMID: 28330927] (WB, Human) WB Human
Agarwalla P, Mukherjee S, Sreedhar B, Banerjee R. Glucocorticoid receptor-mediated delivery of nano gold-withaferin conjugates for reversal of epithelial-to-mesenchymal transition and tumor regression Nanomedicine (Lond) Oct 1 2016 [PMID: 27622735] (WB, Human) WB Human
Mondal SK, Jinka S, Pal K et al. Glucocorticoid Receptor-Targeted Liposomal Codelivery of Lipophilic Drug and Anti-Hsp90 Gene: Strategy to Induce Drug-Sensitivity, EMT-Reversal, and Reduced Malignancy in Aggressive Tumors. Mol Pharm. May 16 2016 [PMID: 27184196] (WB, Human, Mouse) WB Human, Mouse
Cai J, Shen G, Liu S,Meng Q. Downregulation of HMGA2 inhibits cellular proliferation and invasion, improves cellular apoptosis in prostate cancer. Tumour Biol. 2015 Aug 05 [PMID: 26242267] (Human) Human
Show All 11 Publications.

Reviews for E-Cadherin Antibody (NBP2-19051) (6) 4.36

Average Rating: 4.3
(Based on 6 reviews)
We have 6 reviews tested in 1 species: Human.

Reviews using NBP2-19051:
Filter by Applications
IHC-P
(1)
WB
(2)
IHC
(1)
Flow
(1)
ICC
(1)
All Applications
Filter by Species
Human
(6)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin E-Cadherin NBP2-19051
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reviewed by:
David Ivancic
IHC-P Human 08/08/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney
SpeciesHuman
Lot151127

Comments

CommentsTaken with a 40x Objective from Zeiss Observer microscope with Apotome2
Secondary antibody: Donkey Anti Mouse 555
 
reviewed by:
Anonymous
WB Human 09/02/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP

Details

Detection NotesRegular Protocol
Immunohistochemistry E-Cadherin NBP2-19051
Enlarge
reviewed by:
Arun Satelli
IHC Human 11/04/2014
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman colonic tissue
SpeciesHuman
CommentsDetection was sensitive and was similar to that of Cell-signaling antibody.

Blocking

Blocking DetailsFish Gelatin

Primary Anitbody

Dilution Ratio1:200

Secondary Antibody

Secondary Descriptionsecondary FITC

Details

Detection NotesDetection using confocal microscope
Fixation DetailsRegular fixation of IHC paraffin tissue
Wash Description5 min each 3 times

Comments

CommentsDetection was sensitive and was similar to that of Cell-signaling antibody.
Flow Cytometry E-Cadherin NBP2-19051
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reviewed by:
Arun Satelli
Flow Human 11/04/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman colon cancer cell line HT29
SpeciesHuman
CommentsDetection is good for live imaging also, in this case we fixed it and still good levels. No permeabilization here.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:100

Details

Detection NotesDetection using flow cytometer
Fixation DetailsFixing using 4% PFA, Block in 0.25% FBS,
Permeabilization Detailsno permeabilization
Wash Description5 min each 3 times in PBS

Comments

CommentsDetection is good for live imaging also, in this case we fixed it and still good levels. No permeabilization here.
Immunocytochemistry E-Cadherin NBP2-19051
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reviewed by:
Arun Satelli
ICC Human 10/08/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman colon cancer sphere
SpeciesHuman
CommentsAb also tested at 1:2000 works but weak. you can observe borders with 1:1000 very crisp but at 1:2000, they become weak. Also tested by permeabilizing the cells, then it becomes fuzzy. At 4% PFA without permeabilization step, it is much better.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionAnti-mouse Dylight 488 secondary
Secondary Manufacturer Cat#NBP1-75223
Secondary Concentration1:200

Details

Detection NotesDetection using confocal microscope LSM510
Fixation DetailsCells were fixed in 4% PFA, PBS, Overnight 4 deg C
Wash Description5 min each 3 times in PBS

Comments

CommentsAb also tested at 1:2000 works but weak. you can observe borders with 1:1000 very crisp but at 1:2000, they become weak. Also tested by permeabilizing the cells, then it becomes fuzzy. At 4% PFA without permeabilization step, it is much better.
Western Blot E-Cadherin NBP2-19051
Enlarge
reviewed by:
Anony- mous
WB Human 06/13/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from MCF7 cells using E-Cadherin antibody (NBP2-19051, 1:100).
FileView PDF

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for E-Cadherin Antibody (NBP2-19051) (0)

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Secondary Antibodies

 

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Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Carbonic Anhydrase IX Roles in Tumor Growth, Survival and Invasion
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase, strongly induced by hypoxia. CA IX is overexpressed by several cancer cells from many tumor types, and is a component of the pH regulatory system invoked by these cells to comba...  Read full blog post.

E-Cadherin as a Cancer Biomarker
E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and...  Read full blog post.

E-Cadherin in Cell-Cell Adhesion and Cancer Diagnostics
E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epitheli...  Read full blog post.

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Recent Reviews

4.3
5
3
4
2
3
1
2
0
1
0

David Ivancic
08/08/2018
Application: IHC-P
Species: Human

 
Anonymous
09/02/2015
Application: WB
Species: Human

Arun Satelli
11/04/2014
Application: IHC
Species: Human

Bioinformatics

Gene Symbol CDH1
Entrez
Uniprot