E-Cadherin: Novus Biologicals

AF748

E-Cadherin Antibody [Unconjugated]

100 μg

AF748

HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_lightbox">

E-Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

E-Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

E-Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL003) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

NL003) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

E-Cadherin was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E-Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

<b>Biological Strategies Validation. </b>Retention of the spermatogonial phenotype following gene correction.Immunostaining was performed on gene-corrected GT59 (left) and GT65 cells (right): DAZL, a germ cell specific marker; GFRA1, POU5F1, ETV5, CDH1, and SOHLH1, markers of undifferentiated spermatogonia. Additionally, GT59 and GT65 cells were treated with the differentiation factor, retinoic acid (1 µM) or a vehicle control and then immunostained to examine levels of ZBTB16, a marker of undifferentiated spermatogonia. Bar represents 50 microns. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25409432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Localization of MAFB in mouse testes.(A) Localization of MAFB in E18.5 mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), GATA4, or STAR is shown. Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. G; germ cells. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells and Sertoli cells. (B) Localization of MAFB in adult mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), KIT, SCP3, PNA Lectin, or vimentin is shown. Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cellular localization of PEDF in testicular spermatogenic cells. Methanol-fixed cells were used to stain premeiotic and meiotic/postmeiotic cells as described in Figure 2. (A) alpha 6-integrin, (B) GFR-alpha , (C) CDH1, (D) and NC—IF staining without the presence of primary antibody. (E) CREM, (F) Boule, (G) Acrosin and (H) NC—IF staining without the presence of primary antibody. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33498962), licensed under a CC-BY license. Not internally tested by R&D Systems.

Testis morphogenesis of Mafb KO embryos developed normally.(A) Histological section of WT and KO E18.5 testes stained with HE. No morphological alteration or distribution was detectable. (B and C) Counts of Leydig and Sertoli cells from E18.5 WT and KO testes. Embryonic testes (n = 3 per genotype) were sectioned, and 4 sections for each gonad were randomly selected and stained with the germ cell marker E-cadherin (green) together with either STAR (red) or GATA4 (red). Numbers of STAR-positive cells outside seminiferous tubules (Leydig cells) or GATA4-positive cells inside the tubules (Sertoli cells) were counted per unit area. The values are the mean±S.D. *P<0.05. There was no significant difference between WT and KO cell counts. (D) The expression of genes involved in testes development and function. mRNA expression of the gene markers encoding for PGCs (Oct4), Leydig cells (Cyp17a1, StAR, Insl3, Hsd3b1, and Cyp11a1), and Sertoli cells (Amh, Sox9, and WT1) was determined by qRT-PCR in E18.5 WT and Mafb KO testes. Gene expression levels were normalized to Hprt. The bars represent the mean±SEM of five individuals. *P<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.

AF748) at 0.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of mouse intestine were probed for E-Cadherin mRNA (ACD RNAScope Probe, catalog # 408651; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse E-Cadherin polyclonal antibody (R&D Systems catalog # <a class=

AF748) at 0.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells. " class="big_thumb" />

SARS-CoV-2 infection of the OE and brain in hACE2fl mice.(A, B) Immunohistochemistry of SARS-CoV-2 nucleocapsid and epithelial cell E-cadherin in the RE, OE, and OB 2 and 5–6 days after infection of hACE2fl/y and ShhCre/+; hACE2fl/y mice. Arrowheads indicate sites of viral nucleocapsid detection. Representative of N = 4 animals per genotype and time point. Scale bars 100 μm. (C, D) Immunohistochemistry of SARS-CoV-2 nucleocapsid, neuronal NeuN, and glial cell GFAP in the cerebral cortex (Co) 2 and 5–6 days after infection. Arrowheads indicate sites of GFAP+ reactive gliosis. Arrows indicate nucleocapsid colocalization with NeuN staining. Representative of N = 4 animals per genotype and time point. Scale bars 100 μm top, 50 μm bottom. (E) Diagram of the mouse nasal cavity and cranial anatomy. (F) In situ hybridization detection of SARS-CoV-2 mRNA 5 days postinfection reveals virus in the OB and cerebral cortex of the brain, but not the OE of the nose. Scale bar 250 μm. Note: Images in each panel were taken at lower or higher magnification from the same tissue section respective to genotype and highlight different anatomical regions. OB, olfactory bulb of the brain; OE, olfactory epithelium; RE, respiratory epithelium; SARS-CoV-2, Severe Acute Respiratory Syndrome Coronavirus 2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36745682), licensed under a CC-BY license. Not internally tested by R&D Systems.

human intestinal organoid culture protocol. (A) The organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 10x magnification. (B) The organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 20x magnification." class="big_lightbox">

Adult stem cells isolated from human descending colon were cultured following the steps detailed in the <a class=

human intestinal organoid culture protocol. (A) The organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 10x magnification. (B) The organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 20x magnification." class="big_thumb" />

Characterisation of mammary organoids. (A) Endoxifen (endox) mediated knockout of E-cadherin and/or Tp53 in mammary organoids was detected utilizing western blotting. (B) Relative expression of E-cadherin and Tp53 protein in WT, Cdh1−/− and Cdh1−/−Tp53−/− mammary organoids. (C) 20× Brightfield and RFP channel images of mammary organoids induced with endoxifen. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35406381), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF748U-100UG
Alexa Fluor 405	AF748V-100UG
Alexa Fluor 488	AF748G-100UG
Alexa Fluor 532	AF748X-100UG
Alexa Fluor 594	AF748T-100UG
Alexa Fluor 647	FAB748R-025
Alexa Fluor 700	AF748N-100UG
Alexa Fluor 750	AF748S-100UG
Alexa Fluor Plus 405	AF748AFP405-100UG
Alexa Fluor Plus 488	AF748AFP488-100UG
Alexa Fluor Plus 555	AF748AFP555-100UG
Alexa Fluor Plus 594	AF748AFP594-100UG
Alexa Fluor Plus 647	AF748AFP647-100UG
Alexa Fluor Plus 680	AF748AFP680-100UG
Biotin	BAF748

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF748-SP
Carrier Free	FAB748R-025

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AF648

E-Cadherin Antibody [Unconjugated]

(89 Publications)

100 μg

AF648

HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and A431 human epithelial carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for E‑Cadherin at approximately 123 kDa (as indicated) using 5 µg/ml of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a class=NoLineLink href=

042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." class="big_thumb" />

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MCF-7 human breast cancer cell line was stained with Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

E-Cadherin was detected in immersion fixed human epidermoid carcinoma cells using 10 µg/mL Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Human/Mouse SOX2 Monoclonal Antibody (Catalog # <a class=

MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF017). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

HAF017). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_lightbox">

<b>Orthogonal Strategies Validation. </b>E-Cadherin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human stomach probed with ACD RNAScope<sup>®</sup>Probe (Catalog # 311091) and stained using ACD RNAscope<sup>®</sup>2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # <a class=

VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_thumb" />

Impact of H. pylori infection on ES cell-derived gastric organoids. (a) HE staining of ES-cell derived gastric organoids infected with H. pylori for 12 hours. Scale bar: 200 µm. (b) Immunostaining of gastric organoids infected with H. pylori for 12 hours. “No infection” indicates that the organoid was injected with bacteria-free Brucella Broth. HP, H. pylori; E-cad, E-cadherin. Scale bar: 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30374120), licensed under a CC-BY license. Not internally tested by R&D Systems.

Development and Characterization of an iPSC-Derived Fallopian Tube Organoid. (a) Schematic of factors involved in the differentiation of fallopian tube organoids. (b) Bright field image and H&E staining of FTE organoid at day 14. (c–e) Immunocytochemistry for FTE markers TUBB4A, FOXJ1 and PAX8 and epithelial marker CDH1 (E-Cadherin) at organoid culture day 14. (f) Immunocytochemistry for FTE markers PAX8, TUBB4A, OVGP1 and epithelial marker CDH1 at FTE organoid culture day 45, along with human fallopian tube tissue. (g) Immunocytochemistry for FTE markers TUBB4A and PAX8 at FTE organoid culture day 45. (h) Gene expression of fallopian tube markers OVGP1 (for 2 different primers), FOXJ1, TNFaIP2 and PAX8, as well as kidney markers SALL1 and FOXD1 at organoids culture day 45, human fallopian tube and kidney. The color matrix of the heat map represents the log2(Ratio) of each individual gene relative to its expression at the iPSC stage. Relative gene expression to iPSC stage (day 0) was calculated using delta delta Ct method and normalized to endogenous GAPDH level for 87iCTR-n3 iPSC line (i) H&E staining of FTE organoid at culture day 45 and day 180, and human fallopian tube tissue. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28878359), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistological visualization of engineered EcN strains in tissue sections.These sections are taken from proximal colons of mice receiving different bacteria. Sectioning protocol was designed to preserve mucus and luminal content. Sections were stained with fluorescently labeled antibodies: anti-E-cadherin (green), anti-Muc2 (red), and anti-LPS (blue). The first column shows bright-field images of the sections. The last column shows an overlay of all stains. The white dotted lines represent the boundary of the epithelium and mucus layers. The leftmost parts represent the epithelium, the center parts represent the mucus layers and the rightmost parts represent the lumen (scale bar = 100 μm). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31811125), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAB18382) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Human Pro MMP-13 was serially diluted and captured by Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # <a class=

MAB18382) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF648U-100UG
Alexa Fluor 405	AF648V-100UG
Alexa Fluor 488	AF648G-100UG
Alexa Fluor 532	AF648X-100UG
Alexa Fluor 594	AF648T-100UG
Alexa Fluor 647	AF648R-100UG
Alexa Fluor 700	AF648N-100UG
Alexa Fluor 750	AF648S-100UG
Alexa Fluor Plus 405	AF648AFP405-100UG
Alexa Fluor Plus 488	AF648AFP488-100UG
Alexa Fluor Plus 555	AF648AFP555-100UG
Alexa Fluor Plus 594	AF648AFP594-100UG
Alexa Fluor Plus 647	AF648AFP647-100UG
Alexa Fluor Plus 680	AF648AFP680-100UG
Biotin	BAF648

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF648-SP

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(select up to 3 products from this page to compare)

NBP2-19051

E-Cadherin Antibody (7H12)

(7 Reviews)

(29 Publications)

0.1 ml

NBP2-19051

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Novus Catalog # NBP2-19051) at 1:1000 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="E-Cadherin was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Novus Catalog # NBP2-19051) at 1:1000 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # NBP2-19051) at 1:1000 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # NBP2-19051) at 1:1000 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - Human kidney distal tubules stained with E-CAD antibody. Secondary antibody: Donkey anti-Mouse Alexa Fluor 555. Imaging with a 40x objective from Zeiss Observer microscope with Apotome2. Image from verified customer review.

<b>Genetic Strategies Validation. </b>E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0013.jpg

Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of E-Cadherin in LNCAP (1), A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - ICC staining of E-Cadherin in human colon cancer sphere. Image from verified customer review.

<b>Biological Strategies Validation. </b>E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0014.jpg

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - Human colonic tissue was stained with E-Cadherin antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin antibody in mouse colon cancer tissue. Antibody dilution 1:500. Image from verified customer review.

Flow Cytometry: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of Hela cells using E-Cadherin mouse mAb (green) and negative control (purple).

<b>Biological Strategies Validation. </b>E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0015.jpg

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin in gastric cancer tissues (left) and lung cancer tissues (right) with DAB staining.

Mitochondrial fission regulates migration & invasion in BRAFV600E CRC cells through glucose metabolic reprograming. (A) Relative cell invasion post vemurafenib treatment in BRAFV600E cells (n=3, mean ± SEM); statistical analysis was done by unpaired t-test followed by Welch’s correction assuming unequal variance in means, corresponding p values shown. (B) Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAFV600E cells. Relative cellular invasion post (C) DRP1 silencing, (D) PDK1 silencing, & (E) 2-deoxyglucose treatment in Colo205 & HT29 cells (n=3, mean± SEM); statistical analysis was done by unpaired t-test followed by Welch’s correction assuming unequal variance in means, corresponding p values shown. (F) Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon DRP1 silencing, PDK1 knockdown, & 2-DG treatment in Colo205 & HT29 cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB1838

E-Cadherin Antibody (180215) [Unconjugated]

(31 Publications)

100 μg

MAB1838

HAF018). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) at 2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 166 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB1838) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

D492 and D492M cells carry an epithelial and a mesenchymal phenotype, respectively. (A, B) Relative protein levels of phenotype‐specific markers in D492 and D492M cells as measured by simple western immunoassay (A; representative electropherograms, where the x‐axis shows the protein size (kDa), and the y‐axis indicates signal intensity, reflecting the amount of the protein), and the RPPA (B; average ± SD from three technical replicates). (C) Cell growth shown as increase in confluence (y‐axis) during time after seeding (x‐axis) tracked by the Incucyte (average ± SEM, n ≥ 3). (D) D492 and D492M cell colonies formed during 8‐day growth in 3D Matrigel and stained with phalloidin (red) for labeling F‐actin; scale bar, 50 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33759347), licensed under a CC-BY license. Not internally tested by R&D Systems.

Targeting PCa/macrophage AR leads to increased macrophage recruitment and enhanced PCa migration through CCL2 inductionqPCR of CCL2 mRNA in THP-1 scramble (scr) and THP-1 silenced AR (siAR) cells/different PCa cell lines as indicated (left) and qPCR of CCL2 mRNA in C4-2 scr and C4-2 siAR cells (right).qPCR of CCL2 mRNA in THP-1 (scr or siAR) cells co-cultured with C4-2 scr or siAR cells (left) and in C4-2 (scr or siAR) cells co-cultured with THP-1 scr or siAR cells (right).ELISA of CCL2 in 24 h CM of C4-2 scr and C4-2 siAR cells (left) and in 24 h co-cultured CM of C4-2 scr or C4-2 siAR cells/THP-1 scr or siAR cells (right).ELISA of CCL2 in 24 h co-cultured CM of parental LNCaP cells/THP-1 scr or siAR cells (left) and in 24 h co-cultured CM of parental LAPC4 cells/THP-1 scr or siAR cells (right).Migration assay of C4-2 scr and C4-2 siAR cells incubated for 24 h (upper left), parental THP-1 cells/C4-2 scr or siAR cells after co-cultured for 16 h (upper right), parental C4-2 cells/THP-1 scr cells or siAR cells after co-cultured for 24 h (lower left), and C4-2 scr or C4-2 siAR cells/THP-1 scr or siAR cells after co-cultured for 24 h (lower right), (n = 3); bars in graphs (A–E), Mean ± SEM; bars in pictures, 400 μm (magnification 100×).Western blot of CCL2, EMT markers, AR, and PSA in parental C4-2 cells treated with CM of THP-1 scr and siAR, or co-cultured with THP-1 scr and siAR cells for 24 h (left), and in C4-2 scr and siAR cells (right). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23982944), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1838U-100UG
Alexa Fluor 405	FAB1838V-100UG
Alexa Fluor 488	FAB1838G-100UG
Alexa Fluor 532	FAB1838X-100UG
Alexa Fluor 594	FAB1838T-100UG
Alexa Fluor 647	FAB1838R-100UG
Alexa Fluor 700	FAB1838N-100UG
Alexa Fluor 750	FAB1838S-100UG
Alexa Fluor Plus 405	FAB1838AFP405-100UG
Alexa Fluor Plus 488	FAB1838AFP488-100UG
Alexa Fluor Plus 555	FAB1838AFP555-100UG
Alexa Fluor Plus 594	FAB1838AFP594-100UG
Alexa Fluor Plus 647	FAB1838AFP647-100UG
Alexa Fluor Plus 680	FAB1838AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1838-SP
Carrier Free	MAB1838-500

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MAB7481

E-Cadherin Antibody (114420) [Unconjugated]

(18 Publications)

100 μg

MAB7481

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

E-Cadherin was detected in immersion fixed paraffin-embedded sections of mouse Thymus using Rat Anti-Mouse E-Cadherin Monoclonal Antibody (Catalog # MAB7481) at 1ug at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." alt="E-Cadherin was detected in immersion fixed paraffin-embedded sections of mouse Thymus using Rat Anti-Mouse E-Cadherin Monoclonal Antibody (Catalog # MAB7481) at 1ug at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

HAF005). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of P19 mouse embryonal carcinoma cell line and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse E-Cadherin Monoclonal Antibody (Catalog # MAB7481) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

HAF005). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

mlMCD3 cells were stained with Rat Anti-Mouse E‑Cadherin Monoclonal Antibody (Catalog # MAB7481, filled histogram) or isotype control antibody (<a class=

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

E‑Cadherin was detected in perfusion fixed paraffin-embedded sections of mouse colon using Rat Anti-Mouse E‑Cadherin Monoclonal Antibody (Catalog # MAB7481) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (<a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7481U-100UG
Alexa Fluor 405	FAB7481V-100UG
Alexa Fluor 488	FAB7481G-100UG
Alexa Fluor 594	FAB7481T-100UG
Alexa Fluor 647	FAB7481R-100UG
Alexa Fluor 700	FAB7481N-100UG
Alexa Fluor 750	FAB7481S-100UG
Alexa Fluor Plus 405	FAB7481AFP405-100UG
Alexa Fluor Plus 488	FAB7481AFP488-100UG
Alexa Fluor Plus 555	FAB7481AFP555-100UG
Alexa Fluor Plus 594	FAB7481AFP594-100UG
Alexa Fluor Plus 647	FAB7481AFP647-100UG
Alexa Fluor Plus 680	FAB7481AFP680-100UG
Allophycocyanin	FAB7481A-025
Allophycocyanin	FAB7481A-100
Phycoerythrin	FAB7481P-025
Phycoerythrin	FAB7481P-100

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7481-SP
Carrier Free	FAB7481P-100
Carrier Free	FAB7481P-025
Carrier Free	FAB7481A-100
Carrier Free	FAB7481A-025
Carrier Free	FAB7481V-100UG
Carrier Free	FAB7481U-100UG
Carrier Free	FAB7481T-100UG
Carrier Free	FAB7481S-100UG
Carrier Free	FAB7481R-100UG
Carrier Free	FAB7481N-100UG
Carrier Free	FAB7481G-100UG

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NBP2-16258

E-Cadherin Antibody

(3 Publications)

0.1 ml

NBP2-16258

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Mouse cervix. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Rat intestine. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Breast cancer. E-cadherin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Rat prostate. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Mouse intestine. E-Cadherin stained by E-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: E-Cadherin Antibody [NBP2-16258] - MCF-7 whole cell extract A. Control with 3 ug of preimmune Rabbit IgG B. Immunoprecipitation of E-cadherin protein by 3 ug E-cadherin antibody 5 % SDS-PAGE The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Immunoprecipitation: E-Cadherin Antibody [NBP2-16258] - Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 ug of E-Cadherin antibody. Western blot analysis was performed using E-Cadherin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded dog skin.</br>E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. <br>Sample: Paraffin-embedded rat prostate. <br>E-Cadherin antibody (NBP2-16258) diluted at 1:500.<br><br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.</br>Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.</br>Blue: Fluoroshield with DAPI .</br>Scale bar= 10 um.

Western Blot: E-Cadherin Antibody [NBP2-16258] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: E-Cadherin Antibody [NBP2-16258] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.</br>Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.<br>Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: E-Cadherin protein stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. <br>Sample: Paraffin-embedded mouse pancreas. <br>E-Cadherin antibody (NBP2-16258) diluted at 1:400.<br><br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.<br>Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: E-Cadherin protein stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>

Reactivity:

Hu, Mu, Rt, Ca, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ze

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB120-11512

E-Cadherin Antibody (DECMA-1)

(2 Reviews)

(3 Publications)

0.025 ml

NB120-11512

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Cat testis, cat ovary and HEK293 whole cell lysates. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3200 Rat Monoclonal Anti-Uvomorulin/E-Cadherin, Clone: DECMA-1. Detection using Goat Anti-Rat IgG, FITC conjugate.

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 diluted to 1:3200. The antibody was developed using Rabbit Anti-Rat IgG, FITC-conjugate.

Reactivity:

Hu, Mu, Bv, Ca, Fe

Species Glossary

Reactivity: Hu, Mu, Bv, Ca, Fe

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB200-592

pan Cadherin Antibody

(1 Review)

(9 Publications)

0.1 ml

NB200-592

Immunohistochemistry: pan Cadherin Antibody [NB200-592] - Immunoperoxidase staining of a formalin-fixed, paraffin-embedded section of human heart using Rabbit Anti-Pan Cadherin.

Reactivity:

Hu, Mu, Rt, Am, Av, Bv, Ca, Ch, Fi, Rb, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Am, Av, Bv, Ca, Ch, Fi, Rb, Xp

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-53229

E-Cadherin Antibody (4A2)

100 μg

NBP2-53229

Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229] - Staining of human pancreatic tumor. Image from a verified customer review.

Flow Cytometry: E-Cadherin Antibody (4A2) [NBP2-53229] - Flow Cytometric Analysis of human trypsinized MCF-7 cells using E-Cadherin Antibody (4A2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) [NBP2-53229] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (4A2).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54587AF350
Alexa Fluor 405	NBP2-54587AF405
Alexa Fluor 488	NBP2-54587AF488
Alexa Fluor 532	NBP2-54587AF532
Alexa Fluor 594	NBP2-54587AF594
Alexa Fluor 647	NBP2-54587AF647
Alexa Fluor 700	NBP2-54587AF700
Alexa Fluor 750	NBP2-54587AF750
Alexa Fluor Plus 405	NBP2-54587AFP405
Alexa Fluor Plus 488	NBP2-54587AFP488
Alexa Fluor Plus 555	NBP2-54587AFP555
Alexa Fluor Plus 594	NBP2-54587AFP594
Alexa Fluor Plus 647	NBP2-54587AFP647
Alexa Fluor Plus 680	NBP2-54587AFP680
Allophycocyanin	NBP2-54587APC
Allophycocyanin/Cy7	NBP2-54587APCCY7
Biotin	NBP2-54587B
CoraFluor 1	NBP2-54587CL1
DyLight 350	NBP2-54587UV
DyLight 405	NBP2-54587V
DyLight 488	NBP2-54587G
DyLight 550	NBP2-54587R
DyLight 594	NBP2-54587DL594
DyLight 650	NBP2-54587C
DyLight 680	NBP2-54587FR
DyLight 755	NBP2-54587IR
FITC	NBP2-54587F
HRP	NBP2-54587H
Janelia Fluor 525	NBP2-54587JF525
Janelia Fluor 549	NBP2-54587JF549
Janelia Fluor 646	NBP2-54587JF646
Janelia Fluor 669	NBP2-54587JF669
PE	NBP2-54587PE
PE/Atto594	NBP2-54587PEATT594
PE/Cy5.5	NBP2-54587PECY55
PE/Cy7	NBP2-54587PECY7
PerCP	NBP2-54587PCP
mFluor Violet 450 SE	NBP2-54587MFV450
mFluor Violet 500 SE	NBP2-54587MFV500
mFluor Violet 610 SE	NBP2-54587MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54587-100ug

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NBP2-53354

E-Cadherin Antibody (CDH1/1525)

100 μg

NBP2-53354

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin Mouse Monoclonal Antibody (CDH1/1525).

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).

Protein Array: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E-Cadherin Antibody (CDH1/1525).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Human breast cancer tissue stained fro E-cadherin (white) and counterstained with DAPI (blue). Alexa Fluor 750 version of antibody used. Image from verified customer review.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54551AF350
Alexa Fluor 405	NBP2-54551AF405
Alexa Fluor 488	NBP2-54551AF488
Alexa Fluor 532	NBP2-54551AF532
Alexa Fluor 594	NBP2-54551AF594
Alexa Fluor 647	NBP2-54551AF647
Alexa Fluor 700	NBP2-54551AF700
Alexa Fluor 750	NBP2-54551AF750
Alexa Fluor Plus 405	NBP2-54551AFP405
Alexa Fluor Plus 488	NBP2-54551AFP488
Alexa Fluor Plus 555	NBP2-54551AFP555
Alexa Fluor Plus 594	NBP2-54551AFP594
Alexa Fluor Plus 647	NBP2-54551AFP647
Alexa Fluor Plus 680	NBP2-54551AFP680
Allophycocyanin	NBP2-54551APC
Allophycocyanin/Cy7	NBP2-54551APCCY7
Biotin	NBP2-54551B
CoraFluor 1	NBP2-54551CL1
DyLight 350	NBP2-54551UV
DyLight 405	NBP2-54551V
DyLight 488	NBP2-54551G
DyLight 550	NBP2-54551R
DyLight 594	NBP2-54551DL594
DyLight 650	NBP2-54551C
DyLight 680	NBP2-54551FR
DyLight 755	NBP2-54551IR
FITC	NBP2-54551F
HRP	NBP2-54551H
Janelia Fluor 525	NBP2-54551JF525
Janelia Fluor 549	NBP2-54551JF549
Janelia Fluor 646	NBP2-54551JF646
Janelia Fluor 669	NBP2-54551JF669
PE	NBP2-54551PE
PE/Atto594	NBP2-54551PEATT594
PE/Cy5.5	NBP2-54551PECY55
PE/Cy7	NBP2-54551PECY7
PerCP	NBP2-54551PCP
mFluor Violet 450 SE	NBP2-54551MFV450
mFluor Violet 500 SE	NBP2-54551MFV500
mFluor Violet 610 SE	NBP2-54551MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54551-100ug

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MAB18381

E-Cadherin Antibody (180224) [Unconjugated]

(8 Publications)

100 μg

MAB18381

HAF018). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of MCF-7 human breast cancer cell line, BT-20 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, BT-20 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

E-Cadherin was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB18381U-100UG
Alexa Fluor 405	FAB18381V-100UG
Alexa Fluor 488	FAB18381G-025
Alexa Fluor 488	FAB18381G-100
Alexa Fluor 594	FAB18381T-100UG
Alexa Fluor 647	FAB18381R-100UG
Alexa Fluor 700	FAB18381N-100UG
Alexa Fluor 750	FAB18381S-100UG
Alexa Fluor Plus 405	FAB18381AFP405-100UG
Alexa Fluor Plus 488	FAB18381AFP488-100UG
Alexa Fluor Plus 555	FAB18381AFP555-100UG
Alexa Fluor Plus 594	FAB18381AFP594-100UG
Alexa Fluor Plus 647	FAB18381AFP647-100UG
Alexa Fluor Plus 680	FAB18381AFP680-100UG
Allophycocyanin	FAB18381A-025
Allophycocyanin	FAB18381A-100
Biotin	BAM18381
Phycoerythrin	FAB18381P-025
Phycoerythrin	FAB18381P-100

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB18381-SP
Carrier Free	FAB18381P-100
Carrier Free	FAB18381P-025
Carrier Free	FAB18381A-100
Carrier Free	FAB18381A-025
Carrier Free	FAB18381G-100
Carrier Free	FAB18381G-025
Carrier Free	FAB18381V-100UG
Carrier Free	FAB18381U-100UG
Carrier Free	FAB18381T-100UG
Carrier Free	FAB18381S-100UG
Carrier Free	FAB18381R-100UG
Carrier Free	FAB18381N-100UG

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8505-EC

Recombinant Human E-Cadherin Protein, CF

(11 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CDH1 Products By Gene Name

E-Cadherin Antibody [Unconjugated]

E-Cadherin Antibody [Unconjugated]

E-Cadherin Antibody (7H12)

E-Cadherin Antibody (180215) [Unconjugated]

E-Cadherin Antibody (114420) [Unconjugated]

E-Cadherin Antibody

E-Cadherin Antibody (DECMA-1)

pan Cadherin Antibody

E-Cadherin Antibody (4A2)

E-Cadherin Antibody (CDH1/1525)

E-Cadherin Antibody (180224) [Unconjugated]

Recombinant Human E-Cadherin Protein, CF