beta-Catenin Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat ...read more
HeLa human cervical epithelial carcinoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. beta ‑Catenin/DNA complexes were immuno­precipitated using 5 μg ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Catenin at ...read more
beta ‑Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
beta ‑Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at ...read more
HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1329, filled histogram) or control antibody ...read more
beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ChIP, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

beta-Catenin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human beta -Catenin
Ala2-Leu781
Accession # P35222
Specificity
Detects human, mouse and rat beta -Catenin in Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTNNB1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 50 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 2 Reviews rated 4
using
AF1329 in the following applications:

Publications
Read Publications using
AF1329 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Catenin Antibody [Unconjugated]

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Catenin Antibody (AF1329)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(3)
Immunoprecipitation
(1)
Western Blot
(8)
All Applications
Filter By Species
Human
(4)
Mouse
(9)
Rat
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF1329 Applications Species
ZP You, MJ Yu, YL Zhang, K Shi Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll?like receptor?4?NADPH oxidase 4 signaling pathway Mol Med Rep, 2018;0(0):. 2018 [PMID: 30431085] (Western Blot, Mouse) Western Blot Mouse
T Udagawa, Y Harita, K Miura, J Mitsui, KL Ode, S Morishita, S Urae, S Kanda, Y Kajiho, H Tsurumi, HR Ueda, S Tsuji, A Saito, A Oka Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cells Sci Rep, 2018;8(1):2351. 2018 [PMID: 29402915] (IHC-P, Human) IHC-P Human
M Xu, J Horrell, M Snitow, J Cui, H Gochnauer, CM Syrett, S Kallish, JT Seykora, F Liu, D Gaillard, JP Katz, KH Kaestner, B Levin, C Mansfield, JE Douglas, BJ Cowart, M Tordoff, F Liu, X Zhu, LA Barlow, AI Rubin, JA McGrath, EE Morrisey, EY Chu, SE Millar WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation Nat Commun, 2017;8(0):15397. 2017 [PMID: 28589954] (IHC-Fr, Mouse) IHC-Fr Mouse
Dchs1-Fat4 regulation of polarized cell behaviours during skeletal morphogenesis Nat Commun, 2016;7(0):11469. 2016 [PMID: 27145737] (IHC, Mouse) IHC Mouse
Andersson-Sjoland A, Karlsson J, Rydell-Tormanen K ROS-induced endothelial stress contributes to pulmonary fibrosis through pericytes and Wnt signaling. Lab Invest, 2016;96(2):206-17. 2016 [PMID: 26367492] (IHC-P, Mouse) IHC-P Mouse
Maeda A, Ono M, Holmbeck K, Li L, Kilts T, Kram V, Noonan M, Yoshioka Y, McNerny E, Tantillo M, Kohn D, Lyons K, Robey P, Young M WNT1-induced Secreted Protein-1 (WISP1), a Novel Regulator of Bone Turnover and Wnt Signaling. J Biol Chem, 2015;290(22):14004-18. 2015 [PMID: 25864198] (Western Blot, Mouse) Western Blot Mouse
Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R Integrin beta1 controls VE-cadherin localization and blood vessel stability. Nat Commun, 2015;6(0):6429. 2015 [PMID: 25752958] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Tu X, Delgado-Calle J, Condon K, Maycas M, Zhang H, Carlesso N, Taketo M, Burr D, Plotkin L, Bellido T Osteocytes mediate the anabolic actions of canonical Wnt/beta-catenin signaling in bone. Proc Natl Acad Sci U S A, 2015;112(5):E478-86. 2015 [PMID: 25605937] (Western Blot, Mouse) Western Blot Mouse
Reuter S, Martin H, Beckert H, Bros M, Montermann E, Belz C, Heinz A, Ohngemach S, Sahin U, Stassen M, Buhl R, Eshkind L, Taube C The Wnt/beta-catenin pathway attenuates experimental allergic airway disease. J Immunol, 2014;193(2):485-95. 2014 [PMID: 24929002] (IHC-P, Mouse) IHC-P Mouse
Li C, Siragy H High glucose induces podocyte injury via enhanced (pro)renin receptor-Wnt-beta-catenin-snail signaling pathway. PLoS ONE, 2014;9(2):e89233. 2014 [PMID: 24533170] (Western Blot, Mouse) Western Blot Mouse
Show All 15 Publications.

Reviews for beta-Catenin Antibody (AF1329) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using AF1329:
Filter by Applications
IF
(1)
IP
(1)
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Filter by Species
Human
(1)
Rat
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence beta-Catenin AF1329
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reviewed by:
Anonymous
IF Rat 04/03/2015
View

Summary

ApplicationImmunofluorescence
Sample Testedkidney
SpeciesRat
LotHZT0914071

Blocking

Blocking Details1% BSA/PBS, room temperature, 1 hour.

Primary Anitbody

Dilution Ratio10μg/ml antibody in 1%BSA/PBS, 4°C, overnight.

Secondary Antibody

Secondary DescriptionDonkey anti-goat-IgG-R
Secondary Manufacturer Cat#SC-2024
Secondary Concentration1:50

Details

Detection NotesNikon A1 Confocal Microscopes, 500 ms exposure
Fixation DetailsFixation: 4% formaldehyde solution (w/v) with 5% sucrose in PBS, 4°C, overnight.
Wash DescriptionWash 3 times with 1% BSA/PBS for 30 min at room temperature.
 
reviewed by:
Anonymous
IP Human 01/05/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 23474492
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23474492

Details

Detection NotesSee PMID 23474492

Comments

CommentsPublished. See PMID 23474492

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-Catenin Antibody (AF1329) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1329

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Blogs on beta-Catenin.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
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Anonymous
04/03/2015
Application: IF
Species: Rat

 
Anonymous
01/05/2015
Application: IP
Species: Human

Bioinformatics

Gene Symbol CTNNB1
Uniprot