ITK Antibody (HL1264) - Azide and BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% ...read more
Western Blot: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [HL1264] (NBP3-25538) diluted ...read more
Immunohistochemistry-Paraffin: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph ...read more
Immunohistochemistry-Paraffin: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
HL1264
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

ITK Antibody (HL1264) - Azide and BSA Free Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ITK. The exact sequence is proprietary.
Predicted Species
Rat (93%), Bovine (94%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ITK
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for ITK Antibody (HL1264) - Azide and BSA Free

  • EC 2.7.10
  • EC 2.7.10.2
  • EMT
  • EMTMGC126257
  • homolog of mouse T-cell itk/tsk
  • IL2-inducible T-cell kinase
  • ITK
  • Kinase EMT
  • LYK
  • LYKMGC126258
  • PSCTK2
  • T-cell-specific kinase
  • Tcsk
  • tyrosine-protein kinase ITK/TSK
  • Tyrosine-protein kinase Lyk

Background

Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ITK Antibody (NBP3-25538) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for ITK Antibody (NBP3-25538)

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Blogs on ITK

There are no specific blogs for ITK, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol ITK