Catenin alpha 1 Antibody

Images

 
Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Catenin alpha 1 Antibody [NBP2-15723] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. Green: alpha Catenin antibody [N1N3] diluted at 1:200. The signal was developed using goat anti-rabbit IgG ...read more
Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. alpha 1 Catenin antibody [N1N3] dilution: 1:500.
Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse prostate. alpha 1 Catenin antibody [N1N3] dilution: 1:500.
Immunoprecipitation: Catenin alpha 1 Antibody [NBP2-15723] - IP samples: HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of CTNNA1 protein by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Rb, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Catenin alpha 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human alpha 1 Catenin. The exact sequence is proprietary.
Localization
Cytoplasm; cytoskeleton; Cell junction; adherens junction; Cell membrane
Predicted Species
Rhesus Macaque (99%), Bovine (97%), Rabbit (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTNNA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Catenin alpha 1 Antibody

  • Alpha E-catenin
  • alpha-catenin
  • alpha-E-catenin
  • alphaE-catenin
  • cadherin-associated protein
  • CAP102
  • catenin (cadherin-associated protein), alpha 1 (102kD)
  • catenin (cadherin-associated protein), alpha 1, 102kDa
  • catenin alpha-1
  • FLJ36832
  • FLJ52416
  • Renal carcinoma antigen NY-REN-13

Background

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CTNNA1
Uniprot