Slug Antibody (OTI1A6)

Images

 
Western Blot: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLUG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: Slug Antibody (1A6) [NBP2-03886] - Mouse glioma stained with Slug antibody. Image from verified customer review.
Flow Cytometry: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLUG antibody, and then analyzed by ...read more
Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Human Kidney tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] Staining of paraffin-embedded Human lymphoma tissue using anti-SLUG mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Slug Antibody (OTI1A6) Summary

Immunogen
Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SNAI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000
Application Notes
For IHC-P, we recommend using HIER with Tris-EDTA, pH8.0.
Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-03886 in the following applications:

Publications
Read Publications using
NBP2-03886 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Slug Antibody (OTI1A6)

  • MGC10182
  • Neural crest transcription factor Slug
  • Protein snail homolog 2
  • slug homolog, zinc finger protein (chicken)
  • Slug
  • SLUGH
  • SLUGH1
  • SLUGzinc finger protein
  • SNAI2
  • snail 2
  • snail homolog 2 (Drosophila)
  • SNAIL2
  • WS2D
  • zinc finger protein SNAI2

Background

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Slug Antibody (NBP2-03886)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IF/IHC, IHC, IHC-P.


Filter By Application
IF/IHC
(4)
IHC
(3)
IHC-P
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP2-03886 Applications Species
Morris HT, Bamlet WR, Razidlo GL, Machesky LM FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma Pathology, research and practice 2023-10-02 [PMID: 37832352] (IHC, Human)

Details:
Dilution 1:400
IHC Human
Liu L, Deng P, Liu S et al. Enhancer remodeling activates NOTCH3 signaling to confer chemoresistance in advanced nasopharyngeal carcinoma Cell Death & Disease 2023-08-10 [PMID: 37563118] (IHC) IHC
Li SH, Qian L, Chen YH et al. Targeting MYO1B impairs tumorigenesis via inhibiting the SNAI2/cyclin D1 signaling in esophageal squamous cell carcinoma Journal of cellular physiology 2022-07-21 [PMID: 35861939]
Sbiera I, Kircher S, Altieri B et al. Epithelial and Mesenchymal Markers in Adrenocortical Tissues: How Mesenchymal Are Adrenocortical Tissues? Cancers (Basel) 2021-04-30 [PMID: 33917436]
Sad?ecki P, J�?wicki J, Antosik P, Walentowicz-Sad?ecka M. Expression of Selected Epithelial-Mesenchymal Transition Transcription Factors in Endometrial Cancer BioMed Research International 2020-12-29 [PMID: 33457409] (IHC) IHC
Penolazzi L, Bonaccorsi G, Gafa R et al. SLUG/HIF1- alpha/miR-221 regulatory circuit in endometrial cancer Gene 2019-06-18 [PMID: 31220580] (IF/IHC, Human) IF/IHC Human
Sadlecki P, Jozwicki J et al. Expression of selected epithelial-mesenchymal transition transcription factors in serous borderline ovarian tumors and type I ovarian cancers. Tumour Biol 2018-01-06 [PMID: 29952249] (IF/IHC, Human) IF/IHC Human
Stepan A, Ciurea R, Dragoescu P Et Al. Immunoexpression of transcription factors in urothelial bladder carcinomas. Rom J Morphol Embryol. 1905-07-09 [PMID: 29250666] (IF/IHC, Human) IF/IHC Human
Munteanu C, Pirici D, Stepan AE et al. Maxillary calcifying epithelial odontogenic tumor with sinus and buccal vestibule extension: a case report and immunohistochemical study. Diagn Pathol. 2016-11-21 [PMID: 27871286] (IF/IHC, Human) IF/IHC Human
Cappellesso R, Marioni G, Crescenzi M et al. The prognostic role of the Epithelial-Mesenchymal Transition markers E-cadherin and Slug in laryngeal squamous cell carcinoma Histopathology. 2015-02-12 [PMID: 25684546] (IHC-P, Human) IHC-P Human

Review for Slug Antibody (NBP2-03886) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-03886:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Slug NBP2-03886
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 06/06/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse glioma
SpeciesMouse

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FAQs for Slug Antibody (NBP2-03886) (0)

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Recent Reviews

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Verified Customer
06/06/2016
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol SNAI2
Entrez