Slug Antibody (OTI1A6)

Western Blot: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLUG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLUG.

Immunohistochemistry-Paraffin: Slug Antibody (1A6) [NBP2-03886] - Mouse glioma stained with Slug antibody. Image from verified customer review.

Flow Cytometry: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLUG antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SLUG mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SLUG mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SLUG mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SLUG mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Human Kidney tissue using anti-SLUG mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] Staining of paraffin-embedded Human lymphoma tissue using anti-SLUG mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, IHC-P
• Clone: OTI1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Slug Antibody (OTI1A6) Summary

• Immunogen: Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SNAI2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000
• Application Notes: For IHC-P, we recommend using HIER with Tris-EDTA, pH8.0.
• Theoretical MW: 29.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Slug Antibody (OTI1A6)

• MGC10182
• Neural crest transcription factor Slug
• Protein snail homolog 2
• slug homolog, zinc finger protein (chicken)
• Slug
• SLUGH
• SLUGH1
• SLUGzinc finger protein
• SNAI2
• snail 2
• snail homolog 2 (Drosophila)
• SNAIL2
• WS2D
• zinc finger protein SNAI2

Background

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Slug Antibody (NBP2-03886)(7)

Publications using NBP2-03886

• Li SH, Qian L, Chen YH et al. Targeting MYO1B impairs tumorigenesis via inhibiting the SNAI2/cyclin D1 signaling in esophageal squamous cell carcinoma Journal of cellular physiology 2022-07-21 [PMID: 35861939]
• Sbiera I, Kircher S, Altieri B et al. Epithelial and Mesenchymal Markers in Adrenocortical Tissues: How Mesenchymal Are Adrenocortical Tissues? Cancers (Basel) 2021-04-30 [PMID: 33917436]
• Penolazzi L, Bonaccorsi G, Gafa R et al. SLUG/HIF1- alpha/miR-221 regulatory circuit in endometrial cancer Gene 2019-06-18 [PMID: 31220580] (IHC, Human)
• Sadlecki P, Jozwicki J et al. Expression of selected epithelial-mesenchymal transition transcription factors in serous borderline ovarian tumors and type I ovarian cancers. Tumour Biol 2018-01-06 [PMID: 29952249] (IHC, Human)
• Stepan A, Ciurea R, Dragoescu P Et Al. Immunoexpression of transcription factors in urothelial bladder carcinomas. Rom J Morphol Embryol. 1905-07-09 [PMID: 29250666] (IHC, Human)
• Munteanu C, Pirici D, Stepan AE et al. Maxillary calcifying epithelial odontogenic tumor with sinus and buccal vestibule extension: a case report and immunohistochemical study. Diagn Pathol. 2016-11-21 [PMID: 27871286] (IHC, Human)
• Cappellesso R, Marioni G, Crescenzi M et al. The prognostic role of the Epithelial-Mesenchymal Transition markers E-cadherin and Slug in laryngeal squamous cell carcinoma Histopathology. 2015-02-12 [PMID: 25684546] (IHC-P, Human)

Review for Slug Antibody (NBP2-03886) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: JIHYE PAIK IHC-P Mouse 06/06/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse glioma
• Species: Mouse

Bioinformatics

• Gene Symbol: SNAI2
• Entrez:
  • 6591(Human)