N-Cadherin Antibody (13A9)


Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N Cadherin expression in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.
Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
1 mg/ml

N-Cadherin Antibody (13A9) Summary

Cytoplasmic domain of human N Cadherin [Swiss-Prot# P19022]
Cell membrane; Single-pass type I membrane protein.
Mesenchymal Cells Marker
Protein G purified
Innovator's Reward
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1 mg/ml
Protein G purified


Application Notes
This N Cadherin (13A9) antibody is useful for Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band is observed at ~140 kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
140 kDa
Reviewed Applications
Read 1 Review rated 5
NBP1-48309 in the following application:

Read Publications using
NBP1-48309 in the following applications:

  • 1 publication
  • 12 publications
  • 1 publication
  • IP
    4 publications
  • WB
    12 publications

Reactivity Notes

Human and mouse.

Alternate Names for N-Cadherin Antibody (13A9)

  • cadherin 2, N-cadherin (neuronal)
  • cadherin 2, type 1, N-cadherin (neuronal)
  • Cadherin-2
  • CD325 antigen
  • CD325
  • CDH2
  • CDHNcalcium-dependent adhesion protein, neuronal
  • CDw325
  • NCadherin
  • N-cadherin
  • NCADN-cadherin 1
  • Neural cadherin
  • neural-cadherin


N-cadherin (neuronal cadherin; or CDH2/cadherin 2) is a calcium-binding, single pass transmembrane cell adhesion molecule (CAM) that was originally believed to be expressed only by neural cells, but subsequently demonstrated in endothelial cells and pericytes of microvessels, as well as in a variety of poorly differentiated carcinomas. N-cadherin is found in many different types of intercellular junctions, such as adherens junctions and pericyte-endothelial cell junctions, as well as being distributed on the cell surface in non-junctional complexes. It interacts with CDCP1 and forms a part of complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. It also Interact with PCDH8 and TAOK2, and the interaction with PCDH8 leads to internalization through TAOK2/p38 MAPK pathway. N-cadherin promotes the formation of stable intercellular junctions and in addition to mediating cell adhesion, it has several other functions such as activation of FGFR, promotion of vascular smooth muscle and cancer cell migration as well as neurite outgrowth on astrocytes, and contrastingly, inhibits Schwann cell migration on astrocytes. Alongwith vimentin, N-cadherin has emerged as an important marker of EMT in embryonic development and carcinogenic progression.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for N-Cadherin Antibody (NBP1-48309)(20)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: Flow, ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NBP1-48309 Applications Species
Michelhaugh SK, Guastella AR, Varadarajan K et al. Development of patient-derived xenograft models from a spontaneously immortal low-grade meningioma cell line, KCI-MENG1 J Transl Med. 2015 Jul 16 [PMID:26174772] (ICC/IF, Human) ICC/IF Human
Chow AK, Ng L, Lam CS et al. The Enhanced Metastatic Potential of Hepatocellular Carcinoma (HCC) Cells with Sorafenib Resistance. PLoS One. 2013 Nov 11 [PMID:24244338] (WB, Human) WB Human
Maeda M, Johnson E, Mandal SH, Lawson KR, Keim SA, Svoboda RA, Caplan S, Wahl JK 3rd, Wheelock MJ, Johnson KR. Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn). Oncogene25(33):4595-604. 2006 Aug 3. [PMID:16786001] (WB, Human) WB Human
Jaggi M, Nazemi T, Abrahams NA, Baker JJ, Galich A, Smith LM, Balaji KC. N-cadherin switching occurs in high Gleason grade prostate cancer. Prostate66(2):193-9. 2006 Feb 1. [PMID:16173043] (IHC-P, Human) IHC-P Human
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ. N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem278(19):17269-76. 2003 May 9. [PMID:12604612] (WB, IP, ICC/IF, Human) WB, IP, ICC/IF Human
Van Aken EH, Papeleu P, De Potter P, Bruyneel E, Philippe J, Seregard S, Kvanta A, De Laey JJ, Mareel MM. Structure and function of the N-cadherin/catenin complex in retinoblastoma. Invest Ophthalmol Vis Sci43(3):595-602. 2002 Mar. [PMID:11867572] (WB, IP, Flow, ICC/IF, Human) WB, IP, Flow, ICC/IF Human
Reyes-Mugica M, Meyerhardt JA, Rzasa J, Rimm DL, Johnson KR, Wheelock MJ, Reale MA. Truncated DCC reduces N-cadherin/catenin expression and calcium-dependent cell adhesion in neuroblastoma cells. Lab Invest81(2):201-10. 2001 Feb. [PMID:11232642] (WB, ICC/IF, Human) WB, ICC/IF Human
Kim JB, Islam S, Kim YJ, Prudoff RS, Sass KM, Wheelock MJ, Johnson KR. N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility. J Cell Biol151(6):1193-206. 2000 Dec 11. [PMID:11121435] (WB, ICC/IF, Human) WB, ICC/IF Human
Mialhe A, Levacher G, Champelovier P, Martel V, Serres M, Knudsen K, Seigneurin D. Expression of E-, P-, n-cadherins and catenins in human bladder carcinoma cell lines. J Urol164(3 Pt 1):826-35. 2000 Sep. [PMID:10953163]
Nieman MT, Prudoff RS, Johnson KR, Wheelock MJ. N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression. J Cell Biol;147(3):631-44. 1999 Nov 1. [PMID:10545506] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 20 Publications.

Reviews for N-Cadherin Antibody (NBP1-48309) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-48309:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 12/28/2011


ApplicationWestern Blot
Sample TestedHuman
CommentsExcellent antibody


Blocking DetailsBlocking Buffer: 5% non fat milk in PBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: 3 hours, Primary Ab Incubation Temp: room temperature


Detection NotesDetection Method: ECL


CommentsExcellent antibody

Product General Protocols

View specific protocols for N-Cadherin Antibody (NBP1-48309): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for N-Cadherin Antibody (NBP1-48309) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-48309AF405
Alexa Fluor (R) 488 Labeled NBP1-48309AF488
Alexa Fluor (R) 647 Labeled NBP1-48309AF647
Alexa Fluor (R) 700 Labeled NBP1-48309AF700
Allophycocyanin Labeled NBP1-48309APC
Biotin Labeled NBP1-48309B
DyLight 350 Labeled NBP1-48309UV
DyLight 405 Labeled NBP1-48309V
DyLight 405LS Labeled NBP1-48309V3
DyLight 488 Labeled NBP1-48309G
DyLight 550 Labeled NBP1-48309R
DyLight 650 Labeled NBP1-48309C
DyLight 680 Labeled NBP1-48309FR
DyLight 755 Labeled NBP1-48309IR
FITC Labeled NBP1-48309F
HRP Labeled NBP1-48309H
PE Labeled NBP1-48309PE
PerCP Labeled NBP1-48309PCP

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Gene Symbol CDH2

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