Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N Cadherin expression in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.
Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
Protein G purified
Western Blot 0.5 ug/ml
Simple Western 1:50
This N Cadherin (13A9) antibody is useful for Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band is observed at ~140 kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
140 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-48309 in the following applications:
N-cadherin (neuronal cadherin; or CDH2/cadherin 2) is a calcium-binding, single pass transmembrane cell adhesion molecule (CAM) that was originally believed to be expressed only by neural cells, but subsequently demonstrated in endothelial cells and pericytes of microvessels, as well as in a variety of poorly differentiated carcinomas. N-cadherin is found in many different types of intercellular junctions, such as adherens junctions and pericyte-endothelial cell junctions, as well as being distributed on the cell surface in non-junctional complexes. It interacts with CDCP1 and forms a part of complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. It also Interact with PCDH8 and TAOK2, and the interaction with PCDH8 leads to internalization through TAOK2/p38 MAPK pathway. N-cadherin promotes the formation of stable intercellular junctions and in addition to mediating cell adhesion, it has several other functions such as activation of FGFR, promotion of vascular smooth muscle and cancer cell migration as well as neurite outgrowth on astrocytes, and contrastingly, inhibits Schwann cell migration on astrocytes. Alongwith vimentin, N-cadherin has emerged as an important marker of EMT in embryonic development and carcinogenic progression.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.