N-Cadherin Antibody (13A9)


Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N Cadherin expression in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on A549 cells with N-Cadherin Antibody (13A9) NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with ...read more
Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa withN-Cadherin Antibody (13A9) NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with N-Cadherin Antibody (13A9)NBP1-48309R and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with N-Cadherin Antibody (13A9)NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with ...read more
Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP
1 mg/ml

N-Cadherin Antibody (13A9) Summary

Cytoplasmic domain of human N Cadherin [Swiss-Prot# P19022]
Cell membrane; Single-pass type I membrane protein.
Mesenchymal Cells Marker
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5 ug/ml
  • Simple Western 1:50
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
This N Cadherin (13A9) antibody is useful for Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band is observed at approx. 140 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
N-Cadherin Lysate (NBP2-66344)
Reviewed Applications
Read 1 Review rated 5
NBP1-48309 in the following applications:

Read Publications using
NBP1-48309 in the following applications:

  • 1 publication
  • 12 publications
  • IHC
    2 publications
  • 1 publication
  • IP
    4 publications
  • WB
    12 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for N-Cadherin Antibody (13A9)

  • cadherin 2, N-cadherin (neuronal)
  • cadherin 2, type 1, N-cadherin (neuronal)
  • Cadherin-2
  • CD325 antigen
  • CD325
  • CDH2
  • CDHNcalcium-dependent adhesion protein, neuronal
  • CDw325
  • NCadherin
  • N-Cadherin
  • NCADN-cadherin 1
  • Neural cadherin
  • neural-cadherin


N-cadherin (neuronal cadherin; or CDH2/cadherin 2) is a calcium-binding, single pass transmembrane cell adhesion molecule (CAM) that was originally believed to be expressed only by neural cells, but subsequently demonstrated in endothelial cells and pericytes of microvessels, as well as in a variety of poorly differentiated carcinomas. N-cadherin is found in many different types of intercellular junctions, such as adherens junctions and pericyte-endothelial cell junctions, as well as being distributed on the cell surface in non-junctional complexes. It interacts with CDCP1 and forms a part of complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. It also Interact with PCDH8 and TAOK2, and the interaction with PCDH8 leads to internalization through TAOK2/p38 MAPK pathway. N-cadherin promotes the formation of stable intercellular junctions and in addition to mediating cell adhesion, it has several other functions such as activation of FGFR, promotion of vascular smooth muscle and cancer cell migration as well as neurite outgrowth on astrocytes, and contrastingly, inhibits Schwann cell migration on astrocytes. Alongwith vimentin, N-cadherin has emerged as an important marker of EMT in embryonic development and carcinogenic progression.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for N-Cadherin Antibody (NBP1-48309)(23)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NBP1-48309 Applications Species
Chen CY, Melo E, Jakob P et al. N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration. Matrix Biol. Mar 20 2018 [PMID: 29572155] (IHC) IHC
Melo E, Oertle P, Trepp C et al. HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model. EBioMedicine 2017 Dec 13 [PMID: 29269042] (IHC, Human) IHC Human
Koeppen AH, Becker AB, Feustel PJ et al. The significance of intercalated discs in the pathogenesis of Friedreich cardiomyopathy. J Neurol Sci 2016 Aug 15 [PMID: 27423584]
Michelhaugh SK, Guastella AR, Varadarajan K et al. Development of patient-derived xenograft models from a spontaneously immortal low-grade meningioma cell line, KCI-MENG1 J Transl Med. 2015 Jul 16 [PMID: 26174772] (ICC/IF, Human)

N-Cadherin antibody used for Immunocytochemistry on KCI-MENG1 cells or KCI-MENG1-LPSX cells, which are dissociated cells from second generation xenograft mouse tumor) at a dilution of 1:100.
ICC/IF Human
Chow AK, Ng L, Lam CS et al. The Enhanced Metastatic Potential of Hepatocellular Carcinoma (HCC) Cells with Sorafenib Resistance. PLoS One. 2013 Nov 11 [PMID: 24244338] (WB, Human) WB Human
Maeda M, Johnson E, Mandal SH, Lawson KR, Keim SA, Svoboda RA, Caplan S, Wahl JK 3rd, Wheelock MJ, Johnson KR. Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn). Oncogene25(33):4595-604. 2006 Aug 3. [PMID: 16786001] (WB, Human) WB Human
Jaggi M, Nazemi T, Abrahams NA, Baker JJ, Galich A, Smith LM, Balaji KC. N-cadherin switching occurs in high Gleason grade prostate cancer. Prostate66(2):193-9. 2006 Feb 1. [PMID: 16173043] (IHC-P, Human) IHC-P Human
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ. N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem278(19):17269-76. 2003 May 9. [PMID: 12604612] (WB, IP, ICC/IF, Human) WB, IP, ICC/IF Human
Van Aken EH, Papeleu P, De Potter P, Bruyneel E, Philippe J, Seregard S, Kvanta A, De Laey JJ, Mareel MM. Structure and function of the N-cadherin/catenin complex in retinoblastoma. Invest Ophthalmol Vis Sci43(3):595-602. 2002 Mar. [PMID: 11867572] (WB, IP, Flow, ICC/IF, Human) WB, IP, Flow, ICC/IF Human
Reyes-Mugica M, Meyerhardt JA, Rzasa J, Rimm DL, Johnson KR, Wheelock MJ, Reale MA. Truncated DCC reduces N-cadherin/catenin expression and calcium-dependent cell adhesion in neuroblastoma cells. Lab Invest81(2):201-10. 2001 Feb. [PMID: 11232642] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 23 Publications.

Review for N-Cadherin Antibody (NBP1-48309) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-48309:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 12/28/2011


ApplicationWestern Blot
Sample TestedHuman
CommentsExcellent antibody


Blocking DetailsBlocking Buffer: 5% non fat milk in PBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: 3 hours, Primary Ab Incubation Temp: room temperature


Detection NotesDetection Method: ECL


CommentsExcellent antibody

Product General Protocols

View specific protocols for N-Cadherin Antibody (NBP1-48309): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for N-Cadherin Antibody (NBP1-48309) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48309AF405
Alexa Fluor 488 Labeled NBP1-48309AF488
Alexa Fluor 647 Labeled NBP1-48309AF647
Alexa Fluor 700 Labeled NBP1-48309AF700
Biotin Labeled NBP1-48309B
DyLight 350 Labeled NBP1-48309UV
DyLight 405 Labeled NBP1-48309V
DyLight 488 Labeled NBP1-48309G
DyLight 550 Labeled NBP1-48309R
DyLight 650 Labeled NBP1-48309C
DyLight 680 Labeled NBP1-48309FR
DyLight 755 Labeled NBP1-48309IR
FITC Labeled NBP1-48309F
HRP Labeled NBP1-48309H

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Recent Reviews


Application: WB
Species: Human


Gene Symbol CDH2