N-Cadherin Antibody (13A9)

Images

 
Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N Cadherin expression in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa Cells with N-Cadherin Antibody (13A9)NBP1-48309B and a matched isotype control. Both antibodies were conjugated to ...read more
Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa withN-Cadherin Antibody (13A9) NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with N-Cadherin Antibody (13A9)NBP1-48309R and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with N-Cadherin Antibody (13A9)NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on A549 cells with N-Cadherin Antibody (13A9) NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on A431 cells with N-Cadherin Antibody (13A9) NBP1-48309G and a matched isotype control (orange). Cells were fixed with 4% ...read more
Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP
Clone
13A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

N-Cadherin Antibody (13A9) Summary

Immunogen
Cytoplasmic domain of human N Cadherin [Swiss-Prot# P19022]
Localization
Cell membrane; Single-pass type I membrane protein.
Marker
Mesenchymal Cells Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDH2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Simple Western 1:50
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
In Western Blot a band is observed at approx. 140 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
N-Cadherin Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-48309 in the following applications:

Publications
Read Publications using
NBP1-48309 in the following applications:

  • 1 publication
  • 15 publications
  • IHC
    3 publications
  • 1 publication
  • IP
    4 publications
  • WB
    15 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for N-Cadherin Antibody (13A9)

  • cadherin 2, N-cadherin (neuronal)
  • cadherin 2, type 1, N-cadherin (neuronal)
  • Cadherin-2
  • CD325 antigen
  • CD325
  • CDH2
  • CDHNcalcium-dependent adhesion protein, neuronal
  • CDw325
  • NCadherin
  • N-Cadherin
  • NCADN-cadherin 1
  • Neural cadherin
  • neural-cadherin

Background

N-cadherin (neuronal cadherin; or CDH2/cadherin 2) is a calcium-binding, single pass transmembrane cell adhesion molecule (CAM) that was originally believed to be expressed only by neural cells, but subsequently demonstrated in endothelial cells and pericytes of microvessels, as well as in a variety of poorly differentiated carcinomas. N-cadherin is found in many different types of intercellular junctions, such as adherens junctions and pericyte-endothelial cell junctions, as well as being distributed on the cell surface in non-junctional complexes. It interacts with CDCP1 and forms a part of complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. It also Interact with PCDH8 and TAOK2, and the interaction with PCDH8 leads to internalization through TAOK2/p38 MAPK pathway. N-cadherin promotes the formation of stable intercellular junctions and in addition to mediating cell adhesion, it has several other functions such as activation of FGFR, promotion of vascular smooth muscle and cancer cell migration as well as neurite outgrowth on astrocytes, and contrastingly, inhibits Schwann cell migration on astrocytes. Alongwith vimentin, N-cadherin has emerged as an important marker of EMT in embryonic development and carcinogenic progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for N-Cadherin Antibody (NBP1-48309)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
Flow
(1)
ICC/IF
(15)
IHC
(3)
IHC-P
(1)
IP
(4)
WB
(15)
All Applications
Filter By Species
Human
(19)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NBP1-48309 Applications Species
Park S, Kim H, Kim K et al. Sonic hedgehog signalling regulates the self-renewal and proliferation of skin-derived precursor cells in mice Cell Prolif. Aug 27 2018 [PMID: 30151845] (ICC/IF, Mouse) ICC/IF Mouse
Mohammed S, Torres Luquis O, Walls E, Lloyd F. Lymph-Circulating Tumor Cells show distinct properties to Blood-Circulating Tumor Cells and constitute extraordinary efficient metastatic precursors bioRxiv Oct 15 2018 (ICC/IF) ICC/IF
Mohammed S, Torres Luquis O, Walls E, Lloyd F. Lymph-Circulating Tumor Cells show distinct properties to Blood-Circulating Tumor Cells and constitute extraordinary efficient metastatic precursors Acta Biomaterialia Oct 1 2018 [PMID: 31026363] (ICC/IF) ICC/IF
Zhao, K;Li, X;Chen, X;Zhu, Q;Yin, F;Ruan, Q;Xia, J;Niu, Z; Inhibition of miR-140-3p or miR-155-5p by antagomir treatment sensitize chordoma cells to chemotherapy drug treatment by increasing PTEN expression Eur. J. Pharmacol. Apr 10 2019 [PMID: 30980798] (WB, Human) WB Human
Hlavac N, VandeVord PJ. Astrocyte Mechano-Activation by High-Rate Overpressure Involves Alterations in Structural and Junctional Proteins Front Neurol Feb 22 2019 [PMID: 30853931] (WB, Rat) WB Rat
Ren W, Hou J, Yang C et al. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res. Feb 8 2019 [PMID: 30736829] (WB, Human) WB Human
Chiba Y, He B, Yoshizaki K et al. The transcription factor AmeloD stimulates epithelial cell motility essential for tooth morphology J. Biol. Chem. Nov 30 2018 [PMID: 30504223] (IHC, Mouse) IHC Mouse
Chen CY, Melo E, Jakob P et al. N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration. Matrix Biol. Mar 20 2018 [PMID: 29572155] (IHC) IHC
Melo E, Oertle P, Trepp C et al. HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model. EBioMedicine 2017 Dec 13 [PMID: 29269042] (IHC, Human) IHC Human
Koeppen AH, Becker AB, Feustel PJ et al. The significance of intercalated discs in the pathogenesis of Friedreich cardiomyopathy. J Neurol Sci 2016 Aug 15 [PMID: 27423584]
Show All 30 Publications.

Review for N-Cadherin Antibody (NBP1-48309) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-48309:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/28/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsExcellent antibody

Blocking

Blocking DetailsBlocking Buffer: 5% non fat milk in PBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: 3 hours, Primary Ab Incubation Temp: room temperature

Details

Detection NotesDetection Method: ECL

Comments

CommentsExcellent antibody

Product General Protocols

View specific protocols for N-Cadherin Antibody (NBP1-48309): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for N-Cadherin Antibody (NBP1-48309) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48309AF405
Alexa Fluor 488 Labeled NBP1-48309AF488
Alexa Fluor 647 Labeled NBP1-48309AF647
Alexa Fluor 700 Labeled NBP1-48309AF700
Biotin Labeled NBP1-48309B
DyLight 350 Labeled NBP1-48309UV
DyLight 405 Labeled NBP1-48309V
DyLight 488 Labeled NBP1-48309G
DyLight 550 Labeled NBP1-48309R
DyLight 650 Labeled NBP1-48309C
DyLight 680 Labeled NBP1-48309FR
DyLight 755 Labeled NBP1-48309IR
FITC Labeled NBP1-48309F
HRP Labeled NBP1-48309H

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Blogs on N-Cadherin.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

MMP24 (Matrix metalloproteinase-24, matrix metalloproteinase-25, MT5-MMP)
MMP24 is an extracellular matrix (ECM) degradative peptidase enzyme that is a member of the large family of matrix metalloproteinases (MMP). Each MMP has a different substrate specificity, and the aberrant or derailed expression of these is strongly c...  Read full blog post.

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Recent Reviews

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Anonymous
12/28/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDH2
Entrez
Uniprot