N-Cadherin Antibody (13A9)

Images

 
Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N Cadherin expression in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.
Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clone
13A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

N-Cadherin Antibody (13A9) Summary

Immunogen
Cytoplasmic domain of human N Cadherin [Swiss-Prot# P19022]
Localization
Cell membrane; Single-pass type I membrane protein.
Marker
Mesenchymal Cells Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDH2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This N Cadherin (13A9) antibody is useful for Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band is observed at approx. 140 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-48309 in the following applications:

Publications
Read Publications using
NBP1-48309 in the following applications:

  • 1 publication
  • 12 publications
  • 1 publication
  • IP
    4 publications
  • WB
    12 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for N-Cadherin Antibody (13A9)

  • cadherin 2, N-cadherin (neuronal)
  • cadherin 2, type 1, N-cadherin (neuronal)
  • Cadherin-2
  • CD325 antigen
  • CD325
  • CDH2
  • CDHNcalcium-dependent adhesion protein, neuronal
  • CDw325
  • NCadherin
  • N-Cadherin
  • NCADN-cadherin 1
  • Neural cadherin
  • neural-cadherin

Background

N-cadherin (neuronal cadherin; or CDH2/cadherin 2) is a calcium-binding, single pass transmembrane cell adhesion molecule (CAM) that was originally believed to be expressed only by neural cells, but subsequently demonstrated in endothelial cells and pericytes of microvessels, as well as in a variety of poorly differentiated carcinomas. N-cadherin is found in many different types of intercellular junctions, such as adherens junctions and pericyte-endothelial cell junctions, as well as being distributed on the cell surface in non-junctional complexes. It interacts with CDCP1 and forms a part of complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. It also Interact with PCDH8 and TAOK2, and the interaction with PCDH8 leads to internalization through TAOK2/p38 MAPK pathway. N-cadherin promotes the formation of stable intercellular junctions and in addition to mediating cell adhesion, it has several other functions such as activation of FGFR, promotion of vascular smooth muscle and cancer cell migration as well as neurite outgrowth on astrocytes, and contrastingly, inhibits Schwann cell migration on astrocytes. Alongwith vimentin, N-cadherin has emerged as an important marker of EMT in embryonic development and carcinogenic progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for N-Cadherin Antibody (NBP1-48309)(21)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: Flow, ICC/IF, IHC-P, IP, WB.


Filter By Application
Flow
(1)
ICC/IF
(12)
IHC-P
(1)
IP
(4)
WB
(12)
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Human
(16)
Rat
(1)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NBP1-48309 Applications Species
Koeppen AH, Becker AB, Feustel PJ et al. The significance of intercalated discs in the pathogenesis of Friedreich cardiomyopathy. J Neurol Sci 2016 Aug 15 [PMID: 27423584]
Michelhaugh SK, Guastella AR, Varadarajan K et al. Development of patient-derived xenograft models from a spontaneously immortal low-grade meningioma cell line, KCI-MENG1 J Transl Med. 2015 Jul 16 [PMID: 26174772] (ICC/IF, Human)

Details:
N-Cadherin antibody used for Immunocytochemistry on KCI-MENG1 cells or KCI-MENG1-LPSX cells, which are dissociated cells from second generation xenograft mouse tumor) at a dilution of 1:100.
ICC/IF Human
Chow AK, Ng L, Lam CS et al. The Enhanced Metastatic Potential of Hepatocellular Carcinoma (HCC) Cells with Sorafenib Resistance. PLoS One. 2013 Nov 11 [PMID: 24244338] (WB, Human) WB Human
Maeda M, Johnson E, Mandal SH, Lawson KR, Keim SA, Svoboda RA, Caplan S, Wahl JK 3rd, Wheelock MJ, Johnson KR. Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn). Oncogene25(33):4595-604. 2006 Aug 3. [PMID: 16786001] (WB, Human) WB Human
Jaggi M, Nazemi T, Abrahams NA, Baker JJ, Galich A, Smith LM, Balaji KC. N-cadherin switching occurs in high Gleason grade prostate cancer. Prostate66(2):193-9. 2006 Feb 1. [PMID: 16173043] (IHC-P, Human) IHC-P Human
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ. N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem278(19):17269-76. 2003 May 9. [PMID: 12604612] (WB, IP, ICC/IF, Human) WB, IP, ICC/IF Human
Van Aken EH, Papeleu P, De Potter P, Bruyneel E, Philippe J, Seregard S, Kvanta A, De Laey JJ, Mareel MM. Structure and function of the N-cadherin/catenin complex in retinoblastoma. Invest Ophthalmol Vis Sci43(3):595-602. 2002 Mar. [PMID: 11867572] (WB, IP, Flow, ICC/IF, Human) WB, IP, Flow, ICC/IF Human
Reyes-Mugica M, Meyerhardt JA, Rzasa J, Rimm DL, Johnson KR, Wheelock MJ, Reale MA. Truncated DCC reduces N-cadherin/catenin expression and calcium-dependent cell adhesion in neuroblastoma cells. Lab Invest81(2):201-10. 2001 Feb. [PMID: 11232642] (WB, ICC/IF, Human) WB, ICC/IF Human
Kim JB, Islam S, Kim YJ, Prudoff RS, Sass KM, Wheelock MJ, Johnson KR. N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility. J Cell Biol151(6):1193-206. 2000 Dec 11. [PMID: 11121435] (WB, ICC/IF, Human) WB, ICC/IF Human
Mialhe A, Levacher G, Champelovier P, Martel V, Serres M, Knudsen K, Seigneurin D. Expression of E-, P-, n-cadherins and catenins in human bladder carcinoma cell lines. J Urol164(3 Pt 1):826-35. 2000 Sep. [PMID: 10953163]
Show All 21 Publications.

Review for N-Cadherin Antibody (NBP1-48309) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-48309:
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/28/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsExcellent antibody

Blocking

Blocking DetailsBlocking Buffer: 5% non fat milk in PBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: 3 hours, Primary Ab Incubation Temp: room temperature

Details

Detection NotesDetection Method: ECL

Comments

CommentsExcellent antibody

Product General Protocols

View specific protocols for N-Cadherin Antibody (NBP1-48309): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for N-Cadherin Antibody (NBP1-48309) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48309AF405
Alexa Fluor 488 Labeled NBP1-48309AF488
Alexa Fluor 647 Labeled NBP1-48309AF647
Alexa Fluor 700 Labeled NBP1-48309AF700
Biotin Labeled NBP1-48309B
DyLight 350 Labeled NBP1-48309UV
DyLight 405 Labeled NBP1-48309V
DyLight 488 Labeled NBP1-48309G
DyLight 550 Labeled NBP1-48309R
DyLight 650 Labeled NBP1-48309C
DyLight 680 Labeled NBP1-48309FR
DyLight 755 Labeled NBP1-48309IR
FITC Labeled NBP1-48309F
HRP Labeled NBP1-48309H

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Recent Reviews

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Anonymous
12/28/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDH2
Entrez
Uniprot