Immunocytochemistry/ Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.
Flow Cytometry: APC Antibody [NB100-91662] - An intracellular stain was performed on HeLa cells with APC Antibody NB100-91662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Western Blot: APC Antibody [NB100-91662] - Extracts from COLO205 cells.
Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - Paraffin-embedded human colon carcinoma tissue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
310 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-91662 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for APC Antibody - BSA Free
adenomatosis polyposis coli tumor suppressor
adenomatosis polyposis coli
adenomatous polyposis coli protein
adenomatous polyposis coli
Deleted in polyposis 2.5
The adenomatous polyposis syndromes, familial adenomatous polyposis(FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2843 amino acids in length with a molecular weight of 300 kDa, which is frequently mutated in patients suffering from FAP and GS. APC has recently been found to be associated with structural components of intracellular junctions. beta-catenin and gamma-catenin (also called plakoglobin) are involved in the regulation of cellular adhesion. APC and E cadherin compete for binding to specific internal regions of both beta- and gamma-catenin. Interactions between the cytoskeleton and the APC, E cadherin and beta/gamma catenin complex are mediated by alpha-catenin. Like APC, APC2 contains SAMP domains, which are required for conductin binding. Both APC and APC2 regulate the formation of active beta-catenin-Tcf complexes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for APC Antibody (NB100-91662). (Showing 1 - 1 of 1 FAQs).
Do you offer cat# NB100-91662 and NB100-1869AF488 labelled with Cy5 or APC ?
NB100-1869 is validated for Flow, both types of IHC (frozen and paraffin), and ICC/IF ... and already has several conjugates available.We could add this antibody conjugated to APC or Cy5 into our system if interested. NB100-91662 is not validated for Flow but has been validated for IHC (parrafin only IHC-P) ... and already has several conjugates available. We could add both conjugates, but if these are going to be used for Flow it would not be guaranteed.
Beta-catenin - I am versatile! Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl... Read full blog post.
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