Collagen I alpha 1 Antibody - Azide and BSA Free

Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Immunohistochemical staining targeting Collagen I alpha 1 revealed severe disorganization of the annulus fibrosus (AF), and a decrease in staining intensity for group D. On the contrary, the arcshaped and layered AF structure with immunoreactivity for type I collagen staining were mostly maintained in group S when compared to group C. Presented images show the AF at site of surgery, defect, and transplant. Abbreviations: D: AF defect only group, S: AF defect treated with sheet transplantation group, C: sham group, NC: negative control of sample stained without primary antibody (scale bar=300um). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31463464/) licensed under a CC-BY license.

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.

Simple Western: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - TNC deficiency reduced kidney fibrosis in animal models.Deletion of TNC (TNC−/−) significantly attenuated the induction of collagen I assessed by IF following UUO by approximate 30% at day 7 & 10 compared with their wild-type littermates (A, n = 4 for each time point, two-way ANOVA p < 0.05). Consistently, the expression of fibrosis markers, such as collagen I alpha , fibronectin & plasminogen activator inhibitor-1 (PAI-1), were significantly lower in TNC−/− mice at UUO day 7 & 10 (B, n = 4 for each time point, two-way ANOVA, p < 0.05). Western blot showed that the proteins of collagen I alpha & alpha -SMA were also significantly reduced in TNC−/− mice at UUO day 7 (C, n = 7, p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36522320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Am, Av, Ma, Sh, Bv, Ca, Ch, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Vo, Xp
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Collagen I alpha 1 Antibody - Azide and BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: This Collagen I alpha 1 antibody was raised against synthetic peptide corresponding to amino acid residues within the C-terminal telo peptide portion (aa1193-1218) of the human COL1A1, conjugated to KLH. Accession # P02452
• Specificity: This Collagen I alpha 1 antibody is specific for the ~ 140 kDa telopeptide portion of the collagen I alpha 1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
• Predicted Species: Primate (100%), Equine (100%), Vole (100%), Xenopus (100%), Canine (100%), Goat (100%), Hamster (100%), Chicken (100%), Feline (100%), Bovine (100%), Guinea Pig (100%), Rabbit (100%), Finch (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL1A1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:100
  • Western Blot 1:1000
• Application Notes: Use in Immunohistochemistry-Frozen sections was reported in the scientific literature (PMID: 21939397), IHC reactivity reported in scientific literature (PMID: 26045736). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26651081).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Lung and Human Kidney lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Based on the homology of the immunogen, this antibody is expected to recognize the collagen I alpha1 polypeptide in all mammals, birds, and amphibians.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Collagen I alpha 1 Antibody - Azide and BSA Free

• Alpha-1 type I collagen
• COL1A1
• COL-IA1
• Collagen 1 alpha 1
• Collagen 1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• Collagen I alpha 1
• collagen of skin, tendon and bone, alpha-1 chain
• Collagen Type I Alpha 1 Chain
• collagen, type I, alpha 1
• Collagen1
• Collagen-1
• EDSARTH1
• OI4
• pro-alpha-1 collagen type 1
• Type I Procollagen Alpha 1 Chain

Background

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are highly conserved and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. The extensive family of collagens is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagen type I (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues. It is synthesized by fibroblasts, osteoblasts, and odontoblasts and has a theoretical molecular weight of 138 kDa.

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon tissue. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue. Several collagens play a role in cell adhesion, responsible for maintaining normal tissue architecture and function. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Post-Golgi LH3 trafficking is essential for collagen homeostasis and for the development and function of multiple organs and tissues (1).

The COL1A1 gene encodes the pro-alpha1 chains of type I collagen protein, whose triple helix is comprised of two alpha1 chains and one alpha2 chain. Mutations in the encoding COL1A1 gene are associated with brittle bone disease (Osteogenesis Imperfecta), cortical hyperostosis (Caffey disease) and disorders that affect the connective tissues (Ehlers-Danlos syndrome) (2). Studies have found that HIF-1 transcription regulation of collagen prolyl hydroxylases regulates collagen deposition, promoting cancer cell alignment along collagen fibers, which enhances invasion and metastasis to lymph nodes and lung tissue by breast cancer cells (3).

References

1. Banushi, B., Forneris, F., Straatman-Iwanowska, A., Strange, A., Lyne, A. M., Rogerson, C., . . . Gissen, P. (2016). Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nat Commun, 7, 12111. doi:10.1038/ncomms12111

2. Lu, Y., Zhang, S., Wang, Y., Ren, X., & Han, J. (2019). Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen. Intractable Rare Dis Res, 8(2), 98-107. doi:10.5582/irdr.2019.01064

3. Gilkes, D. M., Chaturvedi, P., Bajpai, S., Wong, C. C., Wei, H., Pitcairn, S., . . . Semenza, G. L. (2013). Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res, 73(11), 3285-3296. doi:10.1158/0008-5472.Can-12-3963

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen I alpha 1 Antibody (NBP1-30054)(81)

Publications using NBP1-30054

• Zhang H, Thai PN, Shivnaraine RV, Ren L et Al. Multiscale drug screening for cardiac fibrosis identifies MD2 as a therapeutic target Cell 2024-10-16 [PMID: 39413786]
• Jinsol Han, Chanbin Lee, Hayeong Jeong, Seunghee Jeon, Myunggyo Lee, Haeseung Lee, Yung Hyun Choi, Youngmi Jung Tumor necrosis factor-inducible gene 6 protein and its derived peptide ameliorate liver fibrosis by repressing CD44 activation in mice with alcohol-related liver disease Journal of Biomedical Science 2024-05-24 [PMID: 38790021]
• Qixin Wang, Chiara Goracci, Isaac Kirubakaran Sundar, Irfan Rahman Environmental tobacco smoke exposure exaggerates bleomycin-induced collagen overexpression during pulmonary fibrogenesis Journal of Inflammation (London, England) 2024-03-20 [PMID: 38509574]
• Takashi UMEHARA, Marino OGASAHARA, D.M.V. Supun PREMARATHNE, Yuka SASAKAWA, Yasuo SUMIDA, Masayuki SHIMADA Effect of globin peptide on female fertility in aging granulosa cell-specific Nrg1 knockout mice The Journal of Reproduction and Development 2024-03-12 [PMID: 38479855]
• Bitterli T, Schmid D, Ettinger L et al. Targeted screening of inflammatory mediators in spontaneous degenerative disc disease in dogs reveals an upregulation of the tumor necrosis superfamily JOR SPINE 2023-11-23 [PMID: 38222814] (WB, Canine)
• Zahra Shabani, Joana Schuerger, Xiaonan Zhu, Chaoliang Tang, Li Ma, Alka Yadav, Rich Liang, Kelly Press, Shantel Weinsheimer, Annika Schmidt, Calvin Wang, Abinav Sekhar, Jeffrey Nelson, Helen Kim, Hua Su Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse. Cells 2024-01-12 [PMID: 38201296]
• Wang, Q;Lucas, JH;Pang, C;Zhao, R;Rahman, I; Tobacco and menthol flavored nicotine-free electronic cigarettes induced inflammation and dysregulated repair in lung fibroblast and epithelium Respiratory research 2024-01-10 [PMID: 38200492]
• Livingston MJ, Zhang M, Kwon SH et al. Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair Autophagy 2023-11-18 [PMID: 37978868] (Western Blot)
• Wang Q, Goracci C, Sundar IK, Rahman I Environmental tobacco smoke exposure exaggerates bleomycin- induced collagen overexpression during pulmonary fibrogenesis Research square 2023-10-10 [PMID: 37886473] (WB, IHC, Mouse)
• Huang SS, Liu R, Chang S et al. Gut Microbiota-Derived Tryptophan Metabolite Indole-3-aldehyde Ameliorates Aortic Dissection Nutrients 2023-09-26 [PMID: 37836434] (Immunohistochemistry, Mouse)
• Lee C, Pratap K, Zhang L et al. Inhibition of Wnt/?-catenin signaling reduces renal fibrosis in murine glycogen storage disease type Ia Biochimica et biophysica acta. Molecular basis of disease 2023-09-04 [PMID: 37666439] (IHC-P, Mouse)
• Chiang YP, Li Z, He M et al. Sphingomyelin synthase-related protein SMSr is a phosphatidylethanolamine phospholipase C that promotes nonalcoholic fatty liver disease Journal of Biological Chemistry 2023-08-14 [PMID: 37586586] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Yamanaka Y, Tajima T, Tsujimura Y et al. Adiponectin inhibits fibrosis of the palmar aponeurosis in Dupuytren's contracture in male patients Bone & Joint Research 2023-08-04 [PMID: 37536684] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Wang Q, Lucas JH, Pang C et al. Tobacco and Menthol flavored electronic cigarettes induced inflammation and dysregulated repair in lung fibroblast and epithelium Research square 2023-06-12 [PMID: 37398084] (WB, ICC/IF, Human)
• Kamakura T, Jin Y, Nishio M et al. Collagen X is dispensable for hypertrophic differentiation and endochondral ossification of human iPSC derived chondrocytes JBMR Plus 2023-02-21 [PMID: 37197316] (Simple Western)
• Ichijo R, Maki K, Kabata M et al. Vasculature atrophy causes a stiffened microenvironment that augments epidermal stem cell differentiation in aged skin Nature Aging 2022-07-01 [PMID: 37117774] (IHC-Fr, Mouse)
• Hu X, Ma Z, Li S et al. Fibroblast Growth Factor 2 Is Produced By Renal Tubular Cells to Act as a Paracrine Factor in Maladaptive Kidney Repair After Cisplatin Nephrotoxicity Laboratory Investigation 2023-03-01 [PMID: 36925200] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Li S, Livingston MJ, Ma Z et al. Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity JCI insight 2023-03-14 [PMID: 36917180] (IHC-P, WB, Mouse)
• Wang Q, Sundar IK, Lucas JH et al. Circadian clock molecule REV-ERB? regulates lung fibrotic progression through collagen stabilization Nature communications 2023-03-09 [PMID: 36894533] (Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Mouse)
• Tiskratok W, Yamada M, Watanabe J et al. Substrate stiffness controls proinflammatory responses in human gingival fibroblasts Scientific Reports 2023-01-24 [PMID: 36693942] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• DeDreu J, Le PM, Menko AS The ciliary zonules provide a pathway for immune cells to populate the avascular lens during eye development Experimental biology and medicine (Maywood, N.J.) 2022-12-01 [PMID: 36633170] (IHC-Fr, Avian)
• Li Q, Liu Y, Meng J et al. Role of AIM2 Gene Knockdown Mechanism in Diabetic Cardiomyopathy: an In Vivo and Ex Vivo Study Applied biochemistry and biotechnology 2023-06-01 [PMID: 36622632]
• Wang S, Liu A, Su Y, Dong Z Deficiency of the planar cell polarity protein intu delays kidney repair and suppresses renal fibrosis after acute kidney injury The American journal of pathology 2022-12-28 [PMID: 36586478]
• Xie Q, Zhang M, Mao X et al. Matrix protein Tenascin-C promotes kidney fibrosis via STAT3 activation in response to tubular injury Cell Death & Disease 2022-12-15 [PMID: 36522320] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Liu R, Huang SS, Shi H et al. Alpha-lipoic acid protects against aortic aneurysm and dissection by improving vascular smooth muscle cell function Life sciences 2022-12-15 [PMID: 36368416] (IHC-Fr, WB, Mouse)
• Olmsted ZT, Paluh JL. A combined human gastruloid model of cardiogenesis and neurogenesis iScience 2022-06-17 [PMID: 35721464] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Palladini G, Cagna M, Di Pasqua LG et al. Obeticholic Acid Reduces Kidney Matrix Metalloproteinase Activation Following Partial Hepatic Ischemia/Reperfusion Injury in Rats Pharmaceuticals (Basel) 2022-04-24 [PMID: 35631351] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Livingston MJ, Shu S, Fan Y et al. Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis Autophagy 2022-05-18 [PMID: 35491858] (WB, IHC-P, Human, Mouse)
• Ryu S, Shin JW, Kwon S et al. Siglec-F-expressing neutrophils are essential for creating a pro-fibrotic microenvironment in the renal fibrosis The Journal of clinical investigation [PMID: 35482420] (WB, Human)
• Bai PY, Chen SQ, Jia DL et al. Environmental eustress improves postinfarction cardiac repair via enhancing cardiac macrophage survival Science advances 2022-04-29 [PMID: 35476440] (WB, IHC-P, Mouse)
• Zhuang R, Chen J, Cheng HS et al. Perivascular Fibrosis Is Mediated by a KLF10-IL-9 Signaling Axis in CD4+ T Cells Circulation Research 2022-05-27 [PMID: 35440172] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Rong W, Liu C, Li X et al. Caspase-8 Promotes Pulmonary Hypertension by Activating Macrophage-Associated Inflammation and IL-1 beta (Interleukin 1 beta) Production Arteriosclerosis, thrombosis, and vascular biology 2022-05-01 [PMID: 35387479] (WB, Mouse)
• C Lee, J Kim, J Han, D Oh, M Kim, H Jeong, TJ Kim, SW Kim, JN Kim, YS Seo, A Suzuki, JH Kim, Y Jung Formyl peptide receptor 2 determines sex-specific differences in the progression of nonalcoholic fatty liver disease and steatohepatitis Nature Communications, 2022-01-31;13(1):578. 2022-01-31 [PMID: 35102146] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Ma Z, Hu X, Ding HF et al. Single-nucleus transcriptional profiling of chronic kidney disease after cisplatin nephrotoxicity The American journal of pathology 2022-01-26 [PMID: 35092726] (WB, Mouse)
• Li Z, Chiang Y, He M Et al. Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation iScience 2021-12-01 [PMID: 34927020] (WB, Mouse)
• Wang Q, Chen Z, Peng X et al. Neuraminidase 1 Exacerbating Aortic Dissection by Governing a Pro-Inflammatory Program in Macrophages Frontiers in Cardiovascular Medicine 2021-11-18 [PMID: 34869700] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Basta MD, Paulson H, Walker JL The local wound environment is a key determinant of the outcome of TGF beta signaling on the fibrotic response of CD44+ leader cells in an ex vivo post-cataract-surgery model Experimental eye research 2021-11-10 [PMID: 34774488] (ICC/IF)
• S Dey, LM Udari, P RiveraHern, JJ Kwon, B Willis, JJ Easler, EL Fogel, S Pandol, J Kota Loss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis JCI Insight, 2021-10-08;0(0):. 2021-10-08 [PMID: 34464354] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Su Y, Han W, Kovacs-Kasa A Et Al. HDAC6 Activates ERK in Airway and Pulmonary Vascular Remodeling of COPD American journal of respiratory cell and molecular biology 2021-07-19 [PMID: 34280336]
• ZT Olmsted, JL Paluh Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids Nature Communications, 2021-05-21;12(1):3020. 2021-05-21 [PMID: 34021144]
• Qixin Wang, Isaac K. Sundar, Joseph H. Lucas, Thivanka Muthumalage, Irfan Rahman Molecular clock REV-ERB alpha regulates cigarette smoke–induced pulmonary inflammation and epithelial-mesenchymal transition JCI Insight 2021-06-22 [PMID: 34014841]
• Ji S, Gumina D, McPeak K et al. Human placental villous stromal extracellular matrix regulates fetoplacental angiogenesis in severe fetal growth restriction Clinical science (London, England : 1979) 2021-04-27 [PMID: 33904582] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Abinaya Sundari Thooyamani, Asok Mukhopadhyay PDGFR alpha mediated survival of myofibroblasts inhibit satellite cell proliferation during aberrant regeneration of lacerated skeletal muscle Scientific Reports 2021-01-08 [PMID: 33420132]
• Kim J, Lee C, Shin Y et al. Small extracellular vesicles from tonsil-derived mesenchymal stromal cells alleviate activation of hepatic stellate cells and liver fibrosis through miR-486-5p Molecular therapy : the journal of the American Society of Gene Therapy 2020-12-18 [PMID: 33348053] (WB, Human, Mouse)
• Yang M, Liu Q, Huang T et al. Dysfunction of estrogen-related receptor alpha-dependent hepatic VLDL secretion contributes to sex disparity in NAFLD/NASH development Theranostics 2020-09-08 [PMID: 33042259] (IHC-P, Mouse)
• Wang Q, Sundar Ik, Blum Jl Et Al. Prenatal Exposure to E-Cigarette Aerosols Leads to Sex-dependent Pulmonary Extracellular Matrix Remodeling and Myogenesis in Offspring Mice Am. J. Respir. Cell Mol. Biol. 2020-08-27 [PMID: 32853043]
• Ma Z, Li L, Livingston MJ et al. p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease J. Clin. Invest. 2020-09-01 [PMID: 32804155] (IF/IHC, Mouse)
• Wang Q, Sundar I, Li D et al. E-cigarette-Induced Pulmonary Inflammation and Dysregulated Repair are Mediated by nAChR ?7 Receptor: Role of nAChR ?7 in ACE2 Covid-19 receptor regulation Research Square 2022-11-07 [PMID: 32702718] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Wang Q, Sundar IK, Li D et al. E-cigarette-induced pulmonary inflammation and dysregulated repair are mediated by nAChR alpha 7 receptor: role of nAChR alpha 7 in SARS-CoV-2 Covid-19 ACE2 receptor regulation Respir. Res. 2020-06-18 [PMID: 32552811] (WB, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 format of this antibody.
• Zhang X, Liu S, Zhang C et al. The role of AMPK alpha 2 in the HFD-induced nonalcoholic steatohepatitis Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2020-06-01 [PMID: 32502647] (WB, Mouse)
• Li Y, Yuan J, Chen F et al. Long noncoding RNA SAM promotes myoblast proliferation through stabilizing Sugt1 and facilitating kinetochore assembly Nat Commun 2020-06-01 [PMID: 32483152] (IF/IHC, Mouse)
• Ren Yc, Zhao Q, He Y et al. Legumain promotes fibrogenesis in chronic pancreatitis via activation of transforming growth factor Beta 1 J. Mol. Med. 2020-05-15 [PMID: 32415356] (ICC/IF, Mouse)
• Herum KM, Romaine A, Wang A et al. Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin J Am Heart Assoc 2020-02-04 [PMID: 32000579] (WB, Mouse)
• Wang Q, Khan N A et al Dysregulated repair and inflammatory responses by e-cigarette-derived inhaled nicotine and humectant propylene glycol in a sex-dependent manner in mouse lung FASEB Bioadv 2019-12-12 [PMID: 31825014] (FLOW, FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Nukaga T, Sakai D et al. Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model. JOR Spine 2019-01-06 [PMID: 31463464] (IF/IHC, Rat)
• Waise S, Parker R, Rose-Zerilli MJJ et al. An optimised tissue disaggregation and data processing pipeline for characterising fibroblast phenotypes using single-cell RNA sequencing Sci Rep 2019-07-03 [PMID: 31270426] (WB, Human)
• Zhu J, Kovacs L, Han W et al. ROS-Dependent Calpain Activation Contributes to Airway and Pulmonary Vascular Remodeling in COPD Antioxid. Redox Signal. 2019-05-15 [PMID: 31088299]
• Napit PR, Ali MH, Shakya M et al. Hydrogen sulphide mitigates homocysteine-induced apoptosis and matrix remodelling in mesangial cells through Akt/FOXO1 signalling cascade Cell. Signal. 2019-05-11 [PMID: 31085234] (WB, Mouse)
• K Wang, S Fang, Q Liu, J Gao, X Wang, H Zhu, Z Zhu, F Ji, J Wu, Y Ma, L Hu, X Shen, D Gao, J Zhu, P Liu, H Zhou TGF-?1/p65/MAT2A pathway regulates liver fibrogenesis via intracellular SAM EBioMedicine, 2019-03-27;0(0):. 2019-03-27 [PMID: 30926424] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Kovacs L, Cao Y, Han W et al. PFKFB3 in Smooth Muscle Promotes Vascular Remodeling in Pulmonary Arterial Hypertension Am. J. Respir. Crit. Care Med. 2019-02-28 [PMID: 30817168] (WB, ICC/IF, Mouse, Human)
• Wang X, Pan J, Liu H et al. AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model Life Sci. 2019-02-18 [PMID: 30790610] (IHC-P, Rat)
• Grigorova YN, Wei W, Petrashevskaya N et al. Dietary Sodium Restriction Reduces Arterial Stiffness, Vascular TGF-B-Dependent Fibrosis and Marinobufagenin in Young Normotensive Rats Int J Mol Sci 2018-10-15 [PMID: 30326586] (WB, Rat)
• Ma Z, Wei Q, Zhang M et al. Dicer deficiency in proximal tubules exacerbates renal injury and tubulointerstitial fibrosis and upregulates Smad2/3. Am. J. Physiol. Renal Physiol. 2018-10-03 [PMID: 30280598] (IF/IHC, Mouse)
• Shen M, Hu M, Fedak PW et al. Cell-Specific Functions of ADAM17 Regulate the Progression of Thoracic Aortic Aneurysm Circ. Res. 2018-06-21 [PMID: 29930147] (Human)
• Oztay F, Sancar-Bas S, Gezginci-Oktayoglu S et al. Exendin-4 partly ameliorates - hyperglycemia-mediated tissue damage in lungs of streptozotocin-induced diabetic mice. Peptides 2018-01-01 [PMID: 29225158] (Mouse)
• Song LJ, Xiang F, Ye H et al. Inhibition of angiotensin II and calpain attenuates pleural fibrosis. Pulm Pharmacol Ther. 2017-10-26 [PMID: 29107090] (Mouse)
• Herum KM, Choppe J, Kumar A et al. Mechanical regulation of cardiac fibroblast profibrotic phenotypes Mol Biol Cell. 2017-07-06 [PMID: 28468977] (Rat)
• Cai P, Kovacs L, Dong S et al. BMP4 inhibits PDGF-induced proliferation and collagen synthesis via PKA-mediated inhibition of calpain-2 in pulmonary artery smooth muscle cells. Am. J. Physiol. Lung Cell Mol. Physiol. 2017-05-01 [PMID: 28235949] (WB, Human)
• Kim J, Wang S, Hyun J et al. Hedgehog Signaling is Associated with Liver Response to Fractionated Irradiation in Mice. Cell. Physiol. Biochem. 2016-11-17 [PMID: 27855416] (WB, Mouse)
• Luo J, Liu X, Liu J et al. Activation of TGF-beta1 by AQP3-Mediated H2O2 Transport into Fibroblasts of a Bleomycin-Induced Mouse Model of Scleroderma Journal of Investigative Dermatology 2016-07-22 [PMID: 27456753] (WB, Mouse)
• Jadhav S, Ajay AK, Trivedi P et al. RNA binding protein Musashi homologue 1 regulates kidney fibrosis by translational inhibition of p21 and Numb mRNA. J. Biol. Chem. 2016-04-28 [PMID: 27129280] (WB, Mouse)
  
  Details:
  Rabbit polyclonal anti-Collagen 1 alpha 1 antibody was used at 1:500 dilution for WB analysis of 25ug lysates from contralateral kidney (CoK) and unilateral ureteral obstruction (UUO) kidneys of vehicle and oleic acid-treated mice (FIGURE 5).
• Livingston MJ, Ding HF, Huang S et al. Persistent activation of autophagy in kidney tubular cells promotes renal interstitial fibrosis during unilateral ureteral obstruction. Autophagy. 2016-04-28 [PMID: 27123926]
• Bornes TD, Jomha NM, Mulet-Sierra A, Adesida AB Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold. Tissue Eng Part C Methods 2016-03-01 [PMID: 26651081] (ICC/IF)
• Yang P, Hong MS, Fu C et al. Preexisting smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesions. Surgery 2016-02-01 [PMID: 26387788] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Mouse)
• Chen Y, Huang J, Hu C, Hua K. Relationship of advanced glycation end products and their receptor to pelvic organ prolapse. Int J Clin Exp Pathol 2015-01-01 [PMID: 26045736] (IF/IHC, Human)
• Ovet H, Oztay F. The Copper Chelator Tetrathiomolybdate Regressed Bleomycin-Induced Pulmonary Fibrosis in Mice, by Reducing Lysyl Oxidase Expressions. Biol Trace Elem Res. 2014-10-28 [PMID: 25349139] (WB, Mouse)
• Mancuso L, Gualerzi A, Boschetti F et al. Decellularized ovine arteries as small-diameter vascular grafts. Biomed Mater 2014-08-01 [PMID: 25050540] (IHC-P, Sheep)
  
  Details:
  Collagen I antibody used for IHC-P on decellularized ovine/ovine arteries - heat induced antigen retrieval, primary used at 1:100 dilution in PBS/incubated overnight at 4C detected with goat anti-rabbit immunoglobulin conjugated with alkaline phosphatase and Fast Red TR/Naphtol AS-MX.
• Clapper JR, Hendricks MD, Gu G et al. Diet-induced mouse model of fatty liver disease and nonalcoholic steatohepatitis reflecting clinical disease progression and methods of assessment. Am J Physiol Gastrointest Liver Physiol. 2013-10-01 [PMID: 23886860] (WB, Mouse)
• Liu CF, Breidenbach A, Aschbacher-Smith L et al. A Role for Hedgehog Signaling in the Differentiation of the Insertion Site of the Patellar Tendon in the Mouse. PLoS One 2013-06-10 [PMID: 23762363] (ICC/IF, IHC-Fr, Mouse)
• Trevaskis JL, Griffin PS, Wittmer C et al. Glucagon-like peptide-1 (GLP-1) receptor agonism improves metabolic, biochemical and histopathological indices of nonalcoholic steatohepatitis (NASH) in mice. American Journal of Physiology. Gastrointesti. [PMID: 22268099] (WB, Mouse)
• Liu CF, Aschbacher-Smith L, Barthelery NJ, Dyment N, Butler D, Wylie C. Spatial and temporal expression of molecular markers and cell signals during normal development of the mouse patellar tendon. Tissue Eng Part A;18(5-6):598-608. 2012-03-01 [PMID: 21939397] (IF/IHC, ICC/IF, Mouse)

Reviews for Collagen I alpha 1 Antibody (NBP1-30054) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer WB Mouse 10/02/2020

Summary

• Application: Western Blot
• Sample Tested: Mouse Pancreas
• Species: Mouse
• Lot: lot SH8204

reviewed by: wang xuyang IHC-P Rat 12/20/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Adult heart
• Species: Rat
• Lot: lot SH317a

FAQs for Collagen I alpha 1 Antibody (NBP1-30054). (Showing 1 - 7 of 7 FAQs).

1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
   • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
   • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
3. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
   • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
4. How long does delivery take for your collagen 1 alpha 1 antibody?
   • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
5. Do you have any references on IHC-P performed on sheep tissue?
   • Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.
6. I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype
   • NBP1-30054 is a rabbit IgG polyclonal antibody.
7. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
   • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

Bioinformatics

• Gene Symbol: COL1A1
• Entrez:
  • 1277(Human)
  • 12842(Mouse)
  • 29393(Rat)
• Uniprot:
  • P02452()