Collagen I alpha 1 Antibody

Images

 
Western Blot: Collagen I alpha 1 Antibody [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the ~ 140k collagen protein.
Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils ...read more
Simple Western: Collagen I alpha 1 Antibody [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, Simple Western, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Collagen I alpha 1 Antibody Summary

Immunogen
Synthetic peptide from the C-terminal region of human collagen I alpha1 telopeptide sequence.
Specificity
Specific for the ~ 140 kDa telopeptide portion of the collagen I alpha 1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL1A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
Application Notes
Use in Immunohistochemistry-Frozen sections was reported in the scientific literature (PMID: 21939397), IHC reactivity reported in scientific literature (PMID: 26045736).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-30054 in the following applications:

Reactivity Notes

Based on the homology of the immunogen this antibody is expected to recognize the collagen I alpha1 polypeptide in all mammals, birds, and amphibians.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen I alpha 1 Antibody

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • OI4
  • pro-alpha-1 collagen type 1

Background

Collagen type I is present in soft connective tissue and bone where it represents approximately 90% of the organix matrix. Collagen type I is produced from procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. The propeptide levels can be measured in serum as indicators of collagen production. Elevated values of these propeptides in serum are found in patients with various bone metabolism defects. Procollagen type I C-terminal propeptide (PICP) has a molecular weight of approximately 100 kDa and the normal range in serum is 100-300 ug/ml.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen I alpha 1 Antibody (NBP1-30054)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Sheep.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(7)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-30054 Applications Species
Kim J, Wang S, Hyun J et al. Hedgehog Signaling is Associated with Liver Response to Fractionated Irradiation in Mice. Cell. Physiol. Biochem. Nov 17 2016 [PMID: 27855416] (WB, Mouse) WB Mouse
Jadhav S, Ajay AK, Trivedi P et al. RNA binding protein Musashi homologue 1 regulates kidney fibrosis by translational inhibition of p21 and Numb mRNA. J. Biol. Chem. Apr 28 2016 [PMID: 27129280] (WB, Mouse)

Details:
Rabbit polyclonal anti-Collagen 1 alpha 1 antibody was used at 1:500 dilution for WB analysis of 25ug lysates from contralateral kidney (CoK) and unilateral ureteral obstruction (UUO) kidneys of vehicle and oleic acid-treated mice (FIGURE 5).
WB Mouse
Livingston MJ, Ding HF, Huang S et al. Persistent activation of autophagy in kidney tubular cells promotes renal interstitial fibrosis during unilateral ureteral obstruction. Autophagy. Apr 28 2016 [PMID: 27123926]
Bornes TD, Jomha NM, Mulet-Sierra A, Adesida AB Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold. Tissue Eng Part C Methods 2016 Mar [PMID: 26651081] (ICC/IF) ICC/IF
Chen Y, Huang J, Hu C, Hua K. Relationship of advanced glycation end products and their receptor to pelvic organ prolapse. Int J Clin Exp Pathol 2015 [PMID: 26045736] (IHC, Human) IHC Human
Ovet H, Oztay F. The Copper Chelator Tetrathiomolybdate Regressed Bleomycin-Induced Pulmonary Fibrosis in Mice, by Reducing Lysyl Oxidase Expressions. Biol Trace Elem Res. 2014 Oct 28 [PMID: 25349139] (WB, Mouse) WB Mouse
Mancuso L, Gualerzi A, Boschetti F et al. Decellularized ovine arteries as small-diameter vascular grafts. Biomed Mater 2014 Aug 01 [PMID: 25050540] (IHC-P, Sheep)

Details:
Collagen I antibody used for IHC-P on decellularized ovine/ovine arteries - heat induced antigen retrieval, primary used at 1:100 dilution in PBS/incubated overnight at 4C detected with goat anti-rabbit immunoglobulin conjugated with alkaline phosphatase and Fast Red TR/Naphtol AS-MX.
IHC-P Sheep
Clapper JR, Hendricks MD, Gu G et al. Diet-induced mouse model of fatty liver disease and nonalcoholic steatohepatitis reflecting clinical disease progression and methods of assessment. Am J Physiol Gastrointest Liver Physiol. 2013 Oct 1 [PMID: 23886860] (WB, Mouse) WB Mouse
Liu CF, Breidenbach A, Aschbacher-Smith L et al. A Role for Hedgehog Signaling in the Differentiation of the Insertion Site of the Patellar Tendon in the Mouse. PLoS One 2013 Jun 10 [PMID: 23762363] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Trevaskis JL, Griffin PS, Wittmer C et al. Glucagon-like peptide-1 (GLP-1) receptor agonism improves metabolic, biochemical and histopathological indices of nonalcoholic steatohepatitis (NASH) in mice. American Journal of Physiology. Gastrointesti. [PMID: 22268099] (WB, Mouse) WB Mouse
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Collagen I alpha 1 Antibody (NBP1-30054). (Showing 1 - 10 of 10 FAQs).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. Can this collagen 1 alpha 1 antibody be conjugated?
    • Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
  4. What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
    • This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
  5. What is the immunogen for this collagen antibody?
    • Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
  6. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  7. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  8. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
    • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.
  9. Do you have any references on IHC-P performed on sheep tissue?
    • Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.
  10. I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype
    • NBP1-30054 is a rabbit IgG polyclonal antibody.

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Bioinformatics

Gene Symbol COL1A1
Entrez
Uniprot