Western Blot: Collagen I alpha 1 Antibody [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the ~ 140k collagen protein.
Simple Western: Collagen I alpha 1 Antibody [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was ...read more
Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils ...read more
Synthetic peptide corresponding to amino acid residues specific to the collagen 1, alpha 1 telopeptide conjugated to KLH
Specific for the ~ 140 kDa telopeptide portion of the collagen I alpha 1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in Immunohistochemistry-Frozen sections was reported in the scientific literature (PMID: 21939397), IHC reactivity reported in scientific literature (PMID: 26045736).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
140 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-30054 in the following applications:
Based on the homology of the immunogen this antibody is expected to recognize the collagen I alpha1 polypeptide in all mammals, birds, and amphibians.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Immunogen affinity purified
Alternate Names for Collagen I alpha 1 Antibody
Alpha-1 type I collagen
Collagen 1 alpha 1
collagen alpha 1 chain type I
collagen alpha-1(I) chain
Collagen I alpha 1
collagen of skin, tendon and bone, alpha-1 chain
collagen, type I, alpha 1
pro-alpha-1 collagen type 1
Collagen type I is present in soft connective tissue and bone where it represents approximately 90% of the organix matrix. Collagen type I is produced from procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. The propeptide levels can be measured in serum as indicators of collagen production. Elevated values of these propeptides in serum are found in patients with various bone metabolism defects. Procollagen type I C-terminal propeptide (PICP) has a molecular weight of approximately 100 kDa and the normal range in serum is 100-300 ug/ml.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Collagen I alpha 1 Antibody (NBP1-30054). (Showing 1 - 10 of 10 FAQs).
We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
Can this collagen 1 alpha 1 antibody be conjugated?
Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
What is the immunogen for this collagen antibody?
Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
How long does delivery take for your collagen 1 alpha 1 antibody?
Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
What secondary antibody do you recommend for collagen I alpha 1 antibodies?
Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.
Do you have any references on IHC-P performed on sheep tissue?
Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.
I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.