Immunogen | Collagen III alpha 1/COL3A1 from human and bovine placenta (Uniprot: P02461) |
Localization | Extracellular matrix |
Specificity | Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen III alpha 1/COL3A1 and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | COL3A1 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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Application Notes | This product has been tested by dot Blot, western blot, and IHC and is useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis. Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50. Apparent MW in kDa on Simple Western was 139 kDa; matrix was 12-230 kDa. |
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Storage | Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles. |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Preservative | 0.01% Sodium Azide |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 04/07/2021 |
Summary
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reviewed by:
Wangsheng Wang |
WB | Human | 06/07/2018 |
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reviewed by:
David Sun |
IHC-Fr | Feline | 02/18/2018 |
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reviewed by:
Verified Customer |
IHC-P | Rat | 12/20/2017 |
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reviewed by:
Sandra Lourenssen |
IHC-P | Rat | 07/20/2016 |
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reviewed by:
Verified Customer |
IF | Human | 04/02/2015 |
Summary
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reviewed by:
Christian Zuppinger |
IF | Human | 10/04/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for Collagen III alpha 1/COL3A1 Antibody (NB600-594)Discover more about diseases related to Collagen III alpha 1/COL3A1 Antibody (NB600-594).
| Pathways for Collagen III alpha 1/COL3A1 Antibody (NB600-594)View related products by pathway.
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Verified Customer 04/07/2021 |
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Application: | WB | |
Species: | Mouse |
Wangsheng Wang 06/07/2018 |
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Application: | WB | |
Species: | Human |
David Sun 02/18/2018 |
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Application: | IHC-Fr | |
Species: | Feline |