Collagen III alpha 1/COL3A1 Antibody

Images

 
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4C Secondary antibody: DyLight 649 Goat ...read more
Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human primary ventricular cardiac fibroblasts were stained with anti-Collagen III antibody. Cells were cultured for 3 days in ...read more
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Analysis of Biotin conjugate of NB600-594. Strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and ...read more
Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III alpha 1 expression in HT-1080 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic ...read more
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue.
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed ...read more
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Analysis of FITC conjugate of NB600-594. Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Collagen III alpha 1/COL3A1 Antibody Summary

Immunogen
Collagen Type III from human and bovine placenta.
Localization
Extracellular matrix
Specificity
Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL3A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • ELISA 1:5000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:100
Application Notes
Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for in immunoblotting.
ICC/IF data from customer review.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB600-594 in the following applications:

Publications
Read Publications using
NB600-594 in the following applications:

  • IHC
    2 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21479341). Rat reactivity reported in scientific literature (PMID: 23370982).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.125M Sodium Borate, 75mM NaCl and 5mM EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen III alpha 1/COL3A1 Antibody

  • alpha1 (III) collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • EDS4A
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534

Background

These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for Collagen III alpha 1/COL3A1 Antibody (NB600-594)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(2)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB600-594 Applications Species
Schreckenberg R, Horn AM, da Costa Rebelo RM et al. Effects of 6-months' Exercise on Cardiac Function, Structure and Metabolism in Female Hypertensive Rats-The Decisive Role of Lysyl Oxidase and Collagen III Front Physiol 2017 Aug 21 [PMID: 28824452] (WB, Rat)

Details:
Collagen III antibody was used to look at the effect of running training on the expression of COL III in rats that were either sedentary or subjected to running training
WB Rat
Teo Z, Chan JSK, Chong HC et al. Angiopoietin-like 4 induces a B-catenin-mediated upregulation of ID3 in fibroblasts to reduce scar collagen expression Sci Rep 2017 Jul 24 [PMID: 28740178] (WB, Human) WB Human
Lee HJ, Kim YS, Ok JH et al. Effect of a single subacromial prednisolone injection in acute rotator cuff tears in a rat model. Knee Surg Sports Traumatol Arthrosc 2013 Jan 31 [PMID: 23370982] (IHC, Rat) IHC Rat
Mak KM, Chu E, Lau KH, Kwong AJ. Liver fibrosis in elderly cadavers: localization of collagen types I, III, and IV, alpha-smooth muscle actin, and elastic fibers. The Anatomical Record, 2012; vol 295, pages 1159-1167. 2012 Jul [PMID: 22644959]
Mak KM, Kwong AJ, Chu E et al. Hepatic steatosis, fibrosis, and cancer in elderly cadavers. The Anatomical Record, 2012; vol 295, pages 40-50. 2012 Jan [PMID: 22139908]
Hechler B, Gachet C Comparison of two murine models of thrombosis induced by atherosclerotic plaque injury. Thromb Haemost 2011 May [PMID: 21479341] (IHC, Mouse) IHC Mouse
Dahl SL, Kypson AP, Lawson JH et al. Readily available tissue-engineered vascular grafts. Sci Transl Med 2011 Feb 2 [PMID: 21289273]
Sales VL, Engelmayr GC Jr, Johnson JA Jr et al. Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells Circulation [PMID: 17846326]

Details:
Used the Biotin form of this antibody.
Sales VL, Engelmayr GC Jr, Johnson JA Jr et al. Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells Circulation

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NB120-6580).

Reviews for Collagen III alpha 1/COL3A1 Antibody (NB600-594) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rat.
We have 3 reviews tested in 2 application: IHC-P, IF.

Reviews using NB600-594:
Filter by Applications
IHC-P
(1)
IF
(2)
All Applications
Filter by Species
Human
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Collagen III alpha 1/COL3A1 NB600-594
Enlarge
reviewed by:
Anonymous
IHC-P Rat 07/20/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedrat colon
SpeciesRat
Lot33577
Immunofluorescence Collagen III alpha 1/COL3A1 NB600-594
Enlarge
reviewed by:
Jessica Prieto
IF Human 04/02/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedHT-1080 (Fibrosarcoma)
SpeciesHuman

Blocking

Blocking Details5% BSA (prepared in 1X PBS) for 20 minutes at room temperature

Primary Anitbody

Dilution Ratio1:1000, 1 hour, room temperature

Secondary Antibody

Secondary DescriptionGoat Anti-Rabbit IgG, Alexa Fluor 594
Secondary Manufacturer Cat#A11012
Secondary Concentration1:500 dilution

Details

Detection NotesHT-1080 (positive control), Human Astrocytes (negative control)
Fixation Details4% PFA fix for 20 minutes at room temperature
Wash Description1X PBS, 5 minutes, 3 washes
Immunofluorescence Collagen III alpha 1/COL3A1 NB600-594
Enlarge
reviewed by:
Anonymous
IF Human 10/04/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedhuman primary ventricular cardiac fibroblasts (from ScienCell)
SpeciesHuman
Lot30240

Blocking

Blocking Details1 mg/ml BSA in PBS, 30 min, room temperature

Primary Anitbody

Dilution Ratio1:100 in BSA/PBS overnight at 4C

Secondary Antibody

Secondary DescriptionGoat anti rabbit Alexa488
Secondary Manufacturer Cat#A-21222
Secondary Concentration1:200

Details

Detection Notesconfocal microscopy, Zeiss LSM710
Fixation Detailsfixation with 3% PFA in PBS for 15 min at room temperature
Wash Descriptionwashes with PBS, usually 3 times for 5 minutes

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen III alpha 1/COL3A1 Antibody (NB600-594). (Showing 1 - 3 of 3 FAQs).

  1. May I know if you carry the un-conjugated version of NB120-6580 (biotinylated anti-Collagen Type iII)?
    • I would recommend this catalog number for an unconjugated anti-Collagen Type III antibody: NB600-594.
  2. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-594, the dilution range is 1:50-1:200.
  3. I am wondering if you could please confirm for me whether this product Collagen III antibody - catalogue # NB600-594 is able to be used under denaturing conditions with Western blots? Or whether it only binds to the collagen in its natural (non-denatured)
    • The Western blot image is not generated from native collagen - reducing conditions were used to obtain the image. The intact native molecule is too large to be run on standard 4-20% gels so the sample was run under reducing conditions. The image shows the sub-unit strand(s), not the intact collagen that consists of 3 subunit strands. These are not considered optimal or specific binding conditions for the anti-collagen antibodies, however the decision was made to run and post this on the website as an example.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
07/20/2016
Application: IHC-P
Species: Rat

Jessica Prieto
04/02/2015
Application: IF
Species: Human

Anonymous
10/04/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol COL3A1
Entrez