Collagen III alpha 1/COL3A1 Antibody

Images

 
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: ...read more
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4C Secondary antibody: DyLight 649 Goat ...read more
Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human primary ventricular cardiac fibroblasts were stained with anti-Collagen III antibody. Cells were cultured for 3 days in ...read more
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed ...read more
Biological Strategies: Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Immunodetection of ID3, COL1A2 and COL3A1 in human dermal fibroblasts subjected to treatment with or without cANGPTL4 (12 ...read more
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for ...read more
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Western blot for COL3A1 protein with PVDF protein staining to control for protein loading. Image collected and cropped by CiteAb from the following ...read more
Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III alpha 1 expression in HT-1080 cells. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue.
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III (k, positive staining indicated by arrows) immunohistochemistry staining in 13 weeks old male offspring. Left panel = Sham, Middle ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, ShSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

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Catalog# & Conjugate Size Price

Collagen III alpha 1/COL3A1 Antibody Summary

Immunogen
Collagen III alpha 1/COL3A1 from human and bovine placenta (Uniprot: P02461)
Localization
Extracellular matrix
Specificity
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL3A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 1:100
  • Simple Western
  • Western Blot 1:1000-1:10000
Application Notes
Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50. Apparent MW in kDa on Simple Western was 139 kDa; matrix was 12-230 kDa.
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 7 Reviews rated 4.7
using
NB600-594 in the following applications:

Publications
Read Publications using
NB600-594 in the following applications:

  • 4 publications
  • IHC
    7 publications
  • 6 publications
  • WB
    7 publications

Reactivity Notes

Use in Sheep reported in scientific literature (PMID:34451177). This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens.
Mouse reactivity reported in multiple pieces of scientific literature. Rat reactivity reported in scientific literature (PMID: 23370982). Feline reactivity reported in scientific literature (PMID: 33091431).

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Collagen III alpha 1/COL3A1 Antibody

  • alpha1 (III) collagen
  • alpha-1 type III collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • collagenIII
  • Collagen-III
  • EDS4A
  • EDSVASC
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534
  • PMGEDSV

Background

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen III alpha 1/COL3A1 Antibody (NB600-594)(28)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Feline, Sheep.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(7)
IHC-P
(6)
WB
(7)
All Applications
Filter By Species
Human
(5)
Mouse
(7)
Rat
(4)
Feline
(2)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB600-594 Applications Species
Willemse J, Verstegen M, Vermeulen A et al. Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion Materials Science and Engineering: C Sep 12 2019 [PMID: 31923991] (IHC-P, IHC, Human) IHC-P, IHC Human
Dahl SL, Kypson AP, Lawson JH et al. Readily available tissue-engineered vascular grafts. Sci Transl Med 2011 Feb 2 [PMID: 21289273]
Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis Jul 13 1905
Guillaume E, Zacharopoulou M, Reynolds B et al. Additional Value of Second Harmonic Generation Microscopy in the Diagnosis of Feline Collagen Type III Glomerulopathy Journal of Comparative Pathology Oct 1 2021 [PMID: 34686276] (IHC-P, Feline) IHC-P Feline
Maity P, Singh K, Krug L Et al. Persistent JunB activation in fibroblasts disrupts stem cell niche interactions enforcing skin aging Cell reports Aug 31 2021 [PMID: 34469740] (ICC/IF, Mouse) ICC/IF Mouse
Oka SI, Byun J, Huang CY, Imai N, Ralda G, Zhai P, Xu X, Kashyap S, Warren JS, Alan Maschek J, Tippetts TS, Tong M, Venkatesh S, Ikeda Y, Mizushima W, Kashihara T, Sadoshima J. Nampt Potentiates Antioxidant Defense in Diabetic Cardiomyopathy Apr 30 2021 [PMID: 33928788] (WB, Mouse) WB Mouse
Li W, Qi N, Wang S et al. miR-455-5p regulates atrial fibrillation by targeting suppressor of cytokines signaling 3 Journal of physiology and biochemistry Apr 1 2021 [PMID: 33792885]
Huang L, Zhang Y, Yang J et al. EGR 1 plays a protective role in AMPK inhibitor compound C induced apoptosis through ROS induced ERK activation in skin cancer cellsAnti fibrotic effects and the mechanism of action of miR 29c in silicosis Molecular medicine reports Apr 1 2021 [PMID: 33649785]
Li H, Wang Y, Liu J, et al. Endothelial Klf2-Foxp1-TGF beta signal mediates the inhibitory effects of simvastatin on maladaptive cardiac remodeling Theranostics Jan 1 2021 [PMID: 33408770] (WB) WB
Paul K, Darzi S, Del Borgo M et al. Vaginal delivery of tissue engineered endometrial mesenchymal stem/stromal cells in an aloe vera-alginate hydrogel alleviates maternal simulated birth injury Applied Materials Today Mar 1 2021 (ICC/IF, Human) ICC/IF Human
Show All 28 Publications.

Reviews for Collagen III alpha 1/COL3A1 Antibody (NB600-594) (7) 4.77

Average Rating: 4.7
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Rat, Feline.

Reviews using NB600-594:
Filter by Applications
WB
(2)
IHC-Fr
(1)
IHC-P
(2)
IF
(2)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
Rat
(2)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Collagen III alpha 1/COL3A1 NB600-594
Enlarge
4
reviewed by:
Shinichi Oka
WB Mouse 04/07/2021
View

Summary

ApplicationWestern Blot
Sample TestedHeart lysates
SpeciesMouse
Lot42255

Comments

CommentsMouse heart lysate was prepared after 3 months of normal diet (ND) and high fat diet (HFD) consumption. Western blot was performed with anti-Col3a1 antibody.
Western Blot Collagen III alpha 1/COL3A1 NB600-594
Enlarge
5
reviewed by:
Verified Customer
WB Human 06/07/2018
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell lysate
SpeciesHuman
Lot37149
Immunohistochemistry-Frozen Collagen III alpha 1/COL3A1 NB600-594
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5
reviewed by:
Verified Customer
IHC-Fr Feline 02/18/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedConnective tissue
SpeciesFeline
Lot39661
Immunohistochemistry-Paraffin Collagen III alpha 1/COL3A1 NB600-594
Enlarge
5
reviewed by:
wang xuyang
IHC-P Rat 12/20/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedH9c2 whole cell lysate
SpeciesRat
Lotlot37797
Immunohistochemistry-Paraffin Collagen III alpha 1/COL3A1 NB600-594
Enlarge
4
reviewed by:
Verified Customer
IHC-P Rat 07/20/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedrat colon
SpeciesRat
Lot33577
Immunofluorescence Collagen III alpha 1/COL3A1 NB600-594
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5
reviewed by:
Jessica Ochoa
IF Human 04/02/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedHT-1080 (Fibrosarcoma)
SpeciesHuman

Blocking

Blocking Details5% BSA (prepared in 1X PBS) for 20 minutes at room temperature

Primary Anitbody

Dilution Ratio1:1000, 1 hour, room temperature

Secondary Antibody

Secondary DescriptionGoat Anti-Rabbit IgG, Alexa Fluor 594
Secondary Manufacturer Cat#A11012
Secondary Concentration1:500 dilution

Details

Detection NotesHT-1080 (positive control), Human Astrocytes (negative control)
Fixation Details4% PFA fix for 20 minutes at room temperature
Wash Description1X PBS, 5 minutes, 3 washes
Immunofluorescence Collagen III alpha 1/COL3A1 NB600-594
Enlarge
5
reviewed by:
Verified Customer
IF Human 10/04/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedhuman primary ventricular cardiac fibroblasts (from ScienCell)
SpeciesHuman
Lot30240

Blocking

Blocking Details1 mg/ml BSA in PBS, 30 min, room temperature

Primary Anitbody

Dilution Ratio1:100 in BSA/PBS overnight at 4C

Secondary Antibody

Secondary DescriptionGoat anti rabbit Alexa488
Secondary Manufacturer Cat#A-21222
Secondary Concentration1:200

Details

Detection Notesconfocal microscopy, Zeiss LSM710
Fixation Detailsfixation with 3% PFA in PBS for 15 min at room temperature
Wash Descriptionwashes with PBS, usually 3 times for 5 minutes

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen III alpha 1/COL3A1 Antibody (NB600-594). (Showing 1 - 3 of 3 FAQs).

  1. May I know if you carry the un-conjugated version of NB120-6580 (biotinylated anti-Collagen Type iII)?
    • I would recommend this catalog number for an unconjugated anti-Collagen Type III antibody: NB600-594.
  2. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-594, the dilution range is 1:50-1:200.
  3. I am wondering if you could please confirm for me whether this product Collagen III antibody - catalogue # NB600-594 is able to be used under denaturing conditions with Western blots? Or whether it only binds to the collagen in its natural (non-denatured)
    • The Western blot image is not generated from native collagen - reducing conditions were used to obtain the image. The intact native molecule is too large to be run on standard 4-20% gels so the sample was run under reducing conditions. The image shows the sub-unit strand(s), not the intact collagen that consists of 3 subunit strands. These are not considered optimal or specific binding conditions for the anti-collagen antibodies, however the decision was made to run and post this on the website as an example.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NB120-6580
FITC NBP2-46878

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Recent Reviews

4.7
5
5
4
2
3
0
2
0
1
0

Shinichi Oka
04/07/2021
Application: WB
Species: Mouse

Verified Customer
06/07/2018
Application: WB
Species: Human

Verified Customer
02/18/2018
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol COL3A1