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Collagen III alpha 1/COL3A1 Antibody

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Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: ...read more
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4C Secondary antibody: DyLight 649 Goat ...read more
Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human primary ventricular cardiac fibroblasts were stained with anti-Collagen III antibody. Cells were cultured for 3 days in ...read more
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed ...read more
Biological Strategies: Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Immunodetection of ID3, COL1A2 and COL3A1 in human dermal fibroblasts subjected to treatment with or without cANGPTL4 (12 ...read more
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Livers were harvested at 9 wpi, formalin-fixed, and then cryosectioned and stained for markers of inflammation and liver fibrosis. Maximum ...read more
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Western blot for COL3A1 protein with PVDF protein staining to control for protein loading. Image collected and cropped by CiteAb from the following ...read more
Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III alpha 1 expression in HT-1080 cells. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue.
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III (k, positive staining indicated by arrows) immunohistochemistry staining in 13 weeks old male offspring. Left panel = Sham, Middle ...read more
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for ...read more
anti collagen III antibody ( Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed ...read more
Immunohistochemistry of Rabbit Anti-collagen type III antibody. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat ...read more
Immunohistochemistry of Rabbit Collagen III alpha 1/COL3A1 Antibody. Tissue: FFPE normal human spleen tissues (10X). Antigen Retrieval: 0.01 M sodium citrate buffer for 20 minutes. Primary Antibody: Anti-Collagen Type ...read more
Markers of liver inflammation TNF-alpha, IL-1beta (a,b), macrophage markers MCP-1, CD68 and F4/80 (c-e), stellate cell activation marker alpha- SMA (f) and fibrous deposition of collagen 1a1, collagen III, and total ...read more
cANGPTL4 regulates COL1A2 and COL3A1 expression in fibroblasts via a bHLH-dependent signaling mechanism. (g-i) Immunodetection of ID3, COL1A2 and COL3A1 in human dermal fibroblasts subjected to the indicated treatments. ...read more
Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C and E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, and Nanog levels in Tlk1-KD ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, ShSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

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Catalog# & Conjugate Size Price

Collagen III alpha 1/COL3A1 Antibody Summary

Description
This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Immunogen
Collagen III alpha 1/COL3A1 from human and bovine placenta (Uniprot: P02461)
Localization
Extracellular matrix
Specificity
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen III alpha 1/COL3A1 and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL3A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:50000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 1:100
  • Simple Western
  • Western Blot 1:1000-1:10000
Application Notes
This product has been tested by dot Blot, western blot, and IHC and is useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50. Apparent MW in kDa on Simple Western was 139 kDa; matrix was 12-230 kDa.
Reviewed Applications
Read 7 Reviews rated 4.7
using
NB600-594 in the following applications:

Publications
Read Publications using
NB600-594 in the following applications:

Reactivity Notes

This antibody reacts with most mammalian Collagen III alpha 1/COL3A1 and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens.

Mouse reactivity reported in multiple pieces of scientific literature.
Rat reactivity reported in scientific literature (PMID: 23370982)
Feline reactivity reported in scientific literature (PMID: 33091431)

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Collagen III alpha 1/COL3A1 Antibody

  • alpha1 (III) collagen
  • alpha-1 type III collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • collagenIII
  • Collagen-III
  • EDS4A
  • EDSVASC
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534
  • PMGEDSV

Background

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen III alpha 1/COL3A1 Antibody (NB600-594)(44)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Feline, Mammal, Sheep.

We have publications tested in 8 applications: B/N, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
B/N
(1)
FLOW
(1)
ICC/IF
(8)
IF/IHC
(5)
IHC
(1)
IHC-Fr
(1)
IHC-P
(7)
WB
(10)
All Applications
Filter By Species
Human
(7)
Mouse
(11)
Rat
(5)
Feline
(2)
Mammal
(1)
Sheep
(2)
All Species
Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using NB600-594 Applications Species
Lam Y, Chan C, Geng L et al. APLNR marks a cardiac progenitor derived with human induced pluripotent stem cells bioRxiv 2023-02-23 (ICC/IF, Human) ICC/IF Human
Ito A, Ohnuki Y, Suita K et al. Effects of the angiotensin-converting enzyme inhibitor captopril on occlusal-disharmony-induced cardiac dysfunction in mice Scientific reports 2023-11-15 [PMID: 37968296] (WB, Mouse)

Details:
1:1000 dilution
WB Mouse
Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis 1905-07-13
Ha H, Lee CH, Lee KS et al. Shape-Configurable Mesh for Hernia Repair by Synchronizing Anisotropic Body Motion Small (Weinheim an der Bergstrasse, Germany) 2023-07-25 [PMID: 37490554] (IHC, Rat) IHC Rat
Zhao T, Chu Z, Chu C et al. Macrophages induce gingival destruction via Piezo1-mediated MMPs-degrading collagens in periodontitis Frontiers in Immunology 2023-05-16 [PMID: 37261355] (ICC/IF, Human, Mouse)

Details:
Dilution: 1:100
ICC/IF Human, Mouse
Antonova LV, Sevostianova VV, Silnikov VN et al. Comparison of the Patency and Regenerative Potential of Biodegradable Vascular Prostheses of Different Polymer Compositions in an Ovine Model International journal of molecular sciences 2023-05-10 [PMID: 37239889] (IHC-Fr, Sheep) IHC-Fr Sheep
Su CY, Liu TY, Wang HV, Yang WC Histopathological Study on Collagen in Full-Thickness Wound Healing in Fraser's Dolphins (Lagenodelphis hosei) Animals : an open access journal from MDPI 2023-05-18 [PMID: 37238111] (IHC-P, Mammal)

Details:
1:200 IHC-P dilution. Dolpin is the species
IHC-P Mammal
Wu X, Zhang D, Qiao X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis The European respiratory journal 2023-06-01 [PMID: 36758986] (WB, Mouse) WB Mouse
Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis Jul 13 1905
Lam YY, Chan CH, Geng L et al. APLNR marks a cardiac progenitor derived with human induced pluripotent stem cells Heliyon 2023-07-13 [PMID: 37539315] (ICC/IF) ICC/IF
Show All 44 Publications.

Reviews for Collagen III alpha 1/COL3A1 Antibody (NB600-594) (7) 4.77

Average Rating: 4.7
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Rat, Feline.

Reviews using NB600-594:
Filter by Applications
WB
(2)
IHC-Fr
(1)
IHC-P
(2)
IF
(2)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
Rat
(2)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Collagen III alpha 1/COL3A1 NB600-594
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4
reviewed by:
Shinichi Oka
WB Mouse 04/07/2021
View

Summary

ApplicationWestern Blot
Sample TestedHeart lysates
SpeciesMouse
Lot42255

Comments

CommentsMouse heart lysate was prepared after 3 months of normal diet (ND) and high fat diet (HFD) consumption. Western blot was performed with anti-Col3a1 antibody.
Western Blot Collagen III alpha 1/COL3A1 NB600-594
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5
reviewed by:
Verified Customer
WB Human 06/07/2018
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell lysate
SpeciesHuman
Lot37149
Immunohistochemistry-Frozen Collagen III alpha 1/COL3A1 NB600-594
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5
reviewed by:
Verified Customer
IHC-Fr Feline 02/18/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedConnective tissue
SpeciesFeline
Lot39661
Immunohistochemistry-Paraffin Collagen III alpha 1/COL3A1 NB600-594
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5
reviewed by:
wang xuyang
IHC-P Rat 12/20/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedH9c2 whole cell lysate
SpeciesRat
Lotlot37797
Immunohistochemistry-Paraffin Collagen III alpha 1/COL3A1 NB600-594
Enlarge
4
reviewed by:
Verified Customer
IHC-P Rat 07/20/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedrat colon
SpeciesRat
Lot33577
Immunofluorescence Collagen III alpha 1/COL3A1 NB600-594
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5
reviewed by:
Jessica Ochoa
IF Human 04/02/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedHT-1080 (Fibrosarcoma)
SpeciesHuman
Immunofluorescence Collagen III alpha 1/COL3A1 NB600-594
Enlarge
5
reviewed by:
Verified Customer
IF Human 10/04/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedhuman primary ventricular cardiac fibroblasts (from ScienCell)
SpeciesHuman
Lot30240

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen III alpha 1/COL3A1 Antibody (NB600-594). (Showing 1 - 3 of 3 FAQs).

  1. May I know if you carry the un-conjugated version of NB120-6580 (biotinylated anti-Collagen Type iII)?
    • I would recommend this catalog number for an unconjugated anti-Collagen Type III antibody: NB600-594.
  2. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-594, the dilution range is 1:50-1:200.
  3. I am wondering if you could please confirm for me whether this product Collagen III antibody - catalogue # NB600-594 is able to be used under denaturing conditions with Western blots? Or whether it only binds to the collagen in its natural (non-denatured)
    • The Western blot image is not generated from native collagen - reducing conditions were used to obtain the image. The intact native molecule is too large to be run on standard 4-20% gels so the sample was run under reducing conditions. The image shows the sub-unit strand(s), not the intact collagen that consists of 3 subunit strands. These are not considered optimal or specific binding conditions for the anti-collagen antibodies, however the decision was made to run and post this on the website as an example.

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Recent Reviews

4.7
5
5
4
2
3
0
2
0
1
0

Shinichi Oka
04/07/2021
Application: WB
Species: Mouse

Verified Customer
06/07/2018
Application: WB
Species: Human

Verified Customer
02/18/2018
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol COL3A1
Uniprot