Collagen III alpha 1/COL3A1 Antibody Summary
Collagen Type III from human and bovine placenta. Uniprot ID P02461
Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:1000-1:10000
- ELISA 1:5000-1:50000
- Immunocytochemistry/Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:50-1:200
- Immunohistochemistry-Paraffin 1:50-1:200
- Immunoprecipitation 1:100
Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for in immunoblotting.
ICC/IF data from customer review.
|Read Publications using
NB600-594 in the following applications:
Mouse reactivity reported in multiple pieces of scientific literature. Rat reactivity reported in scientific literature (PMID: 23370982).
Packaging, Storage & Formulations
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Immunogen affinity purified
Alternate Names for Collagen III alpha 1/COL3A1 Antibody
- alpha1 (III) collagen
- alpha-1 type III collagen
- Collagen 3
- collagen alpha-1(III) chain
- Collagen III alpha 1
- collagen, fetal
- collagen, type III, alpha 1
- Ehlers-Danlos syndrome type IV, autosomal dominant
These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for Collagen III alpha 1/COL3A1 Antibody (NB600-594). (Showing 1 - 3 of 3 FAQs).
May I know if you carry the un-conjugated version of NB120-6580 (biotinylated anti-Collagen Type iII)?
- I would recommend this catalog number for an unconjugated anti-Collagen Type III antibody: NB600-594.
What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
- I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-594, the dilution range is 1:50-1:200.
I am wondering if you could please confirm for me whether this product Collagen III antibody - catalogue # NB600-594 is able to be used under denaturing conditions with Western blots? Or whether it only binds to the collagen in its natural (non-denatured)
- The Western blot image is not generated from native collagen - reducing conditions were used to obtain the image. The intact native molecule is too large to be run on standard 4-20% gels so the sample was run under reducing conditions. The image shows the sub-unit strand(s), not the intact collagen that consists of 3 subunit strands. These are not considered optimal or specific binding conditions for the anti-collagen antibodies, however the decision was made to run and post this on the website as an example.
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