Anti-Collagen I alpha 1 Antibodies: Novus Biologicals

NBP1-30054

Collagen I alpha 1 Antibody - Azide and BSA Free

(2 Reviews)

(82 Publications)

0.1 ml

NBP1-30054

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.

Simple Western: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - TNC deficiency reduced kidney fibrosis in animal models.Deletion of TNC (TNC−/−) significantly attenuated the induction of collagen I assessed by IF following UUO by approximate 30% at day 7 & 10 compared with their wild-type littermates (A, n = 4 for each time point, two-way ANOVA p < 0.05). Consistently, the expression of fibrosis markers, such as collagen I alpha , fibronectin & plasminogen activator inhibitor-1 (PAI-1), were significantly lower in TNC−/− mice at UUO day 7 & 10 (B, n = 4 for each time point, two-way ANOVA, p < 0.05). Western blot showed that the proteins of collagen I alpha & alpha -SMA were also significantly reduced in TNC−/− mice at UUO day 7 (C, n = 7, p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36522320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Pathological staining analysis of 28-day animal experiment induced by BAPN. (A), representative images from elastic van Gieson (EVG) staining, along with statistical analysis of elastin degradation. (B), representative images from hematoxylin and eosin (HE) staining are presented, accompanied by statistical analysis of aortic wall thickness. The scale bar for both panels (A) and (B) is 200 μm. (C), immunofluorescence staining of Col1a1, accompanied by quantitative analysis of the Col1a1 positive area in the vascular wall, scale bar 50 μm Image collected and cropped by CiteAb from the following open publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-024-00837-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Am, Av, Ma, Sh, Bv, Ca, Ch, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Vo, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Am, Av, Ma, Sh, Bv, Ca, Ch, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Vo, Xp

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP1-30054-0.02ml

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NB600-450

Collagen I alpha 1 Antibody (COL-1)

(5 Reviews)

(68 Publications)

0.1 ml

NB600-450

<b>Biological Strategies Validation. </b>Collagen-I-alpha-1-Antibody-COL-1-Immunocytochemistry-Immunofluorescence-NB600-450-img0014.jpg

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary antibody. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and used at a dilution of 1:500.

Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-NB600-450-img0013.jpg

Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Mouse healthy liver. Anibody at 1:200. Enzymatic/proteolytic antigen retrieval. No permeabilization. IHC-P image submitted by a verified customer review.

(a) Histological section of keloid stained with picrosirius seen in ordinary light (20x). (b) Histological section of keloid stained with picrosirius seen in polarized light (20x). (c) Immunohistochemistry for type I collagen in keloid (20x). (d) Histological section of keloid stained with picrosirius seen in ordinary light (20x). (e) Histological section of keloid stained with picrosirius seen in polarized light (20x). (f) Immunohistochemistry for type III collagen in keloid (20x).

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - FSP‐1–positive cells only partially responsible for collagen I & III production in irradiated bladders. Bladder strips from irradiated mice were costained for collagen I or for collagen III mRNA (pink) & for fibroblasts (FSP‐1, brown). U: urothelium; LP: lamina propria. Black arrow: FSP‐1 positive cells. Gray arrow: collagen only positive cells. Scale bar = 50 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for collagen I or for collagen III and imaged using fluorescent microscopy. (a) Representative images (100 x 100 um) of each mouse strain and treatment group are provided for collagen I and collagen III immunofluorescence. (b–c) Percentage of tissue‐positive for collagen I or collagen III staining. Collagen I and III density is significantly elevated in C57BL/6 mice, but not in the C3H or BALB/c strains. Results are mean +/- SD of n = 3–6 mice. Dashed line: ANOVA; Full line: multiple t‐test. **p < .01, *** p < .001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, Rb

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF6220

Collagen I alpha 1 Antibody [Unconjugated]

(28 Publications)

100 μg

AF6220

HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human kidney tissue, human skin tissue, and human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human kidney tissue, human skin tissue, and human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF016). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of IMR‑90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Pro-Collagen I alpha 1 was detected in immersion fixed IMR-90 human lung fibroblast cell line using Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB6220) under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Human N-Pro Collagen I was immunoprecipitated from 500 μg of IMR‑90 human lung fibroblast cell line lysates with 5 μg of Sheep Anti-Human N-Pro Collagen I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220). The N-Pro Collagen I-antibody complexes were absorbed using Protein A or Protein G. Immunoprecipitated human N-Pro Collagen I was detected by Western blot using 1 μg/mL of Mouse Anti-Human N-Pro Collagen I Monoclonal Antibody (<a class=

MAB6220) under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of IMR‑90 human lung fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Collagen I alpha 1 at approximately 232 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Pro Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6220U-100UG
Alexa Fluor 405	AF6220V-100UG
Alexa Fluor 488	AF6220G-100UG
Alexa Fluor 532	AF6220X-100UG
Alexa Fluor 594	AF6220T-100UG
Alexa Fluor 647	AF6220R-100UG
Alexa Fluor 700	AF6220N-100UG
Alexa Fluor 750	AF6220S-100UG
Alexa Fluor Plus 405	AF6220AFP405-100UG
Alexa Fluor Plus 488	AF6220AFP488-100UG
Alexa Fluor Plus 555	AF6220AFP555-100UG
Alexa Fluor Plus 594	AF6220AFP594-100UG
Alexa Fluor Plus 647	AF6220AFP647-100UG
Alexa Fluor Plus 680	AF6220AFP680-100UG
Allophycocyanin	AF6220A
Biotin	AF6220B
CoraFluor 1	AF6220CL1
DyLight 350	AF6220D
DyLight 405	AF6220E
DyLight 488	AF6220K
DyLight 550	AF6220L
DyLight 594	AF6220M
DyLight 650	AF6220W
DyLight 680	AF6220Y
DyLight 755	AF6220Z
FITC	AF6220F
HRP	AF6220H
Janelia Fluor 525	AF6220JF525
Janelia Fluor 549	AF6220I
Janelia Fluor 646	AF6220J
Janelia Fluor 669	AF6220JF669
PE	AF6220P
PerCP	AF6220C
mFluor Violet 450 SE	AF6220MFV450
mFluor Violet 500 SE	AF6220MFV500
mFluor Violet 610 SE	AF6220MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6220-SP

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NBP1-77457

Collagen I alpha 1 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-77457

Reactivity:

Hu, Mu, Rt, Bv, Ca, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, Sh

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77457AF350
Alexa Fluor 405	NBP1-77457AF405
Alexa Fluor 488	NBP1-77457AF488
Alexa Fluor 532	NBP1-77457AF532
Alexa Fluor 594	NBP1-77457AF594
Alexa Fluor 647	NBP1-77457AF647
Alexa Fluor 700	NBP1-77457AF700
Alexa Fluor 750	NBP1-77457AF750
Alexa Fluor Plus 405	NBP1-77457AFP405
Alexa Fluor Plus 488	NBP1-77457AFP488
Alexa Fluor Plus 555	NBP1-77457AFP555
Alexa Fluor Plus 594	NBP1-77457AFP594
Alexa Fluor Plus 647	NBP1-77457AFP647
Alexa Fluor Plus 680	NBP1-77457AFP680
Allophycocyanin	NBP1-77457APC
Biotin	NBP1-77457B
CoraFluor 1	NBP1-77457CL1
DyLight 350	NBP1-77457UV
DyLight 405	NBP1-77457V
DyLight 488	NBP1-77457G
DyLight 550	NBP1-77457R
DyLight 594	NBP1-77457DL594
DyLight 650	NBP1-77457C
DyLight 680	NBP1-77457FR
DyLight 755	NBP1-77457IR
FITC	NBP1-77457F
HRP	NBP1-77457H
Janelia Fluor 525	NBP1-77457JF525
Janelia Fluor 549	NBP1-77457JF549
Janelia Fluor 646	NBP1-77457JF646
Janelia Fluor 669	NBP1-77457JF669
PE	NBP1-77457PE
PerCP	NBP1-77457PCP
mFluor Violet 450 SE	NBP1-77457MFV450
mFluor Violet 500 SE	NBP1-77457MFV500
mFluor Violet 610 SE	NBP1-77457MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77457SS

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NBP1-77458

Collagen I alpha 1 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-77458

Reactivity:

Hu, Mu, Po, Bt, Bv, Ca, Eq, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Po, Bt, Bv, Ca, Eq, Pm, Sh

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77458AF350
Alexa Fluor 405	NBP1-77458AF405
Alexa Fluor 488	NBP1-77458AF488
Alexa Fluor 532	NBP1-77458AF532
Alexa Fluor 594	NBP1-77458AF594
Alexa Fluor 647	NBP1-77458AF647
Alexa Fluor 700	NBP1-77458AF700
Alexa Fluor 750	NBP1-77458AF750
Alexa Fluor Plus 405	NBP1-77458AFP405
Alexa Fluor Plus 488	NBP1-77458AFP488
Alexa Fluor Plus 555	NBP1-77458AFP555
Alexa Fluor Plus 594	NBP1-77458AFP594
Alexa Fluor Plus 647	NBP1-77458AFP647
Alexa Fluor Plus 680	NBP1-77458AFP680
Allophycocyanin	NBP1-77458APC
Biotin	NBP1-77458B
CoraFluor 1	NBP1-77458CL1
DyLight 350	NBP1-77458UV
DyLight 405	NBP1-77458V
DyLight 488	NBP1-77458G
DyLight 550	NBP1-77458R
DyLight 594	NBP1-77458DL594
DyLight 650	NBP1-77458C
DyLight 680	NBP1-77458FR
DyLight 755	NBP1-77458IR
FITC	NBP1-77458F
HRP	NBP1-77458H
Janelia Fluor 525	NBP1-77458JF525
Janelia Fluor 549	NBP1-77458JF549
Janelia Fluor 646	NBP1-77458JF646
Janelia Fluor 669	NBP1-77458JF669
PE	NBP1-77458PE
PerCP	NBP1-77458PCP
mFluor Violet 450 SE	NBP1-77458MFV450
mFluor Violet 500 SE	NBP1-77458MFV500
mFluor Violet 610 SE	NBP1-77458MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77458SS

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MAB11700

Collagen I alpha 1 Antibody (1102924) [Unconjugated]

100 μg

MAB11700

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to connective tissue. Protocol available in COMET™ Panel Builder." class="big_lightbox">

COL1A1 was detected in immersion fixed paraffin-embedded sections of mouse Colon using Rat Anti-Mouse COL1A1, Monoclonal Antibody (Catalog #MAB11700) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to connective tissue. Protocol available in COMET™ Panel Builder." alt="COL1A1 was detected in immersion fixed paraffin-embedded sections of mouse Colon using Rat Anti-Mouse COL1A1, Monoclonal Antibody (Catalog #MAB11700) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to connective tissue. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Collagen I alpha 1 was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Collagen I alpha 1 was detected in perfusion fixed paraffin-embedded sections of mouse pancreas using Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Collagen I alpha 1 was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast adipose-like cell line (Positive) and absent in RAW 264.7 mouse monocyte/macrophage cell line (Negative) using Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF005). A specific band was detected for Collagen I alpha 1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for Collagen I alpha 1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF005) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of MEF mouse embryonic feeder cells, loaded at 0.1 mg/ml. A specific band was detected for Collagen I alpha 1 at approximately 226 kDa (as indicated) using 10 µg/ml of Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11700U-100UG
Alexa Fluor 405	FAB11700V-100UG
Alexa Fluor 488	FAB11700G-100UG
Alexa Fluor 532	FAB11700X-100UG
Alexa Fluor 594	FAB11700T-100UG
Alexa Fluor 647	FAB11700R-100UG
Alexa Fluor 700	FAB11700N-100UG
Alexa Fluor 750	FAB11700S-100UG
Alexa Fluor Plus 405	FAB11700AFP405-100UG
Alexa Fluor Plus 488	FAB11700AFP488-100UG
Alexa Fluor Plus 555	FAB11700AFP555-100UG
Alexa Fluor Plus 594	FAB11700AFP594-100UG
Alexa Fluor Plus 647	FAB11700AFP647-100UG
Alexa Fluor Plus 680	FAB11700AFP680-100UG
Allophycocyanin	FAB11700A
Biotin	FAB11700B
CoraFluor 1	FAB11700CL1
DyLight 350	FAB11700D
DyLight 405	FAB11700E
DyLight 488	FAB11700K
DyLight 550	FAB11700L
DyLight 594	FAB11700M
DyLight 650	FAB11700W
DyLight 680	FAB11700Y
DyLight 755	FAB11700Z
FITC	FAB11700F
HRP	FAB11700H
Janelia Fluor 525	FAB11700JF525
Janelia Fluor 549	FAB11700I
Janelia Fluor 646	FAB11700J
Janelia Fluor 669	FAB11700JF669
PE	FAB11700P
PerCP	FAB11700C
mFluor Violet 450 SE	FAB11700MFV450
mFluor Violet 500 SE	FAB11700MFV500
mFluor Violet 610 SE	FAB11700MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11700-SP

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MAB11757

Collagen I alpha 1 Antibody (3158C) [Unconjugated]

100 μg

MAB11757

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

Col1A1 was detected in immersion fixed paraffin-embedded sections of human Colon Cancer using Rabbit Anti-Human Col1A1, Monoclonal Antibody (Catalog # MAB) at 20ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." alt="Col1A1 was detected in immersion fixed paraffin-embedded sections of human Colon Cancer using Rabbit Anti-Human Col1A1, Monoclonal Antibody (Catalog # MAB) at 20ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

HAF008). A specific band was detected for Collagen I alpha 1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of Saos‑2 human osteosarcoma cell line and IMR‑90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11757) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band was detected for Collagen I alpha 1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Collagen I alpha 1 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Rabbit Anti-Human Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11757) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Collagen I alpha 1 was detected in immersion fixed paraffin-embedded sections of human cervix using Rabbit Anti-Human Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11757) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Collagen I alpha 1 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Rabbit Anti-Human Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11757) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Collagen I alpha 1 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11757) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11757U-100UG
Alexa Fluor 405	FAB11757V-100UG
Alexa Fluor 488	FAB11757G-100UG
Alexa Fluor 532	FAB11757X-100UG
Alexa Fluor 594	FAB11757T-100UG
Alexa Fluor 700	FAB11757N-100UG
Alexa Fluor 750	FAB11757S-100UG
Alexa Fluor Plus 405	FAB11757AFP405-100UG
Alexa Fluor Plus 488	FAB11757AFP488-100UG
Alexa Fluor Plus 555	FAB11757AFP555-100UG
Alexa Fluor Plus 594	FAB11757AFP594-100UG
Alexa Fluor Plus 647	FAB11757AFP647-100UG
Alexa Fluor Plus 680	FAB11757AFP680-100UG
Allophycocyanin	FAB11757A
Biotin	FAB11757B
CoraFluor 1	FAB11757CL1
DyLight 350	FAB11757D
DyLight 405	FAB11757E
DyLight 488	FAB11757K
DyLight 550	FAB11757L
DyLight 594	FAB11757M
DyLight 650	FAB11757W
DyLight 680	FAB11757Y
DyLight 755	FAB11757Z
FITC	FAB11757F
HRP	FAB11757H
Janelia Fluor 525	FAB11757JF525
Janelia Fluor 549	FAB11757I
Janelia Fluor 646	FAB11757J
Janelia Fluor 669	FAB11757JF669
PE	FAB11757P
PerCP	FAB11757C
mFluor Violet 450 SE	FAB11757MFV450
mFluor Violet 500 SE	FAB11757MFV500
mFluor Violet 610 SE	FAB11757MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11757-SP

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NBP2-92858

Collagen I alpha 1 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP2-92858

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92858] - Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using Collagen I alpha 1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-92858-0.02ml

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MAB6220

Collagen I alpha 1 Antibody (816161) [Unconjugated]

(1 Publication)

100 μg

MAB6220

HAF018). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of IMR‑90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human N-Pro Collagen I Monoclonal Antibody (Catalog # MAB6220) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of IMR‑90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human N-Pro Collagen I Monoclonal Antibody (Catalog # MAB6220) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Pro-Collagen I alpha 1 was detected in immersion fixed IMR-90 human lung fibroblast cell line using Mouse Anti-Human Pro-Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB6220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

AF6220) under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Human Pro-Collagen I alpha 1 was immunoprecipitated from 500 μg of IMR‑90 human lung fibroblast cell line lysates with 5 μg of Mouse Anti-Human Pro-Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB6220). The N-Pro Collagen I-antibody complexes were absorbed using Protein A or Protein G. Immunoprecipitated human Pro-Collagen I alpha 1 was detected by Western blot using 1 μg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (<a class=

AF6220) under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6220U-100UG
Alexa Fluor 405	FAB6220V-100UG
Alexa Fluor 488	FAB6220G-100UG
Alexa Fluor 532	FAB6220X-100UG
Alexa Fluor 594	FAB6220T-100UG
Alexa Fluor 647	FAB6220R-100UG
Alexa Fluor 700	FAB6220N-100UG
Alexa Fluor 750	FAB6220S-100UG
Alexa Fluor Plus 405	FAB6220AFP405-100UG
Alexa Fluor Plus 488	FAB6220AFP488-100UG
Alexa Fluor Plus 555	FAB6220AFP555-100UG
Alexa Fluor Plus 594	FAB6220AFP594-100UG
Alexa Fluor Plus 647	FAB6220AFP647-100UG
Alexa Fluor Plus 680	FAB6220AFP680-100UG
Allophycocyanin	FAB6220A
Biotin	FAB6220B
CoraFluor 1	FAB6220CL1
DyLight 350	FAB6220D
DyLight 405	FAB6220E
DyLight 488	FAB6220K
DyLight 550	FAB6220L
DyLight 594	FAB6220M
DyLight 650	FAB6220W
DyLight 680	FAB6220Y
DyLight 755	FAB6220Z
FITC	FAB6220F
HRP	FAB6220H
Janelia Fluor 525	FAB6220JF525
Janelia Fluor 549	FAB6220I
Janelia Fluor 646	FAB6220J
Janelia Fluor 669	FAB6220JF669
PE	FAB6220P
PerCP	FAB6220C
mFluor Violet 450 SE	FAB6220MFV450
mFluor Violet 500 SE	FAB6220MFV500
mFluor Violet 610 SE	FAB6220MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6220-SP

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NBP2-92877

Collagen I alpha 1 Antibody - BSA Free

0.1 ml

NBP2-92877

Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP2-92877] - Human colon using Collagen I/COL1A1 Rabbit pAb at dilution of 1:10000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92877] - Immunofluorescence analysis of NIH/3T3 cells using Collagen I alpha 1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-92877-0.02ml

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NBP1-68942

Collagen I alpha 1 Antibody - BSA Free

(1 Review)

(1 Publication)

100 ul

NBP1-68942

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-82488

Collagen I alpha 1 Antibody - BSA Free

0.1 ml

NBP1-82488

Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP1-82488] - Staining of human cervix shows moderate cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP1-82488] - Staining of human colon shows moderate cytoplasmic positivity in stromal cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-82488-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Collagen I alpha 1 Antibodies

Collagen I alpha 1 Antibody - Azide and BSA Free

Collagen I alpha 1 Antibody (COL-1)

Collagen I alpha 1 Antibody [Unconjugated]

Collagen I alpha 1 Antibody - BSA Free

Collagen I alpha 1 Antibody - BSA Free

Collagen I alpha 1 Antibody (1102924) [Unconjugated]

Collagen I alpha 1 Antibody (3158C) [Unconjugated]

Collagen I alpha 1 Antibody - BSA Free

Collagen I alpha 1 Antibody (816161) [Unconjugated]

Collagen I alpha 1 Antibody - BSA Free

Collagen I alpha 1 Antibody - BSA Free

Collagen I alpha 1 Antibody - BSA Free