Western Blot: Collagen II Antibody (5B2.5) [NB600-844] - Expression levels of related genes during differentiation induction in the 2D environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using ...read more
Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33120203).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified
Alternate Names for Collagen II Antibody (5B2.5) - BSA Free
Alpha-1 type II collagen
ANFH
AOM
cartilage collagen
Chondrocalcin
COL11A3
COL2A1
Collagen 2
collagen alpha-1(II) chain
Collagen II
collagen II, alpha-1 polypeptide
collagen type 2
collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
collagen, type II, alpha 1
Collagen-2
MGC131516
SEDC
Background
Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Wang X, Lu Y, Wang W et al. Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo Regenerative Biomaterials Aug 4 2020 (IHC, Rabbit)
Yu H, Liu Y, Yang X et al. Strontium Ranelate Promotes Chondrogenesis Through Inhibition of the Wnt/beta-catenin Pathway Research Square Mar 23 2021 (ICC/IF, Rat)
Meng X, Grad S, Wen C et al. An impaired healing model of osteochondral defect in papain-induced arthritis Journal of Orthopaedic Translation Sep 1 2020 (IHC, Rabbit)
IHC
Rabbit
He W, Wang W, Deng J et al. L-NMMA AND 1400W, INHIBITORS OF iNOS, ATTENUATE THE INDUCTION OF iNOS AND NO IN PRIMARY RABBIT COSTAL CHONDROCYTES BY FLUORIDE Fluoride Jan 1 2020 (WB, Rabbit)
Dong Y, Sun X, Zhang Z et al. Regional and sustained dual-release of growth factors from biomimetic tri-layered scaffolds for the repair of large-scale osteochondral defects Applied Materials Today Jun 1 2020 (IHC)
IHC
Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon Nov 1 2019 (IHC, Human)
Bioinformatics Tool for Collagen II Antibody (NB600-844)
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Diseases for Collagen II Antibody (NB600-844)
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