Collagen II Antibody (5B2.5)

Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after inoculation onto slides. Image collected and cropped by Cite Ab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

• Reactivity: Hu, Mu, Rt, Ch, Rb
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 5B2.5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Collagen II Antibody (5B2.5) Summary

• Immunogen: A purified preparation of lathyritic type II collagen from embryonic chicken sternum
• Localization: Cartilage matrix
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL2A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:2000

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33120203).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Collagen II Antibody (5B2.5)

• Alpha-1 type II collagen
• ANFH
• AOM
• cartilage collagen
• Chondrocalcin
• COL11A3
• COL2A1
• Collagen 2
• collagen alpha-1(II) chain
• Collagen II
• collagen II, alpha-1 polypeptide
• collagen type 2
• collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
• collagen, type II, alpha 1
• Collagen-2
• MGC131516
• SEDC

Background

Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen II Antibody (NB600-844)(21)

Publications using NB600-844

• Jing H, Zhang X, Luo K, et al. miR-381-abundant small extracellular vesicles derived from kartogenin-preconditioned mesenchymal stem cells promote chondrogenesis of MSCs by targeting TAOK1 Biomaterials Feb 1 2020 [PMID: 31864017] (WB, Human)
• Chen L, Liu G, Li W, Wu X. Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system Cell Mol Biol Lett Dec 1 2019 [PMID: 30858866] (WB, Rabbit)
• Chen Y, Sui J, Wang Q et al. Injectable self-crosslinking HA-SH/Col I blend hydrogels for in vitro construction of engineered cartilage Carbohydr Polym 2018 Jun 15 [PMID: 29628260] (IHC, Rabbit)
• Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015 Aug 03 [PMID: 26230691] (WB, Chicken)
  
  Details:
  Collagen II antibody (clone 5B2.5) was used at 1:100 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.
• Wang X, Lu Y, Wang W et al. Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo Regenerative Biomaterials Aug 4 2020 (IHC, Rabbit)
• Meng X, Grad S, Wen C et al. An impaired healing model of osteochondral defect in papain-induced arthritis Journal of Orthopaedic Translation Sep 1 2020 (IHC, Rabbit)
• He W, Wang W, Deng J et al. L-NMMA AND 1400W, INHIBITORS OF iNOS, ATTENUATE THE INDUCTION OF iNOS AND NO IN PRIMARY RABBIT COSTAL CHONDROCYTES BY FLUORIDE Fluoride Jan 1 2020 (WB, Rabbit)
• Qiao Z, Lian M, Han Y et al. Bioinspired stratified electrowritten fiber-reinforced hydrogel constructs with layer-specific induction capacity for functional osteochondral regeneration Biomaterials Oct 17 2020 [PMID: 33120203] (WB, Rabbit)
• Yan L, Wu X Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity Cell Biol. Toxicol. Dec 9 2019 [PMID: 31820164] (WB, Human)
• Tome-Garcia J, Erfani P et al. Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma. Nat Commun Jan 10 2018 [PMID: 30275445] (WB, Human)
• Dong Y, Sun X, Zhang Z et al. Regional and sustained dual-release of growth factors from biomimetic tri-layered scaffolds for the repair of large-scale osteochondral defects Applied Materials Today Jun 1 2020 (IHC)
• Triulzi T, Ratti M, Tortoreto M et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2013 Nov 14 [PMID: 24242003] (IHC-P, Human)
• Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon Nov 1 2019 (IHC, Human)
• You Q, Liu Z, Zhang J et al. Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects Am J Sports Med Jan 15 2020 [PMID: 31940211]
• Cheng B, Liu Y, Zhao Y et al. The role of anthrax toxin protein receptor 1 as a new mechanosensor molecule and its mechanotransduction in BMSCs under hydrostatic pressure Sci Rep Sep 2 2019 [PMID: 31477767] (WB, Rat)
• Suo H, Li L, Zhang C et al. Glucosamine-grafted methacrylated gelatin hydrogels as potential biomaterials for cartilage repair J. Biomed. Mater. Res. Part B Appl. Biomater. Aug 1 2019 [PMID: 31369700]
• Gao B, Jing H, Gao M et al. Long-segmental Tracheal Reconstruction in Rabbits with Pedicled Tissue-engineered Trachea based on a 3D-printed Scaffold Acta Biomater Jul 25 2019 [PMID: 31352107] (IHC-P, Rabbit)
• Zhu L, Weng Z, Shen P et al. S100B regulates inflammatory response during osteoarthritis via fibroblast growth factor receptor 1 signaling. Mol Med Rep. Oct 1 2018 [PMID: 30280200] (IHC, Human)
• Chen Honglin, Malheiro Afonso de Botelho Ferreira Braga, Van Blitterswijk Clemens et al. Direct Writing Electrospinning of Scaffolds with Multidimensional Fiber Architecture for Hierarchical Tissue Engineering. ACS Omega. 2017 Nov 8 [PMID: 29043781] (IHC-P, Human)
• Brini AT, Niada S, Lambertini E et al. Chondrogenic potential of Human mesenchymal stem cells and expression of Slug transcription factor. J Tissue Eng Regen Med 2013 Jul 21 [PMID: 23873763]
• Dahia CL, Mahoney E, Wylie C. Shh signaling from the nucleus pulposus is required for the postnatal growth and differentiation of the mouse intervertebral disc. PLoS One. 20127(4). [PMID: 22558278] (IHC-Fr, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: COL2A1
• Entrez:
  • 1280(Human)
  • 12824(Mouse)
  • 395069(Chicken)
• Uniprot:
  • Q90W37(Chicken)