Succesful use in IHC has been reported for previous lots in scientific literature (PMID: 22558278; 24242003). Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS (pH 7.4) and 0.2% BSA
0.09% Sodium Azide
Protein A purified
Alternate Names for Collagen II Antibody (5B2.5)
Alpha-1 type II collagen
collagen alpha-1(II) chain
collagen II, alpha-1 polypeptide
collagen type 2
collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
collagen, type II, alpha 1
Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Collagen II Antibody (NB600-844). (Showing 1 - 1 of 1 FAQs).
We would like to know whether this ab could be used in IHC, could you please advise?
This antibody has not yet been validated in IHC by our lab. Only in IF and WB. The IF was run on cells and not tissues. Most likely though if the antibody stains cells there is a good chance it will also work in tissue, but we cannot guarantee this. We can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.
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