Collagen II Antibody (5B2.5)

Images

 
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
5B2.5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Collagen II Antibody (5B2.5) Summary

Immunogen
A purified preparation of lathyritic type II collagen from embryonic chicken sternum
Localization
Cartilage matrix
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL2A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:2000
Publications
Read Publications using
NB600-844 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33120203).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Collagen II Antibody (5B2.5)

  • Alpha-1 type II collagen
  • ANFH
  • AOM
  • cartilage collagen
  • Chondrocalcin
  • COL11A3
  • COL2A1
  • Collagen 2
  • collagen alpha-1(II) chain
  • Collagen II
  • collagen II, alpha-1 polypeptide
  • collagen type 2
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
  • collagen, type II, alpha 1
  • Collagen-2
  • MGC131516
  • SEDC

Background

Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen II Antibody (NB600-844)(21)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Rabbit.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(6)
IHC-Fr
(1)
IHC-P
(3)
WB
(8)
All Applications
Filter By Species
Human
(7)
Mouse
(1)
Rat
(1)
Chicken
(1)
Rabbit
(7)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB600-844 Applications Species
Jing H, Zhang X, Luo K, et al. miR-381-abundant small extracellular vesicles derived from kartogenin-preconditioned mesenchymal stem cells promote chondrogenesis of MSCs by targeting TAOK1 Biomaterials Feb 1 2020 [PMID: 31864017] (WB, Human) WB Human
Chen L, Liu G, Li W, Wu X. Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system Cell Mol Biol Lett Dec 1 2019 [PMID: 30858866] (WB, Rabbit) WB Rabbit
Chen Y, Sui J, Wang Q et al. Injectable self-crosslinking HA-SH/Col I blend hydrogels for in vitro construction of engineered cartilage Carbohydr Polym 2018 Jun 15 [PMID: 29628260] (IHC, Rabbit) IHC Rabbit
Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015 Aug 03 [PMID: 26230691] (WB, Chicken)

Details:
Collagen II antibody (clone 5B2.5) was used at 1:100 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.
WB Chicken
Wang X, Lu Y, Wang W et al. Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo Regenerative Biomaterials Aug 4 2020 (IHC, Rabbit) IHC Rabbit
Meng X, Grad S, Wen C et al. An impaired healing model of osteochondral defect in papain-induced arthritis Journal of Orthopaedic Translation Sep 1 2020 (IHC, Rabbit) IHC Rabbit
He W, Wang W, Deng J et al. L-NMMA AND 1400W, INHIBITORS OF iNOS, ATTENUATE THE INDUCTION OF iNOS AND NO IN PRIMARY RABBIT COSTAL CHONDROCYTES BY FLUORIDE Fluoride Jan 1 2020 (WB, Rabbit) WB Rabbit
Qiao Z, Lian M, Han Y et al. Bioinspired stratified electrowritten fiber-reinforced hydrogel constructs with layer-specific induction capacity for functional osteochondral regeneration Biomaterials Oct 17 2020 [PMID: 33120203] (WB, Rabbit) WB Rabbit
Yan L, Wu X Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity Cell Biol. Toxicol. Dec 9 2019 [PMID: 31820164] (WB, Human) WB Human
Tome-Garcia J, Erfani P et al. Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma. Nat Commun Jan 10 2018 [PMID: 30275445] (WB, Human) WB Human
Show All 21 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen II Antibody (NB600-844) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-844AF350
Alexa Fluor 405 NB600-844AF405
Alexa Fluor 488 NB600-844AF488
Alexa Fluor 532 NB600-844AF532
Alexa Fluor 594 NB600-844AF594
Alexa Fluor 647 NB600-844AF647
Alexa Fluor 700 NB600-844AF700
Alexa Fluor 750 NB600-844AF750
Biotin NB600-844B
DyLight 350 NB600-844UV
DyLight 405 NB600-844V
DyLight 488 NB600-844G
DyLight 550 NB600-844R
DyLight 594 NB600-844DL594
DyLight 650 NB600-844C
DyLight 680 NB600-844FR
DyLight 755 NB600-844IR
FITC NB600-844F
HRP NB600-844H
Janelia Fluor 549 NB600-844JF549
Janelia Fluor 646 NB600-844JF646

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Bioinformatics

Gene Symbol COL2A1
Entrez
Uniprot