Collagen II Antibody (5B2.5) - BSA Free

Images

 
Western Blot: Collagen II Antibody (5B2.5) [NB600-844] - Expression levels of related genes during differentiation induction in the 2D environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
5B2.5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Collagen II Antibody (5B2.5) - BSA Free Summary

Immunogen
A purified preparation of lathyritic type II collagen from embryonic chicken sternum
Localization
Cartilage matrix
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL2A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:2000
  • Western Blot 1-2 ug/ml
Publications
Read Publications using
NB600-844 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33120203).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Collagen II Antibody (5B2.5) - BSA Free

  • Alpha-1 type II collagen
  • ANFH
  • AOM
  • cartilage collagen
  • Chondrocalcin
  • COL11A3
  • COL2A1
  • Collagen 2
  • collagen alpha-1(II) chain
  • Collagen II
  • collagen II, alpha-1 polypeptide
  • collagen type 2
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
  • collagen, type II, alpha 1
  • Collagen-2
  • MGC131516
  • SEDC

Background

Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen II Antibody (NB600-844)(32)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Rabbit.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(7)
IHC-Fr
(1)
IHC-P
(6)
WB
(10)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
Rat
(3)
Chicken
(1)
Rabbit
(12)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB600-844 Applications Species
Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015 Aug 03 [PMID: 26230691] (WB, Chicken)

Details:
Collagen II antibody (clone 5B2.5) was used at 1:100 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.
WB Chicken
Wang X, Lu Y, Wang W et al. Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo Regenerative Biomaterials Aug 4 2020 (IHC, Rabbit) IHC Rabbit
Li L, Chen Y, Fu Q Et al. Decellularized extracellular matrix loaded with IPFP-SC for repairing rabbit osteochondral defects American journal of translational research Oct 15 2021 [PMID: 34786041] (ICC/IF, Rabbit) ICC/IF Rabbit
Wang J, Huang L, Yang X Et al. The Regulatory Effect of MicroRNA-101-3p on Disc Degeneration by the STC1/VEGF/MAPK Pathway Oxidative medicine and cellular longevity Oct 5 2021 [PMID: 34650661] (WB, Human) WB Human
Zhang N, Wang Y, Zhang J Et al. Controlled domain gels with a biomimetic gradient environment for osteochondral tissue regeneration Acta biomaterialia Aug 25 2021 [PMID: 34454084] (IHC-P, Rabbit) IHC-P Rabbit
Zhang W, Wang H, Yuan Z et al. Moderate mechanical stimulation rescues degenerative annulus fibrosus by suppressing caveolin-1 mediated pro-inflammatory signaling pathway International journal of biological sciences Apr 3 2021 [PMID: 33867854] (ICC/IF, Rat) ICC/IF Rat
Jung H, Mclellan P, Welter JF, Akkus O Chondrogenesis Mesenchymal Stem Cells via Local Release of TGF beta 3 from Heparinized Collagen Biofabric Tissue engineering. Part A Apr 7 2021 [PMID: 33827271]
Yu H, Liu Y, Yang X et al. Strontium Ranelate Promotes Chondrogenesis Through Inhibition of the Wnt/beta-catenin Pathway Research Square Mar 23 2021 (ICC/IF, Rat) ICC/IF Rat
Yan L, Liu G, Wu X The umbilical cord mesenchymal stem cell-derived exosomal lncRNA H19 improves osteochondral activity through miR-29b-3p/FoxO3 axis Clinical and translational medicine Jan 1 2021 [PMID: 33463060] (WB) WB
Show All 32 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COL2A1
Entrez
Uniprot