Western Blot: Collagen II Antibody (5B2.5) [NB600-844] - Expression levels of related genes during differentiation induction in the 2D environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using ...read more
Use in Rabbit reported in scientific literature (PMID:35441114).Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33120203).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
0.02% Sodium Azide
Protein A purified
Alternate Names for Collagen II Antibody (5B2.5) - BSA Free
Alpha-1 type II collagen
collagen alpha-1(II) chain
collagen II, alpha-1 polypeptide
collagen type 2
collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
collagen, type II, alpha 1
Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019-11-01 (IF/IHC, Human)
Luo Z, Liu Z, Yu H et al. Multifunctional mesoporous polydopamine near-infrared photothermal controlled release of kartogenin for cartilage repair Materials & Design 2023-07-01
Zheng Z, Xu J, Tu Z et al. Promoter DNA Hypermethylation of Latexin Promotes Inflammatory Activation of Fibro-Nucleus Pulposus Cells in Intervertebral Disc Degeneration papers.ssrn.com 2023-05-22 (WB, Human)
Dong Y, Sun X, Zhang Z et al. Regional and sustained dual-release of growth factors from biomimetic tri-layered scaffolds for the repair of large-scale osteochondral defects Applied Materials Today 2020-06-01 (IF/IHC)
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