Collagen II Antibody (5B2.5)

Immunohistochemistry: Collagen II Antibody (5B2.5) [NB600-844] - IHC analysis of a formalin fixed paraffin embedded section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

• Reactivity: Hu, Mu, Rt, Ch, Rb
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: 5B2.5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Collagen II Antibody (5B2.5) Summary

• Immunogen: A purified preparation of lathyritic type II collagen from embryonic chicken sternum
• Localization: Cartilage matrix
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL2A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: IHC-P usage was reported in scientific literature (PMID: 24242003). WB usage was reported in scientific literature.

Reactivity Notes

Succesful use in IHC has been reported for previous lots in scientific literature (PMID: 22558278; 24242003). Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Collagen II Antibody (5B2.5)

• Alpha-1 type II collagen
• ANFH
• AOM
• cartilage collagen
• Chondrocalcin
• COL11A3
• COL2A1
• Collagen 2
• collagen alpha-1(II) chain
• Collagen II
• collagen II, alpha-1 polypeptide
• collagen type 2
• collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
• collagen, type II, alpha 1
• Collagen-2
• MGC131516
• SEDC

Background

Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen II Antibody (NB600-844)(5)

Publications using NB600-844

• Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015 Aug 03 [PMID: 26230691] (WB, Chicken)
  
  Details:
  Collagen II antibody (clone 5B2.5) was used at 1:100 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.
• Lekawanvijit S, Kumfu S, Wang BH et al. The Uremic Toxin Adsorbent AST-120 Abrogates Cardiorenal Injury Following Myocardial Infarction. PLoS One 2013 Dec 13 [PMID: 24349545] (WB, Rat)
• Triulzi T, Ratti M, Tortoreto M et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2013 Nov 14 [PMID: 24242003] (IHC-P, Human)
• Brini AT, Niada S, Lambertini E et al. Chondrogenic potential of Human mesenchymal stem cells and expression of Slug transcription factor. J Tissue Eng Regen Med 2013 Jul 21 [PMID: 23873763]
• Dahia CL, Mahoney E, Wylie C. Shh signaling from the nucleus pulposus is required for the postnatal growth and differentiation of the mouse intervertebral disc. PLoS One. 20127(4). [PMID: 22558278] (IHC-Fr, ICC/IF, Mouse)

FAQs for Collagen II Antibody (NB600-844). (Showing 1 - 1 of 1 FAQs).

1. We would like to know whether this ab could be used in IHC, could you please advise?
   • This antibody has not yet been validated in IHC by our lab. Only in IF and WB. The IF was run on cells and not tissues. Most likely though if the antibody stains cells there is a good chance it will also work in tissue, but we cannot guarantee this. We can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.

Bioinformatics

• Gene Symbol: COL2A1
• Entrez:
  • 1280(Human)
  • 12824(Mouse)
  • 25412(Rat)
  • 395069(Chicken)
• Uniprot:
  • Q90W37(Chicken)