Collagen II Antibody (5B2.5)

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Immunohistochemistry: Collagen II Antibody (5B2.5) [NB600-844] - IHC analysis of a formalin fixed paraffin embedded section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
5B2.5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Collagen II Antibody (5B2.5) Summary

Immunogen
A purified preparation of lathyritic type II collagen from embryonic chicken sternum
Localization
Cartilage matrix
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL2A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
Application Notes
IHC-P usage was reported in scientific literature (PMID: 24242003). WB usage was reported in scientific literature.
Publications
Read Publications using
NB600-844 in the following applications:

Reactivity Notes

Succesful use in IHC has been reported for previous lots in scientific literature (PMID: 22558278; 24242003). Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Collagen II Antibody (5B2.5)

  • Alpha-1 type II collagen
  • ANFH
  • AOM
  • cartilage collagen
  • Chondrocalcin
  • COL11A3
  • COL2A1
  • Collagen 2
  • collagen alpha-1(II) chain
  • Collagen II
  • collagen II, alpha-1 polypeptide
  • collagen type 2
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
  • collagen, type II, alpha 1
  • Collagen-2
  • MGC131516
  • SEDC

Background

Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen II Antibody (NB600-844)(5)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 4 applications: ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(2)
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Filter By Species
Human
(1)
Mouse
(1)
Rat
(1)
Chicken
(1)
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Showing Publications 1 - 5 of 5.
Publications using NB600-844 Applications Species
Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015 Aug 03 [PMID: 26230691] (WB, Chicken)

Details:
Collagen II antibody (clone 5B2.5) was used at 1:100 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.
WB Chicken
Lekawanvijit S, Kumfu S, Wang BH et al. The Uremic Toxin Adsorbent AST-120 Abrogates Cardiorenal Injury Following Myocardial Infarction. PLoS One 2013 Dec 13 [PMID: 24349545] (WB, Rat) WB Rat
Triulzi T, Ratti M, Tortoreto M et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2013 Nov 14 [PMID: 24242003] (IHC-P, Human) IHC-P Human
Brini AT, Niada S, Lambertini E et al. Chondrogenic potential of Human mesenchymal stem cells and expression of Slug transcription factor. J Tissue Eng Regen Med 2013 Jul 21 [PMID: 23873763]
Dahia CL, Mahoney E, Wylie C. Shh signaling from the nucleus pulposus is required for the postnatal growth and differentiation of the mouse intervertebral disc. PLoS One. 20127(4). [PMID: 22558278] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen II Antibody (NB600-844). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know whether this ab could be used in IHC, could you please advise?
    • This antibody has not yet been validated in IHC by our lab. Only in IF and WB. The IF was run on cells and not tissues. Most likely though if the antibody stains cells there is a good chance it will also work in tissue, but we cannot guarantee this. We can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.

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Bioinformatics

Gene Symbol COL2A1
Entrez
Uniprot