Recombinant Human/Mouse/Rat BMP-2 Protein


Recombinant human/mouse/rat BMP-2 (355-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 40-200 ng/mL.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity

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Recombinant Human/Mouse/Rat BMP-2 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived BMP-2 protein
Accession #
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
15-16 kDa, reducing conditions
Read Publications using
355-BM in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid with BSA as a carrier protein.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat BMP-2 Protein

  • BDA2
  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2


Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP‑2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR‑IA, or BMPR‑IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).
  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Wozney, J. et al. (1988) Science 242:1528.
  4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
  5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
  6. De Luca, F. et al. (2001) Endocrinology 142:430.
  7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
  8. Ryoo, H.-M. et al. (2006) Gene 366:51.
  9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
  10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
  11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
  13. Kano, Y. et al. (2005) Endocrinology 146:5332.
  14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
  15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

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Publications for BMP-2 (355-BM)(185)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, Hamster, N/A, Phallusia mammillata, Porcine, Rabbit, Rats, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, ELISA (Capture), ELISA Capture, In Vivo, Organoid Culture, Surface Plasmon Resonance.

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Phallusia mammillata
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Showing Publications 1 - 10 of 185. Show All 185 Publications.
Publications using 355-BM Applications Species
Erdem, C;Gross, SM;Heiser, LM;Birtwistle, MR; MOBILE pipeline enables identification of context-specific networks and regulatory mechanisms Nature communications 2023-07-06 [PMID: 37414767] (Bioassay, Human) Bioassay Human
Nagasaki, K;Yamada, A;Sasa, K;Kamijo, R; Kielin/chordin-like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein-2 FEBS open bio 2023-05-25 [PMID: 37226930] (Differentiation, Differentiation, Mouse) Differentiation, Differentiation Mouse
S Das, A Mondal, C Dey, S Chakrabort, R Bhowmik, S Karmakar, A Sengupta ER stress induces upregulation of transcription factor Tbx20 and downstream Bmp2 signaling to promote cardiomyocyte survival The Journal of Biological Chemistry, 2023-02-16;0(0):103031. 2023-02-16 [PMID: 36805334] (Bioassay, Rat) Bioassay Rat
Y Hachinohe, M Taira, M Hoshi, D Yoshida, W Hatakeyama, T Sawada, H Kondo Self-Prepared Hyaluronic Acid/Alkaline Gelatin Composite with Nano-Hydroxyapatite and Bone Morphogenetic Protein for Cranial Bone Formation International Journal of Molecular Sciences, 2023-01-06;24(2):. 2023-01-06 [PMID: 36674618] (In Vivo, Rat) In Vivo Rat
Y Hachinohe, M Taira, M Hoshi, W Hatakeyama, T Sawada, H Kondo Bone Formation on Murine Cranial Bone by Injectable Cross-Linked Hyaluronic Acid Containing Nano-Hydroxyapatite and Bone Morphogenetic Protein Polymers, 2022-12-08;14(24):. 2022-12-08 [PMID: 36559734] (In Vivo, Mouse) In Vivo Mouse
Z Shonibare, M Monavarian, K O'Connell, D Altomare, A Shelton, S Mehta, R Jaskula-Sz, R Phaeton, MD Starr, R Whitaker, A Berchuck, AB Nixon, RC Arend, NY Lee, CR Miller, N Hempel, K Mythreye Reciprocal SOX2 regulation by SMAD1-SMAD3 is critical for anoikis resistance and metastasis in cancer Oncogene, 2022-07-26;40(4):111066. 2022-07-26 [PMID: 35905726] (Bioassay, Human) Bioassay Human
R Chowdhury, A Roure, Y le Pétillo, H Mayeur, V Daric, S Darras Highly distinct genetic programs for peripheral nervous system formation in chordates Bmc Biology, 2022-06-27;20(1):152. 2022-06-27 [PMID: 35761237] (Bioassay, Phallusia mammillata) Bioassay Phallusia mammillata
M Vora, A Mondal, D Jia, P Gaddipati, M Akel, J Gilleran, J Roberge, C Rongo, J Langenfeld Bone morphogenetic protein signaling regulation of AMPK and PI3K in lung cancer cells and C. elegans Cell & bioscience, 2022-05-31;12(1):76. 2022-05-31 [PMID: 35641992] (Bioassay, Human) Bioassay Human
AP Leinroth, AJ Mirando, D Rouse, Y Kobayahsi, PR Tata, HE Rueckert, Y Liao, JT Long, JV Chakkalaka, MJ Hilton Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations Cell Reports, 2022-05-10;39(6):110785. 2022-05-10 [PMID: 35545045] (Bioassay, Mouse) Bioassay Mouse
F Kawecki, J Jann, M Fortin, FA Auger, N Faucheux, J Fradette Preclinical Evaluation of BMP-9-Treated Human Bone-like Substitutes for Alveolar Ridge Preservation following Tooth Extraction International Journal of Molecular Sciences, 2022-03-18;23(6):. 2022-03-18 [PMID: 35328724] (Cell Culture, Human) Cell Culture Human
Show All 185 Publications.

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FAQs for BMP-2 (355-BM). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

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Gene Symbol BMP2