Recombinant Human/Mouse/Rat BMP-2 Protein

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity

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Recombinant Human/Mouse/Rat BMP-2 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.
The specific activity of Recombinant Human BMP-2 is approximately 781 U/μg, which is calibrated against human BMP-2 WHO International Standard (NIBSC code: 93/574).
Source
Chinese Hamster Ovary cell line, CHO-derived BMP-2 protein
Gln283-Arg396
Accession #
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
15-16 kDa, reducing conditions
Publications
Read Publications using
355-BM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat BMP-2 Protein

  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2

Background

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP‑2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR‑IA, or BMPR‑IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).
  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Wozney, J. et al. (1988) Science 242:1528.
  4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
  5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
  6. De Luca, F. et al. (2001) Endocrinology 142:430.
  7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
  8. Ryoo, H.-M. et al. (2006) Gene 366:51.
  9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
  10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
  11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
  13. Kano, Y. et al. (2005) Endocrinology 146:5332.
  14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
  15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

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355-BM
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Applications: Bioactivity

Publications for BMP-2 (355-BM)(116)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, Rabbit, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, ELISA capture, In Ovo, In Vivo, In Vivo grafting, Organogenesis, Surface Plasmon Resonance, complex formation.


Filter By Application
Binding Assay
(1)
Bioassay
(101)
ELISA capture
(1)
In Ovo
(1)
In Vivo
(5)
In Vivo grafting
(1)
Organogenesis
(1)
Surface Plasmon Resonance
(2)
complex formation
(1)
All Applications
Filter By Species
Human
(43)
Mouse
(51)
Rat
(6)
Bovine
(3)
Canine
(1)
Chicken
(3)
Equine
(1)
Rabbit
(2)
Transgenic Mouse
(1)
Xenopus
(3)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 116. Show All 116 Publications.
Publications using 355-BM Applications Species
H Kang, S Jha, Z Deng, N Fratzl-Zel, WA Cabral, A Ivovic, F Meylan, EP Hanson, E Lange, J Katz, P Roschger, K Klaushofer, EW Cowen, RM Siegel, JC Marini, T Bhattachar Somatic activating mutations in MAP2K1 cause melorheostosis Nat Commun, 2018;9(1):1390. 2018 [PMID: 29643386] (Bioassay, Human) Bioassay Human
M Desancé, R Contentin, L Bertoni, T Gomez-Ledu, T Branly, S Jacquet, JM Betsch, A Batho, F Legendre, F Audigié, P Galéra, M Demoor Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy Int J Mol Sci, 2018;19(2):. 2018 [PMID: 29439436] (Bioassay, Equine) Bioassay Equine
S Kokabu, V Rosen BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling FEBS Open Bio, 2018;8(2):168-176. 2018 [PMID: 29435407] (Bioassay, Mouse) Bioassay Mouse
JB Lees-Shepa, M Yamamoto, AA Biswas, SJ Stoessel, SE Nicholas, CA Cogswell, PM Devarakond, MJ Schneider, SM Cummins, NP Legendre, S Yamamoto, V Kaartinen, JW Hunter, DJ Goldhamer Activin-dependent signaling in fibro/adipogenic progenitors causes fibrodysplasia ossificans progressiva Nat Commun, 2018;9(1):471. 2018 [PMID: 29396429] (In Vivo, Mouse) In Vivo Mouse
WS Fagg, N Liu, MJ Yang, K Cheng, E Chung, JS Kim, G Wu, J Fair Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver Cell Transplant, 2017;26(12):1868-1877. 2017 [PMID: 29390880] (Bioassay, Mouse) Bioassay Mouse
MG Perino, S Yamanaka, DR Riordon, Y Tarasova, KR Boheler Ascorbic acid promotes cardiomyogenesis through SMAD1 signaling in differentiating mouse embryonic stem cells PLoS ONE, 2017;12(12):e0188569. 2017 [PMID: 29232368] (Bioassay, Mouse) Bioassay Mouse
Y Yu, S Gu, W Li, C Sun, F Chen, M Xiao, L Wang, D Xu, Y Li, C Ding, Z Xia, Y Li, S Ye, P Xu, B Zhao, J Qin, YG Chen, X Lin, XH Feng Smad7 enables STAT3 activation and promotes pluripotency independent of TGF-? signaling Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28874583] (Bioassay, Mouse) Bioassay Mouse
SJ Bae, HJ Kim, HY Won, YK Min, ES Hwang Acceleration of osteoblast differentiation by a novel osteogenic compound, DMP-PYT, through activation of both the BMP and Wnt pathways Sci Rep, 2017;7(1):8455. 2017 [PMID: 28814721] (Bioassay, Mouse) Bioassay Mouse
J Fan, J Pi-Anfruns, M Guo, DCS Im, ZK Cui, S Kim, BM Wu, TL Aghaloo, M Lee Small molecule-mediated tribbles homolog 3 promotes bone formation induced by bone morphogenetic protein-2 Sci Rep, 2017;7(1):7518. 2017 [PMID: 28790361] (Bioassay, Human) Bioassay Human
C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28747434] (Bioassay, Human) Bioassay Human
Show All 116 Publications.

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FAQs for BMP-2 (355-BM). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

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Bioinformatics

Gene Symbol BMP2
Entrez
Uniprot