Recombinant Human/Mouse/Rat BMP-2 Protein

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human/Mouse/Rat BMP-2 Protein Summary

Additional Information
More economical E. coli option available
Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.
The specific activity of Recombinant Human BMP-2 is approximately 781 U/μg, which is calibrated against human BMP-2 WHO International Standard (NIBSC code: 93/574).
Source
Chinese Hamster Ovary cell line, CHO-derived BMP-2 protein
Gln283-Arg396
Accession #
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
15-16 kDa, reducing conditions
Publications
Read Publications using
355-BM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat BMP-2 Protein

  • BDA2
  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2
  • SSFSC

Background

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP‑2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR‑IA, or BMPR‑IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).
  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Wozney, J. et al. (1988) Science 242:1528.
  4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
  5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
  6. De Luca, F. et al. (2001) Endocrinology 142:430.
  7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
  8. Ryoo, H.-M. et al. (2006) Gene 366:51.
  9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
  10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
  11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
  13. Kano, Y. et al. (2005) Endocrinology 146:5332.
  14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
  15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

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Publications for BMP-2 (355-BM)(150)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, Porcine, Rabbit, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), In Ovo, In Vivo, In vivo, Organoid Culture, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(2)
Bioassay
(127)
Cell Culture
(5)
ELISA (Capture)
(1)
In Ovo
(1)
In Vivo
(8)
In vivo
(1)
Organoid Culture
(1)
Surface Plasmon Resonance
(2)
All Applications
Filter By Species
Human
(56)
Mouse
(68)
Rat
(7)
Bovine
(3)
Canine
(1)
Chicken
(4)
Equine
(1)
Porcine
(2)
Rabbit
(2)
Transgenic Mouse
(1)
Xenopus
(3)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 150. Show All 150 Publications.
Publications using 355-BM Applications Species
S Qin, W Wang, Z Liu, X Hua, S Fu, F Dong, A Li, Z Liu, P Wang, L Dai, P Liang, J Zhang, W Cao, X Xiong, H Chen, J Xu Fibrochondrogenic differentiation potential of tendon-derived stem/progenitor cells from human patellar tendon J Orthop Translat, 2020;22(0):101-108. 2020 [PMID: 32440505] (Cell Culture, Human) Cell Culture Human
DA Mázala, JS Novak, MW Hogarth, M Nearing, P Adusumalli, CB Tully, NF Habib, H Gordish-Dr, YW Chen, JK Jaiswal, TA Partridge TGF-&amp;beta-driven muscle degeneration and failed regeneration underlie disease onset in a DMD mouse model JCI Insight, 2020;5(6):. 2020 [PMID: 32213706] (Cell Culture, Mouse) Cell Culture Mouse
HJ Zhao, HM Chang, C Klausen, H Zhu, Y Li, PCK Leung Bone morphogenetic protein 2 induces the activation of WNT/beta-catenin signaling and human trophoblast invasion through up-regulating BAMBI Cell. Signal., 2019;0(0):109489. 2019 [PMID: 31786181] (Cell Culture, Human) Cell Culture Human
D Seo, SM Jung, JS Park, J Lee, J Ha, M Kim, SH Park The deubiquitinating enzyme PSMD14 facilitates tumor growth and chemoresistance through stabilizing the ALK2 receptor in the initiation of BMP6 signaling pathway EBioMedicine, 2019;49(0):55-71. 2019 [PMID: 31685442] (Cell Culture, Human) Cell Culture Human
M Omi, V Kaartinen, Y Mishina Activin A receptor type 1-mediated BMP signaling regulates RANKL-induced osteoclastogenesis via canonical SMAD signaling pathway J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31619522] (Bioassay, Mouse) Bioassay Mouse
LR Dutton, CL O' Neill, RJ Medina, DP Brazil No evidence of Gremlin1-mediated activation of VEGFR2 signalling in endothelial cells J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31604823] (Cell Culture, Human) Cell Culture Human
J Ren, M Smid, J Iaria, DCF Salvatori, H van Dam, HJ Zhu, JWM Martens, P Ten Dijke Cancer-associated fibroblast-derived Gremlin 1 promotes breast cancer progression Breast Cancer Res., 2019;21(1):109. 2019 [PMID: 31533776] (Bioassay, Human) Bioassay Human
Li, Y Jiang, H Lin, H Shen, J Sohn, PG Alexander, RS Tuan Muscle injury promotes heterotopic ossification by stimulating local bone morphogenetic protein-7 production J Orthop Translat, 2019;18(0):142-153. 2019 [PMID: 31508317] (In Vivo, Mouse) In Vivo Mouse
K Krüger, S Schmid, F Paulsen, A Ignatius, P Klinger, T Hotfiel, B Swoboda, K Gelse Trefoil Factor 3 (TFF3) Is Involved in Cell Migration for Skeletal Repair Int J Mol Sci, 2019;20(17):. 2019 [PMID: 31480518] (Bioassay, Human) Bioassay Human
S Moghadasi, A Koontz, G Tseropoulo, L Kerosuo, P Mehrotra, VK Bajpai, SR Selvam, P Lei, ME Bronner, ST Andreadis Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo Sci Rep, 2019;9(1):9750. 2019 [PMID: 31278326] (Bioassay, Human) Bioassay Human
Show All 150 Publications.

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FAQs for BMP-2 (355-BM). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

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Bioinformatics

Gene Symbol BMP2
Uniprot