Collagen I: Novus Biologicals

NB600-408

Collagen I Antibody - BSA Free

(16 Reviews)

(322 Publications)

0.1 mg

NB600-408

Rat colon tissue stained with Collagen I antibody (red) and Hoechst (blue). IHC-P image submitted by a verified customer review.

Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.

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Primary human cardiac fibroblast cells were stained with anti-Collagen I antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf serum. ICC/IF image submitted by a verified customer review.

Cell lysate from human trabecular meshwork. Antibody at 1:1.000. WB image submitted by a verified customer review.

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Staining of human HaCaT-ras A5-RT3 (SCC) frozen tumor sections. Image provided by Dr. Wa'el Al Rawashdeh of RWTH Aachen University.

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Analysis in porcine burn wound lysate using anti-Collagen I alpha 1 antibody. WB image submitted by a verified customer review.

Detection of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2-2 ug/10 mL in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% non-fat milk. Detection occurred by incubation with a HRP conjugated secondary antibody at 1 ug/10 mL. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).

Balb/3T3 mouse embryonic fibroblast cell line stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Mouse pancreas tissue stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Human lung tissue section stained with Collagen I antibody at 1:400.

FFPE right lobe of the liver tissue section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Analysis of FFPE human lung. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Analysis of HRP conjugate of NB600-408. FFPE Human Skin at pH 6. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix.

Analysis of HRP conjugate of NB600-408. FFPE Human Skin tissue at pH9. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

Imaging of rat calvarial defect bone. IHC-P image submitted by a verified customer review.

<b>Biological Strategies Validation. </b>Collagen-I-Antibody-Immunohistochemistry-NB600-408-img0065.jpg

Analysis of Collagen I Antibody on alcohol-fixed rat lung tissue. Primary antibody dilution 1:200. Image from verified customer review.

Immunohistochemistry of Collagen I antibody. Tissue: human lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Immunohistochemistry of Rabbit Anti-collagen type I antibody. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Immunohistochemistry-Paraffin: Rabbit Polyclonal Collagen I Antibody [NB600-408] - Collagen I was stained in human FFPE tissue. Primary antibody dilution: 1/1000 in blocking buffer. HIER antigen retrieval at pH 9 for 20min. AF750 conjugated version of the antibody was used (Catalog # NB600-408AF750). Image from a verified customer review.

<b>Biological Strategies Validation. </b>Effects of fasudil treatment on tumor stroma in KPC mice.A) Pancreatic tumor tissues from vehicle, gemcitabine, or combination of gemcitabine plus fasudil treated KPC mice were harvested and stained for various stromal markers. Representative images are shown of H&E staining, alpha -SMA, Desmin, CD31, Collagen I, and Movat's pentachrome staining.

Western blot analysis. Comparison of collagen 1 (A and B) and collagen 2 (D and E) among the anterior horn, the body and the posterior horn: (A and D) young model; (B and E) adult model. Comparison of collagen 1 (C) and collagen 2 (F) content between the young and the adult specimens. Values with different superscripts differ for P < 0.01 (A,B). (G) Representative Western blot image for collagen 1, collagen 2 and GAPDH. N/group = 8.

EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(A) Cartoon of human EPS15 domain-organization and the amino-acid sequence. Indicated by color code are the SPOP/SPOPL binding site (red) and the lysine residue (yellow), which is ubiquitinated in a CRL3SPOPL–dependent manner in vivo. In addition, the amino-terminal Ca2+-binding EF-hand motifs (EH), the coiled-coil domain involved in dimerization and the two carboxy-terminal ubiquitin-interacting motifs (UIMs) involved in ubiquitin-binding are indicated. (B) EPS15 ubiquitin-profiling. Peptides containing EPS15 modification sites were quantified with LC-MS/MS after enrichment of the K-epsilon -GG motif from whole cell digests of HeLa cells treated with siSPOPL or siControl. Normalized precursor mass intensity profiles for EPS15 sites corresponding to K793, K801 and K693 are shown (raw data in Figure 4—figure supplement 1B). Quantification of the beta -Actin K113 and the polyubiquitin K11 linkage peptide control for comparable enrichment. Data are mean ± SD, N = 3. **p≤0.01. (C) Purified SPOPL was incubated as indicated with GST-tagged wild-type EPS15 or GST-EPS15 mutants, where the predicted SPOPL binding motifs have been mutated individually (GST-EPS15S605-S607A and EPS15S744-S746A, respectively), pulled down with glutathione sepharose (IP [GST]) and bound proteins were analyzed by Coomassie blue staining (upper panel) and immunoblotting (lower panels). Note that SPOPL readily binds to GST-EPS15 and GST-EPS15S605-S607A, but this interaction is strongly reduced with the GST-EPS15S744-S746A mutant. (D) HeLa cells stably expressing GFP-tagged wild-type EPS15, the EPS15S744-S746A or the EPS15K793R mutants from a doxycycline-inducible promoter were transfected as indicated (+) with control siRNA or siRNA depleting SPOPL. The levels of EPS15-GFP, EGFR and for control tubulin (TUB) were analyzed by immunoblotting with specific antibodies. Experiments were quantified in Fiji and the EPS15 levels plotted as fold-increase compar

Local release of anti-IL-6 from IMB suppresses LN fibrosis. The skin allografts were harvested at 7 days post-transplantation. (A-i) Lymphatic vessel expansion (Lyve-1+) & HEV elongation (MECA79+) were similar between all groups. (A-ii,iii) Dense staining of collagen I & PDPN was seen in DLNs harvested from control & GelMA group compared to those from GelMA/anti-IL-6 group. (representative images from 4 different mice per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31024011), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Phf2 protects the liver from high fat & high sucrose diet-induced fibrogenesis during obesity. GFP or Phf2 were overexpressed through AAV strategy specifically in the liver of 7-week-old male C57Bl/6 J mice. Mice were then fed with either a chow diet (NCD) or with a high fat & high sucrose diet (HFHSD) for 16 weeks. Mice were studied in the fed state. a Representative western blot analysis showing the contribution of Phf2 overexpression to the regulation of the PI3K/Akt signaling & pro-fibrogenic pathways in liver extracts (n = 15 per group). b (Left) Oral glucose tolerance test & insulin levels during the OGTT test. (right) Insulin tolerance test (n = 15 per group). c Liver sections stained with hematoxylin & eosin (H&E), trichrome masson & sirius red are shown. Scale bars = 100 μm (n = 10 per group). d Percentage of fibrotic area & percentage of apoptotic hepatocytes (n = 15 per group). e Relative oxidized GSSG content & measurement of Gpx activity (n = 10 per group). f Levels of carbonylated proteins (n = 10 mice per group). All error bars represent mean ± SEM. Statistical analyses were made using unpaired t-test (b) or Anova, followed by Bonferonni’s test (d, e). *P < 0.01 HFHSD/GFP compared to HFHSD/Phf2, $P < 0.01 HFHSD/GFP compared to NCD/GFP, £P < 0.01 HFHSD/Phf2 compared to HFHSD/GFP Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04361-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen I Antibody [NB600-408] - alpha -SMA, LOX, & COL1A1 immunostaining in human thyroid cancers. Representative thyroid tumors serial sections from two different patients stained by IHC for alpha -SMA, COL1A1, & LOX protein expression & localization. In the top panel, the tumor edge/invasive front is specifically shown (scale bar 500 µm), while lower panel has higher magnification (scale bar 200 µm). * clusters of tumor invading cells detaching from the principal tumor mass (T). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Increased co-localization of collagen type I with ER stress marker, KDEL in post-mortem human glaucomatous TM tissues. Post-mortem human TM tissues from age-matched normal (n = 3) & glaucoma (n = 3) were stained with Col I (green) & KDEL (red) & co-localization was examined in anterior segment tissues. Representative images are shown at lower (A) scale bar is 100 microns) & higher (B) scale bar is 25 microns) magnification. Both Col I & KDEL was increased in human glaucomatous TM tissues. In addition, increased co-localization of Col I with KDEL was observed in human glaucomatous TM tissues. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - Expression of osteoblastic differentiation markers in Human Gingival Mesenchymal Stem Cells. Adherent HGMSCs were cultured for 21 days in control medium or in differentiation medium (Diff) supplemented or not with 1 μM resveratrol (RV). At the end, cell homogenates were processed for western blotting analysis of the expression of the osteoblastic transcription factor RUNX-2 & of the osteogenic differentiation markers COL1A1, OPN & OCN. Densitometry of the specific bands (average ± S.D.) of three independent experiments is shown in the histograms Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31426798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Dex induced ECM synthesis is associated with ER stress in TM cells. Primary human TM cells (n = 3 strains) were treated with Veh or Dex (100 nM) for 3 days & stained for KDEL & fibronectin (A) or Col I (B). A prominent co-localization of fibronectin or Col I with KDEL was observed in Dex-treated TM cells indicating induction of ER stress & excessive intracellular ECM overload in primary TM cells. (C) GTM3 cells were treated with Veh or Dex for 3 days & total lysates were subjected to immunoprecipitation using KDEL antibody & immunoblotted for ECM proteins, GRP78 & GRP94. IgG antibody was used as a negative control. Total cellular lysates were used as input. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Dex increases Col I in the TM: Dex increases Col I & alpha -smooth muscle actin in the TM of Dex-treated mice (n = 3) compared with Veh-treated mice (n = 3). White box indicates TM. CB = ciliary body, I = Iris, C = cornea. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - Spautin-1 prevents the expression of osteoblastic differentiation markers in Human Gingival Mesenchymal Stem cells. HGMSCs were cultured for 21 days in control medium or in differentiation medium supplemented with 1 μM resveratrol (Diff + RV) in the absence or in the presence of spautin-1 (Sp1). At the end, cell homogenates were processed for western blotting analysis of the expression of the osteoblastic transcription factor RUNX-2 & of the osteogenic differentiation markers COL1A1, OPN & OCN. Densitometry of the specific bands (average ± S.D.) of three independent experiments is shown in the histograms Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31426798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Effects of fasudil treatment on tumor stroma in KPC mice.A) Pancreatic tumor tissues from vehicle, gemcitabine, or combination of gemcitabine plus fasudil treated KPC mice were harvested & stained for various stromal markers. Representative images are shown of H&E staining, alpha -SMA, Desmin, CD31, Collagen I, & Movat's pentachrome staining. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28841710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Immunofluorescence staining of osteoblastic differentiation markers in Human Gingival Mesenchymal Stem Cells. HGMSCs were plated on sterile coverslips, let adhere & cultured for 21 days in control medium or in differentiation medium (Diff) supplemented or not with 1 μM resveratrol (RV). At the end, the coverslips were fixed & processed for immunofluorescence staining of the osteogenic differentiation markers. Fluorescence staining was quantified with the ImageJ software Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31426798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - Collagen secretion defects in fibroblasts from patient 1a. (a) Control or patient fibroblasts incubated for 30 min to promote procollagen secretion were immunolabeled to detect COL1A1 (red) & in green, either Sec31A (to mark COPII‐coated ER exit sites) or GM130 (to mark the Golgi apparatus). (b) Extracellular collagen matrix was labeled to detect COL1A1. (c) Quantification of the mean Corrected Total Cell Fluorescence (CTCF) for COL1A1. Points show mean intensity from three independent experiments. Bars show standard deviation. (d) Immunoblot for COL1A1 in media (M) or lysates (L) of control or patient fibroblasts incubated in the presence of absence of ascorbate. (e) Unbiased proteomics was used to measure extracellular matrix collagens secreted from control or patient fibroblasts. Results from three independent experiments are shown. A ratio <1 indicates reduced collagen secretion from patient cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31568717), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - The effects of PA on TGF-beta 1-reduced PPAR gamma expression in HSC-T6 cells. (A,B) The effect of PA-induced PPAR gamma expression in rat hepatic stellate cells (HSCs). Cells were treated with PA (2 μM) for 0, 6, 12, & 24 h or PA (0.5, 1, & 2 μM) for 24 h, & PPAR gamma expression was analyzed by Western blotting. The effect of PA on TGF-beta 1-reduced PPAR gamma expression in rat HSCs; (C) cells were pretreated with various concentrations of PA (0.5, 1, & 2 μM) for 1 h, & then stimulated with TGF-beta 1 (5 ng/mL) for 24 h. Total protein extracted from cells was subjected to Western blotting to determine PPAR gamma expression; (D,E) cells were treated with TGF-beta 1 (5 ng/mL) for 24 h in the presence of PA and/or GW9662 (10 μM). Total protein extracted from cells was subjected to Western blotting to determine the expression of PPAR gamma & alpha -SMA & collagen I alpha 1. Protein bands were imaged using densitometry & analyzed using ImageJ software. The relative expression levels of target proteins were normalized using beta -actin as an internal control. The results are presented as the means ± SD of three independent experiments. # Significantly different from the control (p < 0.01). * Significantly different from the TGF-beta 1-treated group (p < 0.01). + Significantly different from the PA-treated group (p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - The effects of PA on TGF-beta 1-induced alpha -SMA & collagen I alpha 1 expression via suppression of the Akt & MAPK signal pathways in HSC-T6 cells. (A,B) The inhibitory effects of PA on TGF-beta 1-induced alpha -SMA & collagen I alpha 1 expression via suppression of Akt & MAPK in rat hepatic stellate cells (HSCs); (A) cells were treated with TGF-beta 1 (5 ng/mL) for 24 h in the presence of LY294002 (LY; 10 μM), PD98059 (PD; 20 μM), SB203580 (SB; 20 μM), or SP600125 (SP; 20 μM); (B) cells were treated with TGF-beta 1 (5 ng/mL) for 24 h in the presence of PA & LY294002 (LY; 10 μM), PD98059 (PD; 20 μM), SB203580 (SB; 20 μM), or SP600125 (SP; 20 μM). Total protein extracted from cells was subjected to Western blotting to determine alpha -SMA & collagen I alpha 1 expression. Protein bands were imaged using densitometry & analyzed using ImageJ software. The relative expression levels of target proteins were normalized using beta -actin as an internal control. The results are presented as the means ± SD of three independent experiments. # Significantly different from the control (p < 0.01). * Significantly different from the TGF-beta 1-treated group (p < 0.01). + Significantly different from the PA-treated group (p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Cells expressing IL-1⍺ & IL-1R are observed in the fibrotic liver environment. (a) Cytokines in tissue lysates from mice at 9 wpi were measured by ELISA in liver. Data are presented as fold change relative to the mean of uninfected levels. N = 11–12 mice per group, pooled from three independent experiments. (b) IL-1 alpha levels in the sera at 9 wpi measured by ELISA. N = 9–14 mice per group, pooled from four independent experiments. (c) Immunofluorescence labeling of nuclei (DAPI white), IL-1⍺ (green), CD45 (red), & collagen1⍺1 (blue) in the liver of UI or 9 wpi WT mice. Number of cells staining positive for CD45 and/or IL-1⍺, average 2–3 fields of view where immune infiltrate was present from 3 mice per condition are quantified on the right. Error bars are standard deviation. (d) Immunofluorescent labeling of nuclei (DAPI white), ⍺-smooth muscle actin (green), IL-1R (red), & collagen1⍺ (blue) in the liver of uninfected or 9 wpi WT. Inset, arrow head represents ⍺-smooth muscle actin/IL-1R co-staining cells (arrow heads). Number of cells staining positive for IL-1R and/or ⍺-SMA, average of 4–8 fields of view from 3 mice are quantified on the right. Error bars are standard deviation. (c,d) represent maximum intensity projections of 9–13 μm thick z-stacks. Scale bar represents 50 μm. Error bars are standard error of the mean except where noted otherwise. *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32973293), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Characterization of decellularized heart matrix for DNA & extracellular matrix content with DNA quantification, histological staining, & multiphoton imaging. (A) DNA content of the right ventricle (RV, triangles), septum (ST, circles), & left ventricle (LV, squares) for cadaveric hearts compared to decellularized Langendorff (n = 4) or 4-Flow cannulated hearts (n = 4). (B) Masson’s trichrome staining of representative Langendorff (n = 4) & 4-Flow (n = 4) hearts for preserved collagen (blue) & clearance of nuclear remnants (absence of positively stained purple nuclei). (C) Multiphoton microscopy visualization of collagen (pseudocolored - cyan) & elastin (pseudocolored - yellow) fibrillar strcutures & organization in 3D (n = 4). (D) Immunostaining of histology sections important extracellular matrix proteins: collagen I, collagen IV, & laminin (n = 4). (E) The list of preserved extracellular matrix proteins in decellularized 4-Flow hearts as detected by mass spectrometry (n = 4). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-25883-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - PARP1 regulates VSMCs osteogenic transition, not Na/P co-transporter. a, b Quantitative RT-PCR (a) & western blot (b) analysis of PIT1 & PIT2 expression in VSMCs, which were infected with Scrambled (Scr shRNA) or PARP1 shRNA, & treated with high Pi. c–e Rat VSMCs were infected with adenovirus carrying Scr shRNA, PARP1 shRNA, Null or PARP1 gene for 48 h & then incubated with osteogenic media for 14 days. PARP1 inhibitors 3AB (10 mM) & PJ34 (10 μM) were added every day. Western blot analysis & related quantification of osteogenic-related marker genes (OPN, ColIA1, & OC) & smooth muscle marker genes (SMA, SM22 & SMMHC) expression in VSMCs with PARP1 knockdown (c), PARP1 inhibitors 3AB & PJ34 (d), & PARP1 overexpression (e). (n = 5 for each group). Statistical significance was assessed using one-way ANOVA for multiple comparison & two-tailed t-tests for two groups & is presented as follows: NS: no significance, **P < 0.01 & ##P < 0.01. All values are means ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30867423), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - CM derived from OS cells stimulates the BM‐MSCs trans‐differentiation into CAF‐like cells. (A) FACS analysis of NG‐2 & CD31 expression in BM‐MSCs treated for 48 h with CM OS cells. (B) BM‐MSCs were stimulated for 48 h with CM from OS cells & alpha ‐SMA & Col I‐ alpha 1 expression was assessed by immunoblot analysis. Results are representative of four biological replicates. (C) Collagen contraction assay of BM‐MSCs treated for 24 h with St Med or HOS CM. Data are expressed as percentages of the relative area of collagen disc following contraction in comparison with an empty well (mean ± SD, n = 3 biological replicates performed in duplicate). * P < 0.05 vs. St Med. (D) Migration assay of HOS cells stimulated for 48 h with CM derived from BM‐MSCs previously activated or not activated by tumour cells (CM BM‐MSCs St/HOS). Cells were allowed to migrate toward complete medium (FBS 10%). Untreated cells (St Med) were used as control. Results are the mean ± SEM of three biological replicates. *** P < 0.001 vs. St Med. (E) Invasion & transmigration (F) assays of HOS cells treated as in (D) (mean ± SD, n = 3 independent experiments). ** P < 0.005 vs. St Med; *** P < 0.001 vs. St Med. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29517849), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Human PRP infusion improves regeneration of the damaged endometrium & down-regulates expression of fibrosis-related factors. (A) Design of Experiment 1. In AS mice, PRP was injected only into the right horn at 7 days after inducing injury to the bilateral uterine horns. At 14 days, all mice were sacrificed & the uterine horns were prepared for analysis. (B) Hematoxylin & eosin staining of the endometrial tissues to evaluate morphologic structures in the PRP-treated horn of AS mice. (C) Masson’s trichrome staining to evaluate collagen deposition (blue) in the PRP-treated horns of AS mice. (D) Immunofluorescence staining of collagen type 1A (COL1A1) in the PRP-treated horn of AS mice. Green & red indicate COL1A1 & nuclei, respectively. (E) RT-qPCR & (F) real-time RT-qPCR analyses of expression of fibrosis-related factors in the PRP-treated horn of AS mice. Sham, n = 4; AS or PRP-treated AS, n = 5. *p < 0.01; Scale bar: 100 & 200 μm. AS, Asherman’s syndrome; PCR, polymerase chain reaction; PRP, platelet-rich plasma; RT-qPCR, reverse transcription-quantitative PCR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32116803), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Fibroblast-derived CDM induces sustained growth inhibition of cancer cells.(a) Collagen I & fibronectin staining of CDM generated by NFs (TIFFs). Scale bar, 20 μm. (b) Proliferation of MDA-MB-231 & HeLa cells plated on TIFF CDM or on plastic in full medium for the indicated times. n(HeLa)=10, n(MDA-MB-231)=8. (c) Proliferation of MDA-MB-231 & HeLa cells after detachment from TIFF matrices (6 days on matrix before detachment) & replating on plastic in full medium for the indicated times n(HeLa)=7, n(MDA-MB-231)=10. (d) Representative images of MDA-MB-231 cell morphology on CDM & plastic. Shown are maximum intensity projections of confocal images. Scale bar, 10 μm. (e) Schematic representation of the experimental set-up. Red arrows indicate the time points of sample collection for Illumina gene expression analysis. (f) Common gene expression changes in MDA-MB-231 & HeLa cells on CDM & 5 days after CDM detachment (CDM to plastic (pl.)). The numbers of commonly regulated genes (up- or downregulated) in both cell lines are indicated in the table. Upregulated genes are marked with red & downregulated genes with blue. All data are mean±s.e.m. Unpaired t-test was used for statistical analyses. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27488962), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Spautin-1 prevents the autophagy-associated osteoblastic differentiation of Human Gingival Mesenchymal Stem cells. a HGMSCs were plated on sterile coverslips, let adhere & cultured for 21 days in control medium or in differentiation medium supplemented with 1 μM resveratrol (Diff + RV) in the absence or the presence of spautin-1 (Sp1). The coverslips were then fixed & processed for immunofluorescence staining of the autophagy marker LC3 & of the osteoblastic differentiation marker OCN. As an index of autophagy in the cells, LC3 puncta were quantified (as per the guidelines 36). b HGMSCs were plated & treated as in a & the coverslips processed for immunofluorescence staining of the osteoblastic differentiation markers OCN & COL1A1. Integrated fluorescence intensity quantified with the ImageJ software is shown in the histograms. c HGMSCs cells were plated on plastic & treated as in a & at the end the cultures were processed for Alizarin Red S staining of extracellular calcium deposits. The stained area was quantified using the ImageJ software Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31426798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Collagen secretion defects in fibroblasts from patient 1a. (a) Control or patient fibroblasts incubated for 30 min to promote procollagen secretion were immunolabeled to detect COL1A1 (red) & in green, either Sec31A (to mark COPII‐coated ER exit sites) or GM130 (to mark the Golgi apparatus). (b) Extracellular collagen matrix was labeled to detect COL1A1. (c) Quantification of the mean Corrected Total Cell Fluorescence (CTCF) for COL1A1. Points show mean intensity from three independent experiments. Bars show standard deviation. (d) Immunoblot for COL1A1 in media (M) or lysates (L) of control or patient fibroblasts incubated in the presence of absence of ascorbate. (e) Unbiased proteomics was used to measure extracellular matrix collagens secreted from control or patient fibroblasts. Results from three independent experiments are shown. A ratio <1 indicates reduced collagen secretion from patient cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31568717), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - The effects of PA on TGF-beta 1-induced alpha - SMA & collagen I alpha 1 expression in HSC-T6 cells. (A,B) The inhibitory effect of PA on TGF-beta 1-induced alpha -SMA & collagen type I mRNA & protein expression in rat hepatic stellate cells. Cells were pretreated with 0.5, 1, & 2 μM PA for 1 h, & then stimulated with TGF-beta 1 (5 ng/mL) for 24 h. Total RNA extracted from cells was analyzed by the real-time polymerase chain reaction to determine alpha -SMA & ColIa1 mRNA expression; (C) the total protein extracted from cells was subjected to Western blotting to determine alpha -SMA & collagen I alpha 1 expression. Protein bands were imaged using densitometry & analyzed using ImageJ software. The relative expression levels of target proteins were normalized using beta -actin as an internal control. The results are expressed as the means ± SD of three independent experiments. # Significantly different from the control (p < 0.01). * Significantly different from the TGF-beta 1-treated group (p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Frozen: Collagen I Antibody [NB600-408] - Cryopreserved murine brain tissue stained with Collagen I Antibody [Alexa Fluor® 647] (Catalog # NB600-408AF647) (red) and Hoechst (blue). Image from a verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Bv, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Rb

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-408AF350
Alexa Fluor 405	NB600-408AF405
Alexa Fluor 488	NB600-408AF488
Alexa Fluor 532	NB600-408AF532
Alexa Fluor 594	NB600-408AF594
Alexa Fluor 647	NB600-408AF647
Alexa Fluor 700	NB600-408AF700
Alexa Fluor 750	NB600-408AF750
Alexa Fluor Plus 405	NB600-408AFP405
Alexa Fluor Plus 488	NB600-408AFP488
Alexa Fluor Plus 555	NB600-408AFP555
Alexa Fluor Plus 594	NB600-408AFP594
Alexa Fluor Plus 647	NB600-408AFP647
Alexa Fluor Plus 680	NB600-408AFP680
Allophycocyanin	NB600-408APC
Biotin	NB600-408B
CoraFluor 1	NB600-408CL1
DyLight 350	NB600-408UV
DyLight 405	NB600-408V
DyLight 488	NB600-408G
DyLight 550	NB600-408R
DyLight 594	NB600-408DL594
DyLight 650	NB600-408C
DyLight 680	NB600-408FR
DyLight 755	NB600-408IR
FITC	NB600-408F
HRP	NB600-408H
Janelia Fluor 525	NB600-408JF525
Janelia Fluor 549	NB600-408JF549
Janelia Fluor 646	NB600-408JF646
Janelia Fluor 669	NB600-408JF669
PE	NB600-408PE
PerCP	NB600-408PCP
mFluor Violet 450 SE	NB600-408MFV450
mFluor Violet 500 SE	NB600-408MFV500
mFluor Violet 610 SE	NB600-408MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-408-0.025mg

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NBP1-30054

Collagen I alpha 1 Antibody - Azide and BSA Free

(2 Reviews)

(82 Publications)

0.1 ml

NBP1-30054

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.

Simple Western: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - TNC deficiency reduced kidney fibrosis in animal models.Deletion of TNC (TNC−/−) significantly attenuated the induction of collagen I assessed by IF following UUO by approximate 30% at day 7 & 10 compared with their wild-type littermates (A, n = 4 for each time point, two-way ANOVA p < 0.05). Consistently, the expression of fibrosis markers, such as collagen I alpha , fibronectin & plasminogen activator inhibitor-1 (PAI-1), were significantly lower in TNC−/− mice at UUO day 7 & 10 (B, n = 4 for each time point, two-way ANOVA, p < 0.05). Western blot showed that the proteins of collagen I alpha & alpha -SMA were also significantly reduced in TNC−/− mice at UUO day 7 (C, n = 7, p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36522320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Pathological staining analysis of 28-day animal experiment induced by BAPN. (A), representative images from elastic van Gieson (EVG) staining, along with statistical analysis of elastin degradation. (B), representative images from hematoxylin and eosin (HE) staining are presented, accompanied by statistical analysis of aortic wall thickness. The scale bar for both panels (A) and (B) is 200 μm. (C), immunofluorescence staining of Col1a1, accompanied by quantitative analysis of the Col1a1 positive area in the vascular wall, scale bar 50 μm Image collected and cropped by CiteAb from the following open publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-024-00837-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Am, Av, Ma, Sh, Bv, Ca, Ch, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Vo, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Am, Av, Ma, Sh, Bv, Ca, Ch, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Vo, Xp

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP1-30054-0.02ml

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NB600-450

Collagen I alpha 1 Antibody (COL-1)

(5 Reviews)

(68 Publications)

0.1 ml

NB600-450

<b>Biological Strategies Validation. </b>Collagen-I-alpha-1-Antibody-COL-1-Immunocytochemistry-Immunofluorescence-NB600-450-img0014.jpg

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary antibody. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and used at a dilution of 1:500.

Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-NB600-450-img0013.jpg

Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Mouse healthy liver. Anibody at 1:200. Enzymatic/proteolytic antigen retrieval. No permeabilization. IHC-P image submitted by a verified customer review.

(a) Histological section of keloid stained with picrosirius seen in ordinary light (20x). (b) Histological section of keloid stained with picrosirius seen in polarized light (20x). (c) Immunohistochemistry for type I collagen in keloid (20x). (d) Histological section of keloid stained with picrosirius seen in ordinary light (20x). (e) Histological section of keloid stained with picrosirius seen in polarized light (20x). (f) Immunohistochemistry for type III collagen in keloid (20x).

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - FSP‐1–positive cells only partially responsible for collagen I & III production in irradiated bladders. Bladder strips from irradiated mice were costained for collagen I or for collagen III mRNA (pink) & for fibroblasts (FSP‐1, brown). U: urothelium; LP: lamina propria. Black arrow: FSP‐1 positive cells. Gray arrow: collagen only positive cells. Scale bar = 50 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for collagen I or for collagen III and imaged using fluorescent microscopy. (a) Representative images (100 x 100 um) of each mouse strain and treatment group are provided for collagen I and collagen III immunofluorescence. (b–c) Percentage of tissue‐positive for collagen I or collagen III staining. Collagen I and III density is significantly elevated in C57BL/6 mice, but not in the C3H or BALB/c strains. Results are mean +/- SD of n = 3–6 mice. Dashed line: ANOVA; Full line: multiple t‐test. **p < .01, *** p < .001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, Rb

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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DY6220-05

Human Pro-Collagen I alpha 1 DuoSet ELISA, 5 Plate

(63 Publications)

1 Kit (for 5 Plates)

DY6220-05

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF6220

Collagen I alpha 1 Antibody [Unconjugated]

(27 Publications)

100 μg

AF6220

HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human kidney tissue, human skin tissue, and human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human kidney tissue, human skin tissue, and human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF016). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of IMR‑90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Pro-Collagen I alpha 1 was detected in immersion fixed IMR-90 human lung fibroblast cell line using Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB6220) under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Human N-Pro Collagen I was immunoprecipitated from 500 μg of IMR‑90 human lung fibroblast cell line lysates with 5 μg of Sheep Anti-Human N-Pro Collagen I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220). The N-Pro Collagen I-antibody complexes were absorbed using Protein A or Protein G. Immunoprecipitated human N-Pro Collagen I was detected by Western blot using 1 μg/mL of Mouse Anti-Human N-Pro Collagen I Monoclonal Antibody (<a class=

MAB6220) under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of IMR‑90 human lung fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Collagen I alpha 1 at approximately 232 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Pro Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6220U-100UG
Alexa Fluor 405	AF6220V-100UG
Alexa Fluor 488	AF6220G-100UG
Alexa Fluor 532	AF6220X-100UG
Alexa Fluor 594	AF6220T-100UG
Alexa Fluor 647	AF6220R-100UG
Alexa Fluor 700	AF6220N-100UG
Alexa Fluor 750	AF6220S-100UG
Alexa Fluor Plus 405	AF6220AFP405-100UG
Alexa Fluor Plus 488	AF6220AFP488-100UG
Alexa Fluor Plus 555	AF6220AFP555-100UG
Alexa Fluor Plus 594	AF6220AFP594-100UG
Alexa Fluor Plus 647	AF6220AFP647-100UG
Alexa Fluor Plus 680	AF6220AFP680-100UG
Allophycocyanin	AF6220A
Biotin	AF6220B
CoraFluor 1	AF6220CL1
DyLight 350	AF6220D
DyLight 405	AF6220E
DyLight 488	AF6220K
DyLight 550	AF6220L
DyLight 594	AF6220M
DyLight 650	AF6220W
DyLight 680	AF6220Y
DyLight 755	AF6220Z
FITC	AF6220F
HRP	AF6220H
Janelia Fluor 525	AF6220JF525
Janelia Fluor 549	AF6220I
Janelia Fluor 646	AF6220J
Janelia Fluor 669	AF6220JF669
PE	AF6220P
PerCP	AF6220C
mFluor Violet 450 SE	AF6220MFV450
mFluor Violet 500 SE	AF6220MFV500
mFluor Violet 610 SE	AF6220MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6220-SP

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NBP1-77457

Collagen I alpha 1 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-77457

Reactivity:

Hu, Mu, Rt, Bv, Ca, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, Sh

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77457AF350
Alexa Fluor 405	NBP1-77457AF405
Alexa Fluor 488	NBP1-77457AF488
Alexa Fluor 532	NBP1-77457AF532
Alexa Fluor 594	NBP1-77457AF594
Alexa Fluor 647	NBP1-77457AF647
Alexa Fluor 700	NBP1-77457AF700
Alexa Fluor 750	NBP1-77457AF750
Alexa Fluor Plus 405	NBP1-77457AFP405
Alexa Fluor Plus 488	NBP1-77457AFP488
Alexa Fluor Plus 555	NBP1-77457AFP555
Alexa Fluor Plus 594	NBP1-77457AFP594
Alexa Fluor Plus 647	NBP1-77457AFP647
Alexa Fluor Plus 680	NBP1-77457AFP680
Allophycocyanin	NBP1-77457APC
Biotin	NBP1-77457B
CoraFluor 1	NBP1-77457CL1
DyLight 350	NBP1-77457UV
DyLight 405	NBP1-77457V
DyLight 488	NBP1-77457G
DyLight 550	NBP1-77457R
DyLight 594	NBP1-77457DL594
DyLight 650	NBP1-77457C
DyLight 680	NBP1-77457FR
DyLight 755	NBP1-77457IR
FITC	NBP1-77457F
HRP	NBP1-77457H
Janelia Fluor 525	NBP1-77457JF525
Janelia Fluor 549	NBP1-77457JF549
Janelia Fluor 646	NBP1-77457JF646
Janelia Fluor 669	NBP1-77457JF669
PE	NBP1-77457PE
PerCP	NBP1-77457PCP
mFluor Violet 450 SE	NBP1-77457MFV450
mFluor Violet 500 SE	NBP1-77457MFV500
mFluor Violet 610 SE	NBP1-77457MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77457SS

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NBP1-77458

Collagen I alpha 1 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-77458

Reactivity:

Hu, Mu, Po, Bt, Bv, Ca, Eq, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Po, Bt, Bv, Ca, Eq, Pm, Sh

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77458AF350
Alexa Fluor 405	NBP1-77458AF405
Alexa Fluor 488	NBP1-77458AF488
Alexa Fluor 532	NBP1-77458AF532
Alexa Fluor 594	NBP1-77458AF594
Alexa Fluor 647	NBP1-77458AF647
Alexa Fluor 700	NBP1-77458AF700
Alexa Fluor 750	NBP1-77458AF750
Alexa Fluor Plus 405	NBP1-77458AFP405
Alexa Fluor Plus 488	NBP1-77458AFP488
Alexa Fluor Plus 555	NBP1-77458AFP555
Alexa Fluor Plus 594	NBP1-77458AFP594
Alexa Fluor Plus 647	NBP1-77458AFP647
Alexa Fluor Plus 680	NBP1-77458AFP680
Allophycocyanin	NBP1-77458APC
Biotin	NBP1-77458B
CoraFluor 1	NBP1-77458CL1
DyLight 350	NBP1-77458UV
DyLight 405	NBP1-77458V
DyLight 488	NBP1-77458G
DyLight 550	NBP1-77458R
DyLight 594	NBP1-77458DL594
DyLight 650	NBP1-77458C
DyLight 680	NBP1-77458FR
DyLight 755	NBP1-77458IR
FITC	NBP1-77458F
HRP	NBP1-77458H
Janelia Fluor 525	NBP1-77458JF525
Janelia Fluor 549	NBP1-77458JF549
Janelia Fluor 646	NBP1-77458JF646
Janelia Fluor 669	NBP1-77458JF669
PE	NBP1-77458PE
PerCP	NBP1-77458PCP
mFluor Violet 450 SE	NBP1-77458MFV450
mFluor Violet 500 SE	NBP1-77458MFV500
mFluor Violet 610 SE	NBP1-77458MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77458SS

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NBP1-97266

Collagen I Native Protein

(2 Publications)

0.5 mg

NBP1-97266

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

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NBP2-75839

Human Collagen I alpha 1 ELISA Kit (Colorimetric)

1 Kit

NBP2-75839

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.31 - 20 ng/mL

Sensitivity:

0.19 ng/mL

Sample Type:

Serum, plasma and other biological fluids

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6220-CL

Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein, CF

(7 Publications)

20 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-92858

Collagen I alpha 1 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP2-92858

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92858] - Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using Collagen I alpha 1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-92858-0.02ml

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NBP2-75840

Rat Collagen I alpha 1 ELISA Kit (Colorimetric)

1 Kit

NBP2-75840

ELISA: Rat Collagen I alpha 1 ELISA Kit (Colorimetric) [NBP2-75840] - Samples were spiked with high concentrations of Rat Collagen I alpha 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Collagen I alpha 1 ELISA Kit (Colorimetric) [NBP2-75840] - Standard Curve Reference

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Standard Curve:

0.31 - 20 ng/mL

Sensitivity:

0.19 ng/mL

Sample Type:

Serum, plasma and other biological fluids

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

COL1A1 Products By Gene Name

Collagen I Antibody - BSA Free

Collagen I alpha 1 Antibody - Azide and BSA Free

Collagen I alpha 1 Antibody (COL-1)

Human Pro-Collagen I alpha 1 DuoSet ELISA, 5 Plate

Collagen I alpha 1 Antibody [Unconjugated]

Collagen I alpha 1 Antibody - BSA Free

Collagen I alpha 1 Antibody - BSA Free

Collagen I Native Protein

Human Collagen I alpha 1 ELISA Kit (Colorimetric)

Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein, CF

Collagen I alpha 1 Antibody - BSA Free

Rat Collagen I alpha 1 ELISA Kit (Colorimetric)