Bestrophin 1 Antibody (E6-6)


Western Blot: Bestrophin 1 Antibody (E6-6) [NB300-164] - Detection of Bestrophin (68 kDa) from human RPE cell lysate.

Product Details

Reactivity Hu, Rt(-)Species Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Bestrophin 1 Antibody (E6-6) Summary

Synthetic peptide conjugated to KLH corresponding to the C-terminus of human Bestrophin 1 (KDHMDPYWALENRDEAHS) [UniProt# O76090]
Bestrophin (E6-6)
Predicted Species
Porcine (94%), Primate (100%)
IgG1 Kappa
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence Assay dependent
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
By Western blot, this antibody recognizes a band at ~68 kDa representing Bestrophin.** It is also useful for immunofluorescence. **Please see protocol for treatment of cell extract. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24345323)
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NB300-164 in the following applications:

Reactivity Notes

NB300-164 recognizes the human protein. The immunogen shares 88% homology to canine, 94% to porcine, and 100% to chimpanzee. This antibody does not detect the rat protein. Other species have not been tested.

Alternate Names for Bestrophin 1 Antibody (E6-6)

  • ARB
  • Best disease
  • BEST
  • bestrophin 1
  • bestrophin-1
  • BMD
  • TU15B
  • vitelliform macular dystrophy 2
  • Vitelliform macular dystrophy protein 2
  • VMD2RP50


Best macular dystrophy (BMD) or vitelliform macular dystrophy (VMD2), is an autosomal form of macular degeneration, characterized by a depressed light peak in the electrooculogram (EOG). It is inherited and has an early onset. Bestrophin is a 68 kDa basolateral plasma membrane protein encoded by the VMD2 gene. Bestrophin's function is still unknown, but data suggests that it is a chloride channel that plays a role in generating the altered EOG in Best disease patients. In addition, Bestrophin is a useful biochemical and histological marker of RPE (retinal pigment epithelial cells) cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bestrophin 1 Antibody (NB300-164)(20)

We have publications tested in 5 confirmed species: Human, Canine, Porcine, Porcine , Primate.

We have publications tested in 5 applications: ICC/IF, IHC-Fr, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB300-164 Applications Species
Parker J, Mitrousis N, Shoichet MS. Hydrogel for Simultaneous Tunable Growth Factor Delivery and Enhanced Viability of Encapsulated Cells in Vitro. Biomacromolecules. 2016 Feb 08 [PMID:26762290] (ICC/IF, Human) ICC/IF Human
Bertolotti E, Neri A, Camparini M et al. Stem cells as source for retinal pigment epithelium transplantation. Prog Retin Eye Res. 2014 Jun 13 [PMID:24933042] (ICC/IF, Human) ICC/IF Human
Sharon D, Al-Hamdani S, Engelsberg K et al. Ocular phenotype analysis of a family with biallelic mutations in the BEST1 gene. Am J Ophthalmol 2013 Dec 14 [PMID:24345323] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Maruotti J, Wahlin K, Gorrell D et al. A simple and scalable process for the differentiation of retinal pigment epithelium from human pluripotent stem cells. Stem Cells Transl Med 2013 May [PMID:23585288]
Blenkinsop TA, Salero E, Stern JH, Temple S. The culture and maintenance of functional retinal pigment epithelial monolayers from adult human eye Methods Mol Biol 2013 [PMID:23097100]
Cho MS, Kim SJ, Ku SY et al. Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses Stem Cell Res 2012 May 16 [PMID:22683799] (ICC/IF, Human) ICC/IF Human
Ukrohne TU, Westenskow PD, Kurihara T et al. Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells. Stem Cells Transl Med 2012 Feb [PMID:22532929] (ICC/IF, Human) ICC/IF Human
Guziewicz KE, Slavik J, Lindauer SJ et al. Molecular consequences of BEST1 gene mutations in canine multifocal retinopathy predict functional implications for human bestrophinopathies. Invest Ophthalmol Vis Sci. 2011 Apr 15. [PMID:21498618] (WB, Canine) WB Canine
Davidson AE, Millar ID, Burgess-Mullan R et al. Functional characterization of bestrophin-1 missense mutations associated with autosomal recessive bestrophinopathy. Invest Ophthalmol Vis Sci 2011 May [PMID:21330666]
Harness JV, Turovets NA, Seiler MJ et al. Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells. PLoS ONE;6(1). 2011 [PMID:21249129] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 20 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bestrophin 1 Antibody (NB300-164). (Showing 1 - 4 of 4 FAQs).

  1. According to the data sheet, for Immunohistochemistry, it references (PMID: 21249129). I see a 1:25 dilution in the journal article referenced under immunocytochemistry. Is this the same dilution recommended for IHC? If not do you have a recommended dilution? Antigen retrieval: Do you have any information on AR? Citrate buffer or higher pH (EDTA)? Temperature? Time? Incubation: Is 1 hour at room temperature or overnight at 4C recommended?
    • This antibody was validated in IHC in the reference PMID 11050159. Generally we recommend using antigen retrieval with citrate buffer and incubating for 1 hour at RT. Some antigens may be tricky and require overnight incubation. I imagine the specific protocol can be found in the reference, however.
  2. Can I get the recipe for RIPA buffer and the lysate preparation protocol for membrane bound proteins using NB300-164?
    • Here is both the recipe we use for RIPA and a good protocol for tissue lysate prep. RIPA buffer: 150 mM sodium chloride 1.0% NP-40 or Triton X-100 0.5% sodium deoxycholate 0.1% SDS (sodium dodecyl sulphate) 50 mM Tris, pH 8.0 RIPA buffer is also useful for whole cell extracts and membrane-bound proteins, and may be preferable to NP-40 or Triton X100-only buffers for extracting nuclear proteins. It will disrupt protein-protein interactions and may therefore be problematic for immunoprecipitations/pull down assays. Preparation of lysate from tissues Dissect the tissue of interest with clean tools, on ice preferably, and as quickly as possible to prevent degradation by proteases. Place the tissue in round-bottom microfuge tubes or Eppendorf tubes and immerse in liquid nitrogen to snap freeze. Store samples at -80C for later use or keep on ice for immediate homogenization. For a approximately 5 mg piece of tissue, add approximately 300 ul lysis buffer rapidly to the tube, homogenize with an electric homogenizer, rinse the blade twice with another 2x300 ul lysis buffer, then maintain constant agitation for 2 hours at 4C (e.g place on an orbital shaker in the fridge). Volumes of lysis buffer must be determined in relation to the amount of tissue present (protein extract should not be too diluted to avoid loss of protein and large volumes of samples to be loaded onto gels. The minimum concentration is 0.1 mg/ml, optimal concentration is 1-5 mg/ml). Centrifuge for 20 min at 12000 rpm at 4C in a microcentrifuge. Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice; discard the pellet.
  3. I want to know the concentration of Bestrophin 1 antibody [NB300-164].
    • For our product NB300-164, this antibody is provided as unpurified ascites. Therefore, the antibody concentration has not been determined.
  4. We are interested in your item, NB300-164 and would you please let us know the antibody concentration of it?
    • The total protein concentration of the ascites (Lot# B-3) is 11.7 mg/ml.

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Blogs on Bestrophin 1.

Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

Bestrophin 1: Implications in Progressive Vision Loss
The human Bestrophin family has four members, Best1, Best2, Best3 and Best4. These transmembrane proteins can function as chloride channels, and can also regulate calcium channels (1). The Bestrophins all have a conserved domain which begins at the N-...  Read full blog post.

"I can see clearly now": Targeting Bestrophin 1 to treat Bestrophinopathies
Encoded by the VMD2 gene on chromosome 11q13 Bestrophin 1 is the prototypic member of the RFP family of proteins which are more commonly called "bestrophins". The protein family was originally identified in Caenorhabditis elegans based on a conserved...  Read full blog post.

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Product PDFs


Gene Symbol BEST1

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