Bestrophin 1 Antibody (E6-6)


Western Blot: Bestrophin 1 Antibody (E6-6) [NB300-164] - Detection of Bestrophin (68 kDa) from human RPE cell lysate.
Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Bestrophin 1 (E6-6) Monoclonal more

Product Details

Reactivity Hu, Po, Ca, Rt(-), PmSpecies Glossary
Applications WB, IHC-Fr, IHC-P, IP

Order Details

Bestrophin 1 Antibody (E6-6) Summary

Synthetic peptide conjugated to KLH corresponding to the C-terminus of human Bestrophin 1 (KDHMDPYWALENRDEAHS) [Uniprot: O76090]
Bestrophin (E6-6)
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
IgG1 Kappa
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
In Western blot, this antibody recognizes a band at ~68 kDa representing Bestrophin. Please see protocol for treatment of cell extracts. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 24345323).
Reviewed Applications
Read 1 Review rated 5
NB300-164 in the following applications:

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NB300-164 in the following applications:

Reactivity Notes

Predicted to react with chimpanzee (100%). This antibody does not detect the rat protein. Other species have not been tested. Canine reactivity reported in scientific literature (PMID: 27519691). Porcine reactivity reported in scientific literature (PMID: 28687758).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1% Sodium Azide

Alternate Names for Bestrophin 1 Antibody (E6-6)

  • ARB
  • Best disease
  • BEST
  • bestrophin 1
  • bestrophin-1
  • BMD
  • TU15B
  • vitelliform macular dystrophy 2
  • Vitelliform macular dystrophy protein 2
  • VMD2RP50


Best macular dystrophy (BMD) or vitelliform macular dystrophy (VMD2), is an autosomal form of macular degeneration, characterized by a depressed light peak in the electrooculogram (EOG). It is inherited and has an early onset. Bestrophin is a 68 kDa basolateral plasma membrane protein encoded by the VMD2 gene. Bestrophin's function is still unknown, but data suggests that it is a chloride channel that plays a role in generating the altered EOG in Best disease patients. In addition, Bestrophin is a useful biochemical and histological marker of RPE (retinal pigment epithelial cells) cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bestrophin 1 Antibody (NB300-164)(29)

We have publications tested in 6 confirmed species: Human, Rat, Canine, Porcine, Porcine , Primate.

We have publications tested in 6 applications: ICC/IF, IHC-Fr, IHC-P, IP, WB, WB,ICC/IF.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB300-164 Applications Species
Zhang Y, Kittredge A, Ward N et al. ATP activates bestrophin ion channels through direct interaction Nat Commun Aug 7 2018 [PMID: 30087350] (ICC/IF, Human) ICC/IF Human
Li Y, Zhang Y, Xu Y et al. Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca(2+)-dependent Cl(-) currents in human RPE. Elife. 2017 Oct 24 [PMID: 29063836] (Human) Human
Zhang Y, Zhang D, Wei W et al. Effects of RPE-conditioned medium on the differentiation of hADSCs into RPE cells, and their proliferation and migration. Exp Ther Med. 2017 Oct 01 [PMID: 29042966] (ICC/IF, Human) ICC/IF Human
Klingeborn M, Dismuke WM, Skiba NP et al. Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers Sci Rep 2017 Jul 07 [PMID: 28687758] (ICC/IF, Porcine) ICC/IF Porcine
Shim SH, Kim G, Lee DR et al. Survival of Transplanted Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells in a Human Recipient for 22 Months. JAMA Ophthalmol. 2017 Feb 09 [PMID: 28196199]
Kaneko Y, Pappas C, Tajiri N, Borlongan CV. Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro. Sci Rep 2016 Oct 21 [PMID: 27767042]
Uggenti C, Briant K, Streit AK et al. Restoration of mutant bestrophin-1 expression, localisation and function. Dis Model Mech. Aug 12 2016 [PMID: 27519691] (WB,ICC/IF, Canine, Human) WB,ICC/IF Canine, Human
Cho MS, Kim SJ, Ku SY et al. Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses Stem Cell Res 2012 May 16 [PMID: 22683799] (ICC/IF, Human) ICC/IF Human
Ramachandra Rao S. Studies On The Role Of Sterol And Non-Sterol Isoprenoids In Retinal Health And Disease. Thesis. Jul 10 1905 (WB, Rat) WB Rat
Ukrohne TU, Westenskow PD, Kurihara T et al. Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells. Stem Cells Transl Med 2012 Feb [PMID: 22532929] (ICC/IF, Human) ICC/IF Human
Show All 29 Publications.

Review for Bestrophin 1 Antibody (NB300-164) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.

Reviews using NB300-164:
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Images Ratings Applications Species Date Details
Western Blot Bestrophin 1 NB300-164
reviewed by:
WB Human and Rat 12/29/2016


ApplicationWestern Blot
Sample TestedRPE whole cell lysate
SpeciesHuman and Rat


CommentsE6-6 used at 1:2000 over night. next day secondary antibody donkey anti-mouse peroxidase was applied followed by detection using enhanced chemiluminescence.

Product General Protocols

Video Protocols

WB Video Protocol

FAQs for Bestrophin 1 Antibody (NB300-164). (Showing 1 - 4 of 4 FAQs).

  1. According to the data sheet, for Immunohistochemistry, it references (PMID: 21249129). I see a 1:25 dilution in the journal article referenced under immunocytochemistry. Is this the same dilution recommended for IHC? If not do you have a recommended dilution? Antigen retrieval: Do you have any information on AR? Citrate buffer or higher pH (EDTA)? Temperature? Time? Incubation: Is 1 hour at room temperature or overnight at 4C recommended?
    • This antibody was validated in IHC in the reference PMID 11050159. Generally we recommend using antigen retrieval with citrate buffer and incubating for 1 hour at RT. Some antigens may be tricky and require overnight incubation. I imagine the specific protocol can be found in the reference, however.
  2. Can I get the recipe for RIPA buffer and the lysate preparation protocol for membrane bound proteins using NB300-164?
    • Here is both the recipe we use for RIPA and a good protocol for tissue lysate prep. RIPA buffer: 150 mM sodium chloride 1.0% NP-40 or Triton X-100 0.5% sodium deoxycholate 0.1% SDS (sodium dodecyl sulphate) 50 mM Tris, pH 8.0 RIPA buffer is also useful for whole cell extracts and membrane-bound proteins, and may be preferable to NP-40 or Triton X100-only buffers for extracting nuclear proteins. It will disrupt protein-protein interactions and may therefore be problematic for immunoprecipitations/pull down assays. Preparation of lysate from tissues Dissect the tissue of interest with clean tools, on ice preferably, and as quickly as possible to prevent degradation by proteases. Place the tissue in round-bottom microfuge tubes or Eppendorf tubes and immerse in liquid nitrogen to snap freeze. Store samples at -80C for later use or keep on ice for immediate homogenization. For a approximately 5 mg piece of tissue, add approximately 300 ul lysis buffer rapidly to the tube, homogenize with an electric homogenizer, rinse the blade twice with another 2x300 ul lysis buffer, then maintain constant agitation for 2 hours at 4C (e.g place on an orbital shaker in the fridge). Volumes of lysis buffer must be determined in relation to the amount of tissue present (protein extract should not be too diluted to avoid loss of protein and large volumes of samples to be loaded onto gels. The minimum concentration is 0.1 mg/ml, optimal concentration is 1-5 mg/ml). Centrifuge for 20 min at 12000 rpm at 4C in a microcentrifuge. Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice; discard the pellet.
  3. I want to know the concentration of Bestrophin 1 antibody [NB300-164].
    • For our product NB300-164, this antibody is provided as unpurified ascites. Therefore, the antibody concentration has not been determined.
  4. We are interested in your item, NB300-164 and would you please let us know the antibody concentration of it?
    • The total protein concentration of the ascites (Lot# B-3) is 11.7 mg/ml.

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Blogs on Bestrophin 1.

Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

Bestrophin 1: Implications in Progressive Vision Loss
The human Bestrophin family has four members, Best1, Best2, Best3 and Best4. These transmembrane proteins can function as chloride channels, and can also regulate calcium channels (1). The Bestrophins all have a conserved domain which begins at the N-...  Read full blog post.

"I can see clearly now": Targeting Bestrophin 1 to treat Bestrophinopathies
Encoded by the VMD2 gene on chromosome 11q13 Bestrophin 1 is the prototypic member of the RFP family of proteins which are more commonly called "bestrophins". The protein family was originally identified in Caenorhabditis elegans based on a conserved...  Read full blog post.

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Recent Reviews


Application: WB
Species: Human and Rat


Gene Symbol BEST1