Bestrophin 1 Antibody (E6-6) - BSA Free


Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa more
Immunocytochemistry/ Immunofluorescence: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - X-Z confocal single image scan of transiently transfected cells with different BEST1 cDNA constructs showing mislocalization more
Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 more
Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Detection of Bestrophin (68 kDa) from human RPE cell lysate.
Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Bestrophin 1 (E6-6) more
Genetic Strategies: Knockout Validated: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Immunoblotting showing the expression of RPE-specific proteins BEST1 (NB300-164), RPE65 (NB100-355), CRALBP, and the more
Dual RNAscope ISH-IHC: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Bestrophin 1 mRNA (ACD RNAScope Probe, catalog # 433181; more

Product Details

Reactivity Hu, Mu, Po, Ca, Pm, Rt(-)Species Glossary
Applications WB, Func, ICC/IF, IHC, IP, Dual ISH-IHC, KO
BSA Free
1.0 mg/ml
Validated by:

Genetic Strategies


Order Details

Bestrophin 1 Antibody (E6-6) - BSA Free Summary

Synthetic peptide conjugated to KLH corresponding to the C-terminus of human Bestrophin 1 (KDHMDPYWALENRDEAHS) [Uniprot: O76090]
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry reported in scientific literature (PMID 30048622)
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 24345323)
  • Immunoprecipitation
  • Knockout Validated
  • Proximity Ligation Assay reported in scientific literature (PMID 27519691)
  • Western Blot 1:1000
Application Notes
In Western blot, this antibody recognizes a band at ~68 kDa representing Bestrophin. Please see protocol for treatment of cell extracts. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NB300-164 in the following applications:

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NB300-164 in the following applications:

Reactivity Notes

Human, Primate, Porcine reactivity reported in scientific literature (PMID: 11050159). Use in Mouse reported in scientific literature (PMID:32791386).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein A or G purified

Alternate Names for Bestrophin 1 Antibody (E6-6) - BSA Free

  • ARB
  • Best disease
  • BEST
  • bestrophin 1
  • bestrophin-1
  • BMD
  • TU15B
  • vitelliform macular dystrophy 2
  • Vitelliform macular dystrophy protein 2
  • VMD2RP50


Best macular dystrophy (BMD) or vitelliform macular dystrophy (VMD2), is an autosomal form of macular degeneration, characterized by a depressed light peak in the electrooculogram (EOG). It is inherited and has an early onset. Bestrophin is a 68 kDa basolateral plasma membrane protein encoded by the VMD2 gene. Bestrophin's function is still unknown, but data suggests that it is a chloride channel that plays a role in generating the altered EOG in Best disease patients. In addition, Bestrophin is a useful biochemical and histological marker of RPE (retinal pigment epithelial cells) cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bestrophin 1 Antibody (NB300-164)(47)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Porcine, Primate.

We have publications tested in 7 applications: ICC/IF, IF/ICC, IF/IHC, IHC-Fr, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 47. Show All 47 Publications.
Publications using NB300-164 Applications Species
Li J, Qiu C, Wei Y et al. Human Amniotic Epithelial Stem Cell-Derived Retinal Pigment Epithelium Cells Repair Retinal Degeneration Frontiers in Cell and Developmental Biology 2021-09-28 [PMID: 34650985]
Sripathi SR, Hu MW, Turaga RC Et al. Epithelial to mesenchymal transition (EMT) of human stem cell-derived retinal pigment epithelium shares commonalities with malignancy-associated EMT: a proteomic analysis Molecular & cellular proteomics : MCP 2021-08-26 [PMID: 34455105] (WB, Human) WB Human
Afanasyeva TAV, Corral-Serrano JC, Garanto A et al. A look into retinal organoids: methods, analytical techniques, and applications Cellular and Molecular Life Sciences 2021-10-01 [PMID: 34420069]
Schustak J, Twarog M, Wu X et al. Mechanism of Nucleic Acid Sensing in Retinal Pigment Epithelium (RPE): RIG-I Mediates Type I Interferon Response in Human RPE Journal of Immunology Research 2021-06-18 [PMID: 34239945] (ICC/IF, Human) ICC/IF Human
Choudhury R, Bayatti N, Scharff R Et Al. FHL-1 interacts with human RPE cells through the alpha 5 beta 1 integrin and confers protection against oxidative stress Scientific reports 2021-07-08 [PMID: 34239032] (ICC/IF) ICC/IF
Zhao Q, Kong Y, Kittredge A et al. Distinct expression requirements and rescue strategies for BEST1 loss- and gain-of-function mutations eLife 2021-06-01 [PMID: 34061021] (WB) WB
Tsai YT, Li Y, Ryu J et al. Impaired cholesterol efflux in retinal pigment epithelium of individuals with juvenile macular degeneration American journal of human genetics 2021-04-21 [PMID: 33909993]
D'Antonio-Chronowska, A, D'Antonio, M Et al. In vitro Differentiation of Human iPSC-derived Retinal Pigment Epithelium Cells (iPSC-RPE). Bio Protoc 2019-12-20 [PMID: 33654959] (WB, Mouse) WB Mouse
Biswas P, Borooah S, Matsui H et al. Detection and validation of novel mutations in MERTK in a simplex case of retinal degeneration using WGS and hiPSC-RPEs model Human mutation 2020-11-30 [PMID: 33252167]
Bonilha, V L, Bell, B A Et al. Cellular Changes in Retinas From Patients With BEST1 Mutations. Front Cell Dev Biol 2020-11-07 [PMID: 33154968] (ICC/IF) ICC/IF
Show All 47 Publications.

Review for Bestrophin 1 Antibody (NB300-164) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.

Reviews using NB300-164:
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Human and Rat
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Images Ratings Applications Species Date Details
Western Blot Bestrophin 1 NB300-164
reviewed by:
Verified Customer
WB Human and Rat 12/29/2016


ApplicationWestern Blot
Sample TestedRPE whole cell lysate
SpeciesHuman and Rat


CommentsE6-6 used at 1:2000 over night. next day secondary antibody donkey anti-mouse peroxidase was applied followed by detection using enhanced chemiluminescence.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bestrophin 1 Antibody (NB300-164). (Showing 1 - 4 of 4 FAQs).

  1. According to the data sheet, for Immunohistochemistry, it references (PMID: 21249129). I see a 1:25 dilution in the journal article referenced under immunocytochemistry. Is this the same dilution recommended for IHC? If not do you have a recommended dilution? Antigen retrieval: Do you have any information on AR? Citrate buffer or higher pH (EDTA)? Temperature? Time? Incubation: Is 1 hour at room temperature or overnight at 4C recommended?
    • This antibody was validated in IHC in the reference PMID 11050159. Generally we recommend using antigen retrieval with citrate buffer and incubating for 1 hour at RT. Some antigens may be tricky and require overnight incubation. I imagine the specific protocol can be found in the reference, however.
  2. Can I get the recipe for RIPA buffer and the lysate preparation protocol for membrane bound proteins using NB300-164?
    • Here is both the recipe we use for RIPA and a good protocol for tissue lysate prep. RIPA buffer: 150 mM sodium chloride 1.0% NP-40 or Triton X-100 0.5% sodium deoxycholate 0.1% SDS (sodium dodecyl sulphate) 50 mM Tris, pH 8.0 RIPA buffer is also useful for whole cell extracts and membrane-bound proteins, and may be preferable to NP-40 or Triton X100-only buffers for extracting nuclear proteins. It will disrupt protein-protein interactions and may therefore be problematic for immunoprecipitations/pull down assays. Preparation of lysate from tissues Dissect the tissue of interest with clean tools, on ice preferably, and as quickly as possible to prevent degradation by proteases. Place the tissue in round-bottom microfuge tubes or Eppendorf tubes and immerse in liquid nitrogen to snap freeze. Store samples at -80C for later use or keep on ice for immediate homogenization. For a approximately 5 mg piece of tissue, add approximately 300 ul lysis buffer rapidly to the tube, homogenize with an electric homogenizer, rinse the blade twice with another 2x300 ul lysis buffer, then maintain constant agitation for 2 hours at 4C (e.g place on an orbital shaker in the fridge). Volumes of lysis buffer must be determined in relation to the amount of tissue present (protein extract should not be too diluted to avoid loss of protein and large volumes of samples to be loaded onto gels. The minimum concentration is 0.1 mg/ml, optimal concentration is 1-5 mg/ml). Centrifuge for 20 min at 12000 rpm at 4C in a microcentrifuge. Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice; discard the pellet.
  3. I want to know the concentration of Bestrophin 1 antibody [NB300-164].
    • For our product NB300-164, this antibody is provided as unpurified ascites. Therefore, the antibody concentration has not been determined.
  4. We are interested in your item, NB300-164 and would you please let us know the antibody concentration of it?
    • The total protein concentration of the ascites (Lot# B-3) is 11.7 mg/ml.

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Blogs on Bestrophin 1.

Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

Bestrophin 1: Implications in Progressive Vision Loss
The human Bestrophin family has four members, Best1, Best2, Best3 and Best4. These transmembrane proteins can function as chloride channels, and can also regulate calcium channels (1). The Bestrophins all have a conserved domain which begins at the N-...  Read full blog post.

"I can see clearly now": Targeting Bestrophin 1 to treat Bestrophinopathies
Encoded by the VMD2 gene on chromosome 11q13 Bestrophin 1 is the prototypic member of the RFP family of proteins which are more commonly called "bestrophins". The protein family was originally identified in Caenorhabditis elegans based on a conserved ...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Human and Rat


Gene Symbol BEST1