SP1 Antibody

Images

 
Biological Strategies: Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified ...read more
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-232] - FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Affinity purified rabbit anti-Sp1 used at a dilution of 1:1,000 (1 ...read more
Western Blot: SP1 Antibody [NB600-232] - A. Whole cell lysate (50 ug for E; 10 ug for T) from HEK 293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

SP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 700 and 740 using the numbering given in entry XP_028606.4 (GeneID 6667).
Localization
Nuclear
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 1 - 4 ug/mg of lysate
  • Western Blot 1:10000 - 1:25000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Chip, WB reactivity reported in scientific literature (PMID: 22402494).
Reviewed Applications
Read 1 Review rated 5
using
NB600-232 in the following applications:

Publications
Read Publications using
NB600-232 in the following applications:

  • 2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SP1 Antibody

  • Sp1 transcription factor
  • specificity protein 1
  • transcription factor Sp1
  • TSFP1

Background

Sp1 is one of the first cellular transcription factors to be identified, cloned and characterized for its ability to bind to GC-boxes in regulatory promoter elements. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, ChIP, IHC, IHC-P, IP

Publications for SP1 Antibody (NB600-232)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ChIP, WB.


Filter By Application
ChIP
(2)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB600-232 Applications Species
Bell CR, Pelly VS, Moeini A et al. Chemotherapy-induced COX-2 upregulation by cancer cells defines their inflammatory properties and limits the efficacy of chemoimmunotherapy combinations Nature communications Apr 19 2022 [PMID: 35440553] (WB, Mouse) WB Mouse
Yin Q, Chang H, Shen Q, Xing D Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis Journal of cellular and molecular medicine May 5 2021 [PMID: 33951300]
Kwon SM, Min S, Jeoun UW et al. Global spliceosome activity regulates entry into cellular senescence FASEB journal : official publication of the Federation of American Societies for Experimental Biology Jan 1 2021 [PMID: 33337569] (ChIP, Human) ChIP Human
Koizume S, Ito S, Miyagi E et al. HIF2 alpha-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cells Nucleic Acids Res 2012 Mar 8 [PMID: 22402494] (ChIP, WB, Human) ChIP, WB Human
Wu JB, Chen K, Li Y et al. Regulation of monoamine oxidase A by the SRY gene on the Y chromosome. FASEB J 2009 Nov [PMID: 19661285]
Wu JB, Chen K, Ou XM et al. Retinoic acid activates monoamine oxidase B promoter in human neuronal cells. J Biol Chem 2009 Jun [PMID: 19401466]
Gui J, Song Y, Han NL et al. Involvement of the GC-rich sequence and specific proteins (Sp1/Sp3) in the basal transcription activity of neurogranin gene. Biochem Biophys Res Commun 2006 Jun [PMID: 16677608]

Review for SP1 Antibody (NB600-232) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.

Reviews using NB600-232:
Filter by Applications
Western Blot
(1)
All Applications
Filter by Species
Human and Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SP1 NB600-232
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human and Rat 05/26/2020
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate,Vascular Smooth Muscle Cells
SpeciesHuman and Rat
LotA1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SP1 Antibody (NB600-232) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SP1 Products

Array NB600-232

Bioinformatics Tool for SP1 Antibody (NB600-232)

Discover related pathways, diseases and genes to SP1 Antibody (NB600-232). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SP1 Antibody (NB600-232)

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Pathways for SP1 Antibody (NB600-232)

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Research Areas for SP1 Antibody (NB600-232)

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Blogs on SP1.

Nur77 Activation and Tumor Suppression
Nur77 is a member of the steroid/thyroid hormone phosphoprotein receptor superfamily. It is heavily post-translationally modified and rapidly induced in response to androgens and growth factors. It governs fundamental processes such as cell proliferat...  Read full blog post.

Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration
By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

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Recent Reviews

5
5
1
4
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3
0
2
0
1
0

Verified Customer
05/26/2020
Application: Western Blot
Species: Human and Rat

Bioinformatics

Gene Symbol SP1
Entrez
Uniprot