Aggrecan Neoepitope Antibody - BSA Free

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Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in L929 whole cell extract using NB100-74350.
Immunocytochemistry/ Immunofluorescence: Aggrecan Neoepitope Antibody [NB100-74350] - Detection of ACAN in OA cartilage using NB100-74350.
Immunohistochemistry: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.
Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in U2OS cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Aggrecan Neoepitope Antibody - BSA Free Summary

Immunogen
A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]
Specificity
Aggrecan Neo (JSCNIT)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACAN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 21482542)
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 21482542)
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
Application Notes
In WB a band is detected at ~61 kDa representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins.
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Aggrecan Neoepitope Protein (NB100-74350PEP)
Publications
Read Publications using
NB100-74350 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aggrecan Neoepitope Antibody - BSA Free

  • AGC1SEDK
  • aggrecan core protein
  • aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPCP
  • CSPG1aggrecan 1
  • CSPGCP
  • large aggregating proteoglycan
  • MSK16AGCAN

Background

Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to the aggrecan/versican proteoglycan family and is a major component of the extracellular matrix of cartilagenous tissues. ACAN proteoglycan mainly consists of chondroitin sulfate, however it also contains keratan sulfate chains and N/O-linked oligosaccharides. Aggrecanase mediated cleavage results in the release of ACAN fragments from articular cartilage into synovial fluid during different stages of human osteoarthritis. ACAN is a secreted protein found in the extracellular space/extracellular matrix and is more or less restricted to cartilages where it interacts with FBLN1 as well as COM for serving the role of resisting compression in cartilage. ACAN binds to hyaluronic acid via an N-terminal globular region with high avidity and its expression occurs in chondrocytes throughout the developing skeleton. ACAN defects have been linked to spondyloepiphyseal dysplasia type Kimberley (SEDK), spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN), and osteochondritis dissecans short stature and early-onset osteoarthritis (OD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aggrecan Neoepitope Antibody (NB100-74350)(29)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 7 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(4)
ICC/IF
(3)
IF/IHC
(7)
IHC
(2)
IHC-Fr
(1)
IHC-P
(4)
WB
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(11)
Rat
(3)
Porcine
(4)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB100-74350 Applications Species
Song J, Kim EH, Yang JH et al. Upregulated FOXM1 stimulates chondrocyte senescence in Acot12-/-Nudt7-/- double knockout mice Theranostics 2023-09-25 [PMID: 37908734] (IHC-P, Mouse) IHC-P Mouse
Dong R, Xu H, Wang P et al. Disruption of Col9a2 expression leads to defects in osteochondral homeostasis and osteoarthritis-like phenotype in mice Journal of Orthopaedic Translation 2023-08-14 [PMID: 37635809] (IHC, ICC/IF) IHC, ICC/IF
Xiong B, Chen L, Huang Y et al. ZBTB16 eases lipopolysaccharide?elicited inflammation, apoptosis and degradation of extracellular matrix in chondrocytes during osteoarthritis by suppressing GRK2 transcription Experimental and Therapeutic Medicine 2023-04-24 [PMID: 37206562] (WB) WB
Cui S, Li W, Teixeira G et al. Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration JOR SPINE 2022-07-06 [PMID: 36203866]
Xu H, Dong R, Zeng Q et al. Col9a2 gene deletion accelerates the degeneration of intervertebral discs Experimental and Therapeutic Medicine 2022-01-07 [PMID: 35126710] (ICC/IF, IF/IHC, Mouse) ICC/IF, IF/IHC Mouse
Liu NQ, Lin Y, Li L et al. gp130/STAT3 signaling is required for homeostatic proliferation and anabolism in postnatal growth plate and articular chondrocytes Communications Biology 2022-01-17 [PMID: 35039652] (IHC) IHC
Oh Hk, Park M, Choi Sw Et Al. Suppression of Osteoarthritis progression by post-natal Induction of Nkx3.2 Biochemical and biophysical research communications 2021-07-27 [PMID: 34330063]
Ding Z, Qiu M, Alharbi M et al FOXO1 expression in chondrocytes modulates cartilage production and removal in fracture healing Bone 2021-03-04 [PMID: 33662610] (FLOW, Mouse)

Details:
Citation using the Alexa Fluor 488 version of this antibody.
FLOW Mouse
Ouhaddi Y, Najar M, Paré F, et al. L-PGDS deficiency accelerated the development of naturally occurring age-related osteoarthritis Aging 2020-12-23 [PMID: 33361529] (IHC-P, Mouse) IHC-P Mouse
Hozain S, Hernandez A, Fuller J, et al. Zinc chloride affects chondrogenesis via VEGF signaling Experimental cell research 2021-02-15 [PMID: 33358860] (FLOW, Mouse) FLOW Mouse
Show All 29 Publications.

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Product General Protocols

View specific protocols for Aggrecan Neoepitope Antibody (NB100-74350): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aggrecan Neoepitope Antibody (NB100-74350). (Showing 1 - 1 of 1 FAQs).

  1. I found the following products on your webpage: Aggrecan Neoepitope Antibody (NB100-74350), Aggrecan Neoepitope Antibody [HRP] (NB100-74350H), and Aggrecan Neoepitope Antibody [Biotin] (NB100-74350B). It is clear that both tagged antibodies are directed against the NITEGE neoepitope of aggrecan, but it is not clear for the non tagged antibody. Looking at the datasheets, those three antibodies look quite the same. Can you confirm that the non tagged antibody is also directed against the NITEGE neoepitope? The immunogen indicated in the datasheet is "A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400)"
    • NB100-74350 is the same antibody that is conjugated to make NB100-74350B and NB100-74350H. The immunogen for NB100-74350 is the same as NB100-74350B and NB100-74350H.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACAN
Entrez
Uniprot