Aggrecan Neoepitope Antibody

Images

 
Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in L929 whole cell extract using NB100-74350.
Immunocytochemistry/ Immunofluorescence: Aggrecan Neoepitope Antibody [NB100-74350] - Detection of ACAN in OA cartilage using NB100-74350.
Immunohistochemistry: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.
Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in U2OS cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Aggrecan Neoepitope Antibody Summary

Immunogen
A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]
Specificity
Aggrecan Neo (JSCNIT)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACAN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
This Aggrecan Neoepitope antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western Blot. In WB a band is detected at ~61 kDa representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins. Use in Immunohistochemistry-Frozen and Flow Cytometry reported in scientific literature (PMID 21482542)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Aggrecan Neoepitope Peptide (NB100-74350PEP)
Publications
Read Publications using
NB100-74350 in the following applications:

Reactivity Notes

Porcine, Bovine, Canine, Rat, Mouse, and Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aggrecan Neoepitope Antibody

  • AGC1SEDK
  • aggrecan core protein
  • aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPCP
  • CSPG1aggrecan 1
  • CSPGCP
  • large aggregating proteoglycan
  • MSK16AGCAN

Background

Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to aggrecan/versican proteoglycan family and is a major component of extracellular matrix of cartilagenous tissues. ACAN proteoglycan is mainly consists of chondroitin sulfate, however, it also contains keratan sulfate chains, N/O-linked oligosaccharides. Aggrecanase mediated cleavage results in ACAN fragments release from articular cartilage into synovial fluid during different stages of human osteoarthritis. ACAN is a secreted protein found in extracellular space/extracellular matrix and is more or less restricted to cartilages where it interact with FBLN1 as well as COM for serving the role of resisting compression in cartilage. ACAN binds to hyaluronic acid via an N-terminal globular region with high avidity and its expression occurs in chondrocytes throughout the developing skeleton. ACAN defects have been linked to spondyloepiphyseal dysplasia type Kimberley (SEDK), spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN), osteochondritis dissecans short stature and early-onset osteoarthritis (OD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aggrecan Neoepitope Antibody (NB100-74350)(6)

We have publications tested in 3 confirmed species: Mouse, Rat, Porcine.

We have publications tested in 5 applications: FLOW, IHC, IHC-Fr, IHC-P, WB.


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FLOW
(1)
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
WB
(1)
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Mouse
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Rat
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Porcine
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Showing Publications 1 - 6 of 6.
Publications using NB100-74350 Applications Species
Wang Y, Yu D, Liu Z et al. Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix Stem Cell Res Ther 2017 Aug 14 [PMID: 28807034] (IHC-P, Mouse) IHC-P Mouse
Lai JH, Luo SF, Hung LF et al. Physiological concentrations of soluble uric acid are chondroprotective and anti-inflammatory. Sci Rep May 24 2017 [PMID: 28539647] (WB, Porcine) WB Porcine
Hill Adele, Duran Juanita, Purcell Patricia. Lubricin protects the temporomandibular joint surfaces from degeneration. PLoS One. 2014 Sep 04 [PMID: 25188282] (IHC) IHC
Liu FC, Huang HS, Huang CY, Yang R, Chang DM, Lai JH, Ho LJ. A benzamide-linked small molecule HS-Cf inhibits TNF-?-induced interferon regulatory factor-1 in Porcine chondrocytes: a potential disease-modifying drug for osteoarthritis therapeutics. J Clin Immunol;31(6):1131-42. 2011 Dec. [PMID: 21858617] (IHC, Porcine) IHC Porcine
Huang CY, Lai KY, Hung LF et al. Advanced glycation end products cause collagen II reduction by activating Janus kinase/signal transducer and activator of transcription 3 pathway in porcine chondrocytes. Rheumatology (Oxford). 2011 Aug [PMID: 21482542] (FLOW, IHC-Fr, Porcine) FLOW, IHC-Fr Porcine
Juneja SC, Ventura M, Jay GD, Veillette C. A Less Invasive Approach of Medial Meniscectomy in Rat: A Model to Target Early or Less Severe Human Osteoarthritis. J Arthritis. May 27 2016 (IHC-P, Rat) IHC-P Rat

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Product General Protocols

View specific protocols for Aggrecan Neoepitope Antibody (NB100-74350): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aggrecan Neoepitope Antibody (NB100-74350). (Showing 1 - 1 of 1 FAQs).

  1. I found the following products on your webpage: Aggrecan Neoepitope Antibody (NB100-74350), Aggrecan Neoepitope Antibody [HRP] (NB100-74350H), and Aggrecan Neoepitope Antibody [Biotin] (NB100-74350B). It is clear that both tagged antibodies are directed against the NITEGE neoepitope of aggrecan, but it is not clear for the non tagged antibody. Looking at the datasheets, those three antibodies look quite the same. Can you confirm that the non tagged antibody is also directed against the NITEGE neoepitope? The immunogen indicated in the datasheet is "A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400)"
    • NB100-74350 is the same antibody that is conjugated to make NB100-74350B and NB100-74350H. The immunogen for NB100-74350 is the same as NB100-74350B and NB100-74350H.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-74350AF405
Alexa Fluor 488 Labeled NB100-74350AF488
Alexa Fluor 647 Labeled NB100-74350AF647
Alexa Fluor 700 Labeled NB100-74350AF700
Biotin Labeled NB100-74350B
DyLight 405 Labeled NB100-74350V
DyLight 488 Labeled NB100-74350G
DyLight 550 Labeled NB100-74350R
DyLight 650 Labeled NB100-74350C
FITC Labeled NB100-74350F
HRP Labeled NB100-74350H

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Bioinformatics

Gene Symbol ACAN
Entrez
Uniprot