Aggrecan Neoepitope Antibody


Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in L929 whole cell extract using NB100-74350.
Immunocytochemistry/ Immunofluorescence: Aggrecan Neoepitope Antibody [NB100-74350] - Detection of ACAN in OA cartilage using NB100-74350.
Immunohistochemistry: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.
Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in U2OS cell lysate.

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
1 mg/ml

Aggrecan Neoepitope Antibody Summary

A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]
Aggrecan Neo (JSCNIT)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This Aggrecan Neoepitope antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western Blot. In WB a band is detected at ~61 kDa representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins. Use in Immunohistochemistry-Frozen and Flow Cytometry reported in scientific literature (PMID 21482542)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
61 kDa
Control Peptide
Aggrecan Neoepitope Peptide (NB100-74350PEP)
Read Publications using
NB100-74350 in the following applications:

Reactivity Notes

Porcine, Bovine, Canine, Rat, Mouse, and Human.

Alternate Names for Aggrecan Neoepitope Antibody

  • aggrecan core protein
  • aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPG1aggrecan 1
  • large aggregating proteoglycan


Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to aggrecan/versican proteoglycan family and is a major component of extracellular matrix of cartilagenous tissues. ACAN proteoglycan is mainly consists of chondroitin sulfate, however, it also contains keratan sulfate chains, N/O-linked oligosaccharides. Aggrecanase mediated cleavage results in ACAN fragments release from articular cartilage into synovial fluid during different stages of human osteoarthritis. ACAN is a secreted protein found in extracellular space/extracellular matrix and is more or less restricted to cartilages where it interact with FBLN1 as well as COM for serving the role of resisting compression in cartilage. ACAN binds to hyaluronic acid via an N-terminal globular region with high avidity and its expression occurs in chondrocytes throughout the developing skeleton. ACAN defects have been linked to spondyloepiphyseal dysplasia type Kimberley (SEDK), spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN), osteochondritis dissecans short stature and early-onset osteoarthritis (OD).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aggrecan Neoepitope Antibody (NB100-74350)(4)

We have publications tested in 2 confirmed species: Rat, Porcine.

We have publications tested in 4 applications: FLOW, IHC, IHC-Fr, IHC-P.

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Product General Protocols

View specific protocols for Aggrecan Neoepitope Antibody (NB100-74350): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aggrecan Neoepitope Antibody (NB100-74350). (Showing 1 - 1 of 1 FAQs).

  1. I found the following products on your webpage: Aggrecan Neoepitope Antibody (NB100-74350), Aggrecan Neoepitope Antibody [HRP] (NB100-74350H), and Aggrecan Neoepitope Antibody [Biotin] (NB100-74350B). It is clear that both tagged antibodies are directed against the NITEGE neoepitope of aggrecan, but it is not clear for the non tagged antibody. Looking at the datasheets, those three antibodies look quite the same. Can you confirm that the non tagged antibody is also directed against the NITEGE neoepitope? The immunogen indicated in the datasheet is "A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400)"
    • NB100-74350 is the same antibody that is conjugated to make NB100-74350B and NB100-74350H. The immunogen for NB100-74350 is the same as NB100-74350B and NB100-74350H.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-74350AF405
Alexa Fluor (R) 488 Labeled NB100-74350AF488
Alexa Fluor (R) 647 Labeled NB100-74350AF647
Alexa Fluor (R) 700 Labeled NB100-74350AF700
Biotin Labeled NB100-74350B
DyLight 405 Labeled NB100-74350V
DyLight 488 Labeled NB100-74350G
DyLight 550 Labeled NB100-74350R
DyLight 650 Labeled NB100-74350C
FITC Labeled NB100-74350F
HRP Labeled NB100-74350H

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Contact Information

Product PDFs


Gene Symbol ACAN

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