Aggrecan Neoepitope Antibody

Images

 
Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in L929 whole cell extract using NB100-74350.
Immunocytochemistry/ Immunofluorescence: Aggrecan Neoepitope Antibody [NB100-74350] - Detection of ACAN in OA cartilage using NB100-74350.
Immunohistochemistry: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.
Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in U2OS cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Aggrecan Neoepitope Antibody Summary

Immunogen
A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]
Specificity
Aggrecan Neo (JSCNIT)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACAN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
This Aggrecan Neoepitope antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western Blot. In WB a band is detected at ~61 kDa representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins. Use in Immunohistochemistry-Frozen and Flow Cytometry reported in scientific literature (PMID 21482542)
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Publications
Read Publications using
NB100-74350 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aggrecan Neoepitope Antibody

  • AGC1SEDK
  • aggrecan core protein
  • aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPCP
  • CSPG1aggrecan 1
  • CSPGCP
  • large aggregating proteoglycan
  • MSK16AGCAN

Background

Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to the aggrecan/versican proteoglycan family and is a major component of the extracellular matrix of cartilagenous tissues. ACAN proteoglycan mainly consists of chondroitin sulfate, however it also contains keratan sulfate chains and N/O-linked oligosaccharides. Aggrecanase mediated cleavage results in the release of ACAN fragments from articular cartilage into synovial fluid during different stages of human osteoarthritis. ACAN is a secreted protein found in the extracellular space/extracellular matrix and is more or less restricted to cartilages where it interacts with FBLN1 as well as COM for serving the role of resisting compression in cartilage. ACAN binds to hyaluronic acid via an N-terminal globular region with high avidity and its expression occurs in chondrocytes throughout the developing skeleton. ACAN defects have been linked to spondyloepiphyseal dysplasia type Kimberley (SEDK), spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN), and osteochondritis dissecans short stature and early-onset osteoarthritis (OD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aggrecan Neoepitope Antibody (NB100-74350)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 5 applications: FLOW, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(1)
IHC
(5)
IHC-Fr
(1)
IHC-P
(2)
WB
(2)
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Human
(2)
Mouse
(3)
Rat
(1)
Porcine
(4)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-74350 Applications Species
Ho YJ, Lu JW, Ho LJ et al. Anti-inflammatory and anti-osteoarthritis effects of Cm-02 and Ck-02 Biochem. Biophys. Res. Commun. Jul 25 2019 [PMID: 31353084]
Xia C, Wang P, Fang L et al. Activation of beta-catenin in Col2-expressing chondrocytes leads to osteoarthritis-like defects in hip joint J. Cell. Physiol. Aug 1 2019 [PMID: 30912140] (IHC, Mouse) IHC Mouse
Song J, Baek IJ, Chun CH et al. Dysregulation of the NUDT7-PGAM1 axis is responsible for chondrocyte death during osteoarthritis pathogenesis Nat Commun Aug 24 2018 [PMID: 30143643] (IHC, Human) IHC Human
Shkhyan R, Lee S, Gullo F et al. Genetic ablation of adenosine receptor A3 results in articular cartilage degeneration J. Mol. Med. Aug 7 2018 [PMID: 30088034] (WB, Porcine) WB Porcine
Liu FC, Lu JW, Chien CY et al. Arthroprotective Effects of Cf-02 Sharing Structural Similarity with Quercetin Int J Mol Sci May 14 2018 [PMID: 29757957] (IHC, Human) IHC Human
Pan Z, Sun H, Xie B et al. Therapeutic effects of gefitinib-encapsulated thermosensitive injectable hydrogel in intervertebral disc degeneration Biomaterials 2018 Jan 01 [PMID: 29396379] (Mouse) Mouse
Wang Y, Yu D, Liu Z et al. Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix Stem Cell Res Ther 2017 Aug 14 [PMID: 28807034] (IHC-P, Mouse) IHC-P Mouse
Lai JH, Luo SF, Hung LF et al. Physiological concentrations of soluble uric acid are chondroprotective and anti-inflammatory. Sci Rep May 24 2017 [PMID: 28539647] (WB, Porcine) WB Porcine
Juneja SC, Ventura M, Jay GD, Veillette C. A Less Invasive Approach of Medial Meniscectomy in Rat: A Model to Target Early or Less Severe Human Osteoarthritis. J Arthritis. May 27 2016 (IHC-P, Rat) IHC-P Rat
Hill Adele, Duran Juanita, Purcell Patricia. Lubricin protects the temporomandibular joint surfaces from degeneration. PLoS One. 2014 Sep 04 [PMID: 25188282] (IHC) IHC
Show All 12 Publications.

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Product General Protocols

View specific protocols for Aggrecan Neoepitope Antibody (NB100-74350): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aggrecan Neoepitope Antibody (NB100-74350). (Showing 1 - 1 of 1 FAQs).

  1. I found the following products on your webpage: Aggrecan Neoepitope Antibody (NB100-74350), Aggrecan Neoepitope Antibody [HRP] (NB100-74350H), and Aggrecan Neoepitope Antibody [Biotin] (NB100-74350B). It is clear that both tagged antibodies are directed against the NITEGE neoepitope of aggrecan, but it is not clear for the non tagged antibody. Looking at the datasheets, those three antibodies look quite the same. Can you confirm that the non tagged antibody is also directed against the NITEGE neoepitope? The immunogen indicated in the datasheet is "A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400)"
    • NB100-74350 is the same antibody that is conjugated to make NB100-74350B and NB100-74350H. The immunogen for NB100-74350 is the same as NB100-74350B and NB100-74350H.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-74350AF350
Alexa Fluor 405 NB100-74350AF405
Alexa Fluor 488 NB100-74350AF488
Alexa Fluor 532 NB100-74350AF532
Alexa Fluor 594 NB100-74350AF594
Alexa Fluor 647 NB100-74350AF647
Alexa Fluor 700 NB100-74350AF700
Alexa Fluor 750 NB100-74350AF750
Allophycocyanin/Cy7 NB100-74350APCCY7
Biotin NB100-74350B
DyLight 350 NB100-74350UV
DyLight 405 NB100-74350V
DyLight 488 NB100-74350G
DyLight 550 NB100-74350R
DyLight 594 NB100-74350DL594
DyLight 650 NB100-74350C
DyLight 680 NB100-74350FR
DyLight 755 NB100-74350IR
FITC NB100-74350F
HRP NB100-74350H
Janelia Fluor 549 NB100-74350JF549
Janelia Fluor 646 NB100-74350JF646
PE/Atto594 NB100-74350PEATT594
PE/Cy5.5 NB100-74350PECY55
PE/Cy7 NB100-74350PECY7

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Bioinformatics

Gene Symbol ACAN
Entrez
Uniprot