Osteopontin/OPN Antibody [Unconjugated]

Images

 
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by ...read more
Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Osteopontin/OPN Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R&D Systems, Catalog # 441-OP)
Leu17-Asn294 (Glu99Gly)
Accession # Q547B5
Specificity
Detects mouse Osteopontin (OPN) in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human OPN is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPP1
Endotoxin Note
<0.30 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 1-3 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture:Mouse Osteopontin/OPN Antibody (Catalog # AF808)
ELISA Detection: Mouse Osteopontin/OPN Biotinylated Antibody (Catalog # BAF808)
Standard: Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF808 in the following applications:

Publications
Read Publications using
AF808 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteopontin/OPN Antibody [Unconjugated]

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

  1. Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21.
  2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Mu
Applications: WB, IHC, B/N, ELISA(Cap)

Publications for Osteopontin/OPN Antibody (AF808)(56)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 16 applications: ELISA Capture, ELISA Development, ICC, IHC, IHC - Not specified, IHC - PFA fixed, IHC Fluorescence, IHC Fresh, IHC Frozen, IHC Paraffin-embedded, IHC paraffin embedded, IHC-OCT embedded, In Vivo, In vivo, Neut, WB.


Filter By Application
ELISA Capture
(2)
ELISA Development
(1)
ICC
(3)
IHC
(12)
IHC - Not specified
(2)
IHC - PFA fixed
(1)
IHC Fluorescence
(1)
IHC Fresh
(1)
IHC Frozen
(5)
IHC Paraffin-embedded
(5)
IHC paraffin embedded
(1)
IHC-OCT embedded
(1)
In Vivo
(1)
In vivo
(4)
Neut
(4)
WB
(12)
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Filter By Species
Human
(1)
Mouse
(53)
Rat
(2)
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Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using AF808 Applications Species
AM D'Souza, Y Jiang, A Cast, L Valanejad, M Wright, K Lewis, M Kumbaji, S Shah, D Smithrud, R Karns, S Shin, N Timchenko Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation Cell Mol Gastroenterol Hepatol, 2018;6(3):239-255. 2018 [PMID: 30109252] (IHC,, Mouse) IHC Mouse
HA Himburg, CM Termini, L Schlussel, J Kan, M Li, L Zhao, T Fang, JP Sasine, VY Chang, JP Chute Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30100167] (IHC,, Mouse) IHC Mouse
Q Zeng, X Luo, M Han, W Liu, H Li Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity EBioMedicine, 2018;0(0):. 2018 [PMID: 29885866] (In vivo,, Mouse) In vivo Mouse
Y Wen, C Wang, J Gu, C Yu, K Wang, X Sun, Y Sun, H Wu, Y Tong, Q Xia, X Kong Metabolic modulation of acetaminophen-induced hepatotoxicity by osteopontin Cell. Mol. Immunol., 2018;0(0):. 2018 [PMID: 29735980] (IHC,, Mouse) IHC Mouse
KH Kim, SH Kim, DH Han, YS Jo, YH Lee, MS Lee Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice Sci Rep, 2018;8(1):6789. 2018 [PMID: 29717162] (IHC,, Mouse) IHC Mouse
J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes Tbr1 instructs laminar patterning of retinal ganglion cell dendrites Nat. Neurosci., 2018;0(0):. 2018 [PMID: 29632360] (IHC,, Mouse) IHC Mouse
W Ding, K Yousefi, S Goncalves, BJ Goldstein, AL Sabater, A Kloosterbo, P Ritter, G Lambert, AJ Mendez, LA Shehadeh Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits JCI Insight, 2018;3(6):. 2018 [PMID: 29563333] (WB,, Mouse) WB Mouse
C Hernandez, P Huebener, JP Pradere, DJ Antoine, RA Friedman, RF Schwabe HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29558367] (IHC,, Mouse) IHC Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC,, Mouse) IHC Mouse
VCA de Souza, TA Pereira, VW Teixeira, H Carvalho, MCAB de Castro, CG D'assunção, AF de Barros, CL Carvalho, VMB de Lorena, VMA Costa, ÁAC Teixeira, RCBQ Figueiredo, SA de Oliveir Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin, TGF-?1, IL-13 and oxidative stress World J. Gastroenterol., 2017;23(28):5146-5157. 2017 [PMID: 28811709] (IHC paraffin embedded,, Mouse) IHC paraffin embedded Mouse
Show All 56 Publications.

Reviews for Osteopontin/OPN Antibody (AF808) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF808:
Filter by Applications
IHC-P
(2)
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Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24038141
SpeciesMouse
 
reviewed by:
Anonymous
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20548025
SpeciesMouse

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Recent Reviews

4.5
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2
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Anonymous
01/09/2015
Application: IHC-P
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol SPP1
Uniprot