Osteopontin/OPN Antibody [Unconjugated]

Images

 
Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen ...read more
Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by ...read more
Notch ligands Jag1 and Dll1 are both required for segregation of hepatocytic fate centrally and biliary fate peripherally.(A) Immunolabeling for OPN and HNF4A of BMEL cells presented with DLL4 on 30 kPa substrates. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap), ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Osteopontin/OPN Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R&D Systems, Catalog # 441-OP)
Leu17-Asn294 (Glu99Gly)
Accession # Q547B5
Specificity
Detects mouse Osteopontin (OPN) in ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPP1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.30 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-3 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture:Mouse Osteopontin/OPN Antibody (Catalog # AF808)
ELISA Detection: Mouse Osteopontin/OPN Biotinylated Antibody (Catalog # BAF808)
Standard: Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF808 in the following applications:

Publications
Read Publications using
AF808 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteopontin/OPN Antibody [Unconjugated]

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

  1. Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21.
  2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Osteopontin/OPN Antibody (AF808)(125)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 17 applications: Bioassay, Cell Culture, ELISA Capture, ELISA Development, ELISA Development (Capture), Flow Cytometry, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, IHC/IF, In Vivo, Neutralization, RNAScope, Western Blot.


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Bioassay
(1)
Cell Culture
(2)
ELISA Capture
(1)
ELISA Development
(1)
ELISA Development (Capture)
(2)
Flow Cytometry
(1)
ICC
(14)
ICC/IF
(2)
IF
(1)
IHC
(54)
IHC-Fr
(10)
IHC-P
(14)
IHC/IF
(1)
In Vivo
(3)
Neutralization
(13)
RNAScope
(1)
Western Blot
(24)
All Applications
Filter By Species
Human
(3)
Mouse
(117)
Rat
(4)
Transgenic Mouse
(4)
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Showing Publications 1 - 10 of 125. Show All 125 Publications.
Publications using AF808 Applications Species
Zhao, M;Toma, K;Kinde, B;Li, L;Patel, AK;Wu, KY;Lum, MR;Tan, C;Hooper, JE;Kriegstein, AR;La Torre, A;Liao, YJ;Welsbie, DS;Hu, Y;Han, Y;Duan, X; Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy Cell reports 2023-08-23 [PMID: 37624696] (IHC, Mouse) IHC Mouse
Wang, X;Ramos, R;Phan, AQ;Yamaga, K;Flesher, JL;Jiang, S;Oh, JW;Jin, S;Jahid, S;Kuan, CH;Nguyen, TK;Liang, HY;Shettigar, NU;Hou, R;Tran, KH;Nguyen, A;Vu, KN;Phung, JL;Ingal, JP;Levitt, KM;Cao, X;Liu, Y;Deng, Z;Taguchi, N;Scarfone, VM;Wang, G;Paolilli, KN;Wang, X;Guerrero-Juarez, CF;Davis, RT;Greenberg, EN;Ruiz-Vega, R;Vasudeva, P;Murad, R;Widyastuti, LHP;Lee, HL;McElwee, KJ;Gadeau, AP;Lawson, DA;Andersen, B;Mortazavi, A;Yu, Z;Nie, Q;Kunisada, T;Karin, M;Tuckermann, J;Esko, JD;Ganesan, AK;Li, J;Plikus, MV; Signalling by senescent melanocytes hyperactivates hair growth Nature 2023-06-01 [PMID: 37344645] (IHC, Western Blot, Transgenic Mouse) IHC, Western Blot Transgenic Mouse
E Yildiz, G El Alam, A Perino, A Jalil, PD Denechaud, K Huber, L Fajas, J Auwerx, G Sorrentino, K Schoonjans Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs Elife, 2023-03-06;12(0):. 2023-03-06 [PMID: 36876915] (IHC, Mouse) IHC Mouse
L Li, L Cui, P Lin, Z Liu, S Bao, X Ma, H Nan, W Zhu, J Cen, Y Mao, X Ma, L Jiang, Y Nie, F Ginhoux, Y Li, H Li, L Hui Kupffer-cell-derived IL-6 is repurposed for hepatocyte dedifferentiation via activating progenitor genes from injury-specific enhancers Cell Stem Cell, 2023-02-13;0(0):. 2023-02-13 [PMID: 36787740] (IHC, Mouse) IHC Mouse
S De Scheppe, JZ Ge, G Crowley, LSS Ferreira, D Garceau, CE Toomey, D Sokolova, J Rueda-Carr, SH Shin, JS Kim, T Childs, T Lashley, JJ Burden, M Sasner, C Sala Frige, S Jung, S Hong Perivascular cells induce microglial phagocytic states and synaptic engulfment via SPP1 in mouse models of Alzheimer&#039;s disease Nature Neuroscience, 2023-02-06;0(0):. 2023-02-06 [PMID: 36747024] (IHC, Mouse) IHC Mouse
Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer&#039;s disease Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120. 2023-02-02 [PMID: 36730200] (Bioassay, IHC, Mouse) Bioassay, IHC Mouse
D Piekna-Prz, D Na, J Zhang, C Baker, JM Ashton, PM White Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation Frontiers in Cellular Neuroscience, 2023-01-06;16(0):1096872. 2023-01-06 [PMID: 36687526] (ICC, Mouse) ICC Mouse
D Nagashima, Y Ishibashi, S Kawaguchi, M Furukawa, M Toho, M Ohno, T Nitto, N Izumo Human Recombinant Lactoferrin Promotes Differentiation and Calcification on MC3T3-E1 Cells Pharmaceutics, 2022-12-25;15(1):. 2022-12-25 [PMID: 36678689] (ICC/IF, Mouse) ICC/IF Mouse
Q Bai, X Wang, H Yan, L Wen, Z Zhou, Y Ye, Y Jing, Y Niu, L Wang, Z Zhang, J Su, T Chang, G Dou, Y Wang, J Sun Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling Journal of personalized medicine, 2023-01-11;13(1):. 2023-01-11 [PMID: 36675807] (IHC, Neutralization, Mouse) IHC, Neutralization Mouse
YS Song, N Jamali, CM Sorenson, N Sheibani Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial Cells Cells, 2023-01-16;12(2):. 2023-01-16 [PMID: 36672270] (Western Blot, Mouse) Western Blot Mouse
Show All 125 Publications.

Reviews for Osteopontin/OPN Antibody (AF808) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF808:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Mouse 01/09/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24038141
SpeciesMouse
  4
reviewed by:
Verified Customer
IHC-P Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20548025
SpeciesMouse

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Verified Customer
01/09/2015
Application: IHC-P
Species: Mouse

 
Verified Customer
01/09/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol SPP1
Uniprot