Recombinant Human COCO Protein


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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human COCO Protein Summary

Details of Functionality
Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.15-0.75 µg/mL in the presence of 50 ng/mL of rhBMP-4.
E. coli-derived human COCO protein
Arg23-Ala189, with an N-terminal Met
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
18.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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3047-CC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human COCO Protein

  • CER2CRL2
  • Cerberus 2
  • Cerberus like-2
  • cerberus-like 2
  • Cerberus-like protein 2
  • CERL2
  • cerl-2
  • CKTSF1B3
  • CKTSF1B3MGC126849
  • COCO
  • Cysteine knot superfamily 1, BMP antagonist 3
  • DAN domain family member 5
  • DAN domain family, member 5
  • DAND5
  • Dante
  • DTE
  • FLJ38607
  • GREM3
  • GREM3cerberus 2
  • gremlin-3
  • SP1


COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN Domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1 - 3). BMPs play important roles in tissue morphogenesis and development processes (4, 5, 6). The human COCO cDNA encodes a 189 amino acid (aa) precursor with a 22 aa signal sequence (2, 7). COCO has eight Cys residues in the cysteine-knot which places it in the CAN subfamily of BMP antagonists along with the other DAN family proteins (1). Human COCO shares 60% and 24% aa sequence identity with mouse and Xenopus COCO, respectively. It shares 17%, 20%, 25%, and 22% aa sequence identity with human DAN, Gremlin, PRDC, and Cerberus, respectively. In Xenopus embryonal development, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO binds and inhibits Xnr1, BMP-4, Activin, and Wnt-8 (7). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8). COCO may cooperate with Nodal in gastrulation and embryonic left-right axis formation (5, 8).

  1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
  2. Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423.
  3. Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885.
  4. Baron, M.H. (2005) Exp. Hematol. 33:1015.
  5. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  6. De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285.
  7. Bell, E. et al. (2003) Development 130:1381.
  8. Pearce, J.J. et al. (1999) Dev. Biol. 209:98.

Publications for COCO/DAND5 (3047-CC)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, Cell Culture.

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Gene Symbol DAND5