Osteocalcin Antibody (190125)

Images

 
Saos‑2 human osteosarcoma cell line was stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more
Human osteoblasts were stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more
Osteocalcin was detected in human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Osteocalcin was detected in immersion fixed paraffin-embedded sections of human cartilage using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 8 µg/mL overnight at 4 °C. Tissue was stained ...read more
Osteocalcin was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 25 µg/mL overnight at 4 °C. Tissue was ...read more
Osteocalcin was detected in immersion fixed MG‑63 human osteosarcoma cell line using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
Osteocalcin was detected in immersion fixed rat osteocytes differentiated from mesenchymal stem cells using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications IHC, CyTOF-ready, Flow, ICC/IF
Clone
190125
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Osteocalcin Antibody (190125) Summary

Immunogen
Human Osteocalcin synthetic peptide
YLYQWLGAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV
Accession # P02818
Specificity
Detects human Osteocalcin in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BGLAP
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 2 Reviews rated 4
using
MAB1419 in the following applications:

Publications
Read Publications using
MAB1419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteocalcin Antibody (190125)

  • BGLAP
  • BGP
  • bone gamma-carboxyglutamate (gla) protein (osteocalcin)
  • bone gamma-carboxyglutamate (gla) protein
  • Bone Gla protein
  • Gamma-carboxyglutamic acid-containing protein
  • OC
  • OCN
  • Osteocalcin

Background

Osteocalcin, also known as Bone gamma -Carboxyglutamic Acid Protein, is a secreted protein whose expression is restricted to cells of the osteoblast lineage (1). It has been frequently used as a marker for osteoblast lineage cells.
  1. Lian, J.B. et al. (1999) Vitamin. Horm. 55:443.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, Flow, ICC/IF

Publications for Osteocalcin Antibody (MAB1419)(24)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P.


Filter By Application
Flow Cytometry
(1)
ICC
(10)
IHC
(10)
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter By Species
Human
(17)
Mouse
(1)
Rat
(5)
Canine
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using MAB1419 Applications Species
S Lv, J Xu, L Chen, H Wu, W Feng, Y Zheng, P Li, H Zhang, L Zhang, G Chi, Y Li MicroRNA-27b targets CBFB to inhibit differentiation of human bone marrow mesenchymal stem cells into hypertrophic chondrocytes Stem Cell Res Ther, 2020;11(1):392. 2020 [PMID: 32917285] (IHC, Rat) IHC Rat
J Chen, C Tu, X Tang, H Li, J Yan, Y Ma, H Wu, C Liu The combinatory effect of sinusoidal electromagnetic field and VEGF promotes osteogenesis and angiogenesis of mesenchymal stem cell-laden PCL/HA implants in a rat subcritical cranial defect Stem Cell Res Ther, 2019;10(1):379. 2019 [PMID: 31842985] (ICC, Rat) ICC Rat
V Miceli, M Pampalone, S Vella, AP Carreca, G Amico, PG Conaldi Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems Stem Cells Int, 2019;2019(0):7486279. 2019 [PMID: 30911299] (ICC, Human) ICC Human
AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment Breast Cancer Res., 2019;21(1):31. 2019 [PMID: 30813947] (ICC, Human) ICC Human
A Kapelouzou, C Kontogiann, DI Tsilimigra, G Georgiopou, L Kaklamanis, L Tsourelis, DV Cokkinos Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans Cytokine, 2019;116(0):150-160. 2019 [PMID: 30716659] (IHC, Human) IHC Human
X Gao, A Lu, Y Tang, J Schneppend, AB Liebowitz, AC Scibetta, ER Morris, H Cheng, C Huard, S Amra, B Wang, MA Hall, WR Lowe, J Huard Influences of donor and host age on human muscle-derived stem cell-mediated bone regeneration Stem Cell Res Ther, 2018;9(1):316. 2018 [PMID: 30463597] (IHC-Fr, Human) IHC-Fr Human
Y Zhu, Y Wu, J Cheng, Q Wang, Z Li, Y Wang, D Wang, H Wang, W Zhang, J Ye, H Jiang, L Wang Pharmacological activation of TAZ enhances osteogenic differentiation and bone formation of adipose-derived stem cells Stem Cell Res Ther, 2018;9(1):53. 2018 [PMID: 29514703] (IHC, Human) IHC Human
MH Amer, FRAJ Rose, KM Shakesheff, LJ White A biomaterials approach to influence stem cell fate in injectable cell-based therapies Stem Cell Res Ther, 2018;9(1):39. 2018 [PMID: 29467014] (ICC, Human) ICC Human
C Arianna, C Eliana, A Flavio, R Marco, D Giacomo, S Manuel, B Elena, G Alessandra Rapid Rapamycin-Only Induced Osteogenic Differentiation of Blood-Derived Stem Cells and Their Adhesion to Natural and Artificial Scaffolds Stem Cells Int, 2017;2017(0):2976541. 2017 [PMID: 28814956] (ICC, Human) ICC Human
G Lisignoli, E Lambertini, C Manferdini, E Gabusi, L Penolazzi, F Paolella, M Angelozzi, V Casagranda, R Piva Collagen type XV and the 'osteogenic status' J. Cell. Mol. Med, 2017;0(0):. 2017 [PMID: 28332281] (Flow Cytometry, Human) Flow Cytometry Human
Show All 24 Publications.

Reviews for Osteocalcin Antibody (MAB1419) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using MAB1419:
Filter by Applications
ICC
(1)
Flow
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
ICC Rat 02/10/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22364878
SpeciesRat

Blocking

Blocking DetailsSee PMID 22364878

Details

Detection NotesSee PMID 22364878

Comments

CommentsIndependent review from antibodyresource.com
  4
reviewed by:
Verified Customer
Flow Human 02/10/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 22034088
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22034088

Details

Detection NotesSee PMID 22034088

Comments

CommentsIndependent review from antibodyresource.com

FAQs for Osteocalcin Antibody (MAB1419) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC1419U
Alexa Fluor 405 IC1419V
Alexa Fluor 488 IC1419G
Alexa Fluor 532 FAB1419X
Alexa Fluor 594 IC1419T
Alexa Fluor 647 IC1419R
Alexa Fluor 700 IC1419N
Alexa Fluor 750 IC1419S
Allophycocyanin IC1419A
Biotin FAB1419B
DyLight 350 FAB1419D
DyLight 405 FAB1419E
DyLight 488 FAB1419K
DyLight 550 FAB1419L
DyLight 594 FAB1419M
DyLight 650 FAB1419W
DyLight 680 FAB1419Y
DyLight 755 FAB1419Z
FITC FAB1419F
HRP FAB1419H
Janelia Fluor 549 FAB1419I
Janelia Fluor 646 FAB1419J
PerCP FAB1419C
Phycoerythrin IC1419P

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Verified Customer
02/10/2015
Application: ICC
Species: Rat

 
Verified Customer
02/10/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol BGLAP
Uniprot