Recombinant Human PDGF-BB Protein, CF

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1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.
Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human PDGF-BB Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
The specific activity of Recombinant Human PDGF-BB is approximately 1.9 x 106 IU/mg, which is calibrated against recombinant human PDGF-BB WHO International Standard (NIBSC code: 94/728).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human PDGF-BB protein
Ser82-Thr190
Accession #
N-terminal Sequence
Ser82
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
PDGFB
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 4
using
220-BB in the following application:

Publications
Read Publications using
220-BB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF-BB Protein, CF

  • PDGFBB
  • PDGF-BB

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta . Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

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Publications for PDGF-BB (220-BB)(149)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Avian, Canine, N/A, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Rabbit, Synthetic.

We have publications tested in 8 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), ELISA Standard, In Vivo, Surface Plasmon Resonance, novel - monodisperse double em.


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Binding Assay
(1)
Bioassay
(123)
Cell Culture
(13)
ELISA (Standard)
(1)
ELISA Standard
(1)
In Vivo
(3)
Surface Plasmon Resonance
(1)
novel - monodisperse double em
(1)
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Human
(107)
Mouse
(17)
Rat
(5)
Avian
(1)
Canine
(2)
N/A
(2)
Porcine
(1)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Rabbit
(7)
Synthetic
(1)
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Showing Publications 1 - 10 of 149. Show All 149 Publications.
Publications using 220-BB Applications Species
R Boi, K Ebefors, M Henricsson, J Borén, J Nyström Modified lipid metabolism and cytosolic phospholipase A2 activation in mesangial cells under pro-inflammatory conditions Scientific Reports, 2022;12(1):7322. 2022 [PMID: 35513427] (Cell Culture, Human) Cell Culture Human
Kim E, Cha Y, Jeong S, Chang Y Overexpression of CEACAM6 activates Src-FAK signaling and inhibits anoikis, through homophilic interactions in lung adenocarcinomas. Transl Oncol, 0;20(0):101402. 0 [PMID: 35358791] (Bioassay, Human) Bioassay Human
A Joannes, C Morzadec, M Duclos, FL Gutierrez, DC Chiforeanu, C Le Naoures, B De Latour, S Rouzé, L Wollin, S Jouneau, L Vernhet Arsenic trioxide inhibits the functions of lung fibroblasts derived from patients with idiopathic pulmonary fibrosis Toxicology and Applied Pharmacology, 2022;441(0):115972. 2022 [PMID: 35276128] (Cell Culture, Human) Cell Culture Human
SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis International Journal of Molecular Sciences, 2022;23(2):. 2022 [PMID: 35055054] (Bioassay, Human) Bioassay Human
M Culenova, A Nicodemou, ZV Novakova, M Debreova, V Smolinská, S Bernatova, D Ivanisova, O Novotna, J Vasicek, I Varga, S Ziaran, L Danisovic Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells International Journal of Molecular Sciences, 2021;22(22):. 2021 [PMID: 34830384] (Bioassay, Human) Bioassay Human
Y Li, J Shao, J Song, S Yu, J Wang, K Sun MiR-34a-3p suppresses pulmonary vascular proliferation in acute pulmonary embolism rat by targeting DUSP1 Bioscience Reports, 2021;0(0):. 2021 [PMID: 34778900] (Bioassay, Human) Bioassay Human
S Looprasert, A Sereemaspu, N Kitkumthor, K Sooklert, T Sarachana, D Jindatip Gold Nanoparticles Affect Pericyte Biology and Capillary Tube Formation Pharmaceutics, 2021;13(5):. 2021 [PMID: 34067883] (Bioassay, Human) Bioassay Human
S Looprasert, A Sereemaspu, N Kitkumthor, K Sooklert, T Sarachana, D Jindatip Gold Nanoparticles Affect Pericyte Biology and Capillary Tube Formation Pharmaceutics, 2021;13(5):. 2021 [PMID: 34067883] (Bioassay, Human) Bioassay Human
YJ Liao, YH Wang, CY Wu, FY Hsu, CY Chien, YC Lee Ketogenic Diet Enhances the Cholesterol Accumulation in Liver and Augments the Severity of CCl4 and TAA-Induced Liver Fibrosis in Mice International Journal of Molecular Sciences, 2021;22(6):. 2021 [PMID: 33805788] (Bioassay, Human) Bioassay Human
S Yang, H Li, H Yao, Y Zhang, H Bao, L Wu, C Zhang, M Li, L Feng, J Zhang, Z Zheng, G Xu, F Wang Long noncoding RNA ERLR mediates epithelial-mesenchymal transition of retinal pigment epithelial cells and promotes experimental proliferative vitreoretinopathy Cell Death and Differentiation, 2021;0(0):. 2021 [PMID: 33664479] (Bioassay, Rabbit) Bioassay Rabbit
Show All 149 Publications.

Reviews for PDGF-BB (220-BB) (2) 42

Average Rating: 4
(Based on 2 reviews)

Reviews using 220-BB:
Filter by Applications
Immunoassay Standard
(1)
Stem/Immune cell maintenance or differentiation
(1)
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Images Ratings Applications Species Date Details
Immunoassay Standard PDGF-BB 220-BB
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4
reviewed by:
Verified Customer
Immunoassay Standard 10/13/2020
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Summary

ApplicationImmunoassay Standard
LotRecombinant Human PDGF-BB Protein, CF, 10 UG QXB0815111
CommentsGood (publishable, would use again)

Comments

CommentsHMW high intact molecular weight LMW Super low molecular weight
Stem/Immune cell maintenance or differentiation  PDGF-BB 220-BB
Enlarge
4
reviewed by:
Verified Customer
Stem/Immune cell maintenance or differentiation 10/13/2020
View

Summary

ApplicationStem/Immune cell maintenance or differentiation
LotN/A
Comments Good (publishable, would use again)

FAQs for PDGF-BB (220-BB) (0)

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Verified Customer
10/13/2020
Application: Immunoassay Standard
Species:

Verified Customer
10/13/2020
Application: Stem/Immune cell maintenance or differentiation
Species:

Bioinformatics

Gene Symbol PDGFB
Uniprot