Recombinant Human PDGF-BB Protein, CF

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1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.
Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human PDGF-BB Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
The specific activity of Recombinant Human PDGF-BB is approximately 5.8 x 103 IU/μg, which is calibrated against recombinant human PDGF-BB WHO International Standard (NIBSC code: 94/728).
Source
E. coli-derived human PDGF-BB protein
Ser82-Thr190
Accession #
N-terminal Sequence
Ser82
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
PDGFB
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
Publications
Read Publications using
220-BB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF-BB Protein, CF

  • PDGFBB
  • PDGF-BB

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

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Publications for PDGF-BB (220-BB)(116)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Avian, Canine, N/A, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Rabbit.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance.


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Binding Assay
(1)
Bioassay
(102)
Cell Culture
(4)
ELISA (Standard)
(1)
In Vivo
(3)
Surface Plasmon Resonance
(1)
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Human
(79)
Mouse
(16)
Rat
(4)
Avian
(1)
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N/A
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Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Rabbit
(6)
All Species
Showing Publications 1 - 10 of 116. Show All 116 Publications.
Publications using 220-BB Applications Species
L Messerschm, S Fischer, P Wiedemann, A Bringmann, M Hollborn Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation Mol. Vis., 2019;25(0):329-344. 2019 [PMID: 31341381] (Bioassay, Human) Bioassay Human
T Muraguchi, D Nanba, EK Nishimura, T Tashiro IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 31122679] (Bioassay, Human) Bioassay Human
T Emi, K Michaud, E Orton, G Santilli, C Linh, M O'Connell, F Issa, S Kennedy Ultrasonic Generation of Pulsatile and Sequential Therapeutic Delivery Profiles from Calcium-Crosslinked Alginate Hydrogels Molecules, 2019;24(6):. 2019 [PMID: 30884862] (Bioassay) Bioassay
T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair Cell Rep, 2019;26(10):2566-2579.e10. 2019 [PMID: 30840882] (Bioassay, Human) Bioassay Human
MFM Gerli, LA Moyle, S Benedetti, G Ferrari, E Ucuncu, M Ragazzi, C Constantin, I Louca, H Sakai, P Ala, P De Coppi, S Tajbakhsh, G Cossu, FS Tedesco Combined Notch and PDGF Signaling Enhances Migration and Expression of Stem Cell Markers while Inducing Perivascular Cell Features in Muscle Satellite Cells Stem Cell Reports, 2019;0(0):. 2019 [PMID: 30745033] (Bioassay, Human) Bioassay Human
Y Higashi, S Mummidi, S Sukhanov, T Yoshida, M Noda, P Delafontai, B Chandrasek Minocycline inhibits PDGF-BB-induced human aortic smooth muscle cell proliferation and migration by reversing miR-221- and -222-mediated RECK suppression Cell. Signal., 2019;57(0):20-Oct. 2019 [PMID: 30716386] (Bioassay, Human) Bioassay Human
SI Motegi, C Fujiwara, A Sekiguchi, S Yamazaki, Y Yokoyama, M Yasuda, O Ishikawa Possible contribution of PDGF-BB-induced autophagy in dermatofibrosarcoma protuberans: Autophagy marker Atg5 could be a differential marker between dermatofibrosarcoma protuberans and dermatofibroma J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 30704939] (Bioassay, Human) Bioassay Human
H Li, J Zhao, B Liu, J Luo, Z Li, X Qin, Y Wei MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation Int J Med Sci, 2019;16(1):106-114. 2019 [PMID: 30662334] (Bioassay, Human) Bioassay Human
Y Kano, T Gebregiwor, CB Marshall, N Radulovich, BPK Poon, J St-Germain, JD Cook, I Valencia-S, BMM Grant, SG Herrera, J Miao, B Raught, MS Irwin, JE Lee, JJ Yeh, ZY Zhang, MS Tsao, M Ikura, M Ohh Tyrosyl phosphorylation of KRAS stalls GTPase cycle via alteration of switch I and II conformation Nat Commun, 2019;10(1):224. 2019 [PMID: 30644389] (Bioassay, Mouse) Bioassay Mouse
M Zhang, G Urabe, C Little, B Wang, AM Kent, Y Huang, KC Kent, LW Guo HDAC6 Regulates the MRTF-A/SRF Axis and Vascular Smooth Muscle Cell Plasticity JACC Basic Transl Sci, 2018;3(6):782-795. 2018 [PMID: 30623138] (Bioassay, Mouse) Bioassay Mouse
Show All 116 Publications.

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Bioinformatics

Gene Symbol PDGFB
Uniprot