Recombinant Human PDGF-BB Protein, CF

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Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.
1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human PDGF-BB Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
Source
E. coli-derived human PDGF-BB protein
Ser82-Thr190
Accession #
N-terminal Sequence
Ser82
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
PDGFB
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 4
using
220-BB in the following application:

Publications
Read Publications using
220-BB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF-BB Protein, CF

  • c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV
  • PDGFBB
  • PDGF-BB

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta . Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

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Publications for PDGF-BB (220-BB)(169)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Avian, Canine, N/A, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Chlorocebus pygerythrus (Vervet Monkey), Rabbit.

We have publications tested in 8 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), ELISA Standard, In Vivo, Migration Bioassay, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(1)
Bioassay
(148)
Cell Culture
(13)
ELISA (Standard)
(1)
ELISA Standard
(1)
In Vivo
(3)
Migration Bioassay
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(125)
Mouse
(24)
Rat
(9)
Avian
(1)
Canine
(2)
N/A
(2)
Porcine
(2)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Rabbit
(7)
All Species
Showing Publications 1 - 10 of 169. Show All 169 Publications.
Publications using 220-BB Applications Species
Li, X;Quan, H;He, J;Li, H;Zhu, Q;Wang, Y;Zhu, Y;Ge, RS; The role of platelet-derived growth factor BB signaling pathway in the regulation of stem and progenitor Leydig cell proliferation and steroidogenesis in male rats The Journal of steroid biochemistry and molecular biology 2023-06-05 [PMID: 37286111] (Bioassay, Rat) Bioassay Rat
Xu, Y;Zhang, H;Pober, J;Min, W;Zhou, H; SRF SUMOylation modulates smooth muscle phenotypic switch and vascular remodeling Research square 2023-05-22 [PMID: 37292911] (Bioassay, Mouse) Bioassay Mouse
HM Yang, JE Lee, JY Kim, J You, J Kim, HS Lee, HM Yoo, MG Kong, JK Han, HJ Cho, KW Park, HJ Kang, BK Koo, YB Park, HS Kim Identification of cell-biologic mechanisms of coronary artery spasm and its ex vivo diagnosis using peripheral blood-derived iPSCs Biomaterials research, 2023-02-18;27(1):16. 2023-02-18 [PMID: 36803875] (Bioassay, Human) Bioassay Human
S Wenhua, T Tsunematsu, M Umeda, H Tawara, N Fujiwara, Y Mouri, R Arakaki, N Ishimaru, Y Kudo Cancer cell-derived novel periostin isoform promotes invasion in head and neck squamous cell carcinoma Cancer Medicine, 2023-01-23;0(0):. 2023-01-23 [PMID: 36691359] (Bioassay, Human) Bioassay Human
X Cai, Y Mao, X Shen, H Li, J He, M Zhang The extract from Hyssopus cuspidatus Boriss. Prevents bronchial airway remodeling by inhibiting mouse bronchial wall thickening and hASMC proliferation and migration Journal of ethnopharmacology, 2022-12-14;0(0):116047. 2022-12-14 [PMID: 36528211] (Bioassay, Human) Bioassay Human
AA Widjaja, S Viswanatha, SG Shekeran, E Adami, WW Lim, S Chothani, J Tan, JWT Goh, HM Chen, SY Lim, CM Boustany-K, J Hawkins, E Petretto, N Hübner, S Schafer, TM Coffman, SA Cook Targeting endogenous kidney regeneration using anti-IL11 therapy in acute and chronic models of kidney disease Nature Communications, 2022-12-05;13(1):7497. 2022-12-05 [PMID: 36470928] (Bioassay, Human) Bioassay Human
A Dos Santos, N Lyu, A Balayan, R Knight, KS Zhuo, Y Sun, J Xu, ML Funderburg, JL Funderburg, SX Deng Generation of Functional Immortalized Human Corneal Stromal Stem Cells International Journal of Molecular Sciences, 2022-11-02;23(21):. 2022-11-02 [PMID: 36362184] (Bioassay, Human) Bioassay Human
S Gc, K Tuy, L Rickenback, R Jones, A Chakrabort, CR Miller, EA Beierle, VS Hanumanthu, AN Tran, JA Mobley, SL Bellis, AB Hjelmeland alpha2,6 Sialylation mediated by ST6GAL1 promotes glioblastoma growth JCI Insight, 2022-11-08;7(21):. 2022-11-08 [PMID: 36345944] (Bioassay, Human) Bioassay Human
W He, X Zhu, A Xin, H Zhang, Y Sun, H Xu, H Li, T Yang, D Zhou, H Yan, X Sun Long-term maintenance of human endometrial epithelial stem cells and their therapeutic effects on intrauterine adhesion Cell & bioscience, 2022-10-18;12(1):175. 2022-10-18 [PMID: 36258228] (Bioassay, Human) Bioassay Human
Show All 169 Publications.

Reviews for PDGF-BB (220-BB) (2) 42

Average Rating: 4
(Based on 2 reviews)

Reviews using 220-BB:
Filter by Applications
Immunoassay Standard
(1)
Stem/Immune cell maintenance or differentiation
(1)
All Applications
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Images Ratings Applications Species Date Details
Immunoassay Standard PDGF-BB 220-BB
Enlarge
4
reviewed by:
Steven Moore
Immunoassay Standard 10/13/2020
View

Summary

ApplicationImmunoassay Standard
LotRecombinant Human PDGF-BB Protein, CF, 10 UG QXB0815111
CommentsGood (publishable, would use again)

Comments

CommentsHMW high intact molecular weight LMW Super low molecular weight
Stem/Immune cell maintenance or differentiation  PDGF-BB 220-BB
Enlarge
4
reviewed by:
Steven Moore
Stem/Immune cell maintenance or differentiation 10/13/2020
View

Summary

ApplicationStem/Immune cell maintenance or differentiation
LotN/A
Comments Good (publishable, would use again)

FAQs for PDGF-BB (220-BB) (0)

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Recent Reviews

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Steven Moore
10/13/2020
Application: Immunoassay Standard
Species:

Steven Moore
10/13/2020
Application: Stem/Immune cell maintenance or differentiation
Species:

Bioinformatics

Gene Symbol PDGFB
Uniprot