Recombinant Human PDGF-BB Protein, CF

Images

 
1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.
Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations

Recombinant Human PDGF-BB Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
The specific activity of Recombinant Human PDGF-BB is approximately 5.8 x 103 IU/μg, which is calibrated against recombinant human PDGF-BB WHO International Standard (NIBSC code: 94/728).
Source
E. coli-derived human PDGF-BB protein
Ser82-Thr190
Accession #
N-terminal Sequence
Ser82
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
PDGFB
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 4
using
220-BB in the following application:

Publications
Read Publications using
220-BB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF-BB Protein, CF

  • PDGFBB
  • PDGF-BB

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Customers Who Viewed This Item Also Viewed...

NB600-408
Species: Hu, Mu, Rt, Po, Ba, Bv, Fe, Rb
Applications: WB, ELISA, FLISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr
NBP1-58279
Species: Hu
Applications: WB, IHC, IHC-P
NB600-335
Species: All-NA
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-50037
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, KO
NBP2-50599
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
NBP3-05039
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF4928
Species: Hu
Applications: WB, Flow, CyTOF-ready
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
220-BB
Species: Hu
Applications: Bioactivity

Publications for PDGF-BB (220-BB)(124)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Avian, Canine, N/A, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Rabbit, Synthetic.

We have publications tested in 7 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance, novel - monodisperse double em.


Filter By Application
Binding Assay
(1)
Bioassay
(106)
Cell Culture
(7)
ELISA (Standard)
(1)
In Vivo
(3)
Surface Plasmon Resonance
(1)
novel - monodisperse double em
(1)
All Applications
Filter By Species
Human
(86)
Mouse
(16)
Rat
(4)
Avian
(1)
Canine
(3)
N/A
(1)
Porcine
(1)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Rabbit
(6)
Synthetic
(1)
All Species
Showing Publications 1 - 10 of 124. Show All 124 Publications.
Publications using 220-BB Applications Species
X Li, S Shao, H Li, Z Bi, S Zhang, Y Wei, J Bai, R Zhang, X Ma, B Ma, L Zhang, C Xie, W Ning, H Zhou, C Yang Byakangelicin protects against carbon tetrachloride-induced liver injury and fibrosis in mice J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32643868] (Cell Culture, Human) Cell Culture Human
M Oka, S Shimo, N Ohno, H Imai, Y Abekura, H Koseki, H Miyata, K Shimizu, M Kushamae, I Ono, K Nozaki, A Kawashima, T Kawamata, T Aoki Dedifferentiation of smooth muscle cells in intracranial aneurysms and its potential contribution to the pathogenesis Sci Rep, 2020;10(1):8330. 2020 [PMID: 32433495] (Bioassay, Human) Bioassay Human
M Aliabouzar, A Jivani, X Lu, OD Kripfgans, JB Fowlkes, ML Fabiilli Standing wave-assisted acoustic droplet vaporization for single and dual payload release in acoustically-responsive scaffolds Ultrason Sonochem, 2020;66(0):105109. 2020 [PMID: 32248042] (novel - monodisperse double em, Synthetic) novel - monodisperse double em Synthetic
B Wang, M Zhang, G Urabe, Y Huang, G Chen, D Wheeler, DJ Dornbos, A Huttinger, SM Nimjee, S Gong, LW Guo, KC Kent PERK Inhibition Mitigates Restenosis and�Thrombosis: A Potential Low-Thrombogenic Antirestenotic Paradigm JACC Basic Transl Sci, 2020;5(3):245-263. 2020 [PMID: 32215348] (Cell Culture, Human) Cell Culture Human
NA Agabalyan, HD Sparks, S Tarraf, NL Rosin, K Anker, G Yoon, LN Burnett, D Nickerson, ES Di Martino, VA Gabriel, J Biernaskie Adult Human Dermal Progenitor Cell Transplantation Modulates the Functional Outcome of Split-Thickness Skin Xenografts Stem Cell Reports, 2019;13(6):1068-1082. 2019 [PMID: 31735655] (Cell Culture, Human) Cell Culture Human
K Wang, J Yu, B Wang, H Wang, Z Shi, G Li miR-29a Regulates the Proliferation and Migration of Human Arterial Smooth Muscle Cells in Arteriosclerosis Obliterans of the Lower Extremities Kidney Blood Press. Res., 2019;44(5):1219-1232. 2019 [PMID: 31614351] (Bioassay, Human) Bioassay Human
TN Vu, X Chen, HD Foda, GC Smaldone, NA Hasaneen Interferon-γ enhances the antifibrotic effects of pirfenidone by attenuating IPF lung fibroblast activation and differentiation Respir. Res., 2019;20(1):206. 2019 [PMID: 31511015] (Bioassay, Human) Bioassay Human
L Messerschm, S Fischer, P Wiedemann, A Bringmann, M Hollborn Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation Mol. Vis., 2019;25(0):329-344. 2019 [PMID: 31341381] (Bioassay, Human) Bioassay Human
P Luan, W Jian, X Xu, W Kou, Q Yu, H Hu, D Li, W Wang, MW Feinberg, J Zhuang, Y Xu, W Peng NLRC5 inhibits neointima formation following vascular injury and directly interacts with PPARgamma Nat Commun, 2019;10(1):2882. 2019 [PMID: 31253783] (Bioassay, Human) Bioassay Human
T Muraguchi, D Nanba, EK Nishimura, T Tashiro IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 31122679] (Bioassay, Human) Bioassay Human
Show All 124 Publications.

Reviews for PDGF-BB (220-BB) (2) 42

Average Rating: 4
(Based on 2 reviews)

Reviews using 220-BB:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
 PDGF-BB 220-BB
Enlarge
4
reviewed by:
Steven Moore
10/13/2020
View

Summary

LotRecombinant Human PDGF-BB Protein, CF, 10 UG QXB0815111
CommentsGood (publishable, would use again)

Comments

CommentsHMW high intact molecular weight LMW Super low molecular weight
 PDGF-BB 220-BB
Enlarge
4
reviewed by:
Steven Moore
10/13/2020
View

Summary

LotN/A
Comments Good (publishable, would use again)

FAQs for PDGF-BB (220-BB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Steven Moore
10/13/2020
Application:
Species:

Steven Moore
10/13/2020
Application:
Species:

Bioinformatics

Gene Symbol PDGFB
Uniprot