Recombinant Human TGF-beta 1 (Human Cell-expressed), CF


Recombinant human TGF-beta 1 (7754-BH/CF) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TGF-beta 1 (Human Cell-expressed), CF Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.
Human embryonic kidney cell, HEK293-derived human TGF-beta 1 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
12.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
11 kDa, reducing conditions
Read Publications using
7754-BH/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 1 (Human Cell-expressed), CF

  • DPD1
  • latency-associated peptide
  • TGF beta
  • TGF beta1
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1


TGF‑ beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, ‑2 and ‑3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta  RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press p. 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Publications for TGF-beta 1 (7754-BH/CF)(53)

We have publications tested in 4 confirmed species: Human, Mouse, Complex Species Category, Transgenic Mouse.

We have publications tested in 2 applications: Bioassay, Cell Culture.

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Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using 7754-BH/CF Applications Species
L�tge, M;De Martin, A;Gil-Cruz, C;Perez-Shibayama, C;Stanossek, Y;Onder, L;Cheng, HW;Kurz, L;Cadosch, N;Soneson, C;Robinson, MD;Stoeckli, SJ;Ludewig, B;Pikor, NB; Conserved stromal-immune cell circuits secure B cell homeostasis and function Nature immunology 2023-05-18 [PMID: 37202489] (Bioassay, Human) Bioassay Human
V Klück, G Cab?u, L Mies, F Bukkems, L van Emst, R Bakker, A van Caam, HINT conso, TO Cri?an, LAB Joosten TGF-beta is elevated in hyperuricemic individuals and mediates urate-induced hyperinflammatory phenotype in human mononuclear cells Arthritis Research & Therapy, 2023-02-27;25(1):30. 2023-02-27 [PMID: 36850003] (Bioassay, Human) Bioassay Human
VMD Almanzar, K Shah, JF LaComb, A Mojumdar, HR Patel, J Cheung, M Tang, J Ju, AB Bialkowska 5-FU-miR-15a Inhibits Activation of Pancreatic Stellate Cells by Reducing YAP1 and BCL-2 Levels In Vitro International Journal of Molecular Sciences, 2023-02-16;24(4):. 2023-02-16 [PMID: 36835366] (Bioassay, Human, Transgenic Mouse) Bioassay Human, Transgenic Mouse
WL Yang, W Qiu, T Zhang, K Xu, ZJ Gu, Y Zhou, HJ Xu, ZZ Yang, B Shen, YL Zhao, Q Zhou, Y Yang, W Li, PY Yang, YG Yang Nsun2 coupling with RoRgammat shapes the fate of Th17 cells and promotes colitis Nature Communications, 2023-02-16;14(1):863. 2023-02-16 [PMID: 36792629] (Bioassay, Mouse) Bioassay Mouse
DI Cho, MJ Ahn, HH Cho, M Cho, JH Jun, BG Kang, SY Lim, SJ Yoo, MR Kim, HS Kim, SJ Lee, LT Dat, C Lee, YS Kim, Y Ahn ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation Experimental & Molecular Medicine, 2023-02-13;0(0):. 2023-02-13 [PMID: 36782020] (Bioassay, Human) Bioassay Human
AH Mandarano, TL Harris, BM Creasy, M Wehenkel, M Duggar, BA Wilander, A Mishra, JC Crawford, SA Mullen, KM Williams, M Pillai, AA High, MA McGargill DRAK2 contributes to type 1 diabetes by negatively regulating IL-2 sensitivity to alter regulatory T�cell development Cell Reports, 2023-02-10;42(2):112106. 2023-02-10 [PMID: 36773294] (Bioassay, Human) Bioassay Human
M Migda?, T Arakawa, S Takizawa, M Furuno, H Suzuki, E Arner, CL Winata, B Kaczkowski xcore: an R package for inference of gene expression regulators BMC Bioinformatics, 2023-01-11;24(1):14. 2023-01-11 [PMID: 36631751] (Bioassay, Human) Bioassay Human
J Feng, X Han, Y Yuan, CK Cho, E Jane?ková, T Guo, S Pareek, MS Rahman, B Zheng, J Bi, J Jing, M Zhang, J Xu, TV Ho, Y Chai TGF-beta signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development Elife, 2022-12-21;11(0):. 2022-12-21 [PMID: 36542062] (Cell Culture, Mouse) Cell Culture Mouse
DQ Chen, L Chen, Y Guo, XQ Wu, TT Zhao, HL Zhao, HJ Zhang, MH Yan, GQ Zhang, P Li Poricoic acid A suppresses renal fibroblast activation and interstitial fibrosis in UUO rats via upregulating Sirt3 and promoting beta-catenin K49 deacetylation Acta pharmacologica Sinica, 2022-12-05;0(0):. 2022-12-05 [PMID: 36470978] (Bioassay, Human) Bioassay Human
D Cai, SY Chen Nanoparticle endothelial delivery of PGC-1alpha attenuates hypoxia-induced pulmonary hypertension by attenuating EndoMT-caused vascular wall remodeling Redox Biology, 2022-10-28;58(0):102524. 2022-10-28 [PMID: 36334380] (Bioassay, Human) Bioassay Human
Show All 53 Publications.

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Blogs on TGF-beta 1.

TGF-beta 1 - a versatile signaling molecule with roles in development and disease
The transforming growth factor-beta (TGF-beta) family consists of a wide variety of signaling proteins with roles in development. TGF-beta signaling controls growth, differentiation, and immune responses and is often misregulated in cancer. TGF-beta ...  Read full blog post.

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Gene Symbol TGFB1