PTH Antibody (918462) [Unconjugated]

Images

 
Western blot shows lysates of human parathyroid tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more
PTH was detected in immersion fixed paraffin-embedded sections of human parathyroid gland using Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) at 5 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
918462
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PTH Antibody (918462) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PTH
Ser32-Gln115
Accession # P01270
Specificity
Detects human PTH in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTH
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTH Antibody (918462) [Unconjugated]

  • Parathormone
  • Parathyrin
  • parathyroid hormone 1
  • parathyroid hormone
  • PTH
  • PTH1

Background

PTH (Parathyroid Hormone) is a critical hormone in the regulation of Ca++ homeostasis (1). The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone. Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively. Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation (1). PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis (1-3). C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure (1-3). PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus (4). PTH secretion is enhanced by low Ca++ concentrations and inhibited by FGF-23 (1, 5). In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium (6). Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production (2, 7). PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells (8-10). TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca++ from bone (1, 8, 9). PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production (11, 12). In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca++ excretion and increases phosphate excretion (1, 2, 9). PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca++ influx in PTH1R-expressing arterial smooth muscle (8, 13).
  1. Potts, J.T. (2005) J. Endocrinol. 187:311.
  2. Usatii, M. et al. (2007) Kidney Int. 72:1330.
  3. Scillitani, A. et al. (2011) J. Clin. Invest. 34:23.
  4. Liu, Z. et al. (2010) PLoS Genet. 6:e1001251.
  5. Ben-Dov, I.Z. et al. (2007) J. Clin. Invest. 117:4003.
  6. Carnevale, V. et al. (2010) Bone 47:626.
  7. Schipani, E. et al. (1993) Endocrinology 132:2157.
  8. Jacome-Galarza, C.E. et al. (2011) J. Bone Miner. Res. 26:1207.
  9. Lupp, A. et al. (2010) Eur. J. Endocrinol. 162:979.
  10. Fortunati, D. et al. (2010) Matrix Biol. 29:594.
  11. O’Brien, C.A. et al. (2008) PLoS ONE 3:e2942.
  12. Lombardi, G. et al. (2010) J. Endocrinol. Invest. 33:22.
  13. Zaruba, M.M. et al. (2008) Cardiovasc. Res. 77:722.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PTH
Uniprot