Recombinant Human IGF-I/IGF-1 Protein, CF


1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I/IGF-1.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.

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Recombinant Human IGF-I/IGF-1 Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.3-1.5 ng/mL.
The specific activity of recombinant human IGF-I/IGF-1 is approximately 2.5 IU/μg, which is calibrated against recombinant human IGF-I/IGF-1 WHO International Standard (NIBSC code: 91/554).   Specific activity is for reference purposes only and is not routinely tested.  
E. coli-derived human IGF-I/IGF-1 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
7 kDa, reducing conditions
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291-G1 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-I/IGF-1 Protein, CF

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C


Insulin-like growth factor I (IGF-I or IGF-1), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C‑terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-1 protein is identical between isoforms and is generated by proteolytic removal of the N- and C‑terminal regions. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II/IGF-2. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4‑7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). The IGF-I protein associates with IGF binding proteins thereby increasing its plasma half‑life and modulating its interactions with receptors (10).

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

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Publications for IGF-I/IGF-1 (291-G1)(173)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Feline, Hamster, Human`, Insect, L. braziliensis, N/A, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit.

We have publications tested in 9 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), In Vivo, Neutralization, Scaffold Fabrication, Surface Plasmon Resonance, Western Blot.

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L. braziliensis
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
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Showing Publications 1 - 10 of 173. Show All 173 Publications.
Publications using 291-G1 Applications Species
SL Pereira, ME Shoemaker, S Gawel, GJ Davis, M Luo, VA Mustad, JT Cramer Biomarker Changes in Response to a 12-Week Supplementation of an Oral Nutritional Supplement Enriched with Protein, Vitamin D and HMB in Malnourished Community Dwelling Older Adults with Sarcopenia Nutrients, 2022;14(6):. 2022 [PMID: 35334853] (Bioassay, Human) Bioassay Human
G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL Stem Cell Research, 2021;59(0):102635. 2021 [PMID: 35074682] (Bioassay, Human) Bioassay Human
AF de Souza, FF Bressan, NCG Pieri, RC Botigelli, T Revay, SK Haddad, DT Covas, ES Ramos, WA King, FV Meirelles Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients Cells, 2021;10(11):. 2021 [PMID: 34831322] (Bioassay, Human) Bioassay Human
KD Abu-Bonsra, DF Newgreen, M Dottori Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds Cells, 2021;10(11):. 2021 [PMID: 34831120] (Bioassay, Human) Bioassay Human
AN Holowatyj, B Gigic, CA Warby, J Ose, T Lin, P Schrotz-Ki, CM Ulrich, JJ Bernard The Use of Human Serum Samples to Study Malignant Transformation: A Pilot Study Cells, 2021;10(10):. 2021 [PMID: 34685650] (Bioassay, Human) Bioassay Human
W M Kholosy, M Derieppe, F van den Ha, K Ober, Y Su, L Custers, L Schild, L M J van Zo, L M Wellens, H R Ariese, CL Szanto, J Wienke, MP Dierselhui, D van Vuurde, EM Dolman, JJ Molenaar Neuroblastoma and DIPG Organoid Coculture System for Personalized Assessment of Novel Anticancer Immunotherapies Journal of personalized medicine, 2021;11(9):. 2021 [PMID: 34575646] (Bioassay, Human) Bioassay Human
PR Gajjala, RK Kasam, D Soundarara, D Sinner, SK Huang, AG Jegga, SK Madala Dysregulated overexpression of Sox9 induces fibroblast activation in pulmonary fibrosis JCI Insight, 2021;0(0):. 2021 [PMID: 34520400] (Bioassay, Human) Bioassay Human
AM Thiebaut, I Buendia, V Ginet, E Lemarchand, MB Boudjadja, Y Hommet, L Lebouvier, C Lechevalli, M Maillasson, E Hedou, N Déglon, F Oury, M Rubio, J Montaner, J Puyal, D Vivien, BD Roussel Thrombolysis by PLAT/tPA increases serum free IGF1 leading to a decrease of deleterious autophagy following brain ischemia Autophagy, 2021;0(0):1-21. 2021 [PMID: 34520334] (Bioassay, Human) Bioassay Human
G Iannello, A Patel, D Sirabella, B Corneo, V Thaker Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity Stem Cell Research, 2021;54(0):102432. 2021 [PMID: 34214899] (Bioassay, Human) Bioassay Human
KMA Abdel-Raou, R Rezgui, C Stefanini, JCM Teo, N Christofor Transdifferentiation of Human Fibroblasts into Skeletal Muscle Cells: Optimization and Assembly into Engineered Tissue Constructs through Biological Ligands Biology, 2021;10(6):. 2021 [PMID: 34208436] (Cell Culture, Human) Cell Culture Human
Show All 173 Publications.

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Gene Symbol IGF1