Recombinant Human IGF-I/IGF-1 Protein, CF


1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I/IGF-1.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.

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Applications Bioactivity

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Recombinant Human IGF-I/IGF-1 Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.3-1.5 ng/mL.
The specific activity of recombinant human IGF-I/IGF-1 is approximately 2.5 IU/μg, which is calibrated against recombinant human IGF-I/IGF-1 WHO International Standard (NIBSC code: 91/554).
E. coli-derived human IGF-I/IGF-1 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
7 kDa, reducing conditions
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291-G1 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-I/IGF-1 Protein, CF

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C


Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I/IGF-1 is synthesized as two precursor isoforms with N- and alternate C‑terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C‑terminal regions. Mature human IGF-I/IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4‑7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half‑life and modulates its interactions with receptors (10).

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

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Publications for IGF-I/IGF-1 (291-G1)(137)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Insect, N/A, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit.

We have publications tested in 8 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), In Vivo, Neutralization, Scaffold Fabrication, Western Blot.

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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
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Showing Publications 1 - 10 of 137. Show All 137 Publications.
Publications using 291-G1 Applications Species
K Kawai, R Negoro, M Ichikawa, T Yamashita, S Deguchi, K Harada, K Hirata, K Takayama, H Mizuguchi Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies Mol Ther Methods Clin Dev, 2020;17(0):49-57. 2020 [PMID: 31890740] (Cell Culture, Human) Cell Culture Human
M Matjusaiti, LJ Wagstaff, A Martella, B Baranowski, C Blin, S Gogolok, A Williams, SM Pollard Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors Stem Cell Reports, 2019;13(6):1053-1067. 2019 [PMID: 31708478] (Cell Culture, Mouse) Cell Culture Mouse
S Krueger, S Achilles, J Zimmermann, T Tischer, R Bader, A Jonitz-Hei Re-Differentiation Capacity of Human Chondrocytes in Vitro Following Electrical Stimulation with Capacitively Coupled Fields J Clin Med, 2019;8(11):. 2019 [PMID: 31652962] (Bioassay, Human) Bioassay Human
A Tripathi, C Volsko, JP Garcia, E Agirre, KC Allan, PJ Tesar, BD Trapp, G Castelo-Br, FJ Sim, R Dutta Oligodendrocyte Intrinsic miR-27a Controls Myelination and Remyelination Cell Rep, 2019;29(4):904-919.e9. 2019 [PMID: 31644912] (Bioassay, Mouse) Bioassay Mouse
JC Butts, N Iyer, N White, R Thompson, S Sakiyama-E, TC McDevitt V2a interneuron differentiation from mouse and human pluripotent stem cells Nat Protoc, 2019;0(0):. 2019 [PMID: 31628445] (Bioassay, Human) Bioassay Human
T Feng, AA DeVore, MC Perego, BC Morrell, LJ Spicer Effects of N-carbamylglutamate and arginine on steroidogenesis and proliferation of pig granulosa cells in vitro Anim. Reprod. Sci., 2019;209(0):106138. 2019 [PMID: 31514935] (Bioassay, Porcine) Bioassay Porcine
Z Wang, G Liu, J Mao, M Xie, M Zhao, X Guo, S Liang, H Li, X Li, R Wang IGF-1R Inhibition Suppresses Cell Proliferation and Increases Radiosensitivity in Nasopharyngeal Carcinoma Cells Mediators Inflamm., 2019;2019(0):5497467. 2019 [PMID: 31467485] (Bioassay, Human) Bioassay Human
YB Pang, J He, BY Cui, S Xu, XL Li, MY Wu, R Liang, Y Feng, X Guo, XH Zhang, XL Luo A Potential Antitumor Effect of Dendritic Cells Fused with Cancer Stem Cells in Hepatocellular Carcinoma Stem Cells Int, 2019;2019(0):5680327. 2019 [PMID: 31065274] (Bioassay, Human) Bioassay Human
S Mayers, PD Moço, T Maqbool, PN Silva, DM Kilkenny, J Audet Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector BMC Biotechnol., 2019;19(1):21. 2019 [PMID: 30987611] (Bioassay, Mouse) Bioassay Mouse
B Mair, J Tomic, SN Masud, P Tonge, A Weiss, M Usaj, AHY Tong, JJ Kwan, KR Brown, E Titus, M Atkins, KSK Chan, L Munsie, A Habsid, H Han, M Kennedy, B Cohen, G Keller, J Moffat Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies Cell Rep, 2019;27(2):599-615.e12. 2019 [PMID: 30970261] (Differentiation, Human) Differentiation Human
Show All 137 Publications.

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Gene Symbol IGF1