Recombinant Human IGF-I/IGF-1 Protein, CF

1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.

Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.

MALDI-TOF analysis of Recombinant Human IGF-I/IGF-1.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IGF-I/IGF-1 Protein, CF Summary

• Details of Functionality: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 0.3-1.5 ng/mL.
  The specific activity of recombinant human IGF-I/IGF-1 is approximately 2.5 IU/μg, which is calibrated against recombinant human IGF-I/IGF-1 WHO International Standard (NIBSC code: 91/554).
• Source: E. coli-derived human IGF-I/IGF-1 protein
  Gly49-Ala118
• Accession #: P05019
• N-terminal Sequence: Gly49
• Protein/Peptide Type: Recombinant Proteins
• Gene: IGF1
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 7.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 7 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-I/IGF-1 Protein, CF

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I/IGF-1 is synthesized as two precursor isoforms with N- and alternate C‑terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C‑terminal regions. Mature human IGF-I/IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4‑7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half‑life and modulates its interactions with receptors (10).

1. Philippou, A. et al. (2007) In Vivo 21:45.
2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Publications for IGF-I/IGF-1 (291-G1)(121)

Publications using 291-G1

• CA Conover, LK Bale, RL Frye, HV Schaff Cellular characterization of human epicardial adipose tissue: highly expressed PAPP-A regulates insulin-like growth factor I signaling in human cardiomyocytes Physiol Rep, 2019;7(4):e14006. 2019 [PMID: 30809969] (Bioassay, Human)
• S Ashraf, S Bell, C O'Leary, P Canning, I Micu, JA Fernandez, M O'Hare, P Barabas, H McCauley, DP Brazil, AW Stitt, JG McGeown, TM Curtis CAMKII as a therapeutic target for growth factor-induced retinal and choroidal neovascularisation JCI Insight, 2019;0(0):. 2019 [PMID: 30721154] (Bioassay, Human)
• KA Rankin, F Mei, K Kim, YA Shen, SR Mayoral, C Desponts, DS Lorrain, AJ Green, SE Baranzini, JR Chan, R Bove Selective estrogen receptor modulators enhance CNS remyelination independent of estrogen receptors J. Neurosci., 2019;0(0):. 2019 [PMID: 30696729] (Bioassay, Human)
• LP Foltz, SE Howden, JA Thomson, DO Clegg Functional Assessment of Patient-Derived Retinal Pigment Epithelial Cells Edited by CRISPR/Cas9 Int J Mol Sci, 2018;19(12):. 2018 [PMID: 30572641] (Bioassay, Human)
• J Li, J Zheng Theaflavins prevent cartilage degeneration via AKT/FOXO3 signaling in�vitro Mol Med Rep, 2019;19(2):821-830. 2019 [PMID: 30569095] (Bioassay, Human)
• M Osipovitch, A Asenjo Mar, JN Mariani, A Cornwell, S Dhaliwal, L Zou, D Chandler-M, S Wang, X Li, SJ Benraiss, R Agate, A Lampp, A Benraiss, MS Windrem, SA Goldman Human ESC-Derived Chimeric Mouse Models of Huntington&#039;s Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30554964] (Bioassay, Human)
• R Negoro, K Takayama, K Kawai, K Harada, F Sakurai, K Hirata, H Mizuguchi Efficient Generation of Small Intestinal Epithelial-like Cells from Human iPSCs for Drug Absorption and Metabolism Studies Stem Cell Reports, 2018;0(0):. 2018 [PMID: 30472010] (Bioassay, Human)
• B Decaesteke, G Denecker, C Van Neste, EM Dolman, W Van Loocke, M Gartlgrube, C Nunes, F De Vloed, P Depuydt, K Verboom, D Rombaut, S Loontiens, J De Wyn, WM Kholosy, B Koopmans, AHW Essing, C Herrmann, D Dreidax, K Durinck, D Deforce, F van Nieuwe, A Henssen, R Versteeg, V Boeva, G Schleierma, J van Nes, P Mestdagh, S Vanhauwaer, JH Schulte, F Westermann, JJ Molenaar, K De Preter, F Speleman TBX2 is a neuroblastoma core regulatory circuitry component enhancing MYCN/FOXM1 reactivation of DREAM targets Nat Commun, 2018;9(1):4866. 2018 [PMID: 30451831] (Bioassay, Human)
• TM Totiger, S Srinivasan, VR Jala, P Lamichhane, AR Dosch, AA Gaidarski, C Joshi, S Rangappa, J Castellano, PK Vemula, X Chen, D Kwon, N Kashikar, M VanSaun, NB Merchant, NS Nagathihal Urolithin A, a novel natural compound to target PI3K/AKT/mTOR pathway in pancreatic cancer Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 30404927] (Bioassay, Human)
• IF LaRocque-d, JDB Rocha, MP Nunes, PAV Oliveira, DO Nascimento, L Freire-de-, CM Takiya, A Morrot, D Decote-Ric, JO Previato, GA DosReis, L Mendonça-P, CG Freire-de- Involvement of the capsular GalXM-induced IL-17 cytokine in the control of Cryptococcus neoformans infection Sci Rep, 2018;8(1):16378. 2018 [PMID: 30401972] (Bioassay, Human)
• H Takeuchi, S Inagaki, W Morozumi, Y Nakano, Y Inoue, Y Kuse, T Mizoguchi, S Nakamura, M Funato, H Kaneko, H Hara, M Shimazawa VGF nerve growth factor inducible is involved in retinal ganglion cells death induced by optic nerve crush Sci Rep, 2018;8(1):16443. 2018 [PMID: 30401804] (Bioassay, Human)
• N Brookhouse, P Zhang, R Caselli, JJ Kim, DA Brafman Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant-Alzheimer&#039;s disease (ASUi005-A) and healthy non-demented control (ASUi006-A) Stem Cell Res, 2018;32(0):145-149. 2018 [PMID: 30296667] (Bioassay, Human)
• LC Reis, EM Ramos-Sanc, F Petitto-As, AH Nerland, M Hernandez-, F Selheim, LM Floeter-Wi, H Goto Insulin-Like Growth Factor-I as an Effector Element of the Cytokine IL-4 in the Development of a Leishmania major Infection Mediators Inflamm., 2018;2018(0):9787128. 2018 [PMID: 30150896] (Mouse)
• YY Lin, Y Chien, JH Chuang, CC Chang, YP Yang, YH Lai, WL Lo, KH Chien, TI Huo, CY Wang Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars Int J Mol Sci, 2018;19(9):. 2018 [PMID: 30149618] (Bioassay, Human)
• S Koyuncu, I Saez, HJ Lee, R Gutierrez-, W Pokrzywa, A Fatima, T Hoppe, D Vilchez The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington&#039;s disease patients Nat Commun, 2018;9(1):2886. 2018 [PMID: 30038412] (Bioassay, Human)
• FC Yang, J Draper, PG Smith, JL Vivian, SM Shapiro, JA Stanford Short Term Development and Fate of MGE-Like Neural Progenitor Cells in Jaundiced and Non-Jaundiced Rat Brain Cell Transplant, 2018;0(0):9636897187663. 2018 [PMID: 29845869] (Bioassay, Human)
• MK Shanmugam, KS Ahn, JH Lee, R Kannaiyan, N Mustafa, KA Manu, KS Siveen, G Sethi, WJ Chng, AP Kumar Celastrol Attenuates the Invasion and Migration and Augments the Anticancer Effects of Bortezomib in a Xenograft Mouse Model of Multiple Myeloma Front Pharmacol, 2018;9(0):365. 2018 [PMID: 29773987] (Bioassay, Human)
• S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Bioassay, Human)
• G Zhou, H Jiang, Z Yin, Y Liu, Q Zhang, C Zhang, B Pan, J Zhou, X Zhou, H Sun, D Li, A He, Z Zhang, W Zhang, W Liu, Y Cao In Vitro Regeneration of Patient-specific Ear-shaped Cartilage and Its First Clinical Application for Auricular Reconstruction EBioMedicine, 2018;28(0):287-302. 2018 [PMID: 29396297] (Bioassay, Human)
• L Todd, L Suarez, C Quinn, AJ Fischer Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of M�ller Glia-Derived Progenitor Cells in the Avian Retina Stem Cells, 2017;0(0):. 2017 [PMID: 29193451] (In Vivo, Chicken)
• Jun-Hung Cho, Bhaumik Patel, Santosh Bonala, Sasikanth Manne, Yan Zhou, Surya K Vadrevu, Jalpa Patel, Marco Peronaci, Shanawaz Ghouse, Elizabeth P Henske, Fabrice Roegiers, Krinio Giannikou, David J Kwiatkows, Hossein Mansouri, Maciej M Markiewsk, Brandon White, Magdalena Karbownic Notch transactivates Rheb to maintain the multipotency of TSC-null cells. Nature Communications, 2017;0(0):2041-1723. 2017 [PMID: 29184052] (Bioassay, Human)
• E Varga, C Nemes, I Bock, Z Táncos, S Berzsenyi, G Lévay, V Román, J Kobolák, A Dinnyés Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) Stem Cell Res, 2017;21(0):19-22. 2017 [PMID: 28677532] (Bioassay, Human)
• P Haynes, K Kernan, SL Zhou, DG Miller Expression patterns of FSHD-causing DUX4 and myogenic transcription factors PAX3 and PAX7 are spatially distinct in differentiating human stem cell cultures Skelet Muscle, 2017;7(1):13. 2017 [PMID: 28637492] (Bioassay, Human)
• JR Chao, DA Lamba, TR Klesert, A Torre, A Hoshino, RJ Taylor, A Jayabalu, AL Engel, TH Khuu, RK Wang, M Neitz, J Neitz, TA Reh Transplantation of Human Embryonic Stem Cell-Derived Retinal Cells into the Subretinal Space of a Non-Human Primate Transl Vis Sci Technol, 2017;6(3):4. 2017 [PMID: 28516002] (Bioassay, Human)
• TT Li, D Zhu, T Mou, Z Guo, JL Pu, QS Chen, XF Wei, ZJ Wu IL-37 induces autophagy in hepatocellular carcinoma cells by inhibiting the PI3K/AKT/mTOR pathway Mol. Immunol., 2017;87(0):132-140. 2017 [PMID: 28433890] (Bioassay, Human)
• DJ Tischfield, J Kim, SA Anderson Atypical PKC and Notch Inhibition Differentially Modulate Cortical Interneuron Subclass Fate from Embryonic Stem Cells Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28416285] (Bioassay, Mouse)
• ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes Am. J. Hum. Genet., 2017;100(4):617-634. 2017 [PMID: 28366443] (Bioassay, Human)
• Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, Goldman S, Fossati V, Hobson G, Tesar P Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes. Am J Hum Genet, 0;100(4):617-634. 0 [PMID: 28366443] (Bioassay, Human)
• J Mercado-Ma, JL Clark, AJ Piper, J Janusis, LM Shaw Differential involvement of the microtubule cytoskeleton in Insulin Receptor Substrate-1 (IRS-1) and IRS-2 signaling to AKT determines response to microtubule disruption in breast carcinoma cells J. Biol. Chem, 2017;0(0):. 2017 [PMID: 28320862] (Bioassay, Human)
• H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3 Sci Rep, 2017;7(0):43736. 2017 [PMID: 28252047] (Bioassay, Human)
• T Garrido-Go, K Ona, M Kapidzic, M Gormley, C Simón, O Genbacev, SJ Fisher Severe pre-eclampsia is associated with alterations in cytotrophoblasts of the smooth chorion Development, 2017;144(5):767-777. 2017 [PMID: 28232601] (Bioassay, Human)
• H Kim, SY Moon, MY Sohn, WJ Lee Insulin-Like Growth Factor-1 Increases the Expression of Inflammatory Biomarkers and Sebum Production in Cultured Sebocytes Ann Dermatol, 2017;29(1):20-25. 2017 [PMID: 28223742] (Bioassay, Human)
• J Shao, PY Zhou, GH Peng Experimental Study of the Biological Properties of Human Embryonic Stem Cell-Derived Retinal Progenitor Cells Sci Rep, 2017;7(0):42363. 2017 [PMID: 28205557] (Bioassay, Human)
• C Lorenz, P Lesimple, R Bukowiecki, A Zink, G Inak, B Mlody, M Singh, M Semtner, N Mah, K Aur‚, M Leong, O Zabiegalov, EM Lyras, V Pfiffer, B Fauler, J Eichhorst, B Wiesner, N Huebner, J Priller, T Mielke, D Meierhofer, Z Izsv k, JC Meier, F Bouillaud, J Adjaye, M Schuelke, EE Wanker, A LombŠs, A Prigione Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28132834] (Bioassay, Human)
• NA Afratis, P Bouris, SS Skandalis, HA Multhaupt, JR Couchman, AD Theocharis, NK Karamanos IGF-IR cooperates with ER? to inhibit breast cancer cell aggressiveness by regulating the expression and localisation of ECM molecules Sci Rep, 2017;7(0):40138. 2017 [PMID: 28079144] (Bioassay, Human)
• G Dalton, SW An, SI Al-Juboori, N Nischan, J Yoon, E Dobrinskik, DW Hilgemann, J Xie, K Luby-Phelp, JJ Kohler, L Birnbaumer, CL Huang Soluble klotho binds monosialoganglioside to regulate membrane microdomains and growth factor signaling Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):752-757. 2017 [PMID: 28069944] (BioassayCell, Human)
• L J Spicer G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications Domest. Anim. Endocrinol, 2016;59(0):90-99. 2016 [PMID: 28040605] (Bioassay, Bovine)
• Ivan Carcamo-Or Analysis of Transcriptional Variability in a Large Human iPSC Library Reveals Genetic and Non-genetic Determinants of Heterogeneity Cell Stem Cell, 2016;0(0):. 2016 [PMID: 28017796] (Bioassay, Human)
• Hugh S O'Neill A Collagen Cardiac Patch Incorporating Alginate Microparticles Permits the Controlled Release of HGF and IGF-1 to Enhance Cardiac Stem Cell Migration and Proliferation J Tissue Eng Regen Med, 2016;0(0):. 2016 [PMID: 27943590] (Scaffold Fabrication)
• Maria Chiara Perego Evidence for direct effects of glyphosate on ovarian function: glyphosate influences steroidogenesis and proliferation of bovine granulosa but not theca cells in vitro J Appl Toxicol, 2016;0(0):. 2016 [PMID: 27917511] (Bioassay, Bovine)
• Tong Wu TGF? signaling confers sorafenib resistance via induction of multiple RTKs in hepatocellular carcinoma cells Mol. Carcinog., 2016;0(0):. 2016 [PMID: 27862334] (Bioassay, Human)
• L J Spicer Fibroblast growth factor 9 (FGF9) regulation of cyclin D1 and cyclin-dependent kinase-4 in ovarian granulosa and theca cells of cattle Mol. Cell. Endocrinol., 2016;0(0):. 2016 [PMID: 27816766] (Bioassay, Bovine)
• The tobacco-specific carcinogen-operated calcium channel promotes lung tumorigenesis via IGF2 exocytosis in lung epithelial cells Nat Commun, 2016;7(0):12961. 2016 [PMID: 27666821] (Neut,, Human)
• Polyurethane scaffolds seeded with CD34(+) cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors Sci Rep, 2016;6(0):32149. 2016 [PMID: 27573994] (Bioassay, Human)
• 3D patterned stem cell differentiation using thermo-responsive methylcellulose hydrogel molds Sci Rep, 2016;6(0):29408. 2016 [PMID: 27381562] (Human)
• Matthew N Svalina IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma Sci Rep, 2016;6(0):27012. 2016 [PMID: 27255663] (Bioassay, Human)
• Structural and functional investigations of the effector protein LpiR1 from Legionella pneumophila J Biol Chem, 2016;0(0):. 2016 [PMID: 27226543] (Bioassay, Human)
• Application of single-cell RNA sequencing in optimizing a combinatorial therapeutic strategy in metastatic renal cell carcinoma Genome Biol, 2016;17(1):80. 2016 [PMID: 27139883] (Bioassay, Human)
• L Bieghs, M Brohus, IB Kristensen, N Abildgaard, M Bøgsted, HE Johnsen, CA Conover, E De Bruyne, K Vanderkerk, MT Overgaard, M Nyegaard Abnormal IGF-Binding Protein Profile in the Bone Marrow of Multiple Myeloma Patients PLoS ONE, 2016;11(4):e0154256. 2016 [PMID: 27111220] (Bioassay, Human)
• Peter E Clayton Metabolites involved in glycolysis and amino acid metabolism are altered in short children born small for gestational age Pediatr. Res., 2016;80(2):299-305. 2016 [PMID: 27057740] (Bioassay, Human)
• Yasuhiro Ikeda Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration Stem Cells Transl Med, 2016;5(5):694-702. 2016 [PMID: 26987352] (Bioassay, Human)
• Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration Stem Cells Transl Med, 2016;5(5):694-702. 2016 [PMID: 26987352] (Bioassay, Human)
• Bolomsky A, Hose D, Schreder M, Seckinger A, Lipp S, Klein B, Heintel D, Ludwig H, Zojer N Insulin like growth factor binding protein 7 (IGFBP7) expression is linked to poor prognosis but may protect from bone disease in multiple myeloma. J Hematol Oncol, 2015;8(1):10. 2015 [PMID: 25887188] (Bioassay, Human)
• Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G EphrinB2 controls vessel pruning through STAT1-JNK3 signalling. Nat Commun, 2015;6(0):6576. 2015 [PMID: 25807892] (Bioassay, Human)
• Patterson, Scott D, Rossi, John M, Paweletz, Katherin, Fitzpatrick, V Dan, Begley, C Glenn, Busse, Leigh, Elliott, Steve, McCaffery, Ian Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissues. PLoS ONE, 2015;10(3):e0122149. 2015 [PMID: 25807104] (Bioassay, Human)
• Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells. J Clin Invest, 2015;125(3):1243-54. 2015 [PMID: 25664855] (Bioassay, Primate - Macaca nemestrina (Southern Pig-tailed Macaque))
• Izumikawa T, Sato B, Mikami T, Tamura J, Igarashi M, Kitagawa H GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) is the preferred substrate for chondroitin N-acetylgalactosaminyltransferase-1. J Biol Chem, 2015;290(9):5438-48. 2015 [PMID: 25568321] (cell culture, Human)
• Lee H, Lee J, Lee S, Cho H, Song H, Jeong D, Seo Y, Shin S, Joung J, Kwon Y, Choi Y, Park W, Lee H, Seol H, Shim Y, Joo K, Nam D Patient-derived xenografts from non-small cell lung cancer brain metastases are valuable translational platforms for the development of personalized targeted therapy. Clin Cancer Res, 2015;21(5):1172-82. 2015 [PMID: 25549722] (Bioassay, Human)
• Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells. Reprod Biol Endocrinol, 2015;12(0):123. 2015 [PMID: 25475528] (Bioassay, Human)
• Li H, Xu L, Zhao L, Ma Y, Zhu Z, Liu Y, Qu X Insulin-like growth factor-I induces epithelial to mesenchymal transition via GSK-3beta and ZEB2 in the BGC-823 gastric cancer cell line. Oncol Lett, 2015;9(1):143-148. 2015 [PMID: 25435948] (Bioassay, Human)
• Cho Y, Hur S, Kim J, Kim J, Lee D, Choe J, Won M, Ha K, Jeoung D, Han S, Ryoo S, Lee H, Min J, Kwon Y, Kim D, Kim Y Specific activation of insulin-like growth factor-1 receptor by ginsenoside Rg5promotes angiogenesis and vasorelaxation. J Biol Chem, 2015;290(1):467-77. 2015 [PMID: 25391655] (Bioassay, Human)
• Chinen Y, Kuroda J, Shimura Y, Nagoshi H, Kiyota M, Yamamoto-Sugitani M, Mizutani S, Sakamoto N, Ri M, Kawata E, Kobayashi T, Matsumoto Y, Horiike S, Iida S, Taniwaki M Phosphoinositide protein kinase PDPK1 is a crucial cell signaling mediator in multiple myeloma. Cancer Res, 2014;74(24):7418-29. 2014 [PMID: 25269480] (Bioassay, Human)
• Drexler A, Pietri J, Pakpour N, Hauck E, Wang B, Glennon E, Georgis M, Riehle M, Luckhart S Human IGF1 regulates midgut oxidative stress and epithelial homeostasis to balance lifespan and Plasmodium falciparum resistance in Anopheles stephensi. PLoS Pathog, 2014;10(6):e1004231. 2014 [PMID: 24968248] (In Vivo, Insect)
• Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes. Stem Cell Reports, 2014;2(5):648-61. 2014 [PMID: 24936452] (Bioassay, Human)
• Mimura N, Hideshima T, Shimomura T, Suzuki R, Ohguchi H, Rizq O, Kikuchi S, Yoshida Y, Cottini F, Jakubikova J, Cirstea D, Gorgun G, Minami J, Tai Y, Richardson P, Utsugi T, Iwama A, Anderson K Selective and potent Akt inhibition triggers anti-myeloma activities and enhances fatal endoplasmic reticulum stress induced by proteasome inhibition. Cancer Res, 2014;74(16):4458-69. 2014 [PMID: 24934808] (Bioassay, Human)
• Cubbon R, Yuldasheva N, Viswambharan H, Mercer B, Baliga V, Stephen S, Askham J, Sukumar P, Skromna A, Mughal R, Walker A, Bruns A, Bailey M, Galloway S, Imrie H, Gage M, Rakobowchuk M, Li J, Porter K, Ponnambalam S, Wheatcroft S, Beech D, Kearney M Restoring Akt1 activity in outgrowth endothelial cells from South Asian men rescues vascular reparative potential. Stem Cells, 2014;32(10):2714-23. 2014 [PMID: 24916783] (Bioassay, Human)
• Li H, Xu L, Li C, Zhao L, Ma Y, Zheng H, Li Z, Zhang Y, Wang R, Liu Y, Qu X Ubiquitin ligase Cbl-b represses IGF-I-induced epithelial mesenchymal transition via ZEB2 and microRNA-200c regulation in gastric cancer cells. Mol Cancer, 2014;13(0):136. 2014 [PMID: 24885194] (Bioassay, Human)
• Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells. Br J Cancer, 2014;110(12):2865-73. 2014 [PMID: 24853185] (Bioassay, Human)
• Wang, Lu-Kai, Hsiao, Tzu-Hung, Hong, Tse-Ming, Chen, Hsuan-Yu, Kao, Shih-Han, Wang, Wen-Lung, Yu, Sung-Lia, Lin, Ching-We, Yang, Pan-Chyr MicroRNA-133a suppresses multiple oncogenic membrane receptors and cell invasion in non-small cell lung carcinoma. PLoS ONE, 2014;9(5):e96765. 2014 [PMID: 24816813] (Bioassay, Human)
• Burnight E, Wiley L, Drack A, Braun T, Anfinson K, Kaalberg E, Halder J, Affatigato L, Mullins R, Stone E, Tucker B CEP290 gene transfer rescues Leber congenital amaurosis cellular phenotype. Gene Ther, 2014;21(7):662-72. 2014 [PMID: 24807808] (Bioassay, Human)
• Wang, Lin, Cao, Lan, Shansky, Janet, Wang, Zheng, Mooney, David, Vandenburgh, Herman Minimally invasive approach to the repair of injured skeletal muscle with a shape-memory scaffold. Mol Ther, 2014;22(8):1441-9. 2014 [PMID: 24769909] (In Vivo, Mouse)
• Pei X, Dai Y, Felthousen J, Chen S, Takabatake Y, Zhou L, Youssefian L, Sanderson M, Bodie W, Kramer L, Orlowski R, Grant S Circumvention of Mcl-1-dependent drug resistance by simultaneous Chk1 and MEK1/2 inhibition in human multiple myeloma cells. PLoS ONE, 2014;9(3):e89064. 2014 [PMID: 24594907] (Bioassay, Human)
• Go G, Srivastava R, Hernandez-Ono A, Gang G, Smith S, Booth C, Ginsberg H, Mani A The combined hyperlipidemia caused by impaired Wnt-LRP6 signaling is reversed by Wnt3a rescue. Cell Metab, 2014;19(2):209-20. 2014 [PMID: 24506864] (Bioassay, Mouse)
• Liang Z, Diepstra A, Xu C, van Imhoff G, Plattel W, Van Den Berg A, Visser L Insulin-like growth factor 1 receptor is a prognostic factor in classical Hodgkin lymphoma. PLoS ONE, 2014;9(1):e87474. 2014 [PMID: 24489919] (Bioassay, Human)
• Belzile, Jean-Phi, Stark, Thomas J, Yeo, Gene W, Spector, Deborah Human cytomegalovirus infection of human embryonic stem cell-derived primitive neural stem cells is restricted at several steps but leads to the persistence of viral DNA. J Virol, 2014;88(8):4021-39. 2014 [PMID: 24453373] (Bioassay, Human)
• Shen X, Zhang Y, Feng Y, Zhang L, Li J, Xie Y, Luo X Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway. Int J Oncol, 2014;44(3):791-6. 2014 [PMID: 24402647] (Bioassay, Human)
• Elloul, Sivan, Kedrin, Dmitriy, Knoblauch, Nicholas, Beck, Andrew H, Toker, Alex The adherens junction protein afadin is an AKT substrate that regulates breast cancer cell migration. Mol Cancer Res, 2014;12(3):464-76. 2014 [PMID: 24269953]
• Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, Markmann J, Miyazaki S, Miyazaki J, Szot G, Bottino R, Kim S Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. Elife, 2013;2(0):e00940. 2013 [PMID: 24252877] (Bioassay, Human)
• Hung C, Rohani M, Lee S, Chen P, Schnapp L Role of IGF-1 pathway in lung fibroblast activation. Respir Res, 2013;14(0):102. 2013 [PMID: 24103846] (cell culture, Mouse)
• Tucker, Budd A, Mullins, Robert F, Streb, Luan M, Anfinson, Kristin, Eyestone, Mari E, Kaalberg, Emily, Riker, Megan J, Drack, Arlene V, Braun, Terry A, Stone, Edwin M Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa. Elife, 2013;2(0):e00824. 2013 [PMID: 23991284] (Bioassay, Human)
• Liu Y, Weick J, Liu H, Krencik R, Zhang X, Ma L, Zhou G, Ayala M, Zhang S Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits. Nat Biotechnol, 2013;31(5):440-7. 2013 [PMID: 23604284] (Bioassay, Human)
• Buchholz, David E, Pennington, Britney, Croze, Roxanne, Hinman, Cassidy, Coffey, Peter J, Clegg, Dennis O Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium. Stem Cells Transl Med, 2013;2(5):384-93. 2013 [PMID: 23599499] (Bioassay, Human)
• Bell A, Satchwell T, Heesom K, Hawley B, Kupzig S, Hazell M, Mushens R, Herman A, Toye A Protein distribution during human erythroblast enucleation in vitro. PLoS ONE, 2013;8(4):e60300. 2013 [PMID: 23565219] (cell culture, Human)
• Cardillo T, Trisal P, Arrojo R, Goldenberg D, Chang C Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro. BMC Cancer, 2013;13(0):170. 2013 [PMID: 23548153] (Bioassay, Human)
• Wolf A, Rietscher K, Glass M, Huttelmaier S, Schutkowski M, Ihling C, Sinz A, Wingenfeld A, Mun A, Hatzfeld M Insulin signaling via Akt2 switches plakophilin 1 function from stabilizing cell adhesion to promoting cell proliferation. J Cell Sci, 2013;126(0):1832-44. 2013 [PMID: 23444369] (Bioassay, Human)
• Logan J, Sophocleous A, Marino S, Muir M, Brunton V, Idris A Selective tyrosine kinase inhibition of insulin-like growth factor-1 receptor inhibits human and mouse breast cancer-induced bone cell activity, bone remodeling, and osteolysis. J Bone Miner Res, 2013;28(5):1229-42. 2013 [PMID: 23239200] (Bioassay, Mouse)
• Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells. Mol Ther, 2013;21(1):228-39. 2013 [PMID: 23183535] (Bioassay, Human)
• Zelinka C, Scott M, Volkov L, Fischer A The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1. PLoS ONE, 2012;7(9):e44477. 2012 [PMID: 22973454] (In Vivo, Chicken)
• Aad, Pauline, Echternkamp, Sherrill, Sypherd, David D, Schreiber, Nicole B, Spicer, Leon J The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems. Biol Reprod, 2012;87(4):79. 2012 [PMID: 22811575] (Bioassay, Bovine)
• Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. Stem Cells, 2012;30(7):1455-64. 2012 [PMID: 22495904] (Bioassay, Human)
• Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 2012;21(6):1321-39. 2012 [PMID: 22195604] (cell culture, Human)
• Laranjeira AB, de Vasconcellos JF, Sodek L, Spago MC, Fornazim MC, Tone LG, Brandalise SR, Nowill AE, Yunes JA IGFBP7 participates in the reciprocal interaction between acute lymphoblastic leukemia and BM stromal cells and in leukemia resistance to asparaginase. Leukemia, 2011;26(5):1001-11. 2011 [PMID: 22005787] (Bioassay, Human)
• Iozzo RV, Buraschi S, Genua M Decorin antagonizes IGF receptor I (IGF-IR) function by interfering with IGF-IR activity and attenuating downstream signaling. J. Biol. Chem., 2011;286(40):34712-21. 2011 [PMID: 21840990] (Bioassay, Human)
• Meuillet EJ, Zuohe S, Lemos R, Ihle N, Kingston J, Watkins R, Moses SA, Zhang S, Du-Cuny L, Herbst R, Jacoby JJ, Zhou LL, Ahad AM, Mash EA, Kirkpatrick DL, Powis G Molecular pharmacology and antitumor activity of PHT-427, a novel Akt/phosphatidylinositide-dependent protein kinase 1 pleckstrin homology domain inhibitor. Mol. Cancer Ther., 2010;9(3):706-17. 2010 [PMID: 20197390] (Bioassay, Human)
• Fischer AJ, Scott MA, Zelinka C, Sherwood P A novel type of glial cell in the retina is stimulated by insulin-like growth factor 1 and may exacerbate damage to neurons and Muller glia. Glia, 2010;58(6):633-49. 2010 [PMID: 19941335] (In Vivo, Chicken)
• Banziger-Tobler NE, Halin C, Kajiya K, Detmar M Growth hormone promotes lymphangiogenesis. Am. J. Pathol., 2008;173(2):586-97. 2008 [PMID: 18583315] (WB Ctrl, N/A)
• Piao W, Wang Y, Adachi Y, Yamamoto H, Li R, Imsumran A, Li H, Maehata T, Ii M, Arimura Y, Lee CT, Shinomura Y, Carbone DP, Imai K Insulin-like growth factor-I receptor blockade by a specific tyrosine kinase inhibitor for human gastrointestinal carcinomas. Mol. Cancer Ther., 2008;7(6):1483-93. 2008 [PMID: 18566219] (Bioassay, Human)
• Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Cell Stem Cell, 2008;2(6):602-12. 2008 [PMID: 18522853] (Bioassay, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008;7(6):2406-14. 2008 [PMID: 18422355] (ELISA Standard, N/A)
• Southwood M, Jeffery TK, Yang X, Upton PD, Hall SM, Atkinson C, Haworth SG, Stewart S, Reynolds PN, Long L, Trembath RC, Morrell NW Regulation of bone morphogenetic protein signalling in human pulmonary vascular development. J. Pathol., 2008;214(1):85-95. 2008 [PMID: 17992660] (Bioassay, Human)
• Wanderling S, Simen BB, Ostrovsky O, Ahmed NT, Vogen SM, Gidalevitz T, Argon Y GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion. Mol. Biol. Cell, 2007;18(10):3764-75. 2007 [PMID: 17634284] (Bioassay, Mouse)
• Spicer LJ, Aad PY Insulin-like growth factor (IGF) 2 stimulates steroidogenesis and mitosis of bovine granulosa cells through the IGF1 receptor: role of follicle-stimulating hormone and IGF2 receptor. Biol. Reprod., 2007;77(1):18-27. 2007 [PMID: 17360960] (Bioassay, Bovine)
• Sheen-Chen SM, Ho HT, Chia-Pei L, Hung KS, Eng HL The effect of insulin-like growth factor-I on hepatocyte apoptosis after bile duct ligation in rat. Dig. Dis. Sci., 2006;51(12):2220-4. 2006 [PMID: 17103039] (In Vivo, Rat)
• Bartunek J, Croissant JD, Wijns W, Gofflot S, de Lavareille A, Vanderheyden M, Kaluzhny Y, Mazouz N, Willemsen P, Penicka M, Mathieu M, Homsy C, De Bruyne B, McEntee K, Lee IW, Heyndrickx GR Pretreatment of adult bone marrow mesenchymal stem cells with cardiomyogenic growth factors and repair of the chronically infarcted myocardium. Am. J. Physiol. Heart Circ. Physiol., 2006;292(2):H1095-104. 2006 [PMID: 17056665] (Bioassay, Canine)
• Anderson BC, Christiansen SP, Grandt S, Grange RW, McLoon LK Increased extraocular muscle strength with direct injection of insulin-like growth factor-I. Invest. Ophthalmol. Vis. Sci., 2006;47(6):2461-7. 2006 [PMID: 16723457] (In Vivo, Rabbit)
• Li Y, Fan J, Chen M, Li W, Woodley DT Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. J. Invest. Dermatol., 2006;126(9):2096-105. 2006 [PMID: 16691197] (Bioassay, Human)
• Spicer LJ, Aad PY, Allen D, Mazerbourg S, Hsueh AJ Growth differentiation factor-9 has divergent effects on proliferation and steroidogenesis of bovine granulosa cells. J. Endocrinol., 2006;189(2):329-39. 2006 [PMID: 16648300] (Bioassay, Bovine)
• Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells. Glia, 2006;53(8):845-57. 2006 [PMID: 16586492] (Bioassay, Human)
• Larsen PH, DaSilva AG, Conant K, Yong VW Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice. J. Neurosci., 2006;26(8):2207-14. 2006 [PMID: 16495447] (Bioassay, Mouse)
• Hanley MB, Napolitano LA, McCune JM Growth hormone-induced stimulation of multilineage human hematopoiesis. Stem Cells, 2005;23(8):1170-9. 2005 [PMID: 15955828] (Bioassay, Human)
• Escartin C, Boyer F, Bemelmans AP, Hantraye P, Brouillet E Insulin growth factor-1 protects against excitotoxicity in the rat striatum. Neuroreport, 2004;15(14):2251-4. 2004 [PMID: 15371744] (In Vivo, Rat)
• Gahr SA, Kocamis H, Richter JJ, Killefer J The effects of in ovo rhIGF-I administration on expression of the growth hormone secretagogue receptor (GHSR) during chicken embryonic development. Growth Dev Aging, 2004;68(1):3-10. 2004 [PMID: 15307660] (In Vivo, Chicken)
• Brenner N, Huning M, Stange R Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing. Calcif. Tissue Int., 2004;74(4):388-97. 2004 [PMID: 15255077] (In Vivo, Rat)
• Wildemann B, Bamdad P, Holmer Ch, Haas NP, Raschke M, Schmidmaier G Local delivery of growth factors from coated titanium plates increases osteotomy healing in rats. Bone, 2004;34(5):862-8. 2004 [PMID: 15121018] (In Vivo, Rat)
• Studer RK, Bergman R, Stubbs T, Decker K Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition. Arthritis Res. Ther., 2004;6(1):R56-R64. 2004 [PMID: 14979938] (Bioassay, Rabbit)
• Oropeza A, Wrenzycki C, Herrmann D, Hadeler KG, Niemann H Improvement of the developmental capacity of oocytes from prepubertal cattle by intraovarian insulin-like growth factor-I application. Biol. Reprod., 2004;70(6):1634-43. 2004 [PMID: 14766727] (In Vivo, Bovine)
• Loeser RF, Carlson CS, Del Carlo M, Cole A Detection of nitrotyrosine in aging and osteoarthritic cartilage: Correlation of oxidative damage with the presence of interleukin-1beta and with chondrocyte resistance to insulin-like growth factor 1. Arthritis Rheum., 2002;46(9):2349-57. 2002 [PMID: 12355482] (Bioassay, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications. Oncogene, 2002;21(37):5673-83. 2002 [PMID: 12173037] (Bioassay, Human)
• Derevjanik NL, Vinores SA, Xiao WH, Mori K, Turon T, Hudish T, Dong S, Campochiaro PA Quantitative assessment of the integrity of the blood-retinal barrier in mice. Invest. Ophthalmol. Vis. Sci., 2002;43(7):2462-7. 2002 [PMID: 12091451] (In Vivo, Mouse)
• Loeser RF, 2018, Shanker G, Carlson CS, Gardin JF, Shelton BJ, Sonntag WE Reduction in the chondrocyte response to insulin-like growth factor 1 in aging and osteoarthritis: studies in a non-human primate model of naturally occurring disease. Arthritis Rheum., 2000;43(9):2110-20. 2000 [PMID: 11014363] (Bioassay, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Deleu S, Pirson I, Clermont F, Nakamura T, Dumont JE, Maenhaut C Immediate early gene expression in dog thyrocytes in response to growth, proliferation, and differentiation stimuli. J. Cell. Physiol., 1999;181(2):342-54. 1999 [PMID: 10497313] (Bioassay, Canine)

Bioinformatics

• Gene Symbol: IGF1
• Uniprot:
  • P05019()