RUNX2/CBFA1 Antibody (232902) [Unconjugated]

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RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
232902
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

RUNX2/CBFA1 Antibody (232902) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys233-Tyr418
Accession # Q13950
Specificity
Detects human RUNX2/CBFA1.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
RUNX2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB2006 in the following application:

Publications
Read Publications using
MAB2006 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RUNX2/CBFA1 Antibody (232902) [Unconjugated]

  • Acute myeloid leukemia 3 protein
  • CBFA1
  • CBF-alpha-1
  • CCD1
  • CCDAML3
  • CLCD
  • Core-binding factor subunit alpha-1
  • core-binding factor, runt domain, alpha subunit 1
  • MGC120023
  • ML3
  • oncogene AML-3
  • OSF2
  • OSF-2
  • osteoblast-specific transcription factor 2
  • PEA2aA
  • PEA2-alpha A
  • PEBP2A
  • PEBP2aA
  • PEBP2-alpha A
  • polyomavirus enhancer-binding protein 2 alpha A subunit
  • runt domain, alpha subunit 1
  • runt related transcription factor 2
  • runt-related transcription factor 2
  • RUNX2
  • SL3/AKV core-binding factor alpha A subunit
  • SL3-3 enhancer factor 1 alpha A subunit

Background

CBFA1, also called runt-related transcription factor 2 (RUNX2), is an essential transcription factor for the regulation of osteoblast differentiation (1). The CBFA1 gene potentially encodes several proteins that differ in their N-terminal sequences and transactivation capacities (2).

  1. Ducy, P. et al. (1997) Cell 89:747.
  2. Xiao, Z.S. et al. (1998) Gene 214:187.
  3. Sato, M. et al. (1998) Oncogene 17:1517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RUNX2/CBFA1 Antibody (MAB2006)(27)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


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Flow Cytometry
(1)
ICC
(3)
IHC
(7)
IHC-Fr
(3)
IHC-P
(5)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using MAB2006 Applications Species
Q Song, Y Yang, D Jiang, Z Qin, C Xu, H Wang, J Huang, L Chen, R Luo, X Zhang, Y Huang, L Xu, Z Yu, S Tan, M Deng, R Xue, J Qie, K Li, Y Yin, X Yue, X Sun, J Su, F He, C Ding, Y Hou Proteomic analysis reveals key differences between squamous cell carcinomas and adenocarcinomas across multiple tissues Oncogene, 2022-07-18;13(1):4167. 2022-07-18 [PMID: 35851595] (IHC, Human) IHC Human
C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams Induction of osteogenesis by bone-targeted Notch activation Elife, 2022-02-04;11(0):. 2022-02-04 [PMID: 35119364] (IHC, Mouse) IHC Mouse
Francesca Marta Elli, Deborah Mattinzoli, Camilla Lucca, Matteo Piu, Maria A. Maffini, Jole Costanza et al. Novel Pathogenetic Variants in PTHLH and TRPS1 Genes Causing Syndromic Brachydactyly Journal of Bone and Mineral Research 2022-03-01 [PMID: 34897794]
M Minguzzi, V Panichi, S D'Adamo, S Cetrullo, L Cattini, F Flamigni, E Mariani, RM Borzì Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes International Journal of Molecular Sciences, 2021-11-05;22(21):. 2021-11-05 [PMID: 34769441] (IHC, Human) IHC Human
Akitoshi Hara, Katsuhiro Kato, Toshikazu Ishihara, Hiroki Kobayashi, Naoya Asai, Shinji Mii et al. Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity Genes to Cells 2021-07-01 [PMID: 33960573]
J Yao, X Wu, X Qiao, D Zhang, L Zhang, JA Ma, X Cai, KI Boström, Y Yao Shifting osteogenesis in vascular calcification JCI Insight, 2021-05-24;0(0):. 2021-05-24 [PMID: 33848269] (Western Blot, Mouse) Western Blot Mouse
Oe Y, Mitsui S, Sato E, Shibata N, Kisu K, Sekimoto A, Miyazaki M, Sato H, Ito S, Takahashi N Lack of Endothelial Nitric Oxide Synthase Accelerates Ectopic Calcification in Uremic Mice Fed an Adenine and High Phosphorus Diet. Am J Pathol, 2020-11-04;191(2):283-293. 2020-11-04 [PMID: 33159888] (IHC, Mouse) IHC Mouse
S Supakul, K Yao, H Ochi, T Shimada, K Hashimoto, S Sunamura, Y Mabuchi, M Tanaka, C Akazawa, T Nakamura, A Okawa, S Takeda, S Sato Pericytes as a Source of Osteogenic Cells in Bone Fracture Healing Int J Mol Sci, 2019-03-02;20(5):. 2019-03-02 [PMID: 30832329] (IHC-Fr, Mouse) IHC-Fr Mouse
AGE Day, WR Francis, K Fu, IL Pieper, O Guy, Z Xia Osteogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells on Coralline Hydroxyapatite/Calcium Carbonate Microparticles Stem Cells Int, 2018-09-05;2018(0):4258613. 2018-09-05 [PMID: 30254682] (IHC, Human) IHC Human
Jiewen Dai, Jiawen Si, Ningjuan Ouyang, Jianfei Zhang, Dandan Wu, Xudong Wang et al. Dental and periodontal phenotypes of Dlx2 overexpression in mice Molecular Medicine Reports 2017-05-01 [PMID: 28447749]
Show All 27 Publications.

Review for RUNX2/CBFA1 Antibody (MAB2006) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2006:
Filter by Applications
Flow
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
Flow Human 02/16/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 22034088
SpeciesHuman

FAQs for RUNX2/CBFA1 Antibody (MAB2006). (Showing 1 - 1 of 1 FAQs).

  1. We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.
    • We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.
mFluor Violet Conjugated Antibodies

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Verified Customer
02/16/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol RUNX2
Uniprot