RUNX2/CBFA1 Antibody (232902) [Unconjugated]

Images

 
RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
232902
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

RUNX2/CBFA1 Antibody (232902) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys233-Tyr418
Accession # Q13950
Specificity
Detects human RUNX2/CBFA1.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
RUNX2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB2006 in the following application:

Publications
Read Publications using
MAB2006 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RUNX2/CBFA1 Antibody (232902) [Unconjugated]

  • Acute myeloid leukemia 3 protein
  • CBFA1
  • CBF-alpha-1
  • CCD1
  • CCDAML3
  • CLCD
  • Core-binding factor subunit alpha-1
  • core-binding factor, runt domain, alpha subunit 1
  • MGC120023
  • ML3
  • oncogene AML-3
  • OSF2
  • OSF-2
  • osteoblast-specific transcription factor 2
  • PEA2aA
  • PEA2-alpha A
  • PEBP2A
  • PEBP2aA
  • PEBP2-alpha A
  • polyomavirus enhancer-binding protein 2 alpha A subunit
  • runt domain, alpha subunit 1
  • runt related transcription factor 2
  • runt-related transcription factor 2
  • RUNX2
  • SL3/AKV core-binding factor alpha A subunit
  • SL3-3 enhancer factor 1 alpha A subunit

Background

CBFA1, also called runt-related transcription factor 2 (RUNX2), is an essential transcription factor for the regulation of osteoblast differentiation (1). The CBFA1 gene potentially encodes several proteins that differ in their N-terminal sequences and transactivation capacities (2).

  1. Ducy, P. et al. (1997) Cell 89:747.
  2. Xiao, Z.S. et al. (1998) Gene 214:187.
  3. Sato, M. et al. (1998) Oncogene 17:1517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RUNX2/CBFA1 Antibody (MAB2006)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(3)
IHC
(4)
IHC-Fr
(3)
IHC-P
(5)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(12)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using MAB2006 Applications Species
J Yao, X Wu, X Qiao, D Zhang, L Zhang, JA Ma, X Cai, KI Boström, Y Yao Shifting osteogenesis in vascular calcification JCI Insight, 2021;0(0):. 2021 [PMID: 33848269] (Western Blot, Mouse) Western Blot Mouse
Oe Y, Mitsui S, Sato E, Shibata N, Kisu K, Sekimoto A, Miyazaki M, Sato H, Ito S, Takahashi N Lack of Endothelial Nitric Oxide Synthase Accelerates Ectopic Calcification in Uremic Mice Fed an Adenine and High Phosphorus Diet. Am J Pathol, 0;191(2):283-293. 0 [PMID: 33159888] (IHC, Mouse) IHC Mouse
S Supakul, K Yao, H Ochi, T Shimada, K Hashimoto, S Sunamura, Y Mabuchi, M Tanaka, C Akazawa, T Nakamura, A Okawa, S Takeda, S Sato Pericytes as a Source of Osteogenic Cells in Bone Fracture Healing Int J Mol Sci, 2019;20(5):. 2019 [PMID: 30832329] (IHC-Fr, Mouse) IHC-Fr Mouse
AGE Day, WR Francis, K Fu, IL Pieper, O Guy, Z Xia Osteogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells on Coralline Hydroxyapatite/Calcium Carbonate Microparticles Stem Cells Int, 2018;2018(0):4258613. 2018 [PMID: 30254682] (IHC, Human) IHC Human
G Lisignoli, E Lambertini, C Manferdini, E Gabusi, L Penolazzi, F Paolella, M Angelozzi, V Casagranda, R Piva Collagen type XV and the 'osteogenic status' J. Cell. Mol. Med, 2017;0(0):. 2017 [PMID: 28332281] (Flow Cytometry, Human) Flow Cytometry Human
UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017;19(3):189-201. 2017 [PMID: 28218908] (IHC, Mouse) IHC Mouse
P Leucht The Selective Serotonin Re-Uptake Inhibitor Fluoxetine Directly Inhibits Osteoblast Differentiation and Mineralization During Fracture Healing in Mice J. Bone Miner. Res., 2016;0(0):. 2016 [PMID: 27869327] (IHC-P, Mouse) IHC-P Mouse
Guemes M, Garcia A, Rigueur D, Runke S, Wang W, Zhao G, Mayorga V, Atti E, Tetradis S, Peault B, Lyons K, Miranda-Carboni G, Krum S GATA4 is essential for bone mineralization via ERalpha and TGFbeta/BMP pathways. J Bone Miner Res, 2014;29(12):2676-87. 2014 [PMID: 24932701] (IHC-P, Mouse) IHC-P Mouse
Ramasamy S, Kusumbe A, Wang L, Adams R Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Nature, 2014;507(7492):376-80. 2014 [PMID: 24647000] (IHC-Fr, Mouse) IHC-Fr Mouse
Kusumbe A, Ramasamy S, Adams R Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature, 2014;507(7492):323-8. 2014 [PMID: 24646994] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 17 Publications.

Review for RUNX2/CBFA1 Antibody (MAB2006) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2006:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
Flow Human 02/16/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 22034088
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22034088

Details

Detection NotesSee PMID 22034088

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22034088

FAQs for RUNX2/CBFA1 Antibody (MAB2006). (Showing 1 - 1 of 1 FAQs).

  1. We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.
    • We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.

Secondary Antibodies

 

Isotype Controls

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Verified Customer
02/16/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol RUNX2
Uniprot