RUNX2/CBFA1 Antibody (232902) [Unconjugated]

Images

 
RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC
Clone
232902
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

RUNX2/CBFA1 Antibody (232902) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys233-Tyr418
Accession # Q13950
Specificity
Detects human RUNX2/CBFA1.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
RUNX2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB2006 in the following application:

Publications
Read Publications using
MAB2006 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RUNX2/CBFA1 Antibody (232902) [Unconjugated]

  • Acute myeloid leukemia 3 protein
  • AML3CCD
  • CBFA1
  • CBFA1MGC120022
  • Core-binding factor subunit alpha-1
  • MGC120023
  • PEA2-alpha A
  • PEBP2A
  • PEBP2A1CCD1
  • PEBP2aA1
  • PEBP2-alpha A
  • runt domain, alpha subunit 1
  • runt-related transcription factor 2
  • RUNX2
  • SL3/AKV core-binding factor alpha A subunit
  • SL3-3 enhancer factor 1 alpha A subunit

Background

CBFA1, also called runt-related transcription factor 2 (RUNX2), is an essential transcription factor for the regulation of osteoblast differentiation (1). The CBFA1 gene potentially encodes several proteins that differ in their N-terminal sequences and transactivation capacities (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RUNX2/CBFA1 Antibody (MAB2006)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow, ICC, IHC, IHC Fluorescence, IHC Frozen, IHC Paraffin-embedded.


Filter By Application
Flow
(1)
ICC
(3)
IHC
(1)
IHC Fluorescence
(1)
IHC Frozen
(2)
IHC Paraffin-embedded
(4)
All Applications
Filter By Species
Human
(4)
Mouse
(8)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB2006 Applications Species
G Lisignoli, E Lambertini, C Manferdini, E Gabusi, L Penolazzi, F Paolella, M Angelozzi, V Casagranda, R Piva Collagen type XV and the 'osteogenic status' J. Cell. Mol. Med, 2017;0(0):. 2017 [PMID: 28332281] (Flow,, Human) Flow Human
UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017;19(3):189-201. 2017 [PMID: 28218908] (IHC,, Mouse) IHC Mouse
Guemes M, Garcia A, Rigueur D, Runke S, Wang W, Zhao G, Mayorga V, Atti E, Tetradis S, Peault B, Lyons K, Miranda-Carboni G, Krum S GATA4 is essential for bone mineralization via ERalpha and TGFbeta/BMP pathways. J Bone Miner Res, 2014;29(12):2676-87. 2014 [PMID: 24932701] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Ramasamy S, Kusumbe A, Wang L, Adams R Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Nature, 2014;507(7492):376-80. 2014 [PMID: 24647000] (IHC Frozen,, Mouse) IHC Frozen Mouse
Kusumbe A, Ramasamy S, Adams R Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature, 2014;507(7492):323-8. 2014 [PMID: 24646994] (IHC Frozen,, Mouse) IHC Frozen Mouse
Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R Identification of a clonally expanding haematopoietic compartment in bone marrow. EMBO J, 2013;32(2):219-30. 2013 [PMID: 23188081] (IHC Fluorescence,, Mouse) IHC Fluorescence Mouse
Pai A, Leaf EM, El-Abbadi M, Giachelli CM Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease. Am. J. Pathol., 2011;178(2):764-73. 2011 [PMID: 21281809] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Todoerti K, Lisignoli G, Storti P, Agnelli L, Novara F, Manferdini C, Codeluppi K, Colla S, Crugnola M, Abeltino M, Bolzoni M, Sgobba V, Facchini A, Lambertenghi-Deliliers G, Zuffardi O, Rizzoli V, Neri A, Giuliani N Distinct transcriptional profiles characterize bone microenvironment mesenchymal cells rather than osteoblasts in relationship with multiple myeloma bone disease. Exp. Hematol., 2010;38(2):141-53. 2010 [PMID: 19963035] (ICC,, Human) ICC Human
El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin. Kidney Int., 2009;75(12):1297-307. 2009 [PMID: 19322138] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Ruiz SA, Chen CS Emergence of patterned stem cell differentiation within multicellular structures. Stem Cells, 2008;26(11):2921-7. 2008 [PMID: 18703661] (ICC,, Human) ICC Human
Show All 12 Publications.

Review for RUNX2/CBFA1 Antibody (MAB2006) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2006:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 02/16/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 22034088
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22034088

Details

Detection NotesSee PMID 22034088

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22034088

FAQs for RUNX2/CBFA1 Antibody (MAB2006). (Showing 1 - 1 of 1 FAQs).

  1. We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.
    • We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.

Secondary Antibodies

 

Isotype Controls

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Diseases for RUNX2/CBFA1 Antibody (MAB2006)

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Anonymous
02/16/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol RUNX2
Entrez
Uniprot