PTH Antibody (BGN/1F8) - BSA Free

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: BGN/1F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PTH Antibody (BGN/1F8) - BSA Free Summary

• Description: Novus Biologicals Mouse PTH Antibody (BGN/1F8) - BSA Free (NB100-65676) is a monoclonal antibody validated for use in IHC, ELISA and ICC/IF. Anti-PTH Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acids 1-34 of mature PTH conjugated to a proprietary carrier molecule
• Specificity: 2 x 10^7with 1-34 PTH, 5 x 10^8 with 1-84 PTH
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27537691)

Reactivity Notes

Predicted cross-reactivities: Mouse, Rat

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for PTH Antibody (BGN/1F8) - BSA Free

• Parathormone
• Parathyrin
• parathyroid hormone 1
• parathyroid hormone
• PTH
• PTH1

Background

Parathyroid hormone is secreted by parathyroid cells. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH). Most PTH is secreted in its intact, mature 9.5 kDa form, amino acids 1-84; however, it can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. No PTH fragment has been found to be the result of another gene or alternative splicing of the PTH gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTH Antibody (NB100-65676)(2)

Publications using NB100-65676

• Ponnapakkam T, Anbalagan M, Stratford RE Jr et al. Novel bone-targeted parathyroid hormone-related peptide antagonists inhibit breast cancer bone metastases Anticancer Drugs 2021-02-17 [PMID: 33595947]
  
  Details:
  Citation using the HRP format of this antibody.
• Koh J, Hogue JA, Sosa JA. A Novel Ex Vivo Method for Visualizing Live-Cell Calcium Response Behavior in Intact Human Tumors. PLoS One 2016-08-18 [PMID: 27537691] (ICC/IF)

FAQs for PTH Antibody (NB100-65676). (Showing 1 - 1 of 1 FAQs).

1. What carrier molecule is conjugated to the immunogen of this PTH antibody (NB100-65676)? How do I know what to use for blocking? As I typically use BSA, could you recommend a blocking agent if to use this antibody for an ELISA?
   • Unfortunately the blocking agent is proprietary, but the carrier molecule is not BSA so you can block as you normally would.

Bioinformatics

• Gene Symbol: PTH
• Entrez:
  • 5741(Human)
• Uniprot:
  • P01270()
  • P01270()